Restriction map for
pFN21AB7352
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# NO CUT ENZYMES ( 112 enzymes) #
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AatII AccIII AclI AcyI AflIII
AgeI AhdI Alw44I ApaLI AscI
AseI AsnI Asp700I AspEI AspI
AviII AvrII BbeI BbsI BbvCI
BcgI BlnI BpuAI BsaBI BsaHI
BsaI BsaWI BseAI BsiEI BsiWI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrFI BsrGI BssHII Bst1107I
BstBI BstEII BstPI BstZ17I Cfr10I
ClaI CpoI DraI DraIII DrdI
DsaI EagI Eam1105I EclXI Eco52I
EcoO65I EcoRV EcoT22I FseI FspI
Hin1I HincII HindII HindIII HpaI
KasI KspI MamI MfeI MluI
MroI MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmeI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI Sse8387I
SspBI SwaI Tth111I Van91I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 30 enzymes) #
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Acc65I 1199 GGTACC G^GTACC
AccI 934 GTCTAC GT^[AC][GT]AC
AflII 271 CTTAAG C^TTAAG
AocI 183 CCTGAGG CC^T[ACGT]AGG
Aor51HI 44 AGCGCT AGC^GCT
Asp718I 1199 GGTACC G^GTACC
BanI 1199 GGTACC G^G[CT][GA]CC
BbrPI 2287 CACGTG CAC^GTG
BclI 302 TGATCA T^GATCA
BfrI 271 CTTAAG C^TTAAG
BglI 402 GCCCTACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BssSI 2392 CACGAG CACGAG(-5/-1)
BstAPI 1821 GCACATGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 785 CCAGGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 183 CCTGAGG CC^T[ACGT]AGG
Eco47III 44 AGCGCT AGC^GCT
Eco81I 183 CCTGAGG CC^T[ACGT]AGG
EcoNI 183 CCTGAGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 2471 CCTTGG C^C[AT][AT]GG
FbaI 302 TGATCA T^GATCA
KpnI 1199 GGTACC GGTAC^C
PmaCI 2287 CACGTG CAC^GTG
PmlI 2287 CACGTG CAC^GTG
SmaI 362 CCCGGG CCC^GGG
SphI 2297 GCATGC GCATG^C
SspI 589 AATATT AAT^ATT
StuI 2264 AGGCCT AGG^CCT
StyI 2471 CCTTGG C^C[AT][AT]GG
XmaCI 362 CCCGGG C^CCGGG
XmaI 362 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 23 enzymes) #
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ApaI 1517 GGGCCC GGGCC^C
2058 GGGCCC
BalI 308 TGGCCA TGG^CCA
1013 TGGCCA
BamHI 74 GGATCC G^GATCC
849 GGATCC
BciVI 1040 GGATAC GTATCC(6/5)
1268 GTATCC
BglII 800 AGATCT A^GATCT
2090 AGATCT
BlpI 1032 GCTGAGC GC^T[ACGT]AGC
2218 GCTGAGC
Bpu1102I 1032 GCTGAGC GC^T[ACGT]AGC
2218 GCTGAGC
BsaAI 1434 CACGTA [CT]AC^GT[GA]
2287 CACGTG
BsmBI 2030 CGTCTC CGTCTC(1/5)
31 GAGACG
BspMI 767 ACCTGC ACCTGC(4/8)
2373 GCAGGT
BsrBI 51 CCGCTC CCGCTC(-3/-3)
70 GAGCGG
BsrDI 1110 CATTGC GCAATG(2/0)
1905 CATTGC
CelII 1032 GCTGAGC GC^T[ACGT]AGC
2218 GCTGAGC
EcoRI 280 GAATTC G^AATTC
1186 GAATTC
HaeII 44 AGCGCT [GA]GCGC^[CT]
1497 GGCGCT
MluNI 308 TGGCCA TGG^CCA
1013 TGGCCA
MscI 308 TGGCCA TGG^CCA
1013 TGGCCA
MseI 272 TTAA T^TAA
1925 TTAA
PpuMI 1481 AGGTCCT [GA]G^G[AT]CC[CT]
2417 AGGTCCT
PshAI 225 GACTGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
390 GACAGCTGTC
SacI 82 GAGCTC GAGCT^C
2221 GAGCTC
SapI 2292 GAAGAGC GCTCTTC(1/4)
1621 GCTCTTC
Tru9I 272 TTAA T^TAA
1925 TTAA
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# 3 OR MORE CUT ENZYMES ( 93 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI AspHI AvaI
AvaII BanII BbvI BcnI BfaI
BmyI BpmI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII EaeI
EarI EcoO109I EcoRII FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI TaqI
TfiI TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII