(Circular) (Six-base) MAPSORT of: pFN21AB7352.seq Check: 9052 from: 1 to: 7234 pFN21AB7352 7234 bp With 182 enzymes: SgfI * April 15, 2010 09:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4369 4369 Size: 7234 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5044 278 Size: 53 83 186 936 3508 2468 Fragments arranged by size: 3508 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2940 4577 1937 Size: 1003 1637 4594 Fragments arranged by size: 4594 1637 1003 Acc65I G'GTAC_C Cuts at: 3204 4555 3204 Size: 1351 5883 AclI AA'CG_TT Cuts at: 1563 5361 5734 1563 Size: 3798 373 3063 Fragments arranged by size: 3798 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2195 2654 3302 3614 3937 4134 5297 6681 Size: 563 459 648 312 323 197 1163 1384 Cuts at: 6681 6949 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 1163 648 563 459 323 312 268 197 AfeI AGC'GCT Cuts at: 2051 2051 Size: 7234 AflII C'TTAA_G Cuts at: 820 1017 2276 820 Size: 197 1259 5778 Fragments arranged by size: 5778 1259 197 AflIII A'CryG_T Cuts at: 1203 6133 7110 1203 Size: 4930 977 1327 Fragments arranged by size: 4930 1327 977 AgeI A'CCGG_T Cuts at: 6065 6065 Size: 7234 AhdI GACnn_n'nnGTC Cuts at: 5963 5963 Size: 7234 AleI CACnn'nnGTG Cuts at: 1166 3060 4292 1166 Size: 1894 1232 4108 Fragments arranged by size: 4108 1894 1232 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3684 4065 1184 Size: 2500 381 4353 Fragments arranged by size: 4353 2500 381 AlwNI CAG_nnn'CTG Cuts at: 2173 4018 4312 4977 6549 2173 Size: 1845 294 665 1572 2858 Fragments arranged by size: 2858 1845 1572 665 294 ApaI G_GGCC'C Cuts at: 3526 4067 3526 Size: 541 6693 ApaLI G'TGCA_C Cuts at: 5291 6447 5291 Size: 1156 6078 ApoI r'AATT_y Cuts at: 1493 2285 3191 3748 4543 4769 4921 7030 1493 Size: 792 906 557 795 226 152 2109 1697 Fragments arranged by size: 2109 1697 906 795 792 557 226 152 AseI AT'TA_AT Cuts at: 160 1032 5788 160 Size: 872 4756 1606 Fragments arranged by size: 4756 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7234 AvaI C'yCGr_G Cuts at: 1870 1943 2147 2208 2367 3962 4559 1870 Size: 73 204 61 159 1595 597 4545 Fragments arranged by size: 4545 1595 597 204 159 73 61 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2543 2576 2543 Size: 33 7201 BamHI G'GATC_C Cuts at: 2079 2854 4564 4931 2079 Size: 775 1710 367 4382 Fragments arranged by size: 4382 1710 775 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3204 4555 6010 618 Size: 325 217 627 1417 1351 1455 1842 Fragments arranged by size: 1842 1455 1417 1351 627 325 217 BanII G_rGCy'C Cuts at: 1391 2091 2680 2935 3335 3438 3526 4067 4230 Size: 700 589 255 400 103 88 541 163 Cuts at: 4230 4553 1391 Size: 323 4072 Fragments arranged by size: 4072 700 589 541 400 323 255 163 103 88 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 6263 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5425 5459 1903 Size: 34 3488 34 3678 Fragments arranged by size: 3678 3488 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3039 3284 5126 6336 3039 Size: 245 1842 1210 3937 Fragments arranged by size: 3937 1842 1210 245 BclI T'GATC_A Cuts at: 1548 2307 1548 Size: 759 6475 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2413 5845 136 Size: 107 122 71 631 605 741 3432 1525 Fragments arranged by size: 3432 1525 741 631 605 122 107 71 BglII A'GATC_T Cuts at: 2805 4095 7229 2805 Size: 1290 3134 2810 Fragments arranged by size: 3134 2810 1290 BlpI GC'TnA_GC Cuts at: 3038 4224 4651 3038 Size: 1186 427 5621 Fragments arranged by size: 5621 1186 427 Bme1580I G_kGCm'C Cuts at: 1790 3526 4054 4067 4509 5295 6451 1790 Size: 1736 528 13 442 786 1156 2573 Fragments arranged by size: 2573 1736 1156 786 528 442 13 BmgBI CAC'GTC Cuts at: 1318 3884 1318 Size: 2566 4668 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3864 4965 5923 Size: 416 53 83 399 24 2447 1101 958 Cuts at: 5923 442 Size: 1753 Fragments arranged by size: 2447 1753 1101 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7234 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2756 3988 2756 Size: 1232 6002 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2054 2183 2870 3571 4505 5894 1239 Size: 815 129 687 701 934 1389 2579 Fragments arranged by size: 2579 1389 934 815 701 687 129 Bpu10I CC'TnA_GC Cuts at: 2085 2613 4056 4273 4434 2085 Size: 528 1443 217 161 4885 Fragments arranged by size: 4885 1443 528 217 161 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2952 3985 5359 6224 6522 2952 Size: 1033 1374 865 298 3664 Fragments arranged by size: 3664 1374 1033 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5897 882 Size: 617 4398 2219 Fragments arranged by size: 4398 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3441 4294 5025 493 Size: 711 2237 853 731 2702 Fragments arranged by size: 2702 2237 853 731 711 BsaBI GATnn'nnATC Cuts at: 7228 7228 Size: 7234 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5041 5423 6058 275 Size: 53 83 186 936 3508 382 635 1451 Fragments arranged by size: 3508 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5667 6065 6339 6486 6897 5667 Size: 398 274 147 411 6004 Fragments arranged by size: 6004 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2920 2950 1178 Size: 30 165 30 1517 30 5462 Fragments arranged by size: 5462 1517 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2135 2730 3299 1186 Size: 949 595 569 5121 Fragments arranged by size: 5121 949 595 569 BseYI C'CCAG_C Cuts at: 2223 4414 6437 6933 2223 Size: 2191 2023 496 2524 Fragments arranged by size: 2524 2191 2023 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3323 3644 4055 4397 1163 Size: 573 1587 321 411 342 4000 Fragments arranged by size: 4000 1587 573 411 342 321 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4684 5445 6473 1540 Size: 275 186 2683 761 1028 2301 Fragments arranged by size: 2683 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2091 4230 4533 5295 5380 6451 2091 Size: 2139 303 762 85 1071 2874 Fragments arranged by size: 2874 2139 1071 762 303 85 BsmI GAATG_Cn' Cuts at: 4745 4838 4745 Size: 93 7141 BsmBI CGTCTCn'nnnn_ Cuts at: 2030 4041 2030 Size: 2011 5223 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2091 2680 2935 3335 3438 3526 4054 Size: 399 301 589 255 400 103 88 528 Cuts at: 4054 4067 4230 4509 4533 4553 5295 5380 6451 Size: 13 163 279 24 20 742 85 1071 Cuts at: 6451 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 589 528 400 399 301 279 255 163 103 88 85 24 20 13 BspEI T'CCGG_A Cuts at: 6897 6897 Size: 7234 BspHI T'CATG_A Cuts at: 5123 6853 5123 Size: 1730 5504 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2781 4369 4589 844 Size: 1937 1588 220 3489 Fragments arranged by size: 3489 1937 1588 220 BsrBI CCG'CTC Cuts at: 1394 1823 2058 2077 5121 1394 Size: 429 235 19 3044 3507 Fragments arranged by size: 3507 3044 429 235 19 BsrDI GCAATG_nn' Cuts at: 65 1248 3114 3909 5729 5903 65 Size: 1183 1866 795 1820 174 1396 Fragments arranged by size: 1866 1820 1396 1183 795 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5878 6065 1700 Size: 110 4068 187 2869 Fragments arranged by size: 4068 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7234 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7234 BssSI C'ACGA_G Cuts at: 4397 5294 6306 4397 Size: 897 1012 5325 Fragments arranged by size: 5325 1012 897 BstAPI GCAn_nnn'nTGC Cuts at: 794 3832 794 Size: 3038 4196 BstBI TT'CG_AA Cuts at: 6038 6038 Size: 7234 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7234 BstXI CCAn_nnnn'nTGG Cuts at: 2797 2797 Size: 7234 BstYI r'GATC_y Cuts at: 1972 2079 2805 2854 3594 4095 4359 4564 4931 Size: 107 726 49 740 501 264 205 367 Cuts at: 4931 5316 5333 6785 6871 6883 7229 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 740 726 501 385 367 346 264 205 107 86 49 17 12 Bsu36I CC'TnA_GG Cuts at: 2189 2189 Size: 7234 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 5743 Fragments arranged by size: 5743 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2101 2533 487 Size: 683 56 875 432 5188 Fragments arranged by size: 5188 875 683 432 56 BtsI GCAGTG_nn' Cuts at: 3809 4054 4750 5544 5564 3809 Size: 245 696 794 20 5479 Fragments arranged by size: 5479 794 696 245 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7234 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7199 DraI TTT'AAA Cuts at: 4538 4538 Size: 7234 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6241 809 Size: 507 4925 1802 Fragments arranged by size: 4925 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2106 2313 3018 Size: 54 1425 50 275 18 276 207 705 Cuts at: 3018 4213 4681 5570 7199 8 Size: 1195 468 889 1629 43 Fragments arranged by size: 1629 1425 1195 889 705 468 276 275 207 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4681 1537 Size: 275 2869 4090 Fragments arranged by size: 4090 2869 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3124 3345 3633 4184 4260 4292 5164 1353 Size: 1771 221 288 551 76 32 872 3423 Fragments arranged by size: 3423 1771 872 551 288 221 76 32 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3266 3608 4367 5821 6195 6341 Size: 1064 138 1677 342 759 1454 374 146 Cuts at: 6341 387 Size: 1280 Fragments arranged by size: 1677 1454 1280 1064 759 374 342 146 138 EcoICRI GAG'CTC Cuts at: 2089 4228 2089 Size: 2139 5095 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2054 2183 2195 2654 2870 3302 3571 Size: 393 422 129 12 459 216 432 269 Cuts at: 3571 3614 3937 4134 4505 5297 5894 6681 6949 Size: 43 323 197 371 792 597 787 268 Cuts at: 6949 1239 Size: 1524 Fragments arranged by size: 1524 792 787 597 459 432 422 393 371 323 269 268 216 197 129 43 12 EcoNI CCTnn'n_nnAGG Cuts at: 2192 2192 Size: 7234 EcoO109I rG'GnC_Cy Cuts at: 2213 3487 3522 4063 4423 2213 Size: 1274 35 541 360 5024 Fragments arranged by size: 5024 1274 541 360 35 EcoRI G'AATT_C Cuts at: 2285 3191 4543 4921 2285 Size: 906 1352 378 4598 Fragments arranged by size: 4598 1352 906 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3199 3199 Size: 7234 FspI TGC'GCA Cuts at: 5740 5740 Size: 7234 HaeII r_GCGC'y Cuts at: 1678 2053 3506 6381 1678 Size: 375 1453 2875 2531 Fragments arranged by size: 2875 2531 1453 375 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2667 2699 2922 2954 3134 Size: 32 135 32 1095 32 223 32 180 Cuts at: 3134 3166 3383 3415 3638 3670 5875 5907 5949 Size: 32 217 32 223 32 2205 32 42 Cuts at: 5949 5981 1373 Size: 32 2626 Fragments arranged by size: 2626 2205 1095 223 223 217 180 135 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4578 4824 669 Size: 988 281 2640 246 3079 Fragments arranged by size: 3079 2640 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7234 HpaI GTT'AAC Cuts at: 4824 4824 Size: 7234 KpnI G_GTAC'C Cuts at: 3208 4559 3208 Size: 1351 5883 MfeI C'AATT_G Cuts at: 4833 4833 Size: 7234 MluI A'CGCG_T Cuts at: 7110 7110 Size: 7234 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2701 3327 4002 6348 6532 1305 Size: 1396 626 675 2346 184 2007 Fragments arranged by size: 2346 2007 1396 675 626 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2315 3020 10 Size: 54 1425 343 483 705 4224 Fragments arranged by size: 4224 1425 705 483 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3060 3699 3722 4292 5192 5551 5710 Size: 648 1894 639 23 570 900 359 159 Cuts at: 5710 518 Size: 2042 Fragments arranged by size: 2042 1894 900 648 639 570 359 159 23 MspA1I CmG'CkG Cuts at: 1606 2101 2227 2399 2797 4069 4112 4650 5327 Size: 495 126 172 398 1272 43 538 677 Cuts at: 5327 6475 6720 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1272 1148 677 538 495 398 245 172 126 43 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5743 Fragments arranged by size: 5743 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7234 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7234 NotI GC'GGCC_GC Cuts at: 4681 4681 Size: 7234 NspI r_CATG'y Cuts at: 1132 1229 3832 4210 4306 4592 6137 1132 Size: 97 2603 378 96 286 1545 2229 Fragments arranged by size: 2603 2229 1545 378 286 97 96 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7234 PciI A'CATG_T Cuts at: 6133 6133 Size: 7234 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7234 PfoI T'CCnGG_A Cuts at: 4365 4365 Size: 7234 PmeI GTTT'AAAC Cuts at: 4538 4538 Size: 7234 PmlI CAC'GTG Cuts at: 4294 4294 Size: 7234 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5303 5335 6816 6848 5303 Size: 32 1481 32 5689 Fragments arranged by size: 5689 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3487 4423 3487 Size: 936 6298 PshAI GACnn'nnGTC Cuts at: 1613 2234 2399 1613 Size: 621 165 6448 Fragments arranged by size: 6448 621 165 PsiI TTA'TAA Cuts at: 2343 4804 2343 Size: 2461 4773 PspOMI G'GGCC_C Cuts at: 3522 4063 3522 Size: 541 6693 PstI C_TGCA'G Cuts at: 830 3084 3453 3576 3837 4044 4144 4586 830 Size: 2254 369 123 261 207 100 442 3478 Fragments arranged by size: 3478 2254 442 369 261 207 123 100 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7234 PvuII CAG'CTG Cuts at: 2101 2227 2399 2797 4112 2101 Size: 126 172 398 1315 5223 Fragments arranged by size: 5223 1315 398 172 126 SacI G_AGCT'C Cuts at: 2091 4230 2091 Size: 2139 5095 SalI G'TCGA_C Cuts at: 1936 4576 1936 Size: 2640 4594 SapI GCTCTTCn'nnn_ Cuts at: 3633 4292 3633 Size: 659 6575 SbfI CC_TGCA'GG Cuts at: 4586 4586 Size: 7234 ScaI AGT'ACT Cuts at: 5482 7194 5482 Size: 1712 5522 SfcI C'TryA_G Cuts at: 826 1045 2410 3080 3449 3491 3572 3833 4040 Size: 219 1365 670 369 42 81 261 207 Cuts at: 4040 4140 4342 4582 5717 6398 6589 826 Size: 100 202 240 1135 681 191 1471 Fragments arranged by size: 1471 1365 1135 681 670 369 261 240 219 207 202 191 100 81 42 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7234 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7234 SmaI CCC'GGG Cuts at: 1872 2369 4561 1872 Size: 497 2192 4545 Fragments arranged by size: 4545 2192 497 SmlI C'TyrA_G Cuts at: 820 1017 1943 2276 2967 4000 5338 6239 6501 Size: 197 926 333 691 1033 1338 901 262 Cuts at: 6501 820 Size: 1553 Fragments arranged by size: 1553 1338 1033 926 901 691 333 262 197 SnaBI TAC'GTA Cuts at: 493 5025 493 Size: 4532 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7234 SphI G_CATG'C Cuts at: 1132 4306 4592 1132 Size: 3174 286 3774 Fragments arranged by size: 3774 3174 286 SspI AAT'ATT Cuts at: 5 52 2596 5158 5 Size: 47 2544 2562 2081 Fragments arranged by size: 2562 2544 2081 47 StuI AGG'CCT Cuts at: 1517 4271 1517 Size: 2754 4480 StyI C'CwwG_G Cuts at: 513 1065 2004 4476 4673 513 Size: 552 939 2472 197 3074 Fragments arranged by size: 3074 2472 939 552 197 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2280 3553 4304 5261 5278 5431 5616 Size: 1255 700 1273 751 957 17 153 185 Cuts at: 5616 6061 325 Size: 445 1498 Fragments arranged by size: 1498 1273 1255 957 751 700 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5480 7192 96 Size: 274 80 33 51 1443 3503 1712 138 Fragments arranged by size: 3503 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2846 2878 2920 2952 7194 7226 2846 Size: 32 42 32 4242 32 2854 Fragments arranged by size: 4242 2854 42 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7234 XbaI T'CTAG_A Cuts at: 4570 4570 Size: 7234 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2128 2308 3325 2128 Size: 180 1017 6037 Fragments arranged by size: 6037 1017 180 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7234 XmaI C'CCGG_G Cuts at: 1870 2367 4559 1870 Size: 497 2192 4545 Fragments arranged by size: 4545 2192 497 XmnI GAAnn'nnTTC Cuts at: 5363 5363 Size: 7234 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5042 276 Size: 53 83 186 936 3508 2468 Fragments arranged by size: 3508 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BbeI BbvCI BfrBI BsiWI BstZ17I DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI