################################## # NO CUT ENZYMES ( 169 enzymes) # ################################## AatII Acc65I AccI AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AvaI AviII AvrII BalI BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsiHKAI BsiWI BsoBI BspDI BspHI BspLU11I BspMI BsrBI BsrDI BssHII BssSI Bst1107I BstBI BstEII BstF5I BstPI BstXI BstYI BstZ17I Cac8I CelII CfoI ClaI DraI DraIII DrdI Eam1105I EarI Eco47III EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FokI FseI FspI HaeII HhaI Hin1I HinP1I HincII HindII HindIII HinfI HpaI HphI KasI KpnI Ksp632I MaeII MaeIII MamI MfeI MflI MluI MluNI MscI MseI MslI MunI NaeI NarI NcoI NdeI NgoMIV NheI NlaIII NotI NruI NsiI NspI NspV PacI PaeR7I PflMI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI SacI SalI SapI SbfI ScaI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI StuI StyI SwaI TaqI TfiI Tru9I Tsp45I TspRI TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AccIII 165 TCCGGA T^CCGGA AfaI 231 GTAC GT^AC AlwI 279 GATCC GGATC(4/5) AlwNI 334 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG AocI 82 CCTGAGG CC^T[ACGT]AGG AspI 126 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC BcnI 45 CCCGG CC^[GC]GG BpmI 94 CTGGAG CTGGAG(16/14) BsaI 124 GAGACC GGTCTC(1/5) BseAI 165 TCCGGA T^CCGGA BsgI 26 GTGCAG GTGCAG(16/14) BsiEI 185 CGGCCG CG[GA][CT]^CG BsmBI 304 GAGACG CGTCTC(1/5) BsmI 389 GAATGC GAATGC(1/-1) Bsp1407I 230 TGTACA T^GTACA BspEI 165 TCCGGA T^CCGGA BsrFI 22 GCCGGT [GA]^CCGG[CT] BsrGI 230 TGTACA T^GTACA BsrI 39 CCAGT ACTGG(1/-1) BstAPI 333 GCAGCAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Bsu36I 82 CCTGAGG CC^T[ACGT]AGG Cfr10I 22 GCCGGT [GA]^CCGG[CT] CpoI 178 CGGTCCG CG^G[AT]CCG DsaI 182 CCGCGG C^C[GA][CT]GG EaeI 185 CGGCCG [CT]^GGCC[GA] EagI 185 CGGCCG C^GGCCG EclXI 185 CGGCCG C^GGCCG Eco52I 185 CGGCCG C^GGCCG Eco81I 82 CCTGAGG CC^T[ACGT]AGG EcoNI 367 CCTAACACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG HgaI 70 GACGC GACGC(5/10) KspI 182 CCGCGG CCGC^GG MboII 288 TCTTC GAAGA(8/7) MroI 165 TCCGGA T^CCGGA NciI 45 CCCGG CC^[GC]GG PflFI 126 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 363 AGGACCT [GA]G^G[AT]CC[CT] PstI 340 CTGCAG CTGCA^G RsaI 231 GTAC GT^AC RsrII 178 CGGTCCG CG^G[AT]CCG SacII 182 CCGCGG CCGC^GG SfcI 340 CTGCAG C^T[GA][CT]AG SspBI 230 TGTACA T^GTACA Tsp509I 53 AATT ^AATT Tth111I 126 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### AccII 72 CGCG CG^CG 183 CGCG AluI 92 AGCT AG^CT 116 AGCT BanII 376 GGGCTC G[GA]GC[CT]^C 398 GGGCTC BfaI 118 CTAG C^TAG 361 CTAG BmyI 376 GGGCTC G[AGT]GC[ACT]^C 398 GGGCTC BsaWI 165 TCCGGA [AT]^CCGG[AT] 176 TCCGGT BsmFI 262 GGGAC GGGAC(10/14) 42 GTCCC Bsp1286I 376 GGGCTC G[AGT]GC[ACT]^C 398 GGGCTC BstUI 72 CGCG CG^CG 183 CGCG DraII 146 GGGGCCT [GA]G^G[ACGT]CC[CT] 363 AGGACCT EcoO109I 146 GGGGCCT [GA]G^G[ACGT]CC[CT] 363 AGGACCT HaeIII 148 GGCC GG^CC 186 GGCC MaeI 118 CTAG C^TAG 361 CTAG MspA1I 182 CCGCGG C[AC]G^C[GT]G 346 CAGCGG MvnI 72 CGCG CG^CG 183 CGCG ######################################### # 3 OR MORE CUT ENZYMES ( 29 enzymes) # ######################################### AciI AvaII BbvI BsaJI BseRI BsiYI BslI BsmAI BssKI BstNI Cfr13I DdeI DpnII EcoRII Fnu4HI HapII HpaII ItaI MboI MnlI MspI MvaI MwoI NdeII NlaIV Sau3AI Sau96I ScrFI TseI