################################## # NO CUT ENZYMES ( 124 enzymes) # ################################## AatII AccI AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AspEI AspHI AspI AvaI AviII AvrII BamHI BanII BbeI BbrPI BbvCI BcgI BciVI BfrI BglI BglII BlnI BsaAI BsaBI BsaHI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI BspDI BspEI BspHI BspLU11I BsrBI BsrFI BssSI Bst1107I BstBI BstF5I BstXI BstZ17I Bsu36I Cfr10I ClaI CpoI DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoRI EcoRV FokI FseI FspI HaeII HgaI Hin1I HincII HindII HindIII HpaI KasI KspI MamI MluI MroI NaeI NarI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspI SwaI Tth111I XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### Acc65I 1451 GGTACC G^GTACC AlwNI 114 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG AscI 1411 GGCGCGCC GG^CGCGCC AseI 883 ATTAAT AT^TAAT AsnI 883 ATTAAT AT^TAAT Asp718I 1451 GGTACC G^GTACC BbsI 820 GTCTTC GAAGAC(2/6) BcnI 1353 CCCGG CC^[GC]GG BlpI 123 GCTCAGC GC^T[ACGT]AGC Bpu1102I 123 GCTCAGC GC^T[ACGT]AGC BpuAI 820 GTCTTC GAAGAC(2/6) BsaI 933 GGTCTC GGTCTC(1/5) BsaWI 1544 ACCGGA [AT]^CCGG[AT] BseRI 55 CTCCTC GAGGAG(10/8) Bsp1407I 200 TGTACA T^GTACA BsrDI 1151 GCAATG GCAATG(2/0) BsrGI 200 TGTACA T^GTACA BssHII 1412 GCGCGC G^CGCGC BstAPI 694 GCAAGCCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 1087 GGTCACC G^GT[ACGT]ACC BstPI 1087 GGTCACC G^GT[ACGT]ACC BstYI 738 GGATCT [GA]^GATC[CT] CelII 123 GCTCAGC GC^T[ACGT]AGC DraI 1522 TTTAAA TTT^AAA EarI 211 GAAGAG CTCTTC(1/4) EcoNI 1338 CCTTCACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 1087 GGTCACC G^GT[ACGT]ACC EcoT22I 752 ATGCAT ATGCA^T KpnI 1451 GGTACC GGTAC^C Ksp632I 211 GAAGAG CTCTTC(1/4) MfeI 965 CAATTG C^AATTG MflI 738 GGATCT [GA]^GATC[CT] MunI 965 CAATTG C^AATTG NciI 1353 CCCGG CC^[GC]GG NdeI 1146 CATATG CA^TATG NsiI 752 ATGCAT ATGCA^T PleI 11 GACTC GAGTC(4/5) PpuMI 146 GGGACCT [GA]G^G[AT]CC[CT] PshBI 883 ATTAAT AT^TAAT PvuII 31 CAGCTG CAG^CTG SmlI 516 CTTGAG C^T[CT][GA]AG SspBI 200 TGTACA T^GTACA StuI 137 AGGCCT AGG^CCT XbaI 331 TCTAGA T^CTAGA XhoII 738 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AccII 471 CGCG CG^CG 1413 CGCG AlwI 738 GGATC GGATC(4/5) 925 GGATC Asp700I 216 GAACTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 310 GAACATCTTC BalI 2 TGGCCA TGG^CCA 35 TGGCCA BfaI 332 CTAG C^TAG 584 CTAG BmyI 100 GTGCCC G[AGT]GC[ACT]^C 549 GGGCAC BsmAI 1216 GAGAC GTCTC(1/5) 934 GTCTC BsmFI 146 GGGAC GGGAC(10/14) 776 GGGAC Bsp1286I 100 GTGCCC G[AGT]GC[ACT]^C 549 GGGCAC BspMI 419 ACCTGC ACCTGC(4/8) 1421 ACCTGC BstUI 471 CGCG CG^CG 1413 CGCG DraII 146 GGGACCT [GA]G^G[ACGT]CC[CT] 404 AGGCCCT DraIII 166 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG 552 CACACAGTG DsaI 1128 CCATGG C^C[GA][CT]GG 1163 CCATGG EcoO109I 146 GGGACCT [GA]G^G[ACGT]CC[CT] 404 AGGCCCT HapII 1354 CCGG C^CGG 1545 CCGG HpaII 1354 CCGG C^CGG 1545 CCGG MaeI 332 CTAG C^TAG 584 CTAG MaeII 235 ACGT A^CGT 1472 ACGT MluNI 2 TGGCCA TGG^CCA 35 TGGCCA MscI 2 TGGCCA TGG^CCA 35 TGGCCA MspA1I 31 CAGCTG C[AC]G^C[GT]G 1363 CCGCTG MspI 1354 CCGG C^CGG 1545 CCGG MvnI 471 CGCG CG^CG 1413 CGCG NcoI 1128 CCATGG C^CATGG 1163 CCATGG SexAI 167 ACCAGGT A^CC[AT]GGT 1447 ACCTGGT SphI 128 GCATGC GCATG^C 423 GCATGC TaqI 496 TCGA T^CGA 1030 TCGA TfiI 498 GATTC G^A[AT]TC 1032 GAATC TthHB8I 496 TCGA T^CGA 1030 TCGA XmnI 216 GAACTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 310 GAACATCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 58 enzymes) # ######################################### AciI AcsI AfaI AluI ApoI AvaII BanI BbvI BclI BpmI BsaJI BsiYI BslI BsrI BssKI BstNI Cac8I CfoI Cfr13I DdeI DpnII EaeI EcoRII EcoT14I FauI FbaI Fnu4HI HaeIII HhaI HinP1I HinfI HphI ItaI MaeIII MboI MboII MnlI MseI MslI MvaI MwoI NdeII NlaIII NlaIV NspI PflMI RsaI Sau3AI Sau96I ScrFI SfaNI StyI Tru9I TseI Tsp45I Tsp509I TspRI Van91I