(Circular) (Six-base) MAPSORT of: pFN21AB5459.seq Check: 9083 from: 1 to: 5878 pFN21AB5459 5878 bp With 182 enzymes: SgfI * October 20, 2009 10:35 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3688 278 Size: 53 83 186 936 2152 2468 Fragments arranged by size: 2468 2152 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2367 3221 1937 Size: 430 854 4594 Fragments arranged by size: 4594 854 430 Acc65I G'GTAC_C Cuts at: 3199 3199 Size: 5878 AclI AA'CG_TT Cuts at: 1563 4005 4378 1563 Size: 2442 373 3063 Fragments arranged by size: 3063 2442 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3042 3941 5325 5593 1632 Size: 1410 899 1384 268 1917 Fragments arranged by size: 1917 1410 1384 899 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5681 AflIII A'CryG_T Cuts at: 1203 2615 4777 5754 1203 Size: 1412 2162 977 1327 Fragments arranged by size: 2162 1412 1327 977 AgeI A'CCGG_T Cuts at: 4709 4709 Size: 5878 AhdI GACnn_n'nnGTC Cuts at: 4607 4607 Size: 5878 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5878 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5878 AlwNI CAG_nnn'CTG Cuts at: 3621 5193 3621 Size: 1572 4306 ApaLI G'TGCA_C Cuts at: 3935 5091 3935 Size: 1156 4722 ApoI r'AATT_y Cuts at: 1493 3187 3413 3565 5674 1493 Size: 1694 226 152 2109 1697 Fragments arranged by size: 2109 1697 1694 226 152 AseI AT'TA_AT Cuts at: 160 1032 4432 160 Size: 872 3400 1606 Fragments arranged by size: 3400 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5878 AvaI C'yCGr_G Cuts at: 1870 1943 2398 3203 1870 Size: 73 455 805 4545 Fragments arranged by size: 4545 805 455 73 BamHI G'GATC_C Cuts at: 2139 2180 2855 3148 3208 3575 2139 Size: 41 675 293 60 367 4442 Fragments arranged by size: 4442 675 367 293 60 41 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2476 3199 4654 618 Size: 325 217 627 689 723 1455 1842 Fragments arranged by size: 1842 1455 723 689 627 325 217 BanII G_rGCy'C Cuts at: 1391 3044 3197 1391 Size: 1653 153 4072 Fragments arranged by size: 4072 1653 153 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2229 2247 928 Size: 971 330 18 4559 Fragments arranged by size: 4559 971 330 18 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4069 4103 1903 Size: 34 2132 34 3678 Fragments arranged by size: 3678 2132 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3770 4980 3770 Size: 1210 4668 BclI T'GATC_A Cuts at: 1548 2271 1548 Size: 723 5155 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2651 4489 136 Size: 107 122 71 631 605 979 1838 1525 Fragments arranged by size: 1838 1525 979 631 605 122 107 71 BglII A'GATC_T Cuts at: 2965 5873 2965 Size: 2908 2970 BlpI GC'TnA_GC Cuts at: 3295 3295 Size: 5878 Bme1580I G_kGCm'C Cuts at: 1790 3939 5095 1790 Size: 2149 1156 2573 Fragments arranged by size: 2573 2149 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5878 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2383 3609 4567 Size: 416 53 83 399 24 966 1226 958 Cuts at: 4567 442 Size: 1753 Fragments arranged by size: 1753 1226 966 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5878 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4538 1239 Size: 3299 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2397 3063 4003 4868 5166 2397 Size: 666 940 865 298 3109 Fragments arranged by size: 3109 940 865 666 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2272 4541 882 Size: 617 773 2269 2219 Fragments arranged by size: 2269 2219 773 617 BsaAI yAC'GTr Cuts at: 493 1204 3669 493 Size: 711 2465 2702 Fragments arranged by size: 2702 2465 711 BsaBI GATnn'nnATC Cuts at: 2270 2591 5872 2270 Size: 321 3281 2276 Fragments arranged by size: 3281 2276 321 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3685 4067 4702 275 Size: 53 83 186 936 2152 382 635 1451 Fragments arranged by size: 2152 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4311 4709 4983 5130 5541 4311 Size: 398 274 147 411 4648 Fragments arranged by size: 4648 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5653 Fragments arranged by size: 5653 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2985 3042 1186 Size: 1799 57 4022 Fragments arranged by size: 4022 1799 57 BseYI C'CCAG_C Cuts at: 2184 5081 5577 2184 Size: 2897 496 2485 Fragments arranged by size: 2897 2485 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3194 1163 Size: 573 1458 3847 Fragments arranged by size: 3847 1458 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3328 4089 5117 1540 Size: 275 186 1327 761 1028 2301 Fragments arranged by size: 2301 1327 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3939 4024 5095 3939 Size: 85 1071 4722 Fragments arranged by size: 4722 1071 85 BsmI GAATG_Cn' Cuts at: 3389 3482 3389 Size: 93 5785 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3044 3197 3939 4024 5095 1391 Size: 399 1254 153 742 85 1071 2174 Fragments arranged by size: 2174 1254 1071 742 399 153 85 BspEI T'CCGG_A Cuts at: 5541 5541 Size: 5878 BspHI T'CATG_A Cuts at: 2274 3767 5497 2274 Size: 1493 1730 2655 Fragments arranged by size: 2655 1730 1493 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2220 2886 3166 3233 844 Size: 1376 666 280 67 3489 Fragments arranged by size: 3489 1376 666 280 67 BsrBI CCG'CTC Cuts at: 1394 1823 3765 1394 Size: 429 1942 3507 Fragments arranged by size: 3507 1942 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4373 4547 65 Size: 1183 3125 174 1396 Fragments arranged by size: 3125 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4522 4709 1700 Size: 110 2712 187 2869 Fragments arranged by size: 2869 2712 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5878 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5878 BssSI C'ACGA_G Cuts at: 2121 3938 4950 2121 Size: 1817 1012 3049 Fragments arranged by size: 3049 1817 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5878 BstBI TT'CG_AA Cuts at: 4682 4682 Size: 5878 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5878 BstYI r'GATC_y Cuts at: 1972 2139 2180 2443 2855 2965 3148 3208 3575 Size: 167 41 263 412 110 183 60 367 Cuts at: 3575 3960 3977 5429 5515 5527 5873 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 412 385 367 346 263 183 167 110 86 60 41 17 12 Bsu36I CC'TnA_GG Cuts at: 2322 2322 Size: 5878 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4387 Fragments arranged by size: 4387 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5139 Fragments arranged by size: 5139 683 56 BtsI GCAGTG_nn' Cuts at: 2077 2195 3394 4188 4208 2077 Size: 118 1199 794 20 3747 Fragments arranged by size: 3747 1199 794 118 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5878 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5843 DraI TTT'AAA Cuts at: 3182 3182 Size: 5878 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4885 809 Size: 507 3569 1802 Fragments arranged by size: 3569 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2089 2677 2931 Size: 54 1425 50 275 18 259 588 254 Cuts at: 2931 3325 4214 5843 8 Size: 394 889 1629 43 Fragments arranged by size: 1629 1425 889 588 394 275 259 254 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3325 1537 Size: 275 1513 4090 Fragments arranged by size: 4090 1513 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3808 1353 Size: 2455 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4465 4839 4985 387 Size: 1064 138 2876 374 146 1280 Fragments arranged by size: 2876 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3042 3941 4538 5325 5593 1239 Size: 393 1410 899 597 787 268 1524 Fragments arranged by size: 1524 1410 899 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2327 2327 Size: 5878 EcoRI G'AATT_C Cuts at: 3187 3565 3187 Size: 378 5500 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2246 2326 2446 2246 Size: 80 120 5678 Fragments arranged by size: 5678 120 80 FspI TGC'GCA Cuts at: 2746 4384 2746 Size: 1638 4240 FspAI rTGC'GCAy Cuts at: 2746 2746 Size: 5878 HaeII r_GCGC'y Cuts at: 1678 5025 1678 Size: 3347 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4519 4551 4593 4625 1373 Size: 32 135 32 2947 32 42 32 2626 Fragments arranged by size: 2947 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3222 3468 669 Size: 988 281 1284 246 3079 Fragments arranged by size: 3079 1284 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5878 HpaI GTT'AAC Cuts at: 3468 3468 Size: 5878 KpnI G_GTAC'C Cuts at: 3203 3203 Size: 5878 MfeI C'AATT_G Cuts at: 3477 3477 Size: 5878 MluI A'CGCG_T Cuts at: 5754 5754 Size: 5878 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4992 5176 1305 Size: 3687 184 2007 Fragments arranged by size: 3687 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2679 2933 10 Size: 54 1425 343 847 254 2955 Fragments arranged by size: 2955 1425 847 343 254 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 3836 4195 4354 518 Size: 648 843 1827 359 159 2042 Fragments arranged by size: 2042 1827 843 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3294 3971 5119 5364 1606 Size: 1688 677 1148 245 2120 Fragments arranged by size: 2120 1688 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4387 Fragments arranged by size: 4387 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5878 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5878 NotI GC'GGCC_GC Cuts at: 3325 3325 Size: 5878 NspI r_CATG'y Cuts at: 1132 1229 2518 2619 2745 3236 4781 1132 Size: 97 1289 101 126 491 1545 2229 Fragments arranged by size: 2229 1545 1289 491 126 101 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5878 PciI A'CATG_T Cuts at: 2615 4777 2615 Size: 2162 3716 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5878 PfoI T'CCnGG_A Cuts at: 2851 2851 Size: 5878 PmeI GTTT'AAAC Cuts at: 3182 3182 Size: 5878 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2288 2320 3101 3133 3947 3979 5460 5492 2288 Size: 32 781 32 814 32 1481 32 2674 Fragments arranged by size: 2674 1481 814 781 32 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5878 PsiI TTA'TAA Cuts at: 3448 3448 Size: 5878 PstI C_TGCA'G Cuts at: 830 3230 830 Size: 2400 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5878 SalI G'TCGA_C Cuts at: 1936 3220 1936 Size: 1284 4594 SbfI CC_TGCA'GG Cuts at: 3230 3230 Size: 5878 ScaI AGT'ACT Cuts at: 4126 5838 4126 Size: 1712 4166 SfcI C'TryA_G Cuts at: 826 1045 3226 4361 5042 5233 826 Size: 219 2181 1135 681 191 1471 Fragments arranged by size: 2181 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5878 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5878 SmaI CCC'GGG Cuts at: 1872 3205 1872 Size: 1333 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2412 3078 3982 4883 5145 820 Size: 197 926 469 666 904 901 262 1553 Fragments arranged by size: 1553 926 904 901 666 469 262 197 SnaBI TAC'GTA Cuts at: 493 3669 493 Size: 3176 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5878 SphI G_CATG'C Cuts at: 1132 3236 1132 Size: 2104 3774 SspI AAT'ATT Cuts at: 5 52 2825 3802 5 Size: 47 2773 977 2081 Fragments arranged by size: 2773 2081 977 47 StuI AGG'CCT Cuts at: 1517 2788 1517 Size: 1271 4607 StyI C'CwwG_G Cuts at: 513 1065 2004 2685 3317 513 Size: 552 939 681 632 3074 Fragments arranged by size: 3074 939 681 632 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2349 2365 3905 3922 4075 4260 4705 Size: 1255 769 16 1540 17 153 185 445 Cuts at: 4705 325 Size: 1498 Fragments arranged by size: 1540 1498 1255 769 445 185 153 17 16 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2298 4124 5836 Size: 274 80 33 51 1443 321 1826 1712 Cuts at: 5836 96 Size: 138 Fragments arranged by size: 1826 1712 1443 321 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5838 5870 5838 Size: 32 5846 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5878 XbaI T'CTAG_A Cuts at: 3214 3214 Size: 5878 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5878 XmaI C'CCGG_G Cuts at: 1870 3203 1870 Size: 1333 4545 XmnI GAAnn'nnTTC Cuts at: 4007 4007 Size: 5878 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3686 276 Size: 53 83 186 936 2152 2468 Fragments arranged by size: 2468 2152 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI