Restriction map for
pFN21AB8351
(Linear) (Six-base) MAPSORT of: KIBB8351.seq Check: 8508 from: 1 to: 822
KIBB8351.orf
With 182 enzymes: SgfI *
March 2, 2010 14:28 ..
AflIII A'CryG_T
Cuts at: 0 716 822
Size: 716 106
AlwNI CAG_nnn'CTG
Cuts at: 0 126 822
Size: 126 696
AvaI C'yCGr_G
Cuts at: 0 349 822
Size: 349 473
BanI G'GyrC_C
Cuts at: 0 148 280 321 639 822
Size: 148 132 41 318 183
Fragments arranged by size:
318 183 148 132 41
BanII G_rGCy'C
Cuts at: 0 50 822
Size: 50 772
BbeI G_GCGC'C
Cuts at: 0 284 822
Size: 284 538
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 373 407 822
Size: 373 34 415
Fragments arranged by size:
415 373 34
BclI T'GATC_A
Cuts at: 0 494 822
Size: 494 328
BglI GCCn_nnn'nGGC
Cuts at: 0 115 822
Size: 115 707
BglII A'GATC_T
Cuts at: 0 353 822
Size: 353 469
Bme1580I G_kGCm'C
Cuts at: 0 151 326 602 644 822
Size: 151 175 276 42 178
Fragments arranged by size:
276 178 175 151 42
Bpu10I CC'TnA_GC
Cuts at: 0 460 822
Size: 460 362
BsaHI Gr'CG_yC
Cuts at: 0 107 262 281 822
Size: 107 155 19 541
Fragments arranged by size:
541 155 107 19
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 128 158 433 463 822
Size: 128 30 275 30 359
Fragments arranged by size:
359 275 128 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 706 822
Size: 706 116
BseYI C'CCAG_C
Cuts at: 0 119 548 674 764 822
Size: 119 429 126 90 58
Fragments arranged by size:
429 126 119 90 58
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 300 822
Size: 300 522
BsiEI CG_ry'CG
Cuts at: 0 387 662 822
Size: 387 275 160
Fragments arranged by size:
387 275 160
BsiHKAI G_wGCw'C
Cuts at: 0 50 822
Size: 50 772
BsmBI CGTCTCn'nnnn_
Cuts at: 0 253 822
Size: 253 569
Bsp1286I G_dGCh'C
Cuts at: 0 50 151 326 602 644 822
Size: 50 101 175 276 42 178
Fragments arranged by size:
276 178 175 101 50 42
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 84 822
Size: 84 738
BsrBI CCG'CTC
Cuts at: 0 464 749 822
Size: 464 285 73
Fragments arranged by size:
464 285 73
BsrDI GCAATG_nn'
Cuts at: 0 389 822
Size: 389 433
BsrFI r'CCGG_y
Cuts at: 0 30 822
Size: 30 792
BsrGI T'GTAC_A
Cuts at: 0 35 822
Size: 35 787
BstYI r'GATC_y
Cuts at: 0 353 822
Size: 353 469
Bsu36I CC'TnA_GG
Cuts at: 0 424 822
Size: 424 398
BtgI C'CryG_G
Cuts at: 0 152 266 377 533 554 644 822
Size: 152 114 111 156 21 90 178
Fragments arranged by size:
178 156 152 114 111 90 21
BtsI GCAGTG_nn'
Cuts at: 0 149 312 822
Size: 149 163 510
Fragments arranged by size:
510 163 149
EaeI y'GGCC_r
Cuts at: 0 191 659 822
Size: 191 468 163
Fragments arranged by size:
468 191 163
EagI C'GGCC_G
Cuts at: 0 659 822
Size: 659 163
EcoICRI GAG'CTC
Cuts at: 0 48 822
Size: 48 774
EcoNI CCTnn'n_nnAGG
Cuts at: 0 427 822
Size: 427 395
EcoO109I rG'GnC_Cy
Cuts at: 0 696 822
Size: 696 126
HaeII r_GCGC'y
Cuts at: 0 284 822
Size: 284 538
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 152 184 822
Size: 152 32 638
Fragments arranged by size:
638 152 32
KasI G'GCGC_C
Cuts at: 0 280 822
Size: 280 542
MscI TGG'CCA
Cuts at: 0 193 822
Size: 193 629
MslI CAynn'nnrTG
Cuts at: 0 172 538 610 822
Size: 172 366 72 212
Fragments arranged by size:
366 212 172 72
MspA1I CmG'CkG
Cuts at: 0 90 102 126 296 529 619 683 822
Size: 90 12 24 170 233 90 64 139
Fragments arranged by size:
233 170 139 90 90 64 24 12
NarI GG'CG_CC
Cuts at: 0 281 822
Size: 281 541
NcoI C'CATG_G
Cuts at: 0 377 533 822
Size: 377 156 289
Fragments arranged by size:
377 289 156
NspI r_CATG'y
Cuts at: 0 720 822
Size: 720 102
PciI A'CATG_T
Cuts at: 0 716 822
Size: 716 106
PflMI CCAn_nnn'nTGG
Cuts at: 0 555 822
Size: 555 267
PfoI T'CCnGG_A
Cuts at: 0 413 710 822
Size: 413 297 112
Fragments arranged by size:
413 297 112
PpuMI rG'GwC_Cy
Cuts at: 0 696 822
Size: 696 126
PshAI GACnn'nnGTC
Cuts at: 0 10 822
Size: 10 812
PstI C_TGCA'G
Cuts at: 0 131 806 822
Size: 131 675 16
Fragments arranged by size:
675 131 16
PvuII CAG'CTG
Cuts at: 0 102 126 529 822
Size: 102 24 403 293
Fragments arranged by size:
403 293 102 24
SacI G_AGCT'C
Cuts at: 0 50 822
Size: 50 772
SfcI C'TryA_G
Cuts at: 0 127 624 684 802 822
Size: 127 497 60 118 20
Fragments arranged by size:
497 127 118 60 20
SfoI GGC'GCC
Cuts at: 0 282 822
Size: 282 540
SmlI C'TyrA_G
Cuts at: 0 349 822
Size: 349 473
StyI C'CwwG_G
Cuts at: 0 377 533 822
Size: 377 156 289
Fragments arranged by size:
377 289 156
TatI w'GTAC_w
Cuts at: 0 35 822
Size: 35 787
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 128 160 433 465 822
Size: 128 32 273 32 357
Fragments arranged by size:
357 273 128 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 586 822
Size: 586 236
XhoI C'TCGA_G
Cuts at: 0 349 822
Size: 349 473
Enzymes that do cut:
AflIII AlwNI AvaI BanI BanII BbeI BcgI BclI
BglI BglII Bme1580I Bpu10I BsaHI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrDI
BsrFI BsrGI BstYI Bsu36I BtgI BtsI EaeI EagI
EcoICRI EcoNI EcoO109I HaeII Hin4I KasI MscI MslI
MspA1I NarI NcoI NspI PciI PflMI PfoI PpuMI
PshAI PstI PvuII SacI SfcI SfoI SmlI StyI
TatI TstI XcmI XhoI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AgeI AhdI AleI AlfI AloI ApaI ApaLI ApoI
AscI AseI AsiSI AvrII BaeI BamHI BbsI BbvCI
BciVI BfrBI BlpI BmgBI BmrI BmtI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaWI BsiWI BsmI BspEI
BspHI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I
BtgZI ClaI CspCI DraI DraIII DrdI EarI EciI
Eco57MI EcoRI EcoRV FalI FseI FspI FspAI HincII
HindIII HpaI KpnI MfeI MluI MmeI NaeI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PmeI
PmlI PpiI PsiI PspOMI PspXI PsrI PvuI RsrII
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
StuI SwaI TaqII Tth111I XbaI XmaI XmnI ZraI