(Circular) (Six-base) MAPSORT of: pFN21AB7893.seq Check: 728 from: 1 to: 6469 pFN21AB7893 6469 bp With 182 enzymes: SgfI * October 20, 2009 10:56 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4279 278 Size: 53 83 186 936 2743 2468 Fragments arranged by size: 2743 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3812 1937 Size: 1875 4594 Acc65I G'GTAC_C Cuts at: 3790 3790 Size: 6469 AclI AA'CG_TT Cuts at: 1563 4596 4969 1563 Size: 3033 373 3063 Fragments arranged by size: 3063 3033 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2204 2269 4532 5916 6184 1632 Size: 572 65 2263 1384 268 1917 Fragments arranged by size: 2263 1917 1384 572 268 65 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6272 AflIII A'CryG_T Cuts at: 1203 5368 6345 1203 Size: 4165 977 1327 Fragments arranged by size: 4165 1327 977 AgeI A'CCGG_T Cuts at: 5300 5300 Size: 6469 AhdI GACnn_n'nnGTC Cuts at: 5198 5198 Size: 6469 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6469 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6469 AlwNI CAG_nnn'CTG Cuts at: 4212 5784 4212 Size: 1572 4897 ApaI G_GGCC'C Cuts at: 2078 2078 Size: 6469 ApaLI G'TGCA_C Cuts at: 3393 4526 5682 3393 Size: 1133 1156 4180 Fragments arranged by size: 4180 1156 1133 ApoI r'AATT_y Cuts at: 1493 3140 3505 3778 4004 4156 6265 1493 Size: 1647 365 273 226 152 2109 1697 Fragments arranged by size: 2109 1697 1647 365 273 226 152 AseI AT'TA_AT Cuts at: 160 1032 5023 160 Size: 872 3991 1606 Fragments arranged by size: 3991 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6469 AvaI C'yCGr_G Cuts at: 1870 1943 2438 2466 2645 3093 3546 3794 1870 Size: 73 495 28 179 448 453 248 4545 Fragments arranged by size: 4545 495 453 448 248 179 73 28 BamHI G'GATC_C Cuts at: 3799 4166 3799 Size: 367 6102 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2099 2618 3524 3790 5245 Size: 325 217 627 312 519 906 266 1455 Cuts at: 5245 618 Size: 1842 Fragments arranged by size: 1842 1455 906 627 519 325 312 266 217 BanII G_rGCy'C Cuts at: 1391 2045 2069 2078 2331 2439 2652 2706 3788 Size: 654 24 9 253 108 213 54 1082 Cuts at: 3788 1391 Size: 4072 Fragments arranged by size: 4072 1082 654 253 213 108 54 24 9 BbeI G_GCGC'C Cuts at: 2622 2622 Size: 6469 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5498 BbvCI CC'TCA_GC Cuts at: 2157 2157 Size: 6469 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4660 4694 1903 Size: 34 2723 34 3678 Fragments arranged by size: 3678 2723 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4361 5571 4361 Size: 1210 5259 BclI T'GATC_A Cuts at: 1548 3039 1548 Size: 1491 4978 BfrBI ATG'CAT Cuts at: 3264 3264 Size: 6469 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2073 2082 5080 Size: 107 122 71 631 605 401 9 2998 Cuts at: 5080 136 Size: 1525 Fragments arranged by size: 2998 1525 631 605 401 122 107 71 9 BglII A'GATC_T Cuts at: 6464 6464 Size: 6469 BlpI GC'TnA_GC Cuts at: 3886 3886 Size: 6469 Bme1580I G_kGCm'C Cuts at: 1790 2078 2142 3397 4530 5686 1790 Size: 288 64 1255 1133 1156 2573 Fragments arranged by size: 2573 1255 1156 1133 288 64 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6469 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2073 3761 4200 Size: 416 53 83 399 24 656 1688 439 Cuts at: 4200 5158 442 Size: 958 1753 Fragments arranged by size: 1753 1688 958 656 439 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6469 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3580 3580 Size: 6469 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2008 2111 2315 2633 2954 2987 3020 3224 Size: 769 103 204 318 321 33 33 204 Cuts at: 3224 3550 5129 1239 Size: 326 1579 2579 Fragments arranged by size: 2579 1579 769 326 321 318 204 204 103 33 33 Bpu10I CC'TnA_GC Cuts at: 2157 3299 2157 Size: 1142 5327 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2585 2828 4594 5459 5757 2585 Size: 243 1766 865 298 3297 Fragments arranged by size: 3297 1766 865 298 243 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2438 2625 3485 5132 882 Size: 617 939 187 860 1647 2219 Fragments arranged by size: 2219 1647 939 860 617 187 BsaAI yAC'GTr Cuts at: 493 1204 4260 493 Size: 711 3056 2702 Fragments arranged by size: 3056 2702 711 BsaBI GATnn'nnATC Cuts at: 6463 6463 Size: 6469 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2619 4276 4658 5293 Size: 53 83 186 936 1086 1657 382 635 Cuts at: 5293 275 Size: 1451 Fragments arranged by size: 1657 1451 1086 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3397 3447 4902 5300 5574 5721 6132 3397 Size: 50 1455 398 274 147 411 3734 Fragments arranged by size: 3734 1455 411 398 274 147 50 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2258 2288 2504 2534 3527 Size: 30 165 30 855 30 216 30 993 Cuts at: 3527 3557 1178 Size: 30 4090 Fragments arranged by size: 4090 993 855 216 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2525 2801 3553 3604 1186 Size: 1339 276 752 51 4051 Fragments arranged by size: 4051 1339 752 276 51 BseYI C'CCAG_C Cuts at: 2335 3669 5672 6168 2335 Size: 1334 2003 496 2636 Fragments arranged by size: 2636 2003 1334 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5896 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3919 4680 5708 1540 Size: 275 186 1918 761 1028 2301 Fragments arranged by size: 2301 1918 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2706 2760 3397 4530 4615 5686 2706 Size: 54 637 1133 85 1071 3489 Fragments arranged by size: 3489 1133 1071 637 85 54 BsiWI C'GTAC_G Cuts at: 2319 2319 Size: 6469 BsmI GAATG_Cn' Cuts at: 3570 3980 4073 3570 Size: 410 93 5966 Fragments arranged by size: 5966 410 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2801 3474 2801 Size: 673 5796 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2045 2069 2078 2142 2331 2439 2652 Size: 399 255 24 9 64 189 108 213 Cuts at: 2652 2706 2760 3397 3788 4530 4615 5686 1391 Size: 54 54 637 391 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 637 399 391 255 213 189 108 85 64 54 54 24 9 BspEI T'CCGG_A Cuts at: 3447 6132 3447 Size: 2685 3784 BspHI T'CATG_A Cuts at: 4358 6088 4358 Size: 1730 4739 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2735 3258 3600 3824 844 Size: 1891 523 342 224 3489 Fragments arranged by size: 3489 1891 523 342 224 BsrBI CCG'CTC Cuts at: 1394 1823 4356 1394 Size: 429 2533 3507 Fragments arranged by size: 3507 2533 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2227 4964 5138 65 Size: 1183 979 2737 174 1396 Fragments arranged by size: 2737 1396 1183 979 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3717 5113 5300 1700 Size: 110 1907 1396 187 2869 Fragments arranged by size: 2869 1907 1396 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6469 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6469 BssSI C'ACGA_G Cuts at: 4529 5541 4529 Size: 1012 5457 BstAPI GCAn_nnn'nTGC Cuts at: 794 3703 794 Size: 2909 3560 BstBI TT'CG_AA Cuts at: 5273 5273 Size: 6469 BstEII G'GTnAC_C Cuts at: 1184 3341 1184 Size: 2157 4312 BstYI r'GATC_y Cuts at: 1972 3799 4166 4551 4568 6020 6106 6118 6464 Size: 1827 367 385 17 1452 86 12 346 Cuts at: 6464 1972 Size: 1977 Fragments arranged by size: 1977 1827 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2418 513 Size: 552 939 414 4564 Fragments arranged by size: 4564 939 552 414 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3332 487 Size: 683 56 2106 3624 Fragments arranged by size: 3624 2106 683 56 BtsI GCAGTG_nn' Cuts at: 2819 3985 4779 4799 2819 Size: 1166 794 20 4489 Fragments arranged by size: 4489 1166 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6469 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6434 DraI TTT'AAA Cuts at: 3773 3773 Size: 6469 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2815 3596 3706 5476 809 Size: 507 1499 781 110 1770 1802 Fragments arranged by size: 1802 1770 1499 781 507 110 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3475 3719 3916 Size: 54 1425 50 275 18 1645 244 197 Cuts at: 3916 4805 6434 8 Size: 889 1629 43 Fragments arranged by size: 1645 1629 1425 889 275 244 197 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3916 1537 Size: 275 2104 4090 Fragments arranged by size: 4090 2104 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2711 3404 4399 1353 Size: 1358 693 995 3423 Fragments arranged by size: 3423 1358 995 693 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2467 3308 5056 5430 5576 387 Size: 1064 138 878 841 1748 374 146 1280 Fragments arranged by size: 1748 1280 1064 878 841 374 146 138 EcoICRI GAG'CTC Cuts at: 2704 2704 Size: 6469 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2008 2111 2204 2269 2315 2633 2954 Size: 393 376 103 93 65 46 318 321 Cuts at: 2954 2987 3020 3224 3550 4532 5129 5916 6184 Size: 33 33 204 326 982 597 787 268 Cuts at: 6184 1239 Size: 1524 Fragments arranged by size: 1524 982 787 597 393 376 326 321 318 268 204 103 93 65 46 33 33 EcoNI CCTnn'n_nnAGG Cuts at: 3203 3203 Size: 6469 EcoO109I rG'GnC_Cy Cuts at: 2075 2950 2962 3214 2075 Size: 875 12 252 5330 Fragments arranged by size: 5330 875 252 12 EcoRI G'AATT_C Cuts at: 3778 4156 3778 Size: 378 6091 FspI TGC'GCA Cuts at: 2387 4975 2387 Size: 2588 3881 HaeII r_GCGC'y Cuts at: 1678 2181 2208 2622 5616 1678 Size: 503 27 414 2994 2531 Fragments arranged by size: 2994 2531 503 414 27 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2226 2258 2290 5110 5142 Size: 32 135 32 654 32 32 2820 32 Cuts at: 5142 5184 5216 1373 Size: 42 32 2626 Fragments arranged by size: 2820 2626 654 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3733 3813 4059 669 Size: 988 281 1795 80 246 3079 Fragments arranged by size: 3079 1795 988 281 246 80 HindIII A'AGCT_T Cuts at: 748 748 Size: 6469 HpaI GTT'AAC Cuts at: 4059 4059 Size: 6469 KasI G'GCGC_C Cuts at: 2618 2618 Size: 6469 KpnI G_GTAC'C Cuts at: 3794 3794 Size: 6469 MfeI C'AATT_G Cuts at: 4068 4068 Size: 6469 MluI A'CGCG_T Cuts at: 6345 6345 Size: 6469 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2301 3780 5583 5767 1305 Size: 996 1479 1803 184 2007 Fragments arranged by size: 2007 1803 1479 996 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4647 Fragments arranged by size: 4647 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3351 4427 4786 4945 518 Size: 648 2185 1076 359 159 2042 Fragments arranged by size: 2185 2042 1076 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2156 2335 2641 2836 3007 3103 3885 4562 Size: 550 179 306 195 171 96 782 677 Cuts at: 4562 5710 5955 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 782 677 550 306 245 195 179 171 96 NarI GG'CG_CC Cuts at: 2619 2619 Size: 6469 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4978 Fragments arranged by size: 4978 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6469 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6469 NotI GC'GGCC_GC Cuts at: 3916 3916 Size: 6469 NsiI A_TGCA'T Cuts at: 3266 3266 Size: 6469 NspI r_CATG'y Cuts at: 1132 1229 2749 3268 3827 5372 1132 Size: 97 1520 519 559 1545 2229 Fragments arranged by size: 2229 1545 1520 559 519 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6469 PciI A'CATG_T Cuts at: 5368 5368 Size: 6469 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6469 PfoI T'CCnGG_A Cuts at: 2024 2408 2655 2751 2931 2946 2024 Size: 384 247 96 180 15 5547 Fragments arranged by size: 5547 384 247 180 96 15 PmeI GTTT'AAAC Cuts at: 3773 3773 Size: 6469 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4538 4570 6051 6083 4538 Size: 32 1481 32 4924 Fragments arranged by size: 4924 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2950 2962 2950 Size: 12 6457 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6469 PsiI TTA'TAA Cuts at: 4039 4039 Size: 6469 PspOMI G'GGCC_C Cuts at: 2074 2074 Size: 6469 PspXI vC'TCGA_Gb Cuts at: 2645 2645 Size: 6469 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2264 2296 2264 Size: 32 6437 PstI C_TGCA'G Cuts at: 830 2017 2841 3138 3821 830 Size: 1187 824 297 683 3478 Fragments arranged by size: 3478 1187 824 683 297 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6469 PvuII CAG'CTG Cuts at: 2156 2335 2641 2836 3103 2156 Size: 179 306 195 267 5522 Fragments arranged by size: 5522 306 267 195 179 SacI G_AGCT'C Cuts at: 2706 2706 Size: 6469 SalI G'TCGA_C Cuts at: 1936 3811 1936 Size: 1875 4594 SapI GCTCTTCn'nnn_ Cuts at: 2711 2711 Size: 6469 SbfI CC_TGCA'GG Cuts at: 3821 3821 Size: 6469 ScaI AGT'ACT Cuts at: 3560 4717 6429 3560 Size: 1157 1712 3600 Fragments arranged by size: 3600 1712 1157 SexAI A'CCwGG_T Cuts at: 2952 2952 Size: 6469 SfcI C'TryA_G Cuts at: 826 1045 2013 2837 3134 3817 4952 5633 5824 Size: 219 968 824 297 683 1135 681 191 Cuts at: 5824 826 Size: 1471 Fragments arranged by size: 1471 1135 968 824 683 681 297 219 191 SfoI GGC'GCC Cuts at: 2620 2620 Size: 6469 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6469 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6469 SmaI CCC'GGG Cuts at: 1872 2440 2468 3095 3548 3796 1872 Size: 568 28 627 453 248 4545 Fragments arranged by size: 4545 627 568 453 248 28 SmlI C'TyrA_G Cuts at: 820 1017 1943 2600 2645 2843 4573 5474 5736 Size: 197 926 657 45 198 1730 901 262 Cuts at: 5736 820 Size: 1553 Fragments arranged by size: 1730 1553 926 901 657 262 198 197 45 SnaBI TAC'GTA Cuts at: 493 4260 493 Size: 3767 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6469 SphI G_CATG'C Cuts at: 1132 3268 3827 1132 Size: 2136 559 3774 Fragments arranged by size: 3774 2136 559 SspI AAT'ATT Cuts at: 5 52 4393 5 Size: 47 4341 2081 Fragments arranged by size: 4341 2081 47 StuI AGG'CCT Cuts at: 1517 2579 1517 Size: 1062 5407 StyI C'CwwG_G Cuts at: 513 1065 2004 2103 2793 3066 3486 3604 3663 Size: 552 939 99 690 273 420 118 59 Cuts at: 3663 3908 513 Size: 245 3074 Fragments arranged by size: 3074 939 690 552 420 273 245 118 99 59 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2732 3448 3512 4496 4513 4666 4851 Size: 1255 1152 716 64 984 17 153 185 Cuts at: 4851 5296 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1152 984 716 445 185 153 64 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3558 4715 6427 Size: 274 80 33 51 1443 1581 1157 1712 Cuts at: 6427 96 Size: 138 Fragments arranged by size: 1712 1581 1443 1157 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2504 2536 6429 6461 2504 Size: 32 3893 32 2512 Fragments arranged by size: 3893 2512 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6469 XbaI T'CTAG_A Cuts at: 3805 3805 Size: 6469 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2086 2086 Size: 6469 XhoI C'TCGA_G Cuts at: 1943 2645 1943 Size: 702 5767 XmaI C'CCGG_G Cuts at: 1870 2438 2466 3093 3546 3794 1870 Size: 568 28 627 453 248 4545 Fragments arranged by size: 4545 627 568 453 248 28 XmnI GAAnn'nnTTC Cuts at: 3374 3506 4598 3374 Size: 132 1092 5245 Fragments arranged by size: 5245 1092 132 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4277 276 Size: 53 83 186 936 2743 2468 Fragments arranged by size: 2743 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BstXI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI NaeI NgoMIV NruI PacI PmlI RsrII SacII SanDI SfiI SrfI SwaI