(Circular) (Six-base) MAPSORT of: pFN21AB7646.seq Check: 3434 from: 1 to: 5875 pFN21AB7646 5875 bp With 182 enzymes: SgfI * February 27, 2009 14:45 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3685 278 Size: 53 83 186 936 2149 2468 Fragments arranged by size: 2468 2149 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3218 1937 Size: 1281 4594 Acc65I G'GTAC_C Cuts at: 3196 3196 Size: 5875 AclI AA'CG_TT Cuts at: 1563 4002 4375 1563 Size: 2439 373 3063 Fragments arranged by size: 3063 2439 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3104 3938 5322 5590 1632 Size: 1472 834 1384 268 1917 Fragments arranged by size: 1917 1472 1384 834 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5678 AflIII A'CryG_T Cuts at: 1203 4774 5751 1203 Size: 3571 977 1327 Fragments arranged by size: 3571 1327 977 AgeI A'CCGG_T Cuts at: 4706 4706 Size: 5875 AhdI GACnn_n'nnGTC Cuts at: 3156 4604 3156 Size: 1448 4427 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5875 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2331 1184 Size: 1147 4728 AlwNI CAG_nnn'CTG Cuts at: 2812 2989 3618 5190 2812 Size: 177 629 1572 3497 Fragments arranged by size: 3497 1572 629 177 ApaLI G'TGCA_C Cuts at: 3932 5088 3932 Size: 1156 4719 ApoI r'AATT_y Cuts at: 1493 3184 3410 3562 5671 1493 Size: 1691 226 152 2109 1697 Fragments arranged by size: 2109 1697 1691 226 152 AseI AT'TA_AT Cuts at: 160 1032 4429 160 Size: 872 3397 1606 Fragments arranged by size: 3397 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5875 AvaI C'yCGr_G Cuts at: 1870 1943 3200 1870 Size: 73 1257 4545 Fragments arranged by size: 4545 1257 73 BamHI G'GATC_C Cuts at: 3205 3572 3205 Size: 367 5508 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3196 4651 618 Size: 325 217 627 1409 1455 1842 Fragments arranged by size: 1842 1455 1409 627 325 217 BanII G_rGCy'C Cuts at: 1391 2763 3194 1391 Size: 1372 431 4072 Fragments arranged by size: 4072 1372 431 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2419 2793 3027 3070 3089 928 Size: 971 520 374 234 43 19 3714 Fragments arranged by size: 3714 971 520 374 234 43 19 BbvCI CC'TCA_GC Cuts at: 3122 3122 Size: 5875 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4066 4100 1903 Size: 34 2129 34 3678 Fragments arranged by size: 3678 2129 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2528 3767 4977 2528 Size: 1239 1210 3426 Fragments arranged by size: 3426 1239 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5875 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4486 136 Size: 107 122 71 631 605 2814 1525 Fragments arranged by size: 2814 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2104 5870 2104 Size: 3766 2109 BlpI GC'TnA_GC Cuts at: 3292 3292 Size: 5875 Bme1580I G_kGCm'C Cuts at: 1790 2752 3130 3936 5092 1790 Size: 962 378 806 1156 2573 Fragments arranged by size: 2573 1156 962 806 378 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5875 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2544 2643 3606 Size: 416 53 83 399 24 1127 99 963 Cuts at: 3606 4564 442 Size: 958 1753 Fragments arranged by size: 1753 1127 963 958 416 399 99 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5875 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2708 2708 Size: 5875 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2406 4535 1239 Size: 1167 2129 2579 Fragments arranged by size: 2579 2129 1167 Bpu10I CC'TnA_GC Cuts at: 3122 3122 Size: 5875 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2012 3070 4000 4865 5163 2012 Size: 1058 930 865 298 2724 Fragments arranged by size: 2724 1058 930 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2052 3164 4538 882 Size: 617 553 1112 1374 2219 Fragments arranged by size: 2219 1374 1112 617 553 BsaAI yAC'GTr Cuts at: 493 1204 2072 3666 493 Size: 711 868 1594 2702 Fragments arranged by size: 2702 1594 868 711 BsaBI GATnn'nnATC Cuts at: 5869 5869 Size: 5875 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3682 4064 4699 275 Size: 53 83 186 936 2149 382 635 1451 Fragments arranged by size: 2149 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2531 4308 4706 4980 5127 5538 2531 Size: 1777 398 274 147 411 2868 Fragments arranged by size: 2868 1777 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5650 Fragments arranged by size: 5650 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5875 BseYI C'CCAG_C Cuts at: 5078 5574 5078 Size: 496 5379 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5302 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3325 4086 5114 1540 Size: 275 186 1324 761 1028 2301 Fragments arranged by size: 2301 1324 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2763 3936 4021 5092 2763 Size: 1173 85 1071 3546 Fragments arranged by size: 3546 1173 1071 85 BsmI GAATG_Cn' Cuts at: 3386 3479 3386 Size: 93 5782 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2752 2763 3130 3194 3936 4021 5092 Size: 399 962 11 367 64 742 85 1071 Cuts at: 5092 1391 Size: 2174 Fragments arranged by size: 2174 1071 962 742 399 367 85 64 11 BspEI T'CCGG_A Cuts at: 2531 5538 2531 Size: 3007 2868 BspHI T'CATG_A Cuts at: 2394 3764 5494 2394 Size: 1370 1730 2775 Fragments arranged by size: 2775 1730 1370 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3230 844 Size: 2386 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3762 1394 Size: 429 1939 3507 Fragments arranged by size: 3507 1939 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4370 4544 65 Size: 1183 3122 174 1396 Fragments arranged by size: 3122 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3155 4519 4706 1700 Size: 110 1345 1364 187 2869 Fragments arranged by size: 2869 1364 1345 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5875 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5875 BssSI C'ACGA_G Cuts at: 3935 4947 3935 Size: 1012 4863 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5875 BstBI TT'CG_AA Cuts at: 4679 4679 Size: 5875 BstEII G'GTnAC_C Cuts at: 1184 2555 2801 2824 1184 Size: 1371 246 23 4235 Fragments arranged by size: 4235 1371 246 23 BstYI r'GATC_y Cuts at: 1972 2104 2985 3205 3572 3957 3974 5426 5512 Size: 132 881 220 367 385 17 1452 86 Cuts at: 5512 5524 5870 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 881 385 367 346 220 132 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4384 Fragments arranged by size: 4384 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5136 Fragments arranged by size: 5136 683 56 BtsI GCAGTG_nn' Cuts at: 3391 4185 4205 3391 Size: 794 20 5061 Fragments arranged by size: 5061 794 20 ClaI AT'CG_AT Cuts at: 1554 2946 1554 Size: 1392 4483 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2533 2568 529 Size: 35 1969 35 3836 Fragments arranged by size: 3836 1969 35 35 DraI TTT'AAA Cuts at: 3179 3179 Size: 5875 DraIII CAC_nnn'GTG Cuts at: 2582 2582 Size: 5875 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3074 4882 809 Size: 507 1758 1808 1802 Fragments arranged by size: 1808 1802 1758 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3322 4211 5840 Size: 54 1425 50 275 18 1492 889 1629 Cuts at: 5840 8 Size: 43 Fragments arranged by size: 1629 1492 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3322 1537 Size: 275 1510 4090 Fragments arranged by size: 4090 1510 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2811 3805 1353 Size: 1458 994 3423 Fragments arranged by size: 3423 1458 994 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2445 2740 4462 4836 4982 387 Size: 1064 138 856 295 1722 374 146 1280 Fragments arranged by size: 1722 1280 1064 856 374 295 146 138 EcoICRI GAG'CTC Cuts at: 2761 2761 Size: 5875 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2406 3104 3938 4535 5322 5590 1239 Size: 393 774 698 834 597 787 268 1524 Fragments arranged by size: 1524 834 787 774 698 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2193 2795 3052 2193 Size: 602 257 5016 Fragments arranged by size: 5016 602 257 EcoRI G'AATT_C Cuts at: 3184 3562 3184 Size: 378 5497 EcoRV GAT'ATC Cuts at: 2944 2944 Size: 5875 FspI TGC'GCA Cuts at: 4381 4381 Size: 5875 HaeII r_GCGC'y Cuts at: 1678 5022 1678 Size: 3344 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2977 3009 3142 3174 4516 Size: 32 135 32 1405 32 133 32 1342 Cuts at: 4516 4548 4590 4622 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1405 1342 135 133 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3219 3465 669 Size: 988 281 1281 246 3079 Fragments arranged by size: 3079 1281 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5875 HpaI GTT'AAC Cuts at: 3465 3465 Size: 5875 KpnI G_GTAC'C Cuts at: 3200 3200 Size: 5875 MfeI C'AATT_G Cuts at: 3474 3474 Size: 5875 MluI A'CGCG_T Cuts at: 5751 5751 Size: 5875 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 1995 4989 5173 1305 Size: 690 2994 184 2007 Fragments arranged by size: 2994 2007 690 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4053 Fragments arranged by size: 4053 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2313 2399 3833 4192 4351 518 Size: 648 843 304 86 1434 359 159 2042 Fragments arranged by size: 2042 1434 843 648 359 304 159 86 MspA1I CmG'CkG Cuts at: 1606 3121 3291 3968 5116 5361 1606 Size: 1515 170 677 1148 245 2120 Fragments arranged by size: 2120 1515 1148 677 245 170 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4384 Fragments arranged by size: 4384 939 552 NdeI CA'TA_TG Cuts at: 387 2128 387 Size: 1741 4134 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5875 NotI GC'GGCC_GC Cuts at: 3322 3322 Size: 5875 NspI r_CATG'y Cuts at: 1132 1229 2312 2497 2506 3092 3233 4778 1132 Size: 97 1083 185 9 586 141 1545 2229 Fragments arranged by size: 2229 1545 1083 586 185 141 97 9 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5875 PciI A'CATG_T Cuts at: 4774 4774 Size: 5875 PflMI CCAn_nnn'nTGG Cuts at: 1108 2582 2812 1108 Size: 1474 230 4171 Fragments arranged by size: 4171 1474 230 PmeI GTTT'AAAC Cuts at: 3179 3179 Size: 5875 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3944 3976 5457 5489 3944 Size: 32 1481 32 4330 Fragments arranged by size: 4330 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5875 PsiI TTA'TAA Cuts at: 3445 3445 Size: 5875 PstI C_TGCA'G Cuts at: 830 3227 830 Size: 2397 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5875 PvuII CAG'CTG Cuts at: 3121 3121 Size: 5875 SacI G_AGCT'C Cuts at: 2763 2763 Size: 5875 SalI G'TCGA_C Cuts at: 1936 3217 1936 Size: 1281 4594 SbfI CC_TGCA'GG Cuts at: 3227 3227 Size: 5875 ScaI AGT'ACT Cuts at: 4123 5835 4123 Size: 1712 4163 SexAI A'CCwGG_T Cuts at: 2578 2578 Size: 5875 SfcI C'TryA_G Cuts at: 826 1045 3223 4358 5039 5230 826 Size: 219 2178 1135 681 191 1471 Fragments arranged by size: 2178 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5875 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5875 SmaI CCC'GGG Cuts at: 1872 3202 1872 Size: 1330 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2027 3049 3979 4880 5142 820 Size: 197 926 84 1022 930 901 262 1553 Fragments arranged by size: 1553 1022 930 926 901 262 197 84 SnaBI TAC'GTA Cuts at: 493 3666 493 Size: 3173 2702 SpeI A'CTAG_T Cuts at: 152 2279 152 Size: 2127 3748 SphI G_CATG'C Cuts at: 1132 2506 3233 1132 Size: 1374 727 3774 Fragments arranged by size: 3774 1374 727 SspI AAT'ATT Cuts at: 5 52 2212 3799 5 Size: 47 2160 1587 2081 Fragments arranged by size: 2160 2081 1587 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5875 StyI C'CwwG_G Cuts at: 513 1065 2004 3314 513 Size: 552 939 1310 3074 Fragments arranged by size: 3074 1310 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2376 3902 3919 4072 4257 4702 325 Size: 1255 796 1526 17 153 185 445 1498 Fragments arranged by size: 1526 1498 1255 796 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2046 4121 5833 Size: 274 80 33 51 1443 69 2075 1712 Cuts at: 5833 96 Size: 138 Fragments arranged by size: 2075 1712 1443 274 138 80 69 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2629 2661 5835 5867 2629 Size: 32 3174 32 2637 Fragments arranged by size: 3174 2637 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5875 XbaI T'CTAG_A Cuts at: 3211 3211 Size: 5875 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2223 2223 Size: 5875 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5875 XmaI C'CCGG_G Cuts at: 1870 3200 1870 Size: 1330 4545 XmnI GAAnn'nnTTC Cuts at: 4004 4004 Size: 5875 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3683 276 Size: 53 83 186 936 2149 2468 Fragments arranged by size: 2468 2149 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoO109I FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI