(Linear) (Six-base) MAPSORT of: KIEE6128.seq  Check: 1453  from: 1  to: 426



KIEE6128.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:25  ..

AlwNI CAG_nnn'CTG

Cuts at:      0     339     426 
   Size:        339      87
 
BanII G_rGCy'C

Cuts at:      0     380     402     426 
   Size:        380      22      24

  Fragments arranged by size: 

                 380      24      22
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      33     426 
   Size:         33     393
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     115     426 
   Size:        115     311
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     118     426 
   Size:        118     308
 
BsaWI w'CCGG_w

Cuts at:      0     165     176     426 
   Size:        165      11     250

  Fragments arranged by size: 

                 250     165      11
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     100     103     112     127     426 
   Size:        100       3       9      15     299

  Fragments arranged by size: 

                 299     100      15       9       3
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      47     426 
   Size:         47     379
 
BsiEI CG_ry'CG

Cuts at:      0     188     426 
   Size:        188     238
 
BsmI GAATG_Cn'

Cuts at:      0     395     426 
   Size:        395      31
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     298     426 
   Size:        298     128
 
Bsp1286I G_dGCh'C

Cuts at:      0     380     402     426 
   Size:        380      22      24

  Fragments arranged by size: 

                 380      24      22
 
BspEI T'CCGG_A

Cuts at:      0     165     426 
   Size:        165     261
 
BsrFI r'CCGG_y

Cuts at:      0      22     426 
   Size:         22     404
 
BsrGI T'GTAC_A

Cuts at:      0     230     426 
   Size:        230     196
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     339     426 
   Size:        339      87
 
Bsu36I CC'TnA_GG

Cuts at:      0      83     426 
   Size:         83     343
 
BtgI C'CryG_G

Cuts at:      0     182     426 
   Size:        182     244
 
EaeI y'GGCC_r

Cuts at:      0     185     426 
   Size:        185     241
 
EagI C'GGCC_G

Cuts at:      0     185     426 
   Size:        185     241
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     115     426 
   Size:        115     311
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     371     426 
   Size:        371      55
 
EcoO109I rG'GnC_Cy

Cuts at:      0     147     364     426 
   Size:        147     217      62

  Fragments arranged by size: 

                 217     147      62
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     115     147     426 
   Size:        115      32     279

  Fragments arranged by size: 

                 279     115      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     113     426 
   Size:        113     313
 
MspA1I CmG'CkG

Cuts at:      0     184     348     426 
   Size:        184     164      78

  Fragments arranged by size: 

                 184     164      78
 
PfoI T'CCnGG_A

Cuts at:      0     414     426 
   Size:        414      12
 
PpuMI rG'GwC_Cy

Cuts at:      0     364     426 
   Size:        364      62
 
PstI C_TGCA'G

Cuts at:      0     344     426 
   Size:        344      82
 
RsrII CG'GwC_CG

Cuts at:      0     179     426 
   Size:        179     247
 
SacII CC_GC'GG

Cuts at:      0     185     426 
   Size:        185     241
 
SfcI C'TryA_G

Cuts at:      0     340     426 
   Size:        340      86
 
TatI w'GTAC_w

Cuts at:      0     230     426 
   Size:        230     196
 
Tth111I GACn'n_nGTC

Cuts at:      0     129     426 
   Size:        129     297
 

 Enzymes that do cut:

    AlwNI    BanII     BmrI     BpmI     BsaI    BsaWI    BseRI     BsgI
    BsiEI     BsmI    BsmBI Bsp1286I    BspEI    BsrFI    BsrGI   BstAPI
   Bsu36I     BtgI     EaeI     EagI  Eco57MI    EcoNI EcoO109I    Hin4I
     MmeI   MspA1I     PfoI    PpuMI     PstI    RsrII    SacII     SfcI
     TatI  Tth111I

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmtI     BplI   Bpu10I
    BpuEI    BsaAI    BsaBI    BsaHI    BsaXI    BseYI  BsiHKAI    BsiWI
    BspHI    BspMI    BsrBI    BsrDI   BssHII    BssSI    BstBI   BstEII
    BstXI    BstYI  BstZ17I    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EarI     EciI  EcoICRI    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MscI     MslI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PmeI     PmlI     PpiI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PvuI    PvuII     SacI     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     StyI     SwaI    TaqII     TstI     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI