Restriction map for
pFN21AE0165
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# NO CUT ENZYMES ( 155 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AfaI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BamHI BanI BbeI BbrPI
BbvCI BcgI BciVI BclI BfaI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaI BsaWI
BseAI BsiEI BsiWI BsmBI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrDI BsrFI BsrGI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraI DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI KasI KpnI KspI
MaeI MamI MfeI MluI MroI
MslI MspA1I MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsaI RsrII SacI
SacII SalI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI SspBI SspI StuI
SwaI TfiI Tsp509I Tth111I Van91I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccII 109 CGCG CG^CG
AcyI 361 GACGCC G[GA]^CG[CT]C
AlwI 408 GGATC GGATC(4/5)
AlwNI 187 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 374 GGGCCC GGGCC^C
AspHI 97 GAGCAC G[AT]GC[AT]^C
BalI 68 TGGCCA TGG^CCA
BbsI 442 GAAGAC GAAGAC(2/6)
BpmI 480 CTGGAG CTGGAG(16/14)
BpuAI 442 GAAGAC GAAGAC(2/6)
BsaHI 361 GACGCC G[GA]^CG[CT]C
BsgI 205 CTGCAC GTGCAG(16/14)
BsiHKAI 97 GAGCAC G[AT]GC[AT]^C
BsmFI 171 GTCCC GGGAC(10/14)
BsmI 82 GAATGC GAATGC(1/-1)
BsrBI 457 GAGCGG CCGCTC(-3/-3)
BsrI 479 ACTGG ACTGG(1/-1)
BssSI 100 CACGAG CACGAG(-5/-1)
BstUI 109 CGCG CG^CG
BstYI 408 GGATCT [GA]^GATC[CT]
DraIII 394 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 68 TGGCCA [CT]^GGCC[GA]
EarI 54 GAAGAG CTCTTC(1/4)
HaeII 326 AGCGCC [GA]GCGC^[CT]
Hin1I 361 GACGCC G[GA]^CG[CT]C
HinfI 344 GAGTC G^A[ACGT]TC
Ksp632I 54 GAAGAG CTCTTC(1/4)
MaeII 491 ACGT A^CGT
MaeIII 476 GTGAC ^GT[ACGT]AC
MflI 408 GGATCT [GA]^GATC[CT]
MluNI 68 TGGCCA TGG^CCA
MscI 68 TGGCCA TGG^CCA
MseI 281 TTAA T^TAA
MvnI 109 CGCG CG^CG
NcoI 404 CCATGG C^CATGG
PleI 344 GAGTC GAGTC(4/5)
SapI 54 GAAGAGC GCTCTTC(1/4)
SbfI 183 CCTGCAGG CCTGCA^GG
SmlI 424 CTCAAG C^T[CT][GA]AG
Sse8387I 183 CCTGCAGG CCTGCA^GG
Tru9I 281 TTAA T^TAA
Tsp45I 476 GTGAC ^GT[GC]AC
XhoII 408 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AluI 203 AGCT AG^CT
293 AGCT
AvaII 189 GGACC G^G[AT]CC
285 GGACC
BanII 374 GGGCCC G[GA]GC[CT]^C
384 GGGCTC
BbvI 153 GCTGC GCAGC(8/12)
204 GCTGC
BcnI 198 CCGGG CC^[GC]GG
372 CCGGG
BseRI 103 GAGGAG GAGGAG(10/8)
232 GAGGAG
BsmAI 352 GTCTC GTCTC(1/5)
546 GTCTC
BstF5I 144 CATCC GGATG(2/0)
228 GGATG
BstNI 132 CCTGG CC^[AT]GG
272 CCAGG
DdeI 116 CTGAG C^T[ACGT]AG
175 CTCAG
DpnII 27 GATC ^GATC
409 GATC
EcoRII 132 CCTGG ^CC[AT]GG
272 CCAGG
FokI 144 CATCC GGATG(9/13)
228 GGATG
HgaI 361 GACGC GACGC(5/10)
339 GCGTC
HphI 300 GGTGA GGTGA(8/7)
475 GGTGA
MboI 27 GATC ^GATC
409 GATC
MvaI 132 CCTGG CC^[AT]GG
272 CCAGG
MwoI 99 GCACGAGGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
509 GCATGGAAGGC
NciI 198 CCGGG CC^[GC]GG
372 CCGGG
NdeII 27 GATC ^GATC
409 GATC
NlaIV 374 GGGCCC GG[ACGT]^[ACGT]CC
385 GGCTCC
PpuMI 188 AGGACCT [GA]G^G[AT]CC[CT]
284 AGGACCT
PstI 154 CTGCAG CTGCA^G
184 CTGCAG
Sau3AI 27 GATC ^GATC
409 GATC
SfcI 154 CTGCAG C^T[GA][CT]AG
184 CTGCAG
TaqI 11 TCGA T^CGA
342 TCGA
TseI 153 GCTGC G^C[AT]GC
204 GCTGC
TspRI 30 CACTG CA[GC]TG[ACGT][ACGT]^
177 CAGTG
TthHB8I 11 TCGA T^CGA
342 TCGA
XcmI 212 CCAAGGTGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
500 CCATGTCTGGCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 30 enzymes) #
#########################################
AciI BmyI BsaJI BsiYI BslI
Bsp1286I BssKI Cac8I CfoI Cfr13I
DraII DsaI EcoO109I EcoT14I FauI
Fnu4HI HaeIII HapII HhaI HinP1I
HpaII ItaI MboII MnlI MspI
NlaIII Sau96I ScrFI SfaNI StyI