(Circular) (Six-base) MAPSORT of: pFN21AE0165.seq Check: 8889 from: 1 to: 5266 pFN21AE0165 5266 bp With 182 enzymes: SgfI * October 20, 2009 10:45 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3076 278 Size: 53 83 186 936 1540 2468 Fragments arranged by size: 2468 1540 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2609 1937 Size: 672 4594 Acc65I G'GTAC_C Cuts at: 2587 2587 Size: 5266 AclI AA'CG_TT Cuts at: 1563 3393 3766 1563 Size: 1830 373 3063 Fragments arranged by size: 3063 1830 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2078 2315 2321 3329 4713 4981 1632 Size: 446 237 6 1008 1384 268 1917 Fragments arranged by size: 1917 1384 1008 446 268 237 6 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5069 AflIII A'CryG_T Cuts at: 1203 4165 5142 1203 Size: 2962 977 1327 Fragments arranged by size: 2962 1327 977 AgeI A'CCGG_T Cuts at: 4097 4097 Size: 5266 AhdI GACnn_n'nnGTC Cuts at: 3995 3995 Size: 5266 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5266 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2069 1184 Size: 885 4381 AlwNI CAG_nnn'CTG Cuts at: 2197 3009 4581 2197 Size: 812 1572 2882 Fragments arranged by size: 2882 1572 812 ApaI G_GGCC'C Cuts at: 2383 2383 Size: 5266 ApaLI G'TGCA_C Cuts at: 3323 4479 3323 Size: 1156 4110 ApoI r'AATT_y Cuts at: 1493 2575 2801 2953 5062 1493 Size: 1082 226 152 2109 1697 Fragments arranged by size: 2109 1697 1082 226 152 AseI AT'TA_AT Cuts at: 160 1032 3820 160 Size: 872 2788 1606 Fragments arranged by size: 2788 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5266 AvaI C'yCGr_G Cuts at: 1870 1943 2591 1870 Size: 73 648 4545 Fragments arranged by size: 4545 648 73 BamHI G'GATC_C Cuts at: 2596 2963 2596 Size: 367 4899 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2587 4042 618 Size: 325 217 627 800 1455 1842 Fragments arranged by size: 1842 1455 800 627 325 217 BanII G_rGCy'C Cuts at: 1391 2383 2393 2585 1391 Size: 992 10 192 4072 Fragments arranged by size: 4072 992 192 10 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2454 928 Size: 971 555 3740 Fragments arranged by size: 3740 971 555 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3457 3491 1903 Size: 34 50 34 1436 34 3678 Fragments arranged by size: 3678 1436 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3158 4368 3158 Size: 1210 4056 BclI T'GATC_A Cuts at: 1548 1548 Size: 5266 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3877 136 Size: 107 122 71 631 605 2205 1525 Fragments arranged by size: 2205 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5261 5261 Size: 5266 BlpI GC'TnA_GC Cuts at: 2683 2683 Size: 5266 Bme1580I G_kGCm'C Cuts at: 1790 2188 2383 3327 4483 1790 Size: 398 195 944 1156 2573 Fragments arranged by size: 2573 1156 944 398 195 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5266 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2997 3955 442 Size: 416 53 83 399 24 1580 958 1753 Fragments arranged by size: 1753 1580 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5266 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2506 3926 1239 Size: 1267 1420 2579 Fragments arranged by size: 2579 1420 1267 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2414 3391 4256 4554 2414 Size: 977 865 298 3126 Fragments arranged by size: 3126 977 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3929 882 Size: 617 2430 2219 Fragments arranged by size: 2430 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3057 493 Size: 711 1853 2702 Fragments arranged by size: 2702 1853 711 BsaBI GATnn'nnATC Cuts at: 5260 5260 Size: 5266 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2367 3073 3455 4090 Size: 53 83 186 936 834 706 382 635 Cuts at: 4090 275 Size: 1451 Fragments arranged by size: 1451 936 834 706 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3699 4097 4371 4518 4929 3699 Size: 398 274 147 411 4036 Fragments arranged by size: 4036 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2492 2522 1178 Size: 30 165 30 1089 30 3922 Fragments arranged by size: 3922 1089 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2123 2252 1186 Size: 937 129 4200 Fragments arranged by size: 4200 937 129 BseYI C'CCAG_C Cuts at: 2091 2386 4469 4965 2091 Size: 295 2083 496 2392 Fragments arranged by size: 2392 2083 496 295 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2195 1163 Size: 573 459 4234 Fragments arranged by size: 4234 573 459 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2716 3477 4505 1540 Size: 275 186 715 761 1028 2301 Fragments arranged by size: 2301 1028 761 715 275 186 BsiHKAI G_wGCw'C Cuts at: 2106 3327 3412 4483 2106 Size: 1221 85 1071 2889 Fragments arranged by size: 2889 1221 1071 85 BsmI GAATG_Cn' Cuts at: 2093 2777 2870 2093 Size: 684 93 4489 Fragments arranged by size: 4489 684 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2106 2188 2383 2393 2585 3327 3412 Size: 399 316 82 195 10 192 742 85 Cuts at: 3412 4483 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 316 195 192 85 82 10 BspEI T'CCGG_A Cuts at: 4929 4929 Size: 5266 BspHI T'CATG_A Cuts at: 3155 4885 3155 Size: 1730 3536 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2621 844 Size: 1777 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2464 3153 1394 Size: 429 641 689 3507 Fragments arranged by size: 3507 689 641 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3761 3935 65 Size: 1183 2513 174 1396 Fragments arranged by size: 2513 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3910 4097 1700 Size: 110 2100 187 2869 Fragments arranged by size: 2869 2100 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5266 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5266 BssSI C'ACGA_G Cuts at: 2105 3326 4338 2105 Size: 1221 1012 3033 Fragments arranged by size: 3033 1221 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5266 BstBI TT'CG_AA Cuts at: 4070 4070 Size: 5266 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5266 BstYI r'GATC_y Cuts at: 1972 2413 2596 2963 3348 3365 4817 4903 4915 Size: 441 183 367 385 17 1452 86 12 Cuts at: 4915 5261 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 441 385 367 346 183 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2167 2317 2409 513 Size: 552 1102 150 92 3370 Fragments arranged by size: 3370 1102 552 150 92 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4527 Fragments arranged by size: 4527 683 56 BtsI GCAGTG_nn' Cuts at: 2034 2782 3576 3596 2034 Size: 748 794 20 3704 Fragments arranged by size: 3704 794 748 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5266 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5231 DraI TTT'AAA Cuts at: 2570 2570 Size: 5266 DraIII CAC_nnn'GTG Cuts at: 2404 2404 Size: 5266 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4273 809 Size: 507 2957 1802 Fragments arranged by size: 2957 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2073 2713 3602 Size: 54 1425 50 275 18 243 640 889 Cuts at: 3602 5231 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 640 275 243 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2713 1537 Size: 275 901 4090 Fragments arranged by size: 4090 901 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2054 3196 1353 Size: 701 1142 3423 Fragments arranged by size: 3423 1142 701 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3853 4227 4373 387 Size: 1064 138 2264 374 146 1280 Fragments arranged by size: 2264 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2078 2315 2321 2506 3329 3926 4713 Size: 393 446 237 6 185 823 597 787 Cuts at: 4713 4981 1239 Size: 268 1524 Fragments arranged by size: 1524 823 787 597 446 393 268 237 185 6 EcoO109I rG'GnC_Cy Cuts at: 2194 2290 2380 2194 Size: 96 90 5080 Fragments arranged by size: 5080 96 90 EcoRI G'AATT_C Cuts at: 2575 2953 2575 Size: 378 4888 FspI TGC'GCA Cuts at: 3772 3772 Size: 5266 HaeII r_GCGC'y Cuts at: 1678 2335 4413 1678 Size: 657 2078 2531 Fragments arranged by size: 2531 2078 657 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2340 2372 2492 2524 3907 Size: 32 135 32 768 32 120 32 1383 Cuts at: 3907 3939 3981 4013 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1383 768 135 120 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2610 2856 669 Size: 988 281 672 246 3079 Fragments arranged by size: 3079 988 672 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5266 HpaI GTT'AAC Cuts at: 2856 2856 Size: 5266 KpnI G_GTAC'C Cuts at: 2591 2591 Size: 5266 MfeI C'AATT_G Cuts at: 2865 2865 Size: 5266 MluI A'CGCG_T Cuts at: 5142 5142 Size: 5266 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2579 4380 4564 1305 Size: 1274 1801 184 2007 Fragments arranged by size: 2007 1801 1274 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2075 10 Size: 54 1425 343 243 3201 Fragments arranged by size: 3201 1425 343 243 54 MslI CAynn'nnrTG Cuts at: 518 1166 3224 3583 3742 518 Size: 648 2058 359 159 2042 Fragments arranged by size: 2058 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2682 3359 4507 4752 1606 Size: 1076 677 1148 245 2120 Fragments arranged by size: 2120 1148 1076 677 245 NcoI C'CATG_G Cuts at: 513 1065 2409 513 Size: 552 1344 3370 Fragments arranged by size: 3370 1344 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5266 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5266 NotI GC'GGCC_GC Cuts at: 2713 2713 Size: 5266 NspI r_CATG'y Cuts at: 1132 1229 2624 4169 1132 Size: 97 1395 1545 2229 Fragments arranged by size: 2229 1545 1395 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5266 PciI A'CATG_T Cuts at: 4165 4165 Size: 5266 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5266 PmeI GTTT'AAAC Cuts at: 2570 2570 Size: 5266 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3335 3367 4848 4880 3335 Size: 32 1481 32 3721 Fragments arranged by size: 3721 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2194 2290 2194 Size: 96 5170 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5266 PsiI TTA'TAA Cuts at: 2836 2836 Size: 5266 PspOMI G'GGCC_C Cuts at: 2379 2379 Size: 5266 PstI C_TGCA'G Cuts at: 830 2163 2193 2618 830 Size: 1333 30 425 3478 Fragments arranged by size: 3478 1333 425 30 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5266 SalI G'TCGA_C Cuts at: 1936 2608 1936 Size: 672 4594 SapI GCTCTTCn'nnn_ Cuts at: 2054 2054 Size: 5266 SbfI CC_TGCA'GG Cuts at: 2193 2618 2193 Size: 425 4841 ScaI AGT'ACT Cuts at: 3514 5226 3514 Size: 1712 3554 SfcI C'TryA_G Cuts at: 826 1045 2159 2189 2614 3749 4430 4621 826 Size: 219 1114 30 425 1135 681 191 1471 Fragments arranged by size: 1471 1135 1114 681 425 219 191 30 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5266 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5266 SmaI CCC'GGG Cuts at: 1872 2593 1872 Size: 721 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2429 3370 4271 4533 820 Size: 197 926 486 941 901 262 1553 Fragments arranged by size: 1553 941 926 901 486 262 197 SnaBI TAC'GTA Cuts at: 493 3057 493 Size: 2564 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5266 SphI G_CATG'C Cuts at: 1132 2624 1132 Size: 1492 3774 SspI AAT'ATT Cuts at: 5 52 3190 5 Size: 47 3138 2081 Fragments arranged by size: 3138 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5266 StyI C'CwwG_G Cuts at: 513 1065 2076 2082 2217 2409 2705 513 Size: 552 1011 6 135 192 296 3074 Fragments arranged by size: 3074 1011 552 296 192 135 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2468 3293 3310 3463 3648 4093 325 Size: 1255 888 825 17 153 185 445 1498 Fragments arranged by size: 1498 1255 888 825 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3512 5224 96 Size: 274 80 33 51 1443 1535 1712 138 Fragments arranged by size: 1712 1535 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2390 2422 5226 5258 2390 Size: 32 2804 32 2398 Fragments arranged by size: 2804 2398 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5266 XbaI T'CTAG_A Cuts at: 2602 2602 Size: 5266 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2224 2512 2224 Size: 288 4978 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5266 XmaI C'CCGG_G Cuts at: 1870 2591 1870 Size: 721 4545 XmnI GAAnn'nnTTC Cuts at: 3395 3395 Size: 5266 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3074 276 Size: 53 83 186 936 1540 2468 Fragments arranged by size: 2468 1540 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI