Restriction map for
pFN21AE0362
(Circular) (Six-base) MAPSORT of: pFN21AE0362.seq Check: 8751 from: 1 to: 6295
pFN21AE0362 6295 bp
With 182 enzymes: SgfI *
April 15, 2010 09:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3423 3423
Size: 6295
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4105 278
Size: 53 83 186 936 2569 2468
Fragments arranged by size:
2569 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2050 3638 1937
Size: 113 1588 4594
Fragments arranged by size:
4594 1588 113
Acc65I G'GTAC_C
Cuts at: 3444 3616 3444
Size: 172 6123
AclI AA'CG_TT
Cuts at: 1563 4422 4795 1563
Size: 2859 373 3063
Fragments arranged by size:
3063 2859 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3276 4358 5742 6010 1632
Size: 1644 1082 1384 268 1917
Fragments arranged by size:
1917 1644 1384 1082 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6098
AflIII A'CryG_T
Cuts at: 1203 5194 6171 1203
Size: 3991 977 1327
Fragments arranged by size:
3991 1327 977
AgeI A'CCGG_T
Cuts at: 5126 5126
Size: 6295
AhdI GACnn_n'nnGTC
Cuts at: 5024 5024
Size: 6295
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6295
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6295
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2255 2287 2255
Size: 32 6263
AlwNI CAG_nnn'CTG
Cuts at: 4038 5610 4038
Size: 1572 4723
ApaLI G'TGCA_C
Cuts at: 2544 4352 5508 2544
Size: 1808 1156 3331
Fragments arranged by size:
3331 1808 1156
ApoI r'AATT_y
Cuts at: 1493 2703 2861 2934 3604 3830 3982 6091 1493
Size: 1210 158 73 670 226 152 2109 1697
Fragments arranged by size:
2109 1697 1210 670 226 158 152 73
AscI GG'CGCG_CC
Cuts at: 3405 3405
Size: 6295
AseI AT'TA_AT
Cuts at: 160 1032 2877 4849 160
Size: 872 1845 1972 1606
Fragments arranged by size:
1972 1845 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6295
AvaI C'yCGr_G
Cuts at: 1870 1943 3620 1870
Size: 73 1677 4545
Fragments arranged by size:
4545 1677 73
BamHI G'GATC_C
Cuts at: 3625 3992 3625
Size: 367 5928
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2302 3444 3616 5071 618
Size: 325 217 627 515 1142 172 1455 1842
Fragments arranged by size:
1842 1455 1142 627 515 325 217 172
BanII G_rGCy'C
Cuts at: 1391 3614 1391
Size: 2223 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2234 2497 928
Size: 971 335 263 4726
Fragments arranged by size:
4726 971 335 263
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4486 4520 1903
Size: 34 2549 34 3678
Fragments arranged by size:
3678 2549 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2796 4187 5397 2796
Size: 1391 1210 3694
Fragments arranged by size:
3694 1391 1210
BclI T'GATC_A
Cuts at: 1548 3181 1548
Size: 1633 4662
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4906 136
Size: 107 122 71 631 605 3234 1525
Fragments arranged by size:
3234 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6290 6290
Size: 6295
BlpI GC'TnA_GC
Cuts at: 3712 3712
Size: 6295
Bme1580I G_kGCm'C
Cuts at: 1790 2100 2548 2555 4356 5512 1790
Size: 310 448 7 1801 1156 2573
Fragments arranged by size:
2573 1801 1156 448 310 7
BmgBI CAC'GTC
Cuts at: 1318 3466 1318
Size: 2148 4147
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2210 4026 4984
Size: 416 53 83 399 24 793 1816 958
Cuts at: 4984 442
Size: 1753
Fragments arranged by size:
1816 1753 958 793 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6295
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2273 2907 3243 3310 4955 1239
Size: 1034 634 336 67 1645 2579
Fragments arranged by size:
2579 1645 1034 634 336 67
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4420 5285 5583 4420
Size: 865 298 5132
Fragments arranged by size:
5132 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2932 4958 882
Size: 617 1433 2026 2219
Fragments arranged by size:
2219 2026 1433 617
BsaAI yAC'GTr
Cuts at: 493 1204 4086 493
Size: 711 2882 2702
Fragments arranged by size:
2882 2702 711
BsaBI GATnn'nnATC
Cuts at: 6289 6289
Size: 6295
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2601 4102 4484 5119
Size: 53 83 186 936 1068 1501 382 635
Cuts at: 5119 275
Size: 1451
Fragments arranged by size:
1501 1451 1068 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3537 4728 5126 5400 5547 5958 3537
Size: 1191 398 274 147 411 3874
Fragments arranged by size:
3874 1191 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3210 3240 1178
Size: 30 165 30 1807 30 4233
Fragments arranged by size:
4233 1807 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2550 1186
Size: 1364 4931
BseYI C'CCAG_C
Cuts at: 5498 5994 5498
Size: 496 5799
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2034 1163
Size: 573 298 5424
Fragments arranged by size:
5424 573 298
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3745 4506 5534 1540
Size: 275 186 1744 761 1028 2301
Fragments arranged by size:
2301 1744 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2548 4356 4441 5512 2548
Size: 1808 85 1071 3331
Fragments arranged by size:
3331 1808 1071 85
BsmI GAATG_Cn'
Cuts at: 3806 3899 3806
Size: 93 6202
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2100 2548 2555 3614 4356 4441 5512
Size: 399 310 448 7 1059 742 85 1071
Cuts at: 5512 1391
Size: 2174
Fragments arranged by size:
2174 1071 1059 742 448 399 310 85
7
BspEI T'CCGG_A
Cuts at: 5958 5958
Size: 6295
BspHI T'CATG_A
Cuts at: 4184 5914 4184
Size: 1730 4565
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3423 3650 844
Size: 2579 227 3489
Fragments arranged by size:
3489 2579 227
BsrBI CCG'CTC
Cuts at: 1394 1823 4182 1394
Size: 429 2359 3507
Fragments arranged by size:
3507 2359 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2306 3151 4790 4964 65
Size: 1183 1058 845 1639 174 1396
Fragments arranged by size:
1639 1396 1183 1058 845 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4939 5126 1700
Size: 110 3129 187 2869
Fragments arranged by size:
3129 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6295
BssHII G'CGCG_C
Cuts at: 1925 3405 1925
Size: 1480 4815
BssSI C'ACGA_G
Cuts at: 2010 4355 5367 2010
Size: 2345 1012 2938
Fragments arranged by size:
2938 2345 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6295
BstBI TT'CG_AA
Cuts at: 5099 5099
Size: 6295
BstEII G'GTnAC_C
Cuts at: 1184 3080 1184
Size: 1896 4399
BstYI r'GATC_y
Cuts at: 1972 3625 3992 4377 4394 5846 5932 5944 6290
Size: 1653 367 385 17 1452 86 12 346
Cuts at: 6290 1972
Size: 1977
Fragments arranged by size:
1977 1653 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2153 2153
Size: 6295
BtgI C'CryG_G
Cuts at: 513 1065 2004 2100 3121 3156 513
Size: 552 939 96 1021 35 3652
Fragments arranged by size:
3652 1021 939 552 96 35
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5556
Fragments arranged by size:
5556 683 56
BtsI GCAGTG_nn'
Cuts at: 2442 3811 4605 4625 2442
Size: 1369 794 20 4112
Fragments arranged by size:
4112 1369 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6295
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6260
DraI TTT'AAA
Cuts at: 3517 3599 3517
Size: 82 6213
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5302 809
Size: 507 3986 1802
Fragments arranged by size:
3986 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3366 3483 3742
Size: 54 1425 50 275 18 1536 117 259
Cuts at: 3742 4631 6260 8
Size: 889 1629 43
Fragments arranged by size:
1629 1536 1425 889 275 259 117 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3742 1537
Size: 275 1930 4090
Fragments arranged by size:
4090 1930 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4225 1353
Size: 2872 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4882 5256 5402 387
Size: 1064 138 3293 374 146 1280
Fragments arranged by size:
3293 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2273 2907 3243 3276 3310 4358 4955
Size: 393 641 634 336 33 34 1048 597
Cuts at: 4955 5742 6010 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1048 787 641 634 597 393 336
268 34 33
EcoNI CCTnn'n_nnAGG
Cuts at: 3335 3335
Size: 6295
EcoRI G'AATT_C
Cuts at: 3604 3982 3604
Size: 378 5917
FspI TGC'GCA
Cuts at: 4801 4801
Size: 6295
HaeII r_GCGC'y
Cuts at: 1678 5442 1678
Size: 3764 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2910 2942 4936 4968 5010
Size: 32 135 32 1338 32 1994 32 42
Cuts at: 5010 5042 1373
Size: 32 2626
Fragments arranged by size:
2626 1994 1338 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3639 3885 669
Size: 988 281 1701 246 3079
Fragments arranged by size:
3079 1701 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6295
HpaI GTT'AAC
Cuts at: 3885 3885
Size: 6295
KpnI G_GTAC'C
Cuts at: 3448 3620 3448
Size: 172 6123
MfeI C'AATT_G
Cuts at: 2958 3894 2958
Size: 936 5359
MluI A'CGCG_T
Cuts at: 6171 6171
Size: 6295
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5409 5593 1305
Size: 4104 184 2007
Fragments arranged by size:
4104 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4473
Fragments arranged by size:
4473 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3161 4253 4612 4771 518
Size: 648 1995 1092 359 159 2042
Fragments arranged by size:
2042 1995 1092 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3358 3711 4388 5536 5781 1606
Size: 1752 353 677 1148 245 2120
Fragments arranged by size:
2120 1752 1148 677 353 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2100 3121 3156 513
Size: 552 939 96 1021 35 3652
Fragments arranged by size:
3652 1021 939 552 96 35
NdeI CA'TA_TG
Cuts at: 387 2728 3140 387
Size: 2341 412 3542
Fragments arranged by size:
3542 2341 412
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6295
NotI GC'GGCC_GC
Cuts at: 3742 3742
Size: 6295
NspI r_CATG'y
Cuts at: 1132 1229 3653 5198 1132
Size: 97 2424 1545 2229
Fragments arranged by size:
2424 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6295
PciI A'CATG_T
Cuts at: 5194 5194
Size: 6295
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3460 1108
Size: 2352 3943
PmeI GTTT'AAAC
Cuts at: 3599 3599
Size: 6295
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4364 4396 5877 5909 4364
Size: 32 1481 32 4750
Fragments arranged by size:
4750 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6295
PsiI TTA'TAA
Cuts at: 3865 3865
Size: 6295
PstI C_TGCA'G
Cuts at: 830 3647 830
Size: 2817 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6295
SalI G'TCGA_C
Cuts at: 1936 3637 1936
Size: 1701 4594
SbfI CC_TGCA'GG
Cuts at: 3647 3647
Size: 6295
ScaI AGT'ACT
Cuts at: 4543 6255 4543
Size: 1712 4583
SexAI A'CCwGG_T
Cuts at: 3440 3440
Size: 6295
SfcI C'TryA_G
Cuts at: 826 1045 2737 3643 4778 5459 5650 826
Size: 219 1692 906 1135 681 191 1471
Fragments arranged by size:
1692 1471 1135 906 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6295
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6295
SmaI CCC'GGG
Cuts at: 1872 3622 1872
Size: 1750 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4399 5300 5562 820
Size: 197 926 2456 901 262 1553
Fragments arranged by size:
2456 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4086 493
Size: 3593 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6295
SphI G_CATG'C
Cuts at: 1132 3653 1132
Size: 2521 3774
SspI AAT'ATT
Cuts at: 5 52 2483 2520 4219 5
Size: 47 2431 37 1699 2081
Fragments arranged by size:
2431 2081 1699 47 37
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6295
StyI C'CwwG_G
Cuts at: 513 1065 2004 2073 2100 3121 3156 3459 3734
Size: 552 939 69 27 1021 35 303 275
Cuts at: 3734 513
Size: 3074
Fragments arranged by size:
3074 1021 939 552 303 275 69 35
27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2409 3122 3367 4322 4339 4492 4677
Size: 1255 829 713 245 955 17 153 185
Cuts at: 4677 5122 325
Size: 445 1498
Fragments arranged by size:
1498 1255 955 829 713 445 245 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4541 6253 96
Size: 274 80 33 51 1443 2564 1712 138
Fragments arranged by size:
2564 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2835 2867 3210 3242 6255 6287 2835
Size: 32 343 32 3013 32 2843
Fragments arranged by size:
3013 2843 343 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6295
XbaI T'CTAG_A
Cuts at: 3631 3631
Size: 6295
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2131 2190 2131
Size: 59 6236
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6295
XmaI C'CCGG_G
Cuts at: 1870 3620 1870
Size: 1750 4545
XmnI GAAnn'nnTTC
Cuts at: 4424 4424
Size: 6295
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4103 276
Size: 53 83 186 936 2569 2468
Fragments arranged by size:
2569 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AscI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI
SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI ApaI AvrII BaeI BbeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoO109I
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI
PsrI PvuII RsrII SacI SacII SanDI SapI SfiI
SfoI SrfI SwaI