(Circular) (Six-base) MAPSORT of: pFN21AE0451.seq Check: 3370 from: 1 to: 5629 pFN21AE0451 5629 bp With 182 enzymes: SgfI * October 20, 2009 10:47 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3439 278 Size: 53 83 186 936 1903 2468 Fragments arranged by size: 2468 1903 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2972 1937 Size: 1035 4594 Acc65I G'GTAC_C Cuts at: 2950 2950 Size: 5629 AclI AA'CG_TT Cuts at: 1563 3756 4129 1563 Size: 2193 373 3063 Fragments arranged by size: 3063 2193 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2896 3692 5076 5344 1632 Size: 1264 796 1384 268 1917 Fragments arranged by size: 1917 1384 1264 796 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5432 AflIII A'CryG_T Cuts at: 1203 4528 5505 1203 Size: 3325 977 1327 Fragments arranged by size: 3325 1327 977 AgeI A'CCGG_T Cuts at: 4460 4460 Size: 5629 AhdI GACnn_n'nnGTC Cuts at: 4358 4358 Size: 5629 AleI CACnn'nnGTG Cuts at: 1166 2208 2279 1166 Size: 1042 71 4516 Fragments arranged by size: 4516 1042 71 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5629 AlwNI CAG_nnn'CTG Cuts at: 3372 4944 3372 Size: 1572 4057 ApaI G_GGCC'C Cuts at: 2656 2656 Size: 5629 ApaLI G'TGCA_C Cuts at: 3686 4842 3686 Size: 1156 4473 ApoI r'AATT_y Cuts at: 1493 2938 3164 3316 5425 1493 Size: 1445 226 152 2109 1697 Fragments arranged by size: 2109 1697 1445 226 152 AseI AT'TA_AT Cuts at: 160 1032 4183 160 Size: 872 3151 1606 Fragments arranged by size: 3151 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5629 AvaI C'yCGr_G Cuts at: 1870 1943 2954 1870 Size: 73 1011 4545 Fragments arranged by size: 4545 1011 73 AvrII C'CTAG_G Cuts at: 2458 2458 Size: 5629 BamHI G'GATC_C Cuts at: 2959 3326 2959 Size: 367 5262 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2026 2950 4405 618 Size: 325 217 627 239 924 1455 1842 Fragments arranged by size: 1842 1455 924 627 325 239 217 BanII G_rGCy'C Cuts at: 1391 2057 2656 2948 1391 Size: 666 599 292 4072 Fragments arranged by size: 4072 666 599 292 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2368 928 Size: 971 469 4189 Fragments arranged by size: 4189 971 469 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3820 3854 1903 Size: 34 1883 34 3678 Fragments arranged by size: 3678 1883 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3521 4731 3521 Size: 1210 4419 BclI T'GATC_A Cuts at: 1548 2490 1548 Size: 942 4687 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4240 136 Size: 107 122 71 631 605 2568 1525 Fragments arranged by size: 2568 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5624 5624 Size: 5629 BlpI GC'TnA_GC Cuts at: 3046 3046 Size: 5629 Bme1580I G_kGCm'C Cuts at: 1790 2215 2656 3690 4846 1790 Size: 425 441 1034 1156 2573 Fragments arranged by size: 2573 1156 1034 441 425 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5629 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2629 3360 4318 Size: 416 53 83 399 24 1212 731 958 Cuts at: 4318 442 Size: 1753 Fragments arranged by size: 1753 1212 958 731 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5629 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2168 2538 2894 2168 Size: 370 356 4903 Fragments arranged by size: 4903 370 356 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2849 4289 1239 Size: 1610 1440 2579 Fragments arranged by size: 2579 1610 1440 Bpu10I CC'TnA_GC Cuts at: 2367 2367 Size: 5629 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3754 4619 4917 3754 Size: 865 298 4466 Fragments arranged by size: 4466 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4292 882 Size: 617 2793 2219 Fragments arranged by size: 2793 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3420 493 Size: 711 2216 2702 Fragments arranged by size: 2702 2216 711 BsaBI GATnn'nnATC Cuts at: 5623 5623 Size: 5629 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3436 3818 4453 275 Size: 53 83 186 936 1903 382 635 1451 Fragments arranged by size: 1903 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4062 4460 4734 4881 5292 4062 Size: 398 274 147 411 4399 Fragments arranged by size: 4399 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2535 2565 1178 Size: 30 165 30 1132 30 4242 Fragments arranged by size: 4242 1132 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2333 2556 1186 Size: 1147 223 4259 Fragments arranged by size: 4259 1147 223 BseYI C'CCAG_C Cuts at: 2128 2536 2597 4832 5328 2128 Size: 408 61 2235 496 2429 Fragments arranged by size: 2429 2235 496 408 61 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2087 1163 Size: 573 351 4705 Fragments arranged by size: 4705 573 351 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3079 3840 4868 1540 Size: 275 186 1078 761 1028 2301 Fragments arranged by size: 2301 1078 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3690 3775 4846 3690 Size: 85 1071 4473 Fragments arranged by size: 4473 1071 85 BsmI GAATG_Cn' Cuts at: 3140 3233 3140 Size: 93 5536 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2057 2215 2656 2948 3690 3775 4846 Size: 399 267 158 441 292 742 85 1071 Cuts at: 4846 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 441 399 292 267 158 85 BspEI T'CCGG_A Cuts at: 5292 5292 Size: 5629 BspHI T'CATG_A Cuts at: 3518 5248 3518 Size: 1730 3899 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2984 844 Size: 2140 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2356 3516 1394 Size: 429 533 1160 3507 Fragments arranged by size: 3507 1160 533 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4124 4298 65 Size: 1183 2876 174 1396 Fragments arranged by size: 2876 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2559 4273 4460 1700 Size: 110 749 1714 187 2869 Fragments arranged by size: 2869 1714 749 187 110 BsrGI T'GTAC_A Cuts at: 96 2614 96 Size: 2518 3111 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5629 BssSI C'ACGA_G Cuts at: 3689 4701 3689 Size: 1012 4617 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5629 BstBI TT'CG_AA Cuts at: 4433 4433 Size: 5629 BstEII G'GTnAC_C Cuts at: 1184 2750 1184 Size: 1566 4063 BstYI r'GATC_y Cuts at: 1972 2959 3326 3711 3728 5180 5266 5278 5624 Size: 987 367 385 17 1452 86 12 346 Cuts at: 5624 1972 Size: 1977 Fragments arranged by size: 1977 1452 987 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4138 Fragments arranged by size: 4138 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4890 Fragments arranged by size: 4890 683 56 BtsI GCAGTG_nn' Cuts at: 2320 3145 3939 3959 2320 Size: 825 794 20 3990 Fragments arranged by size: 3990 825 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5629 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5594 DraI TTT'AAA Cuts at: 2933 2933 Size: 5629 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4636 809 Size: 507 3320 1802 Fragments arranged by size: 3320 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2564 3076 3965 Size: 54 1425 50 275 18 734 512 889 Cuts at: 3965 5594 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 734 512 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3076 1537 Size: 275 1264 4090 Fragments arranged by size: 4090 1264 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2568 3559 1353 Size: 1215 991 3423 Fragments arranged by size: 3423 1215 991 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4216 4590 4736 387 Size: 1064 138 2627 374 146 1280 Fragments arranged by size: 2627 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2849 2896 3692 4289 5076 5344 1239 Size: 393 1217 47 796 597 787 268 1524 Fragments arranged by size: 1524 1217 796 787 597 393 268 47 EcoO109I rG'GnC_Cy Cuts at: 2652 2652 Size: 5629 EcoRI G'AATT_C Cuts at: 2938 3316 2938 Size: 378 5251 EcoRV GAT'ATC Cuts at: 2231 2231 Size: 5629 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2392 2392 Size: 5629 FspI TGC'GCA Cuts at: 4135 4135 Size: 5629 HaeII r_GCGC'y Cuts at: 1678 4776 1678 Size: 3098 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2220 2252 2830 2862 4270 Size: 32 135 32 648 32 578 32 1408 Cuts at: 4270 4302 4344 4376 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1408 648 578 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2161 2973 3219 669 Size: 988 281 223 812 246 3079 Fragments arranged by size: 3079 988 812 281 246 223 HindIII A'AGCT_T Cuts at: 748 748 Size: 5629 HpaI GTT'AAC Cuts at: 3219 3219 Size: 5629 KpnI G_GTAC'C Cuts at: 2954 2954 Size: 5629 MfeI C'AATT_G Cuts at: 3228 3228 Size: 5629 MluI A'CGCG_T Cuts at: 5505 5505 Size: 5629 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2677 4743 4927 1305 Size: 1372 2066 184 2007 Fragments arranged by size: 2066 2007 1372 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3807 Fragments arranged by size: 3807 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2208 2238 2279 2921 3587 3946 4105 Size: 648 1042 30 41 642 666 359 159 Cuts at: 4105 518 Size: 2042 Fragments arranged by size: 2042 1042 666 648 642 359 159 41 30 MspA1I CmG'CkG Cuts at: 1606 3045 3722 4870 5115 1606 Size: 1439 677 1148 245 2120 Fragments arranged by size: 2120 1439 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4138 Fragments arranged by size: 4138 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5629 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5629 NotI GC'GGCC_GC Cuts at: 3076 3076 Size: 5629 NspI r_CATG'y Cuts at: 1132 1229 2987 4532 1132 Size: 97 1758 1545 2229 Fragments arranged by size: 2229 1758 1545 97 PasI CC'CwG_GG Cuts at: 1348 2084 2215 1348 Size: 736 131 4762 Fragments arranged by size: 4762 736 131 PciI A'CATG_T Cuts at: 4528 4528 Size: 5629 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5629 PmeI GTTT'AAAC Cuts at: 2933 2933 Size: 5629 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3698 3730 5211 5243 3698 Size: 32 1481 32 4084 Fragments arranged by size: 4084 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5629 PsiI TTA'TAA Cuts at: 3199 3199 Size: 5629 PspOMI G'GGCC_C Cuts at: 2652 2652 Size: 5629 PstI C_TGCA'G Cuts at: 830 2514 2981 830 Size: 1684 467 3478 Fragments arranged by size: 3478 1684 467 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5629 SalI G'TCGA_C Cuts at: 1936 2971 1936 Size: 1035 4594 SbfI CC_TGCA'GG Cuts at: 2981 2981 Size: 5629 ScaI AGT'ACT Cuts at: 2606 3877 5589 2606 Size: 1271 1712 2646 Fragments arranged by size: 2646 1712 1271 SfcI C'TryA_G Cuts at: 826 1045 2510 2977 4112 4793 4984 826 Size: 219 1465 467 1135 681 191 1471 Fragments arranged by size: 1471 1465 1135 681 467 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5629 SgrAI Cr'CCGG_yG Cuts at: 1700 2559 1700 Size: 859 4770 SmaI CCC'GGG Cuts at: 1872 2956 1872 Size: 1084 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3733 4634 4896 820 Size: 197 926 1790 901 262 1553 Fragments arranged by size: 1790 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3420 493 Size: 2927 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5629 SphI G_CATG'C Cuts at: 1132 2987 1132 Size: 1855 3774 SspI AAT'ATT Cuts at: 5 52 3553 5 Size: 47 3501 2081 Fragments arranged by size: 3501 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5629 StyI C'CwwG_G Cuts at: 513 1065 2004 2458 2866 3068 513 Size: 552 939 454 408 202 3074 Fragments arranged by size: 3074 939 552 454 408 202 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3656 3673 3826 4011 4456 325 Size: 1255 2076 17 153 185 445 1498 Fragments arranged by size: 2076 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2604 2614 3875 Size: 274 80 33 51 1443 627 10 1261 Cuts at: 3875 5587 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1261 627 274 138 80 51 33 10 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5589 5621 5589 Size: 32 5597 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5629 XbaI T'CTAG_A Cuts at: 2965 2965 Size: 5629 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2590 2590 Size: 5629 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5629 XmaI C'CCGG_G Cuts at: 1870 2954 1870 Size: 1084 4545 XmnI GAAnn'nnTTC Cuts at: 2688 3758 2688 Size: 1070 4559 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3437 276 Size: 53 83 186 936 1903 2468 Fragments arranged by size: 2468 1903 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI