(Circular) (Six-base) MAPSORT of: pFN21AE0547.seq Check: 6816 from: 1 to: 5974 pFN21AE0547 5974 bp With 182 enzymes: SgfI * October 20, 2009 10:49 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3784 278 Size: 53 83 186 936 2248 2468 Fragments arranged by size: 2468 2248 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3317 1937 Size: 1380 4594 Acc65I G'GTAC_C Cuts at: 3295 3295 Size: 5974 AclI AA'CG_TT Cuts at: 1563 4101 4474 1563 Size: 2538 373 3063 Fragments arranged by size: 3063 2538 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2903 2984 4037 5421 5689 1632 Size: 1271 81 1053 1384 268 1917 Fragments arranged by size: 1917 1384 1271 1053 268 81 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5777 AflIII A'CryG_T Cuts at: 1203 2640 2709 4873 5850 1203 Size: 1437 69 2164 977 1327 Fragments arranged by size: 2164 1437 1327 977 69 AgeI A'CCGG_T Cuts at: 4805 4805 Size: 5974 AhdI GACnn_n'nnGTC Cuts at: 4703 4703 Size: 5974 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5974 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5974 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2696 2728 2696 Size: 32 5942 AlwNI CAG_nnn'CTG Cuts at: 2406 2881 3214 3717 5289 2406 Size: 475 333 503 1572 3091 Fragments arranged by size: 3091 1572 503 475 333 ApaI G_GGCC'C Cuts at: 3222 3222 Size: 5974 ApaLI G'TGCA_C Cuts at: 4031 5187 4031 Size: 1156 4818 ApoI r'AATT_y Cuts at: 1493 2107 3283 3509 3661 5770 1493 Size: 614 1176 226 152 2109 1697 Fragments arranged by size: 2109 1697 1176 614 226 152 AseI AT'TA_AT Cuts at: 160 1032 3048 4528 160 Size: 872 2016 1480 1606 Fragments arranged by size: 2016 1606 1480 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5974 AvaI C'yCGr_G Cuts at: 1870 1943 2487 2686 3244 3299 1870 Size: 73 544 199 558 55 4545 Fragments arranged by size: 4545 558 544 199 73 55 BamHI G'GATC_C Cuts at: 3304 3671 3304 Size: 367 5607 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2893 3295 4750 618 Size: 325 217 627 1106 402 1455 1842 Fragments arranged by size: 1842 1455 1106 627 402 325 217 BanII G_rGCy'C Cuts at: 1391 2396 3222 3293 1391 Size: 1005 826 71 4072 Fragments arranged by size: 4072 1005 826 71 BbeI G_GCGC'C Cuts at: 2897 2897 Size: 5974 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5003 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2830 2864 2969 3003 3216 3250 4165 Size: 34 893 34 105 34 213 34 915 Cuts at: 4165 4199 1903 Size: 34 3678 Fragments arranged by size: 3678 915 893 213 105 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3866 5076 3866 Size: 1210 4764 BclI T'GATC_A Cuts at: 1548 2052 1548 Size: 504 5470 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4585 136 Size: 107 122 71 631 605 2913 1525 Fragments arranged by size: 2913 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5969 5969 Size: 5974 BlpI GC'TnA_GC Cuts at: 3391 3391 Size: 5974 Bme1580I G_kGCm'C Cuts at: 1790 3222 4035 5191 1790 Size: 1432 813 1156 2573 Fragments arranged by size: 2573 1432 1156 813 BmgBI CAC'GTC Cuts at: 1318 3006 1318 Size: 1688 4286 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3705 4663 442 Size: 416 53 83 399 24 2288 958 1753 Fragments arranged by size: 2288 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5974 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3275 3275 Size: 5974 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2288 2831 4634 1239 Size: 1049 543 1803 2579 Fragments arranged by size: 2579 1803 1049 543 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3017 4099 4964 5262 3017 Size: 1082 865 298 3729 Fragments arranged by size: 3729 1082 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4637 882 Size: 617 3138 2219 Fragments arranged by size: 3138 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2539 2619 3765 493 Size: 711 1335 80 1146 2702 Fragments arranged by size: 2702 1335 1146 711 80 BsaBI GATnn'nnATC Cuts at: 5968 5968 Size: 5974 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2894 3781 4163 4798 Size: 53 83 186 936 1361 887 382 635 Cuts at: 4798 275 Size: 1451 Fragments arranged by size: 1451 1361 936 887 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2385 4407 4805 5079 5226 5637 2385 Size: 2022 398 274 147 411 2722 Fragments arranged by size: 2722 2022 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5749 Fragments arranged by size: 5749 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2393 1186 Size: 1207 4767 BseYI C'CCAG_C Cuts at: 5177 5673 5177 Size: 496 5478 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2653 2894 1163 Size: 573 917 241 4243 Fragments arranged by size: 4243 917 573 241 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3236 3424 4185 5213 1540 Size: 275 186 1235 188 761 1028 2301 Fragments arranged by size: 2301 1235 1028 761 275 188 186 BsiHKAI G_wGCw'C Cuts at: 2819 4035 4120 5191 2819 Size: 1216 85 1071 3602 Fragments arranged by size: 3602 1216 1071 85 BsmI GAATG_Cn' Cuts at: 2933 2992 3485 3578 2933 Size: 59 493 93 5329 Fragments arranged by size: 5329 493 93 59 BsmBI CGTCTCn'nnnn_ Cuts at: 3003 3003 Size: 5974 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2396 2819 3222 3293 4035 4120 5191 Size: 399 606 423 403 71 742 85 1071 Cuts at: 5191 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 606 423 403 399 85 71 BspEI T'CCGG_A Cuts at: 2385 5637 2385 Size: 3252 2722 BspHI T'CATG_A Cuts at: 2412 3863 5593 2412 Size: 1451 1730 2793 Fragments arranged by size: 2793 1730 1451 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2601 2805 3329 844 Size: 1757 204 524 3489 Fragments arranged by size: 3489 1757 524 204 BsrBI CCG'CTC Cuts at: 1394 1823 3254 3861 1394 Size: 429 1431 607 3507 Fragments arranged by size: 3507 1431 607 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4469 4643 65 Size: 1183 3221 174 1396 Fragments arranged by size: 3221 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2661 2896 4618 4805 1700 Size: 110 851 235 1722 187 2869 Fragments arranged by size: 2869 1722 851 235 187 110 BsrGI T'GTAC_A Cuts at: 96 2643 96 Size: 2547 3427 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5974 BssSI C'ACGA_G Cuts at: 4034 5046 4034 Size: 1012 4962 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5974 BstBI TT'CG_AA Cuts at: 4778 4778 Size: 5974 BstEII G'GTnAC_C Cuts at: 1184 2833 1184 Size: 1649 4325 BstXI CCAn_nnnn'nTGG Cuts at: 2701 2771 2701 Size: 70 5904 BstYI r'GATC_y Cuts at: 1972 2856 3304 3671 4056 4073 5525 5611 5623 Size: 884 448 367 385 17 1452 86 12 Cuts at: 5623 5969 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 884 448 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3117 3117 Size: 5974 BtgI C'CryG_G Cuts at: 513 1065 2004 2770 2821 513 Size: 552 939 766 51 3666 Fragments arranged by size: 3666 939 766 552 51 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3049 487 Size: 683 56 1823 3412 Fragments arranged by size: 3412 1823 683 56 BtsI GCAGTG_nn' Cuts at: 2627 2917 3012 3490 4284 4304 2627 Size: 290 95 478 794 20 4297 Fragments arranged by size: 4297 794 478 290 95 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5974 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5939 DraI TTT'AAA Cuts at: 3278 3278 Size: 5974 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2186 4981 809 Size: 507 870 2795 1802 Fragments arranged by size: 2795 1802 870 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3421 4310 5939 Size: 54 1425 50 275 18 1591 889 1629 Cuts at: 5939 8 Size: 43 Fragments arranged by size: 1629 1591 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3421 1537 Size: 275 1609 4090 Fragments arranged by size: 4090 1609 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2384 3904 1353 Size: 1031 1520 3423 Fragments arranged by size: 3423 1520 1031 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4561 4935 5081 387 Size: 1064 138 2972 374 146 1280 Fragments arranged by size: 2972 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2288 2831 2903 2984 4037 4634 5421 Size: 393 656 543 72 81 1053 597 787 Cuts at: 5421 5689 1239 Size: 268 1524 Fragments arranged by size: 1524 1053 787 656 597 543 393 268 81 72 EcoO109I rG'GnC_Cy Cuts at: 2227 3218 2227 Size: 991 4983 EcoRI G'AATT_C Cuts at: 3283 3661 3283 Size: 378 5596 EcoRV GAT'ATC Cuts at: 2035 2165 2035 Size: 130 5844 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2464 2938 2464 Size: 474 5500 FspI TGC'GCA Cuts at: 4480 4480 Size: 5974 HaeII r_GCGC'y Cuts at: 1678 2897 5121 1678 Size: 1219 2224 2531 Fragments arranged by size: 2531 2224 1219 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2064 2096 2154 2186 3184 Size: 32 135 32 492 32 58 32 998 Cuts at: 3184 3216 4615 4647 4689 4721 1373 Size: 32 1399 32 42 32 2626 Fragments arranged by size: 2626 1399 998 492 135 58 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2191 3318 3564 669 Size: 988 281 253 1127 246 3079 Fragments arranged by size: 3079 1127 988 281 253 246 HindIII A'AGCT_T Cuts at: 748 2648 748 Size: 1900 4074 HpaI GTT'AAC Cuts at: 3564 3564 Size: 5974 KasI G'GCGC_C Cuts at: 2893 2893 Size: 5974 KpnI G_GTAC'C Cuts at: 3299 3299 Size: 5974 MfeI C'AATT_G Cuts at: 3573 3573 Size: 5974 MluI A'CGCG_T Cuts at: 5850 5850 Size: 5974 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5088 5272 1305 Size: 3783 184 2007 Fragments arranged by size: 3783 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4152 Fragments arranged by size: 4152 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2345 2769 3932 4291 4450 518 Size: 648 1179 424 1163 359 159 2042 Fragments arranged by size: 2042 1179 1163 648 424 359 159 MspA1I CmG'CkG Cuts at: 1606 2357 2823 3390 4067 5215 5460 1606 Size: 751 466 567 677 1148 245 2120 Fragments arranged by size: 2120 1148 751 677 567 466 245 NarI GG'CG_CC Cuts at: 2894 2894 Size: 5974 NcoI C'CATG_G Cuts at: 513 1065 2004 2770 513 Size: 552 939 766 3717 Fragments arranged by size: 3717 939 766 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5974 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5974 NotI GC'GGCC_GC Cuts at: 3421 3421 Size: 5974 NspI r_CATG'y Cuts at: 1132 1229 2644 2713 3332 4877 1132 Size: 97 1415 69 619 1545 2229 Fragments arranged by size: 2229 1545 1415 619 97 69 PasI CC'CwG_GG Cuts at: 1348 2434 3166 1348 Size: 1086 732 4156 Fragments arranged by size: 4156 1086 732 PciI A'CATG_T Cuts at: 2640 2709 4873 2640 Size: 69 2164 3741 Fragments arranged by size: 3741 2164 69 PflMI CCAn_nnn'nTGG Cuts at: 1108 2406 1108 Size: 1298 4676 PmeI GTTT'AAAC Cuts at: 3278 3278 Size: 5974 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4043 4075 5556 5588 4043 Size: 32 1481 32 4429 Fragments arranged by size: 4429 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2227 2227 Size: 5974 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5974 PsiI TTA'TAA Cuts at: 3544 3544 Size: 5974 PspOMI G'GGCC_C Cuts at: 3218 3218 Size: 5974 PstI C_TGCA'G Cuts at: 830 3326 830 Size: 2496 3478 PvuI CG_AT'CG Cuts at: 2001 3236 2001 Size: 1235 4739 SacII CC_GC'GG Cuts at: 2824 2824 Size: 5974 SalI G'TCGA_C Cuts at: 1936 3316 1936 Size: 1380 4594 SapI GCTCTTCn'nnn_ Cuts at: 2384 2384 Size: 5974 SbfI CC_TGCA'GG Cuts at: 3326 3326 Size: 5974 ScaI AGT'ACT Cuts at: 4222 5934 4222 Size: 1712 4262 SfcI C'TryA_G Cuts at: 826 1045 3322 4457 5138 5329 826 Size: 219 2277 1135 681 191 1471 Fragments arranged by size: 2277 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2895 2895 Size: 5974 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5974 SgrAI Cr'CCGG_yG Cuts at: 1700 2896 1700 Size: 1196 4778 SmaI CCC'GGG Cuts at: 1872 2489 3301 1872 Size: 617 812 4545 Fragments arranged by size: 4545 812 617 SmlI C'TyrA_G Cuts at: 820 1017 1943 3032 4078 4979 5241 820 Size: 197 926 1089 1046 901 262 1553 Fragments arranged by size: 1553 1089 1046 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3765 493 Size: 3272 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5974 SphI G_CATG'C Cuts at: 1132 3332 1132 Size: 2200 3774 SspI AAT'ATT Cuts at: 5 52 3898 5 Size: 47 3846 2081 Fragments arranged by size: 3846 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5974 StyI C'CwwG_G Cuts at: 513 1065 2004 2770 3413 513 Size: 552 939 766 643 3074 Fragments arranged by size: 3074 939 766 643 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3039 4001 4018 4171 4356 4801 325 Size: 1255 1459 962 17 153 185 445 1498 Fragments arranged by size: 1498 1459 1255 962 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2643 3036 4220 Size: 274 80 33 51 1443 666 393 1184 Cuts at: 4220 5932 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1184 666 393 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2608 2640 5934 5966 2608 Size: 32 3294 32 2616 Fragments arranged by size: 3294 2616 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5974 XbaI T'CTAG_A Cuts at: 3310 3310 Size: 5974 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3096 3096 Size: 5974 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5974 XmaI C'CCGG_G Cuts at: 1870 2487 3299 1870 Size: 617 812 4545 Fragments arranged by size: 4545 812 617 XmnI GAAnn'nnTTC Cuts at: 4103 4103 Size: 5974 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3782 276 Size: 53 83 186 936 2248 2468 Fragments arranged by size: 2468 2248 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BstZ17I DraIII EcoICRI EcoNI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI PvuII RsrII SacI SanDI SexAI SfiI SrfI SwaI