Restriction map for
pFN21AB7352
(Linear) (Six-base) MAPSORT of: KIBB7352.seq Check: 9220 from: 1 to: 2529
KIBB7352.orf
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2364 2529
Size: 2364 165
AccI GT'mk_AC
Cuts at: 0 935 2529
Size: 935 1594
Acc65I G'GTAC_C
Cuts at: 0 1199 2529
Size: 1199 1330
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 190 649 1297 1609 1932 2129 2529
Size: 190 459 648 312 323 197 400
Fragments arranged by size:
648 459 400 323 312 197 190
AfeI AGC'GCT
Cuts at: 0 46 2529
Size: 46 2483
AflII C'TTAA_G
Cuts at: 0 271 2529
Size: 271 2258
AleI CACnn'nnGTG
Cuts at: 0 1055 2287 2529
Size: 1055 1232 242
Fragments arranged by size:
1232 1055 242
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1679 2060 2529
Size: 1679 381 469
Fragments arranged by size:
1679 469 381
AlwNI CAG_nnn'CTG
Cuts at: 0 168 2013 2307 2529
Size: 168 1845 294 222
Fragments arranged by size:
1845 294 222 168
ApaI G_GGCC'C
Cuts at: 0 1521 2062 2529
Size: 1521 541 467
Fragments arranged by size:
1521 541 467
ApoI r'AATT_y
Cuts at: 0 280 1186 1743 2529
Size: 280 906 557 786
Fragments arranged by size:
906 786 557 280
AvaI C'yCGr_G
Cuts at: 0 142 203 362 1957 2529
Size: 142 61 159 1595 572
Fragments arranged by size:
1595 572 159 142 61
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 538 571 2529
Size: 538 33 1958
Fragments arranged by size:
1958 538 33
BamHI G'GATC_C
Cuts at: 0 74 849 2529
Size: 74 775 1680
Fragments arranged by size:
1680 775 74
BanI G'GyrC_C
Cuts at: 0 1199 2529
Size: 1199 1330
BanII G_rGCy'C
Cuts at: 0 86 675 930 1330 1433 1521 2062 2225
Size: 86 589 255 400 103 88 541 163
Cuts at: 2225 2529
Size: 304
Fragments arranged by size:
589 541 400 304 255 163 103 88
86
BciVI GTATCCnnnnn_n'
Cuts at: 0 1034 1279 2529
Size: 1034 245 1250
Fragments arranged by size:
1250 1034 245
BclI T'GATC_A
Cuts at: 0 302 2529
Size: 302 2227
BglI GCCn_nnn'nGGC
Cuts at: 0 408 2529
Size: 408 2121
BglII A'GATC_T
Cuts at: 0 800 2090 2529
Size: 800 1290 439
Fragments arranged by size:
1290 800 439
BlpI GC'TnA_GC
Cuts at: 0 1033 2219 2529
Size: 1033 1186 310
Fragments arranged by size:
1186 1033 310
Bme1580I G_kGCm'C
Cuts at: 0 1521 2049 2062 2504 2529
Size: 1521 528 13 442 25
Fragments arranged by size:
1521 528 442 25 13
BmgBI CAC'GTC
Cuts at: 0 1879 2529
Size: 1879 650
BmrI ACTGGGnnnn_n'
Cuts at: 0 1859 2529
Size: 1859 670
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 751 1983 2529
Size: 751 1232 546
Fragments arranged by size:
1232 751 546
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 178 865 1566 2500 2529
Size: 49 129 687 701 934 29
Fragments arranged by size:
934 701 687 129 49 29
Bpu10I CC'TnA_GC
Cuts at: 0 80 608 2051 2268 2429 2529
Size: 80 528 1443 217 161 100
Fragments arranged by size:
1443 528 217 161 100 80
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 947 1980 2529
Size: 947 1033 549
Fragments arranged by size:
1033 947 549
BsaAI yAC'GTr
Cuts at: 0 1436 2289 2529
Size: 1436 853 240
Fragments arranged by size:
1436 853 240
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 915 945 2529
Size: 915 30 1584
Fragments arranged by size:
1584 915 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 130 725 1294 2529
Size: 130 595 569 1235
Fragments arranged by size:
1235 595 569 130
BseYI C'CCAG_C
Cuts at: 0 218 2409 2529
Size: 218 2191 120
Fragments arranged by size:
2191 218 120
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1318 1639 2050 2392 2529
Size: 1318 321 411 342 137
Fragments arranged by size:
1318 411 342 321 137
BsiHKAI G_wGCw'C
Cuts at: 0 86 2225 2528 2529
Size: 86 2139 303 1
Fragments arranged by size:
2139 303 86 1
BsmBI CGTCTCn'nnnn_
Cuts at: 0 25 2036 2529
Size: 25 2011 493
Fragments arranged by size:
2011 493 25
Bsp1286I G_dGCh'C
Cuts at: 0 86 675 930 1330 1433 1521 2049 2062
Size: 86 589 255 400 103 88 528 13
Cuts at: 2062 2225 2504 2528 2529
Size: 163 279 24 1
Fragments arranged by size:
589 528 400 279 255 163 103 88
86 24 13 1
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 776 2364 2529
Size: 776 1588 165
Fragments arranged by size:
1588 776 165
BsrBI CCG'CTC
Cuts at: 0 53 72 2529
Size: 53 19 2457
Fragments arranged by size:
2457 53 19
BsrDI GCAATG_nn'
Cuts at: 0 1109 1904 2529
Size: 1109 795 625
Fragments arranged by size:
1109 795 625
BssSI C'ACGA_G
Cuts at: 0 2392 2529
Size: 2392 137
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1827 2529
Size: 1827 702
BstXI CCAn_nnnn'nTGG
Cuts at: 0 792 2529
Size: 792 1737
BstYI r'GATC_y
Cuts at: 0 74 800 849 1589 2090 2354 2529
Size: 74 726 49 740 501 264 175
Fragments arranged by size:
740 726 501 264 175 74 49
Bsu36I CC'TnA_GG
Cuts at: 0 184 2529
Size: 184 2345
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 96 528 2529
Size: 96 432 2001
Fragments arranged by size:
2001 432 96
BtsI GCAGTG_nn'
Cuts at: 0 1804 2049 2529
Size: 1804 245 480
Fragments arranged by size:
1804 480 245
EaeI y'GGCC_r
Cuts at: 0 101 308 1013 2208 2529
Size: 101 207 705 1195 321
Fragments arranged by size:
1195 705 321 207 101
EarI CTCTTCn'nnn_
Cuts at: 0 1119 1340 1628 2179 2255 2287 2529
Size: 1119 221 288 551 76 32 242
Fragments arranged by size:
1119 551 288 242 221 76 32
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1261 1603 2362 2529
Size: 1261 342 759 167
Fragments arranged by size:
1261 759 342 167
EcoICRI GAG'CTC
Cuts at: 0 84 2223 2529
Size: 84 2139 306
Fragments arranged by size:
2139 306 84
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 178 190 649 865 1297 1566 1609
Size: 49 129 12 459 216 432 269 43
Cuts at: 1609 1932 2129 2500 2529
Size: 323 197 371 29
Fragments arranged by size:
459 432 371 323 269 216 197 129
49 43 29 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 187 2529
Size: 187 2342
EcoO109I rG'GnC_Cy
Cuts at: 0 208 1482 1517 2058 2418 2529
Size: 208 1274 35 541 360 111
Fragments arranged by size:
1274 541 360 208 111 35
EcoRI G'AATT_C
Cuts at: 0 280 1186 2529
Size: 280 906 1343
Fragments arranged by size:
1343 906 280
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1194 2529
Size: 1194 1335
HaeII r_GCGC'y
Cuts at: 0 48 1501 2529
Size: 48 1453 1028
Fragments arranged by size:
1453 1028 48
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 662 694 917 949 1129 1161 1378 1410
Size: 662 32 223 32 180 32 217 32
Cuts at: 1410 1633 1665 2529
Size: 223 32 864
Fragments arranged by size:
864 662 223 223 217 180 32 32
32 32 32
KpnI G_GTAC'C
Cuts at: 0 1203 2529
Size: 1203 1326
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 696 1322 1997 2529
Size: 696 626 675 532
Fragments arranged by size:
696 675 626 532
MscI TGG'CCA
Cuts at: 0 310 1015 2529
Size: 310 705 1514
Fragments arranged by size:
1514 705 310
MslI CAynn'nnrTG
Cuts at: 0 1055 1694 1717 2287 2529
Size: 1055 639 23 570 242
Fragments arranged by size:
1055 639 570 242 23
MspA1I CmG'CkG
Cuts at: 0 96 222 394 792 2064 2107 2529
Size: 96 126 172 398 1272 43 422
Fragments arranged by size:
1272 422 398 172 126 96 43
NspI r_CATG'y
Cuts at: 0 1827 2205 2301 2529
Size: 1827 378 96 228
Fragments arranged by size:
1827 378 228 96
PfoI T'CCnGG_A
Cuts at: 0 2360 2529
Size: 2360 169
PmlI CAC'GTG
Cuts at: 0 2289 2529
Size: 2289 240
PpuMI rG'GwC_Cy
Cuts at: 0 1482 2418 2529
Size: 1482 936 111
Fragments arranged by size:
1482 936 111
PshAI GACnn'nnGTC
Cuts at: 0 229 394 2529
Size: 229 165 2135
Fragments arranged by size:
2135 229 165
PsiI TTA'TAA
Cuts at: 0 338 2529
Size: 338 2191
PspOMI G'GGCC_C
Cuts at: 0 1517 2058 2529
Size: 1517 541 471
Fragments arranged by size:
1517 541 471
PstI C_TGCA'G
Cuts at: 0 1079 1448 1571 1832 2039 2139 2529
Size: 1079 369 123 261 207 100 390
Fragments arranged by size:
1079 390 369 261 207 123 100
PvuII CAG'CTG
Cuts at: 0 96 222 394 792 2107 2529
Size: 96 126 172 398 1315 422
Fragments arranged by size:
1315 422 398 172 126 96
SacI G_AGCT'C
Cuts at: 0 86 2225 2529
Size: 86 2139 304
Fragments arranged by size:
2139 304 86
SapI GCTCTTCn'nnn_
Cuts at: 0 1628 2287 2529
Size: 1628 659 242
Fragments arranged by size:
1628 659 242
SfcI C'TryA_G
Cuts at: 0 405 1075 1444 1486 1567 1828 2035 2135
Size: 405 670 369 42 81 261 207 100
Cuts at: 2135 2337 2529
Size: 202 192
Fragments arranged by size:
670 405 369 261 207 202 192 100
81 42
SmaI CCC'GGG
Cuts at: 0 364 2529
Size: 364 2165
SmlI C'TyrA_G
Cuts at: 0 271 962 1995 2529
Size: 271 691 1033 534
Fragments arranged by size:
1033 691 534 271
SphI G_CATG'C
Cuts at: 0 2301 2529
Size: 2301 228
SspI AAT'ATT
Cuts at: 0 591 2529
Size: 591 1938
StuI AGG'CCT
Cuts at: 0 2266 2529
Size: 2266 263
StyI C'CwwG_G
Cuts at: 0 2471 2529
Size: 2471 58
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 275 1548 2299 2529
Size: 275 1273 751 230
Fragments arranged by size:
1273 751 275 230
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 841 873 915 947 2529
Size: 841 32 42 32 1582
Fragments arranged by size:
1582 841 42 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 123 303 1320 2529
Size: 123 180 1017 1209
Fragments arranged by size:
1209 1017 180 123
XmaI C'CCGG_G
Cuts at: 0 362 2529
Size: 362 2167
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AflII AleI AlfI
AlwNI ApaI ApoI AvaI BaeI BamHI BanI BanII
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaAI BsaXI BseRI BseYI
BsgI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrDI BssSI
BstAPI BstXI BstYI Bsu36I BtgZI BtsI EaeI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII
Hin4I KpnI MmeI MscI MslI MspA1I NspI PfoI
PmlI PpuMI PshAI PsiI PspOMI PstI PvuII SacI
SapI SfcI SmaI SmlI SphI SspI StuI StyI
TaqII TstI XcmI XmaI
Enzymes that do not cut:
AatII AclI AflIII AgeI AhdI AloI ApaLI AscI
AseI AsiSI AvrII BbeI BbsI BbvCI BcgI BfrBI
BmtI BsaI BsaBI BsaHI BsaWI BsiEI BsiWI BsmI
BspEI BspHI BsrFI BsrGI BssHII BstBI BstEII BstZ17I
BtgI ClaI CspCI DraI DraIII DrdI EagI EcoRV
FseI FspI FspAI HincII HindIII HpaI KasI MfeI
MluI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PciI PflMI PmeI PpiI
PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI
SrfI SwaI TatI Tth111I XbaI XhoI XmnI ZraI