Restriction map for
pFN21AB5459
(Linear) (Six-base) MAPSORT of: KIBB5459.seq Check: 6710 from: 1 to: 1173
KIBB5459.orf
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AccI GT'mk_AC
Cuts at: 0 362 1173
Size: 362 811
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1037 1173
Size: 1037 136
AflIII A'CryG_T
Cuts at: 0 610 1173
Size: 610 563
AvaI C'yCGr_G
Cuts at: 0 393 1173
Size: 393 780
BamHI G'GATC_C
Cuts at: 0 134 175 850 1143 1173
Size: 134 41 675 293 30
Fragments arranged by size:
675 293 134 41 30
BanI G'GyrC_C
Cuts at: 0 471 1173
Size: 471 702
BanII G_rGCy'C
Cuts at: 0 1039 1173
Size: 1039 134
BbsI GAAGACnn'nnnn_
Cuts at: 0 224 242 1173
Size: 224 18 931
Fragments arranged by size:
931 224 18
BclI T'GATC_A
Cuts at: 0 266 1173
Size: 266 907
BglI GCCn_nnn'nGGC
Cuts at: 0 646 1173
Size: 646 527
BglII A'GATC_T
Cuts at: 0 960 1173
Size: 960 213
BmrI ACTGGGnnnn_n'
Cuts at: 0 378 1173
Size: 378 795
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 392 1058 1173
Size: 392 666 115
Fragments arranged by size:
666 392 115
BsaI GGTCTCn'nnnn_
Cuts at: 0 267 1173
Size: 267 906
BsaBI GATnn'nnATC
Cuts at: 0 265 586 1173
Size: 265 321 587
Fragments arranged by size:
587 321 265
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 980 1037 1173
Size: 980 57 136
Fragments arranged by size:
980 136 57
BseYI C'CCAG_C
Cuts at: 0 179 1173
Size: 179 994
Bsp1286I G_dGCh'C
Cuts at: 0 1039 1173
Size: 1039 134
BspHI T'CATG_A
Cuts at: 0 269 1173
Size: 269 904
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 215 881 1173
Size: 215 666 292
Fragments arranged by size:
666 292 215
BssSI C'ACGA_G
Cuts at: 0 116 1173
Size: 116 1057
BstYI r'GATC_y
Cuts at: 0 134 175 438 850 960 1143 1173
Size: 134 41 263 412 110 183 30
Fragments arranged by size:
412 263 183 134 110 41 30
Bsu36I CC'TnA_GG
Cuts at: 0 317 1173
Size: 317 856
BtsI GCAGTG_nn'
Cuts at: 0 72 190 1173
Size: 72 118 983
Fragments arranged by size:
983 118 72
EaeI y'GGCC_r
Cuts at: 0 84 672 926 1173
Size: 84 588 254 247
Fragments arranged by size:
588 254 247 84
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1037 1173
Size: 1037 136
EcoO109I rG'GnC_Cy
Cuts at: 0 322 1173
Size: 322 851
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 241 321 441 1173
Size: 241 80 120 732
Fragments arranged by size:
732 241 120 80
FspI TGC'GCA
Cuts at: 0 741 1173
Size: 741 432
FspAI rTGC'GCAy
Cuts at: 0 741 1173
Size: 741 432
MscI TGG'CCA
Cuts at: 0 674 928 1173
Size: 674 254 245
Fragments arranged by size:
674 254 245
NspI r_CATG'y
Cuts at: 0 513 614 740 1173
Size: 513 101 126 433
Fragments arranged by size:
513 433 126 101
PciI A'CATG_T
Cuts at: 0 610 1173
Size: 610 563
PfoI T'CCnGG_A
Cuts at: 0 846 1173
Size: 846 327
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 283 315 1096 1128 1173
Size: 283 32 781 32 45
Fragments arranged by size:
781 283 45 32 32
SmlI C'TyrA_G
Cuts at: 0 407 1073 1173
Size: 407 666 100
Fragments arranged by size:
666 407 100
SspI AAT'ATT
Cuts at: 0 820 1173
Size: 820 353
StuI AGG'CCT
Cuts at: 0 783 1173
Size: 783 390
StyI C'CwwG_G
Cuts at: 0 680 1173
Size: 680 493
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 344 360 1173
Size: 344 16 813
Fragments arranged by size:
813 344 16
TatI w'GTAC_w
Cuts at: 0 293 1173
Size: 293 880
Enzymes that do cut:
AccI AcuI AflIII AvaI BamHI BanI BanII BbsI
BclI BglI BglII BmrI BpuEI BsaI BsaBI BseRI
BseYI Bsp1286I BspHI BspMI BssSI BstYI Bsu36I BtsI
EaeI Eco57MI EcoO109I FalI FspI FspAI MscI NspI
PciI PfoI PpiI SmlI SspI StuI StyI TaqII
TatI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BbeI BbvCI BcgI BciVI
BfrBI BlpI Bme1580I BmgBI BmtI BplI BpmI Bpu10I
BsaAI BsaHI BsaWI BsaXI BsgI BsiEI BsiHKAI BsiWI
BsmI BsmBI BspEI BsrBI BsrDI BsrFI BsrGI BssHII
BstAPI BstBI BstEII BstXI BstZ17I BtgI BtgZI ClaI
CspCI DraI DraIII DrdI EagI EarI EciI EcoICRI
EcoNI EcoRI EcoRV FseI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PflMI PmeI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SwaI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI