Restriction map for
pFN21AE0545
(Linear) (Six-base) MAPSORT of: KIEE0545.seq Check: 4487 from: 1 to: 1131
KIEE0545.orf
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 69 1131
Size: 69 1062
AccI GT'mk_AC
Cuts at: 0 500 1131
Size: 500 631
AfeI AGC'GCT
Cuts at: 0 890 1131
Size: 890 241
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 54 217 1131
Size: 54 163 914
Fragments arranged by size:
914 163 54
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1093 1125 1131
Size: 1093 32 6
Fragments arranged by size:
1093 32 6
ApaLI G'TGCA_C
Cuts at: 0 565 1131
Size: 565 566
AvaI C'yCGr_G
Cuts at: 0 122 274 1131
Size: 122 152 857
Fragments arranged by size:
857 152 122
BanI G'GyrC_C
Cuts at: 0 166 238 1131
Size: 166 72 893
Fragments arranged by size:
893 166 72
BanII G_rGCy'C
Cuts at: 0 121 1131
Size: 121 1010
BbsI GAAGACnn'nnnn_
Cuts at: 0 809 1131
Size: 809 322
BbvCI CC'TCA_GC
Cuts at: 0 610 1131
Size: 610 521
BclI T'GATC_A
Cuts at: 0 575 1131
Size: 575 556
BglI GCCn_nnn'nGGC
Cuts at: 0 412 1131
Size: 412 719
BlpI GC'TnA_GC
Cuts at: 0 962 1131
Size: 962 169
Bme1580I G_kGCm'C
Cuts at: 0 169 569 1131
Size: 169 400 562
Fragments arranged by size:
562 400 169
BmrI ACTGGGnnnn_n'
Cuts at: 0 663 1131
Size: 663 468
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 445 798 1131
Size: 445 353 333
Fragments arranged by size:
445 353 333
Bpu10I CC'TnA_GC
Cuts at: 0 140 610 1131
Size: 140 470 521
Fragments arranged by size:
521 470 140
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 472 730 1131
Size: 472 258 401
Fragments arranged by size:
472 401 258
BsaI GGTCTCn'nnnn_
Cuts at: 0 289 1131
Size: 289 842
BsaAI yAC'GTr
Cuts at: 0 177 1131
Size: 177 954
BsaHI Gr'CG_yC
Cuts at: 0 266 401 1131
Size: 266 135 730
Fragments arranged by size:
730 266 135
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1093 1123 1131
Size: 1093 30 8
Fragments arranged by size:
1093 30 8
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 163 307 1101 1131
Size: 163 144 794 30
Fragments arranged by size:
794 163 144 30
BseYI C'CCAG_C
Cuts at: 0 69 1131
Size: 69 1062
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 712 1131
Size: 712 419
BsiEI CG_ry'CG
Cuts at: 0 555 1131
Size: 555 576
BsiHKAI G_wGCw'C
Cuts at: 0 464 569 1131
Size: 464 105 562
Fragments arranged by size:
562 464 105
Bsp1286I G_dGCh'C
Cuts at: 0 121 169 464 569 1131
Size: 121 48 295 105 562
Fragments arranged by size:
562 295 121 105 48
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 69 1131
Size: 69 1062
BsrBI CCG'CTC
Cuts at: 0 207 849 1131
Size: 207 642 282
Fragments arranged by size:
642 282 207
BsrDI GCAATG_nn'
Cuts at: 0 593 1131
Size: 593 538
BsrFI r'CCGG_y
Cuts at: 0 34 450 1131
Size: 34 416 681
Fragments arranged by size:
681 416 34
BssHII G'CGCG_C
Cuts at: 0 252 1131
Size: 252 879
BstAPI GCAn_nnn'nTGC
Cuts at: 0 723 1131
Size: 723 408
BstXI CCAn_nnnn'nTGG
Cuts at: 0 177 916 1131
Size: 177 739 215
Fragments arranged by size:
739 215 177
BtgI C'CryG_G
Cuts at: 0 854 1131
Size: 854 277
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 756 1131
Size: 756 375
BtsI GCAGTG_nn'
Cuts at: 0 240 1131
Size: 240 891
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 804 839 1131
Size: 804 35 292
Fragments arranged by size:
804 292 35
DraIII CAC_nnn'GTG
Cuts at: 0 638 1131
Size: 638 493
DrdI GACnn_nn'nnGTC
Cuts at: 0 1018 1131
Size: 1018 113
EaeI y'GGCC_r
Cuts at: 0 320 348 552 906 1131
Size: 320 28 204 354 225
Fragments arranged by size:
354 320 225 204 28
EagI C'GGCC_G
Cuts at: 0 552 1131
Size: 552 579
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 445 798 1131
Size: 445 353 333
Fragments arranged by size:
445 353 333
EcoO109I rG'GnC_Cy
Cuts at: 0 474 1131
Size: 474 657
HaeII r_GCGC'y
Cuts at: 0 741 892 1131
Size: 741 151 239
Fragments arranged by size:
741 239 151
HincII GTy'rAC
Cuts at: 0 879 1131
Size: 879 252
HpaI GTT'AAC
Cuts at: 0 879 1131
Size: 879 252
MscI TGG'CCA
Cuts at: 0 322 908 1131
Size: 322 586 223
Fragments arranged by size:
586 322 223
MslI CAynn'nnrTG
Cuts at: 0 214 247 655 1131
Size: 214 33 408 476
Fragments arranged by size:
476 408 214 33
MspA1I CmG'CkG
Cuts at: 0 69 720 1038 1131
Size: 69 651 318 93
Fragments arranged by size:
651 318 93 69
NaeI GCC'GGC
Cuts at: 0 36 452 1131
Size: 36 416 679
Fragments arranged by size:
679 416 36
NgoMIV G'CCGG_C
Cuts at: 0 34 450 1131
Size: 34 416 681
Fragments arranged by size:
681 416 34
PasI CC'CwG_GG
Cuts at: 0 916 1131
Size: 916 215
PflMI CCAn_nnn'nTGG
Cuts at: 0 675 1131
Size: 675 456
PfoI T'CCnGG_A
Cuts at: 0 422 1131
Size: 422 709
PmlI CAC'GTG
Cuts at: 0 177 1131
Size: 177 954
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1093 1125 1131
Size: 1093 32 6
Fragments arranged by size:
1093 32 6
PpuMI rG'GwC_Cy
Cuts at: 0 474 1131
Size: 474 657
PvuII CAG'CTG
Cuts at: 0 69 720 1131
Size: 69 651 411
Fragments arranged by size:
651 411 69
SexAI A'CCwGG_T
Cuts at: 0 860 1131
Size: 860 271
SmaI CCC'GGG
Cuts at: 0 124 1131
Size: 124 1007
SmlI C'TyrA_G
Cuts at: 0 487 745 1131
Size: 487 258 386
Fragments arranged by size:
487 386 258
StuI AGG'CCT
Cuts at: 0 334 415 1131
Size: 334 81 716
Fragments arranged by size:
716 334 81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 208 486 1131
Size: 208 278 645
Fragments arranged by size:
645 278 208
TatI w'GTAC_w
Cuts at: 0 707 1131
Size: 707 424
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 65 97 1131
Size: 65 32 1034
Fragments arranged by size:
1034 65 32
Tth111I GACn'n_nGTC
Cuts at: 0 787 862 1131
Size: 787 75 269
Fragments arranged by size:
787 269 75
XmaI C'CCGG_G
Cuts at: 0 122 1131
Size: 122 1009
Enzymes that do cut:
AarI AccI AfeI AlfI AloI ApaLI AvaI BanI
BanII BbsI BbvCI BclI BglI BlpI Bme1580I BmrI
BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrDI
BsrFI BssHII BstAPI BstXI BtgI BtgZI BtsI CspCI
DraIII DrdI EaeI EagI Eco57MI EcoO109I HaeII HincII
HpaI MscI MslI MspA1I NaeI NgoMIV PasI PflMI
PfoI PmlI PpiI PpuMI PvuII SexAI SmaI SmlI
StuI TaqII TatI TstI Tth111I XmaI
Enzymes that do not cut:
AatII Acc65I AclI AcuI AflII AflIII AgeI AhdI
AleI AlwNI ApaI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BcgI BciVI BfrBI BglII BmgBI
BmtI BplI BsaBI BsaWI BsiWI BsmI BsmBI BspEI
BspHI BsrGI BssSI BstBI BstEII BstYI BstZ17I Bsu36I
ClaI DraI EarI EciI EcoICRI EcoNI EcoRI EcoRV
FalI FseI FspI FspAI Hin4I HindIII KasI KpnI
MfeI MluI MmeI NarI NcoI NdeI NheI NotI
NruI NsiI NspI PacI PciI PmeI PshAI PsiI
PspOMI PspXI PsrI PstI PvuI RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI
SgfI SgrAI SnaBI SpeI SphI SrfI SspI StyI
SwaI XbaI XcmI XhoI XmnI ZraI