Restriction map for
pFN21AB5459
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# NO CUT ENZYMES ( 153 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AgeI AhdI
Alw44I AlwNI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvrII
BbeI BbrPI BbvCI BcgI BciVI
BfrI BlnI BlpI BpmI Bpu1102I
BsaAI BsaHI BsaWI BseAI BsiEI
BsiHKAI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BsrBI BsrDI BsrFI
BsrGI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
CelII Cfr10I ClaI CpoI DraI
DraIII DrdI DsaI EagI Eam1105I
EarI EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FseI
HaeII HgaI Hin1I HincII HindII
HindIII HpaI KasI KpnI Ksp632I
KspI MaeII MfeI MluI MroI
MslI MspA1I MunI MvnI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SwaI Tsp45I
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccI 361 GTAGAC GT^[AC][GT]AC
AflIII 610 ACATGT A^C[GA][CT]GT
AocI 316 CCTCAGG CC^T[ACGT]AGG
AvaI 393 CCCGAG C^[CT]CG[GA]G
AvaII 627 GGTCC G^G[AT]CC
AviII 739 TGCGCA TGC^GCA
BanI 471 GGCACC G^G[CT][GA]CC
BanII 1035 GAGCCC G[GA]GC[CT]^C
BclI 266 TGATCA T^GATCA
BcnI 87 CCGGG CC^[GC]GG
BfaI 1054 CTAG C^TAG
BglI 640 GCCATTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 960 AGATCT A^GATCT
BmyI 1035 GAGCCC G[AGT]GC[ACT]^C
BsaI 273 GAGACC GGTCTC(1/5)
BsgI 1168 GTGCAG GTGCAG(16/14)
BsoBI 393 CCCGAG C^[CT]CG[GA]G
Bsp1286I 1035 GAGCCC G[AGT]GC[ACT]^C
BspHI 269 TCATGA T^CATGA
BspLU11I 610 ACATGT A^CATGT
BsrI 368 ACTGG ACTGG(1/-1)
BssSI 116 CTCGTG CACGAG(-5/-1)
Bsu36I 316 CCTCAGG CC^T[ACGT]AGG
DraII 321 GGGGCCT [GA]G^G[ACGT]CC[CT]
Eco81I 316 CCTCAGG CC^T[ACGT]AGG
EcoO109I 321 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 680 CCAAGG C^C[AT][AT]GG
FbaI 266 TGATCA T^GATCA
FspI 739 TGCGCA TGC^GCA
HapII 87 CCGG C^CGG
HpaII 87 CCGG C^CGG
MaeI 1054 CTAG C^TAG
MaeIII 112 GTTAC ^GT[ACGT]AC
MspI 87 CCGG C^CGG
NciI 87 CCGGG CC^[GC]GG
PleI 690 GACTC GAGTC(4/5)
RcaI 269 TCATGA T^CATGA
SspI 818 AATATT AAT^ATT
StuI 781 AGGCCT AGG^CCT
StyI 680 CCAAGG C^C[AT][AT]GG
TfiI 563 GATTC G^A[AT]TC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
BalI 672 TGGCCA TGG^CCA
926 TGGCCA
BbsI 217 GAAGAC GAAGAC(2/6)
235 GAAGAC
BpuAI 217 GAAGAC GAAGAC(2/6)
235 GAAGAC
BsaBI 261 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
582 GATTTCCATC
BseRI 989 CTCCTC GAGGAG(10/8)
1046 CTCCTC
BsmFI 733 GGGAC GGGAC(10/14)
628 GTCCC
BspMI 206 ACCTGC ACCTGC(4/8)
872 ACCTGC
CfoI 210 GCGC GCG^C
740 GCGC
FauI 650 CCCGC CCCGC(4/6)
528 GCGGG
HhaI 210 GCGC GCG^C
740 GCGC
HinP1I 210 GCGC G^CGC
740 GCGC
HinfI 563 GATTC G^A[ACGT]TC
690 GACTC
MamI 261 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
582 GATTTCCATC
MluNI 672 TGGCCA TGG^CCA
926 TGGCCA
MscI 672 TGGCCA TGG^CCA
926 TGGCCA
MseI 41 TTAA T^TAA
466 TTAA
SmlI 407 CTCAAG C^T[CT][GA]AG
1073 CTCAAG
TaqI 546 TCGA T^CGA
810 TCGA
Tru9I 41 TTAA T^TAA
466 TTAA
TspRI 66 CAGTG CA[GC]TG[ACGT][ACGT]^
184 CAGTG
TthHB8I 546 TCGA T^CGA
810 TCGA
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# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AciI AfaI AluI AlwI BamHI
BbvI BsaJI BsiYI BslI BsmAI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII EaeI EcoRII
Fnu4HI FokI HaeIII HphI ItaI
MboI MboII MflI MnlI MvaI
MwoI NdeII NlaIII NlaIV NspI
RsaI Sau3AI Sau96I ScrFI SfaNI
TseI Tsp509I XhoII