(Circular) (Six-base) MAPSORT of: pFN21AE0545.seq Check: 2420 from: 1 to: 5836 pFN21AE0545 5836 bp With 182 enzymes: SgfI * October 20, 2009 10:49 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2074 2074 Size: 5836 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3646 278 Size: 53 83 186 936 2110 2468 Fragments arranged by size: 2468 2110 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2505 3179 1937 Size: 568 674 4594 Fragments arranged by size: 4594 674 568 Acc65I G'GTAC_C Cuts at: 3157 3157 Size: 5836 AclI AA'CG_TT Cuts at: 1563 3963 4336 1563 Size: 2400 373 3063 Fragments arranged by size: 3063 2400 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3899 5283 5551 1632 Size: 2267 1384 268 1917 Fragments arranged by size: 2267 1917 1384 268 AfeI AGC'GCT Cuts at: 2895 2895 Size: 5836 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5639 AflIII A'CryG_T Cuts at: 1203 4735 5712 1203 Size: 3532 977 1327 Fragments arranged by size: 3532 1327 977 AgeI A'CCGG_T Cuts at: 4667 4667 Size: 5836 AhdI GACnn_n'nnGTC Cuts at: 4565 4565 Size: 5836 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5836 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2059 2222 1184 Size: 875 163 4798 Fragments arranged by size: 4798 875 163 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3098 3130 3098 Size: 32 5804 AlwNI CAG_nnn'CTG Cuts at: 3579 5151 3579 Size: 1572 4264 ApaLI G'TGCA_C Cuts at: 2570 3893 5049 2570 Size: 1323 1156 3357 Fragments arranged by size: 3357 1323 1156 ApoI r'AATT_y Cuts at: 1493 3145 3371 3523 5632 1493 Size: 1652 226 152 2109 1697 Fragments arranged by size: 2109 1697 1652 226 152 AseI AT'TA_AT Cuts at: 160 1032 4390 160 Size: 872 3358 1606 Fragments arranged by size: 3358 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5836 AvaI C'yCGr_G Cuts at: 1870 1943 2127 2279 3161 1870 Size: 73 184 152 882 4545 Fragments arranged by size: 4545 882 184 152 73 BamHI G'GATC_C Cuts at: 3166 3533 3166 Size: 367 5469 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2171 2243 3157 4612 618 Size: 325 217 627 384 72 914 1455 1842 Fragments arranged by size: 1842 1455 914 627 384 325 217 72 BanII G_rGCy'C Cuts at: 1391 2126 3155 1391 Size: 735 1029 4072 Fragments arranged by size: 4072 1029 735 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2814 928 Size: 971 915 3950 Fragments arranged by size: 3950 971 915 BbvCI CC'TCA_GC Cuts at: 2615 2615 Size: 5836 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4027 4061 1903 Size: 34 50 34 2006 34 3678 Fragments arranged by size: 3678 2006 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3728 4938 3728 Size: 1210 4626 BclI T'GATC_A Cuts at: 1548 2580 1548 Size: 1032 4804 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2417 4447 136 Size: 107 122 71 631 605 745 2030 1525 Fragments arranged by size: 2030 1525 745 631 605 122 107 71 BglII A'GATC_T Cuts at: 5831 5831 Size: 5836 BlpI GC'TnA_GC Cuts at: 2967 3253 2967 Size: 286 5550 Bme1580I G_kGCm'C Cuts at: 1790 2174 2574 3897 5053 1790 Size: 384 400 1323 1156 2573 Fragments arranged by size: 2573 1323 1156 400 384 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5836 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2668 3567 4525 Size: 416 53 83 399 24 1251 899 958 Cuts at: 4525 442 Size: 1753 Fragments arranged by size: 1753 1251 958 899 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5836 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2450 2803 4496 1239 Size: 1211 353 1693 2579 Fragments arranged by size: 2579 1693 1211 353 Bpu10I CC'TnA_GC Cuts at: 2145 2615 2145 Size: 470 5366 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2477 2735 3961 4826 5124 2477 Size: 258 1226 865 298 3189 Fragments arranged by size: 3189 1226 865 298 258 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2294 4499 882 Size: 617 795 2205 2219 Fragments arranged by size: 2219 2205 795 617 BsaAI yAC'GTr Cuts at: 493 1204 2182 3627 493 Size: 711 978 1445 2702 Fragments arranged by size: 2702 1445 978 711 BsaBI GATnn'nnATC Cuts at: 5830 5830 Size: 5836 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2271 2406 3643 4025 Size: 53 83 186 936 738 135 1237 382 Cuts at: 4025 4660 275 Size: 635 1451 Fragments arranged by size: 1451 1237 936 738 635 382 186 135 83 53 BsaWI w'CCGG_w Cuts at: 4269 4667 4941 5088 5499 4269 Size: 398 274 147 411 4606 Fragments arranged by size: 4606 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3098 3128 1178 Size: 30 165 30 1695 30 3886 Fragments arranged by size: 3886 1695 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2168 2312 3106 1186 Size: 982 144 794 3916 Fragments arranged by size: 3916 982 794 144 BseYI C'CCAG_C Cuts at: 2074 5039 5535 2074 Size: 2965 496 2375 Fragments arranged by size: 2965 2375 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2717 1163 Size: 573 981 4282 Fragments arranged by size: 4282 981 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2560 3286 4047 5075 1540 Size: 275 186 559 726 761 1028 2301 Fragments arranged by size: 2301 1028 761 726 559 275 186 BsiHKAI G_wGCw'C Cuts at: 2469 2574 3897 3982 5053 2469 Size: 105 1323 85 1071 3252 Fragments arranged by size: 3252 1323 1071 105 85 BsmI GAATG_Cn' Cuts at: 3347 3440 3347 Size: 93 5743 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2126 2174 2469 2574 3155 3897 3982 Size: 399 336 48 295 105 581 742 85 Cuts at: 3982 5053 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 581 399 336 295 105 85 48 BspEI T'CCGG_A Cuts at: 5499 5499 Size: 5836 BspHI T'CATG_A Cuts at: 3725 5455 3725 Size: 1730 4106 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2074 3191 844 Size: 1230 1117 3489 Fragments arranged by size: 3489 1230 1117 BsrBI CCG'CTC Cuts at: 1394 1823 2212 2854 3723 1394 Size: 429 389 642 869 3507 Fragments arranged by size: 3507 869 642 429 389 BsrDI GCAATG_nn' Cuts at: 65 1248 2598 4331 4505 65 Size: 1183 1350 1733 174 1396 Fragments arranged by size: 1733 1396 1350 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2039 2455 4480 4667 1700 Size: 110 229 416 2025 187 2869 Fragments arranged by size: 2869 2025 416 229 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5836 BssHII G'CGCG_C Cuts at: 1925 2257 1925 Size: 332 5504 BssSI C'ACGA_G Cuts at: 3896 4908 3896 Size: 1012 4824 BstAPI GCAn_nnn'nTGC Cuts at: 794 2728 794 Size: 1934 3902 BstBI TT'CG_AA Cuts at: 4640 4640 Size: 5836 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5836 BstXI CCAn_nnnn'nTGG Cuts at: 2182 2921 2182 Size: 739 5097 BstYI r'GATC_y Cuts at: 1972 3166 3533 3918 3935 5387 5473 5485 5831 Size: 1194 367 385 17 1452 86 12 346 Cuts at: 5831 1972 Size: 1977 Fragments arranged by size: 1977 1452 1194 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2859 513 Size: 552 1794 3490 Fragments arranged by size: 3490 1794 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2761 487 Size: 683 56 1535 3562 Fragments arranged by size: 3562 1535 683 56 BtsI GCAGTG_nn' Cuts at: 2245 3352 4146 4166 2245 Size: 1107 794 20 3915 Fragments arranged by size: 3915 1107 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5836 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2809 2844 529 Size: 35 2245 35 3521 Fragments arranged by size: 3521 2245 35 35 DraI TTT'AAA Cuts at: 3140 3140 Size: 5836 DraIII CAC_nnn'GTG Cuts at: 2643 2643 Size: 5836 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3023 4843 809 Size: 507 1707 1820 1802 Fragments arranged by size: 1820 1802 1707 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2325 2353 2557 Size: 54 1425 50 275 18 495 28 204 Cuts at: 2557 2911 3283 4172 5801 8 Size: 354 372 889 1629 43 Fragments arranged by size: 1629 1425 889 495 372 354 275 204 54 50 43 28 18 EagI C'GGCC_G Cuts at: 1537 1812 2557 3283 1537 Size: 275 745 726 4090 Fragments arranged by size: 4090 745 726 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3766 1353 Size: 2413 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4423 4797 4943 387 Size: 1064 138 2834 374 146 1280 Fragments arranged by size: 2834 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2450 2803 3899 4496 5283 5551 1239 Size: 393 818 353 1096 597 787 268 1524 Fragments arranged by size: 1524 1096 818 787 597 393 353 268 EcoO109I rG'GnC_Cy Cuts at: 2479 2479 Size: 5836 EcoRI G'AATT_C Cuts at: 3145 3523 3145 Size: 378 5458 FspI TGC'GCA Cuts at: 4342 4342 Size: 5836 HaeII r_GCGC'y Cuts at: 1678 2746 2897 4983 1678 Size: 1068 151 2086 2531 Fragments arranged by size: 2531 2086 1068 151 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4477 4509 4551 4583 1373 Size: 32 135 32 2905 32 42 32 2626 Fragments arranged by size: 2905 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2884 3180 3426 669 Size: 988 281 946 296 246 3079 Fragments arranged by size: 3079 988 946 296 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5836 HpaI GTT'AAC Cuts at: 2884 3426 2884 Size: 542 5294 KpnI G_GTAC'C Cuts at: 3161 3161 Size: 5836 MfeI C'AATT_G Cuts at: 3435 3435 Size: 5836 MluI A'CGCG_T Cuts at: 5712 5712 Size: 5836 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4950 5134 1305 Size: 3645 184 2007 Fragments arranged by size: 3645 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2327 2913 10 Size: 54 1425 343 495 586 2933 Fragments arranged by size: 2933 1425 586 495 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2219 2252 2660 3794 4153 4312 518 Size: 648 1053 33 408 1134 359 159 2042 Fragments arranged by size: 2042 1134 1053 648 408 359 159 33 MspA1I CmG'CkG Cuts at: 1606 2074 2725 3043 3252 3929 5077 5322 1606 Size: 468 651 318 209 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 651 468 318 245 209 NaeI GCC'GGC Cuts at: 2041 2457 2041 Size: 416 5420 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5284 NdeI CA'TA_TG Cuts at: 387 387 Size: 5836 NgoMIV G'CCGG_C Cuts at: 2039 2455 2039 Size: 416 5420 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5836 NotI GC'GGCC_GC Cuts at: 3283 3283 Size: 5836 NspI r_CATG'y Cuts at: 1132 1229 3194 4739 1132 Size: 97 1965 1545 2229 Fragments arranged by size: 2229 1965 1545 97 PasI CC'CwG_GG Cuts at: 1348 2921 1348 Size: 1573 4263 PciI A'CATG_T Cuts at: 4735 4735 Size: 5836 PflMI CCAn_nnn'nTGG Cuts at: 1108 2680 1108 Size: 1572 4264 PfoI T'CCnGG_A Cuts at: 2427 2427 Size: 5836 PmeI GTTT'AAAC Cuts at: 3140 3140 Size: 5836 PmlI CAC'GTG Cuts at: 2182 2182 Size: 5836 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3098 3130 3905 3937 5418 5450 3098 Size: 32 775 32 1481 32 3484 Fragments arranged by size: 3484 1481 775 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2479 2479 Size: 5836 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5836 PsiI TTA'TAA Cuts at: 3406 3406 Size: 5836 PstI C_TGCA'G Cuts at: 830 3188 830 Size: 2358 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5836 PvuII CAG'CTG Cuts at: 2074 2725 2074 Size: 651 5185 SalI G'TCGA_C Cuts at: 1936 3178 1936 Size: 1242 4594 SbfI CC_TGCA'GG Cuts at: 3188 3188 Size: 5836 ScaI AGT'ACT Cuts at: 4084 5796 4084 Size: 1712 4124 SexAI A'CCwGG_T Cuts at: 2865 2865 Size: 5836 SfcI C'TryA_G Cuts at: 826 1045 3184 4319 5000 5191 826 Size: 219 2139 1135 681 191 1471 Fragments arranged by size: 2139 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5836 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5836 SmaI CCC'GGG Cuts at: 1872 2129 3163 1872 Size: 257 1034 4545 Fragments arranged by size: 4545 1034 257 SmlI C'TyrA_G Cuts at: 820 1017 1943 2492 2750 3940 4841 5103 820 Size: 197 926 549 258 1190 901 262 1553 Fragments arranged by size: 1553 1190 926 901 549 262 258 197 SnaBI TAC'GTA Cuts at: 493 3627 493 Size: 3134 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5836 SphI G_CATG'C Cuts at: 1132 3194 1132 Size: 2062 3774 SspI AAT'ATT Cuts at: 5 52 3760 5 Size: 47 3708 2081 Fragments arranged by size: 3708 2081 47 StuI AGG'CCT Cuts at: 1517 2339 2420 1517 Size: 822 81 4933 Fragments arranged by size: 4933 822 81 StyI C'CwwG_G Cuts at: 513 1065 3275 513 Size: 552 2210 3074 Fragments arranged by size: 3074 2210 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2213 2491 3863 3880 4033 4218 4663 Size: 1255 633 278 1372 17 153 185 445 Cuts at: 4663 325 Size: 1498 Fragments arranged by size: 1498 1372 1255 633 445 278 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2712 4082 5794 Size: 274 80 33 51 1443 735 1370 1712 Cuts at: 5794 96 Size: 138 Fragments arranged by size: 1712 1443 1370 735 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2070 2102 5796 5828 2070 Size: 32 3694 32 2078 Fragments arranged by size: 3694 2078 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2792 2867 1316 Size: 1476 75 4285 Fragments arranged by size: 4285 1476 75 XbaI T'CTAG_A Cuts at: 3172 3172 Size: 5836 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5836 XmaI C'CCGG_G Cuts at: 1870 2127 3161 1870 Size: 257 1034 4545 Fragments arranged by size: 4545 1034 257 XmnI GAAnn'nnTTC Cuts at: 3965 3965 Size: 5836 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3644 276 Size: 53 83 186 936 2110 2468 Fragments arranged by size: 2468 2110 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI