(Circular) (Six-base) MAPSORT of: pFN21AE0546.seq Check: 5636 from: 1 to: 5596 pFN21AE0546 5596 bp With 182 enzymes: SgfI * October 20, 2009 10:49 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2494 2494 Size: 5596 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3406 278 Size: 53 83 186 936 1870 2468 Fragments arranged by size: 2468 1870 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2939 1937 Size: 1002 4594 Acc65I G'GTAC_C Cuts at: 2917 2917 Size: 5596 AclI AA'CG_TT Cuts at: 1563 3723 4096 1563 Size: 2160 373 3063 Fragments arranged by size: 3063 2160 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2429 3659 5043 5311 1632 Size: 797 1230 1384 268 1917 Fragments arranged by size: 1917 1384 1230 797 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5399 AflIII A'CryG_T Cuts at: 1203 2103 4495 5472 1203 Size: 900 2392 977 1327 Fragments arranged by size: 2392 1327 977 900 AgeI A'CCGG_T Cuts at: 4427 4427 Size: 5596 AhdI GACnn_n'nnGTC Cuts at: 2206 4325 2206 Size: 2119 3477 AleI CACnn'nnGTG Cuts at: 1166 2288 1166 Size: 1122 4474 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5596 AlwNI CAG_nnn'CTG Cuts at: 2509 3339 4911 2509 Size: 830 1572 3194 Fragments arranged by size: 3194 1572 830 ApaI G_GGCC'C Cuts at: 2085 2681 2854 2085 Size: 596 173 4827 Fragments arranged by size: 4827 596 173 ApaLI G'TGCA_C Cuts at: 3653 4809 3653 Size: 1156 4440 ApoI r'AATT_y Cuts at: 1493 2905 3131 3283 5392 1493 Size: 1412 226 152 2109 1697 Fragments arranged by size: 2109 1697 1412 226 152 AseI AT'TA_AT Cuts at: 160 1032 4150 160 Size: 872 3118 1606 Fragments arranged by size: 3118 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5596 AvaI C'yCGr_G Cuts at: 1870 1943 2127 2150 2334 2608 2921 1870 Size: 73 184 23 184 274 313 4545 Fragments arranged by size: 4545 313 274 184 184 73 23 BamHI G'GATC_C Cuts at: 2926 3293 2926 Size: 367 5229 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2123 2168 2573 2624 2917 Size: 325 217 627 336 45 405 51 293 Cuts at: 2917 4372 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 405 336 325 293 217 51 45 BanII G_rGCy'C Cuts at: 1391 2085 2439 2681 2691 2854 2915 1391 Size: 694 354 242 10 163 61 4072 Fragments arranged by size: 4072 694 354 242 163 61 10 BbeI G_GCGC'C Cuts at: 2172 2172 Size: 5596 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2452 928 Size: 971 553 4072 Fragments arranged by size: 4072 971 553 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3787 3821 1903 Size: 34 1850 34 3678 Fragments arranged by size: 3678 1850 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3488 4698 3488 Size: 1210 4386 BclI T'GATC_A Cuts at: 1548 1548 Size: 5596 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2268 4207 136 Size: 107 122 71 631 605 596 1939 1525 Fragments arranged by size: 1939 1525 631 605 596 122 107 71 BglII A'GATC_T Cuts at: 5591 5591 Size: 5596 BlpI GC'TnA_GC Cuts at: 3013 3013 Size: 5596 Bme1580I G_kGCm'C Cuts at: 1790 2085 2576 2681 2854 3657 4813 1790 Size: 295 491 105 173 803 1156 2573 Fragments arranged by size: 2573 1156 803 491 295 173 105 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5596 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3327 4285 442 Size: 416 53 83 399 24 1910 958 1753 Fragments arranged by size: 1910 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5596 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2531 4256 1239 Size: 1292 1725 2579 Fragments arranged by size: 2579 1725 1292 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2474 2541 3721 4586 4884 2474 Size: 67 1180 865 298 3186 Fragments arranged by size: 3186 1180 865 298 67 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2041 2559 4259 882 Size: 617 542 518 1700 2219 Fragments arranged by size: 2219 1700 617 542 518 BsaAI yAC'GTr Cuts at: 493 1204 2161 2290 2745 2773 3387 493 Size: 711 957 129 455 28 614 2702 Fragments arranged by size: 2702 957 711 614 455 129 28 BsaBI GATnn'nnATC Cuts at: 5590 5590 Size: 5596 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2169 2207 3403 3785 Size: 53 83 186 936 636 38 1196 382 Cuts at: 3785 4420 275 Size: 635 1451 Fragments arranged by size: 1451 1196 936 636 635 382 186 83 53 38 BsaWI w'CCGG_w Cuts at: 2397 4029 4427 4701 4848 5259 2397 Size: 1632 398 274 147 411 2734 Fragments arranged by size: 2734 1632 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2810 2840 1178 Size: 30 165 30 1407 30 3934 Fragments arranged by size: 3934 1407 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5596 BseYI C'CCAG_C Cuts at: 2077 2231 4799 5295 2077 Size: 154 2568 496 2378 Fragments arranged by size: 2568 2378 496 154 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5023 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2457 3046 3807 4835 1540 Size: 275 186 456 589 761 1028 2301 Fragments arranged by size: 2301 1028 761 589 456 275 186 BsiHKAI G_wGCw'C Cuts at: 2022 2385 2691 2698 3657 3742 4813 2022 Size: 363 306 7 959 85 1071 2805 Fragments arranged by size: 2805 1071 959 363 306 85 7 BsmI GAATG_Cn' Cuts at: 3107 3200 3107 Size: 93 5503 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2022 2085 2385 2439 2576 2681 2691 Size: 399 232 63 300 54 137 105 10 Cuts at: 2691 2698 2854 2915 3657 3742 4813 1391 Size: 7 156 61 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 300 232 156 137 105 85 63 61 54 10 7 BspEI T'CCGG_A Cuts at: 2397 5259 2397 Size: 2862 2734 BspHI T'CATG_A Cuts at: 3485 5215 3485 Size: 1730 3866 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2494 2951 844 Size: 1650 457 3489 Fragments arranged by size: 3489 1650 457 BsrBI CCG'CTC Cuts at: 1394 1823 2481 3483 1394 Size: 429 658 1002 3507 Fragments arranged by size: 3507 1002 658 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2736 4091 4265 65 Size: 1183 1488 1355 174 1396 Fragments arranged by size: 1488 1396 1355 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2452 4240 4427 1700 Size: 110 642 1788 187 2869 Fragments arranged by size: 2869 1788 642 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5596 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5596 BssSI C'ACGA_G Cuts at: 3656 4668 3656 Size: 1012 4584 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5596 BstBI TT'CG_AA Cuts at: 4400 4400 Size: 5596 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5596 BstXI CCAn_nnnn'nTGG Cuts at: 2050 2621 2716 2050 Size: 571 95 4930 Fragments arranged by size: 4930 571 95 BstYI r'GATC_y Cuts at: 1972 2719 2926 3293 3678 3695 5147 5233 5245 Size: 747 207 367 385 17 1452 86 12 Cuts at: 5245 5591 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 747 385 367 346 207 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2620 513 Size: 552 939 616 3489 Fragments arranged by size: 3489 939 616 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4857 Fragments arranged by size: 4857 683 56 BtsI GCAGTG_nn' Cuts at: 3112 3906 3926 3112 Size: 794 20 4782 Fragments arranged by size: 4782 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5596 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5561 DraI TTT'AAA Cuts at: 2900 2900 Size: 5596 DraIII CAC_nnn'GTG Cuts at: 2046 2293 2425 2046 Size: 247 132 5217 Fragments arranged by size: 5217 247 132 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4603 809 Size: 507 3287 1802 Fragments arranged by size: 3287 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2362 2454 2811 Size: 54 1425 50 275 18 532 92 357 Cuts at: 2811 3043 3932 5561 8 Size: 232 889 1629 43 Fragments arranged by size: 1629 1425 889 532 357 275 232 92 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2454 3043 1537 Size: 275 642 589 4090 Fragments arranged by size: 4090 642 589 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2184 2679 3526 1353 Size: 831 495 847 3423 Fragments arranged by size: 3423 847 831 495 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2069 4183 4557 4703 387 Size: 1064 138 480 2114 374 146 1280 Fragments arranged by size: 2114 1280 1064 480 374 146 138 EcoICRI GAG'CTC Cuts at: 2689 2689 Size: 5596 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2429 2531 3659 4256 5043 5311 1239 Size: 393 797 102 1128 597 787 268 1524 Fragments arranged by size: 1524 1128 797 787 597 393 268 102 EcoNI CCTnn'n_nnAGG Cuts at: 2657 2657 Size: 5596 EcoO109I rG'GnC_Cy Cuts at: 2081 2677 2851 2081 Size: 596 174 4826 Fragments arranged by size: 4826 596 174 EcoRI G'AATT_C Cuts at: 2905 3283 2905 Size: 378 5218 FspI TGC'GCA Cuts at: 4102 4102 Size: 5596 HaeII r_GCGC'y Cuts at: 1678 2033 2158 2172 4743 1678 Size: 355 125 14 2571 2531 Fragments arranged by size: 2571 2531 355 125 14 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2192 2224 2537 2569 4237 Size: 32 135 32 620 32 313 32 1668 Cuts at: 4237 4269 4311 4343 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1668 620 313 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2632 2940 3186 669 Size: 988 281 694 308 246 3079 Fragments arranged by size: 3079 988 694 308 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5596 HpaI GTT'AAC Cuts at: 3186 3186 Size: 5596 KasI G'GCGC_C Cuts at: 2168 2168 Size: 5596 KpnI G_GTAC'C Cuts at: 2921 2921 Size: 5596 MfeI C'AATT_G Cuts at: 3195 3195 Size: 5596 MluI A'CGCG_T Cuts at: 2103 5472 2103 Size: 3369 2227 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4710 4894 1305 Size: 3405 184 2007 Fragments arranged by size: 3405 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2364 10 Size: 54 1425 343 532 3242 Fragments arranged by size: 3242 1425 532 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2288 2619 3554 3913 4072 518 Size: 648 1122 331 935 359 159 2042 Fragments arranged by size: 2042 1122 935 648 359 331 159 MspA1I CmG'CkG Cuts at: 1606 2539 3012 3689 4837 5082 1606 Size: 933 473 677 1148 245 2120 Fragments arranged by size: 2120 1148 933 677 473 245 NarI GG'CG_CC Cuts at: 2169 2169 Size: 5596 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4105 Fragments arranged by size: 4105 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5596 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5596 NotI GC'GGCC_GC Cuts at: 3043 3043 Size: 5596 NspI r_CATG'y Cuts at: 1132 1229 2638 2954 4499 1132 Size: 97 1409 316 1545 2229 Fragments arranged by size: 2229 1545 1409 316 97 PasI CC'CwG_GG Cuts at: 1348 2875 1348 Size: 1527 4069 PciI A'CATG_T Cuts at: 4495 4495 Size: 5596 PflMI CCAn_nnn'nTGG Cuts at: 1108 2046 2542 1108 Size: 938 496 4162 Fragments arranged by size: 4162 938 496 PmeI GTTT'AAAC Cuts at: 2900 2900 Size: 5596 PmlI CAC'GTG Cuts at: 2290 2745 2773 2290 Size: 455 28 5113 Fragments arranged by size: 5113 455 28 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3665 3697 5178 5210 3665 Size: 32 1481 32 4051 Fragments arranged by size: 4051 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5596 PsiI TTA'TAA Cuts at: 3166 3166 Size: 5596 PspOMI G'GGCC_C Cuts at: 2081 2677 2850 2081 Size: 596 173 4827 Fragments arranged by size: 4827 596 173 PspXI vC'TCGA_Gb Cuts at: 2150 2150 Size: 5596 PstI C_TGCA'G Cuts at: 830 2948 830 Size: 2118 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5596 PvuII CAG'CTG Cuts at: 2539 2539 Size: 5596 SacI G_AGCT'C Cuts at: 2691 2691 Size: 5596 SalI G'TCGA_C Cuts at: 1936 2938 1936 Size: 1002 4594 SapI GCTCTTCn'nnn_ Cuts at: 2679 2679 Size: 5596 SbfI CC_TGCA'GG Cuts at: 2948 2948 Size: 5596 ScaI AGT'ACT Cuts at: 3844 5556 3844 Size: 1712 3884 SfcI C'TryA_G Cuts at: 826 1045 2944 4079 4760 4951 826 Size: 219 1899 1135 681 191 1471 Fragments arranged by size: 1899 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2170 2170 Size: 5596 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5596 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5596 SmaI CCC'GGG Cuts at: 1872 2336 2923 1872 Size: 464 587 4545 Fragments arranged by size: 4545 587 464 SmlI C'TyrA_G Cuts at: 820 1017 1943 2150 2489 2556 3700 4601 4863 Size: 197 926 207 339 67 1144 901 262 Cuts at: 4863 820 Size: 1553 Fragments arranged by size: 1553 1144 926 901 339 262 207 197 67 SnaBI TAC'GTA Cuts at: 493 3387 493 Size: 2894 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5596 SphI G_CATG'C Cuts at: 1132 2954 1132 Size: 1822 3774 SspI AAT'ATT Cuts at: 5 52 3520 5 Size: 47 3468 2081 Fragments arranged by size: 3468 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5596 StyI C'CwwG_G Cuts at: 513 1065 2004 2778 3035 513 Size: 552 939 774 257 3074 Fragments arranged by size: 3074 939 774 552 257 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3623 3640 3793 3978 4423 325 Size: 1255 2043 17 153 185 445 1498 Fragments arranged by size: 2043 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3842 5554 96 Size: 274 80 33 51 1443 1865 1712 138 Fragments arranged by size: 1865 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2338 2370 5556 5588 2338 Size: 32 3186 32 2346 Fragments arranged by size: 3186 2346 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5596 XbaI T'CTAG_A Cuts at: 2932 2932 Size: 5596 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2047 2047 Size: 5596 XhoI C'TCGA_G Cuts at: 1943 2150 1943 Size: 207 5389 XmaI C'CCGG_G Cuts at: 1870 2334 2921 1870 Size: 464 587 4545 Fragments arranged by size: 4545 587 464 XmnI GAAnn'nnTTC Cuts at: 3725 3725 Size: 5596 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3404 276 Size: 53 83 186 936 1870 2468 Fragments arranged by size: 2468 1870 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PpuMI PsrI RsrII SacII SanDI SexAI SfiI SrfI SwaI