(Circular) (Six-base) MAPSORT of: pFN21AB8351.seq Check: 6151 from: 1 to: 5527 pFN21AB8351 5527 bp With 182 enzymes: SgfI * August 16, 2010 14:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3337 278 Size: 53 83 186 936 1801 2468 Fragments arranged by size: 2468 1801 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2870 1937 Size: 933 4594 Acc65I G'GTAC_C Cuts at: 2848 2848 Size: 5527 AclI AA'CG_TT Cuts at: 1563 3654 4027 1563 Size: 2091 373 3063 Fragments arranged by size: 3063 2091 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3590 4974 5242 1632 Size: 1958 1384 268 1917 Fragments arranged by size: 1958 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5330 AflIII A'CryG_T Cuts at: 1203 2721 4426 5403 1203 Size: 1518 1705 977 1327 Fragments arranged by size: 1705 1518 1327 977 AgeI A'CCGG_T Cuts at: 4358 4358 Size: 5527 AhdI GACnn_n'nnGTC Cuts at: 4256 4256 Size: 5527 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5527 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5527 AlwNI CAG_nnn'CTG Cuts at: 2131 3270 4842 2131 Size: 1139 1572 2816 Fragments arranged by size: 2816 1572 1139 ApaLI G'TGCA_C Cuts at: 3584 4740 3584 Size: 1156 4371 ApoI r'AATT_y Cuts at: 1493 2836 3062 3214 5323 1493 Size: 1343 226 152 2109 1697 Fragments arranged by size: 2109 1697 1343 226 152 AseI AT'TA_AT Cuts at: 160 1032 4081 160 Size: 872 3049 1606 Fragments arranged by size: 3049 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5527 AvaI C'yCGr_G Cuts at: 1870 1943 2354 2852 1870 Size: 73 411 498 4545 Fragments arranged by size: 4545 498 411 73 BamHI G'GATC_C Cuts at: 2857 3224 2857 Size: 367 5160 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2153 2285 2326 2644 2848 Size: 325 217 627 366 132 41 318 204 Cuts at: 2848 4303 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 366 325 318 217 204 132 41 BanII G_rGCy'C Cuts at: 1391 2055 2846 1391 Size: 664 791 4072 Fragments arranged by size: 4072 791 664 BbeI G_GCGC'C Cuts at: 2289 2289 Size: 5527 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4556 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2378 2412 3718 3752 1903 Size: 34 441 34 1306 34 3678 Fragments arranged by size: 3678 1306 441 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3419 4629 3419 Size: 1210 4317 BclI T'GATC_A Cuts at: 1548 2499 1548 Size: 951 4576 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2120 4138 136 Size: 107 122 71 631 605 448 2018 1525 Fragments arranged by size: 2018 1525 631 605 448 122 107 71 BglII A'GATC_T Cuts at: 2358 5522 2358 Size: 3164 2363 BlpI GC'TnA_GC Cuts at: 2944 2944 Size: 5527 Bme1580I G_kGCm'C Cuts at: 1790 2156 2331 2607 2649 3588 4744 1790 Size: 366 175 276 42 939 1156 2573 Fragments arranged by size: 2573 1156 939 366 276 175 42 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5527 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3258 4216 442 Size: 416 53 83 399 24 1841 958 1753 Fragments arranged by size: 1841 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5527 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4187 1239 Size: 2948 2579 Bpu10I CC'TnA_GC Cuts at: 2465 2465 Size: 5527 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3652 4517 4815 3652 Size: 865 298 4364 Fragments arranged by size: 4364 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4190 882 Size: 617 2691 2219 Fragments arranged by size: 2691 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3318 493 Size: 711 2114 2702 Fragments arranged by size: 2702 2114 711 BsaBI GATnn'nnATC Cuts at: 5521 5521 Size: 5527 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2112 2267 2286 3334 Size: 53 83 186 936 579 155 19 1048 Cuts at: 3334 3716 4351 275 Size: 382 635 1451 Fragments arranged by size: 1451 1048 936 635 579 382 186 155 83 53 19 BsaWI w'CCGG_w Cuts at: 3960 4358 4632 4779 5190 3960 Size: 398 274 147 411 4297 Fragments arranged by size: 4297 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2133 2163 2438 2468 1178 Size: 30 165 30 730 30 275 30 4237 Fragments arranged by size: 4237 730 275 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2711 2834 1186 Size: 1525 123 3879 Fragments arranged by size: 3879 1525 123 BseYI C'CCAG_C Cuts at: 2124 2553 2679 2769 4730 5226 2124 Size: 429 126 90 1961 496 2425 Fragments arranged by size: 2425 1961 496 429 126 90 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2305 1163 Size: 573 569 4385 Fragments arranged by size: 4385 573 569 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2392 2667 2977 3738 4766 1540 Size: 275 186 391 275 310 761 1028 2301 Fragments arranged by size: 2301 1028 761 391 310 275 275 186 BsiHKAI G_wGCw'C Cuts at: 2055 3588 3673 4744 2055 Size: 1533 85 1071 2838 Fragments arranged by size: 2838 1533 1071 85 BsmI GAATG_Cn' Cuts at: 3038 3131 3038 Size: 93 5434 BsmBI CGTCTCn'nnnn_ Cuts at: 2258 2258 Size: 5527 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2055 2156 2331 2607 2649 2846 3588 Size: 399 265 101 175 276 42 197 742 Cuts at: 3588 3673 4744 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 276 265 197 175 101 85 42 BspEI T'CCGG_A Cuts at: 5190 5190 Size: 5527 BspHI T'CATG_A Cuts at: 3416 5146 3416 Size: 1730 3797 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2089 2882 844 Size: 1245 793 3489 Fragments arranged by size: 3489 1245 793 BsrBI CCG'CTC Cuts at: 1394 1823 2469 2754 3414 1394 Size: 429 646 285 660 3507 Fragments arranged by size: 3507 660 646 429 285 BsrDI GCAATG_nn' Cuts at: 65 1248 2394 4022 4196 65 Size: 1183 1146 1628 174 1396 Fragments arranged by size: 1628 1396 1183 1146 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2035 4171 4358 1700 Size: 110 225 2136 187 2869 Fragments arranged by size: 2869 2136 225 187 110 BsrGI T'GTAC_A Cuts at: 96 2040 96 Size: 1944 3583 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5527 BssSI C'ACGA_G Cuts at: 3587 4599 3587 Size: 1012 4515 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5527 BstBI TT'CG_AA Cuts at: 4331 4331 Size: 5527 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5527 BstYI r'GATC_y Cuts at: 1972 2358 2857 3224 3609 3626 5078 5164 5176 Size: 386 499 367 385 17 1452 86 12 Cuts at: 5176 5522 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 499 386 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2429 2429 Size: 5527 BtgI C'CryG_G Cuts at: 513 1065 2004 2157 2271 2382 2538 2559 2649 Size: 552 939 153 114 111 156 21 90 Cuts at: 2649 513 Size: 3391 Fragments arranged by size: 3391 939 552 156 153 114 111 90 21 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4788 Fragments arranged by size: 4788 683 56 BtsI GCAGTG_nn' Cuts at: 2154 2317 3043 3837 3857 2154 Size: 163 726 794 20 3824 Fragments arranged by size: 3824 794 726 163 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5527 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5492 DraI TTT'AAA Cuts at: 2831 2831 Size: 5527 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4534 809 Size: 507 3218 1802 Fragments arranged by size: 3218 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2196 2664 2974 Size: 54 1425 50 275 18 366 468 310 Cuts at: 2974 3863 5492 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 468 366 310 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2664 2974 1537 Size: 275 852 310 4090 Fragments arranged by size: 4090 852 310 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3457 1353 Size: 2104 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4114 4488 4634 387 Size: 1064 138 2525 374 146 1280 Fragments arranged by size: 2525 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2053 2053 Size: 5527 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3590 4187 4974 5242 1239 Size: 393 1958 597 787 268 1524 Fragments arranged by size: 1958 1524 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2432 2432 Size: 5527 EcoO109I rG'GnC_Cy Cuts at: 2701 2701 Size: 5527 EcoRI G'AATT_C Cuts at: 2836 3214 2836 Size: 378 5149 FspI TGC'GCA Cuts at: 4033 4033 Size: 5527 HaeII r_GCGC'y Cuts at: 1678 2289 4674 1678 Size: 611 2385 2531 Fragments arranged by size: 2531 2385 611 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2157 2189 4168 4200 4242 Size: 32 135 32 585 32 1979 32 42 Cuts at: 4242 4274 1373 Size: 32 2626 Fragments arranged by size: 2626 1979 585 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2871 3117 669 Size: 988 281 933 246 3079 Fragments arranged by size: 3079 988 933 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5527 HpaI GTT'AAC Cuts at: 3117 3117 Size: 5527 KasI G'GCGC_C Cuts at: 2285 2285 Size: 5527 KpnI G_GTAC'C Cuts at: 2852 2852 Size: 5527 MfeI C'AATT_G Cuts at: 3126 3126 Size: 5527 MluI A'CGCG_T Cuts at: 5403 5403 Size: 5527 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4641 4825 1305 Size: 3336 184 2007 Fragments arranged by size: 3336 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2198 10 Size: 54 1425 343 366 3339 Fragments arranged by size: 3339 1425 366 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2177 2543 2615 3485 3844 4003 518 Size: 648 1011 366 72 870 359 159 2042 Fragments arranged by size: 2042 1011 870 648 366 359 159 72 MspA1I CmG'CkG Cuts at: 1606 2095 2107 2131 2301 2534 2624 2688 2943 Size: 489 12 24 170 233 90 64 255 Cuts at: 2943 3620 4768 5013 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 489 255 245 233 170 90 64 24 12 NarI GG'CG_CC Cuts at: 2286 2286 Size: 5527 NcoI C'CATG_G Cuts at: 513 1065 2004 2382 2538 513 Size: 552 939 378 156 3502 Fragments arranged by size: 3502 939 552 378 156 NdeI CA'TA_TG Cuts at: 387 387 Size: 5527 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5527 NotI GC'GGCC_GC Cuts at: 2974 2974 Size: 5527 NspI r_CATG'y Cuts at: 1132 1229 2725 2885 4430 1132 Size: 97 1496 160 1545 2229 Fragments arranged by size: 2229 1545 1496 160 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5527 PciI A'CATG_T Cuts at: 2721 4426 2721 Size: 1705 3822 PflMI CCAn_nnn'nTGG Cuts at: 1108 2560 1108 Size: 1452 4075 PfoI T'CCnGG_A Cuts at: 2418 2715 2418 Size: 297 5230 PmeI GTTT'AAAC Cuts at: 2831 2831 Size: 5527 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3596 3628 5109 5141 3596 Size: 32 1481 32 3982 Fragments arranged by size: 3982 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2701 2701 Size: 5527 PshAI GACnn'nnGTC Cuts at: 1613 2015 1613 Size: 402 5125 PsiI TTA'TAA Cuts at: 3097 3097 Size: 5527 PstI C_TGCA'G Cuts at: 830 2136 2811 2879 830 Size: 1306 675 68 3478 Fragments arranged by size: 3478 1306 675 68 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5527 PvuII CAG'CTG Cuts at: 2107 2131 2534 2107 Size: 24 403 5100 Fragments arranged by size: 5100 403 24 SacI G_AGCT'C Cuts at: 2055 2055 Size: 5527 SalI G'TCGA_C Cuts at: 1936 2869 1936 Size: 933 4594 SbfI CC_TGCA'GG Cuts at: 2879 2879 Size: 5527 ScaI AGT'ACT Cuts at: 3775 5487 3775 Size: 1712 3815 SfcI C'TryA_G Cuts at: 826 1045 2132 2629 2689 2807 2875 4010 4691 Size: 219 1087 497 60 118 68 1135 681 Cuts at: 4691 4882 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1087 681 497 219 191 118 68 60 SfoI GGC'GCC Cuts at: 2287 2287 Size: 5527 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5527 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5527 SmaI CCC'GGG Cuts at: 1872 2854 1872 Size: 982 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2354 3631 4532 4794 820 Size: 197 926 411 1277 901 262 1553 Fragments arranged by size: 1553 1277 926 901 411 262 197 SnaBI TAC'GTA Cuts at: 493 3318 493 Size: 2825 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5527 SphI G_CATG'C Cuts at: 1132 2885 1132 Size: 1753 3774 SspI AAT'ATT Cuts at: 5 52 3451 5 Size: 47 3399 2081 Fragments arranged by size: 3399 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5527 StyI C'CwwG_G Cuts at: 513 1065 2004 2382 2538 2966 513 Size: 552 939 378 156 428 3074 Fragments arranged by size: 3074 939 552 428 378 156 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3554 3571 3724 3909 4354 325 Size: 1255 1974 17 153 185 445 1498 Fragments arranged by size: 1974 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2040 3773 5485 Size: 274 80 33 51 1443 63 1733 1712 Cuts at: 5485 96 Size: 138 Fragments arranged by size: 1733 1712 1443 274 138 80 63 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2133 2165 2438 2470 5487 5519 2133 Size: 32 273 32 3017 32 2141 Fragments arranged by size: 3017 2141 273 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5527 XbaI T'CTAG_A Cuts at: 2863 2863 Size: 5527 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2591 2591 Size: 5527 XhoI C'TCGA_G Cuts at: 1943 2354 1943 Size: 411 5116 XmaI C'CCGG_G Cuts at: 1870 2852 1870 Size: 982 4545 XmnI GAAnn'nnTTC Cuts at: 3656 3656 Size: 5527 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3335 276 Size: 53 83 186 936 1801 2468 Fragments arranged by size: 2468 1801 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BstXI BstZ17I DraIII EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI