Restriction map for
pFN21AE0165
(Linear) (Six-base) MAPSORT of: KIEE0165.seq Check: 8940 from: 1 to: 561
KIEE0165.orf
With 182 enzymes: SgfI *
April 24, 2009 13:11 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 73 310 316 561
Size: 73 237 6 245
Fragments arranged by size:
245 237 73 6
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 64 561
Size: 64 497
AlwNI CAG_nnn'CTG
Cuts at: 0 192 561
Size: 192 369
ApaI G_GGCC'C
Cuts at: 0 378 561
Size: 378 183
BanII G_rGCy'C
Cuts at: 0 378 388 561
Size: 378 10 173
Fragments arranged by size:
378 173 10
BbsI GAAGACnn'nnnn_
Cuts at: 0 449 561
Size: 449 112
Bme1580I G_kGCm'C
Cuts at: 0 183 378 561
Size: 183 195 183
Fragments arranged by size:
195 183 183
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 501 561
Size: 501 60
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 409 561
Size: 409 152
BsaHI Gr'CG_yC
Cuts at: 0 362 561
Size: 362 199
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 487 517 561
Size: 487 30 44
Fragments arranged by size:
487 44 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 118 247 561
Size: 118 129 314
Fragments arranged by size:
314 129 118
BseYI C'CCAG_C
Cuts at: 0 86 381 561
Size: 86 295 180
Fragments arranged by size:
295 180 86
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 190 561
Size: 190 371
BsiHKAI G_wGCw'C
Cuts at: 0 101 561
Size: 101 460
BsmI GAATG_Cn'
Cuts at: 0 88 561
Size: 88 473
Bsp1286I G_dGCh'C
Cuts at: 0 101 183 378 388 561
Size: 101 82 195 10 173
Fragments arranged by size:
195 173 101 82 10
BsrBI CCG'CTC
Cuts at: 0 459 561
Size: 459 102
BssSI C'ACGA_G
Cuts at: 0 100 561
Size: 100 461
BstYI r'GATC_y
Cuts at: 0 408 561
Size: 408 153
BtgI C'CryG_G
Cuts at: 0 162 312 404 561
Size: 162 150 92 157
Fragments arranged by size:
162 157 150 92
BtsI GCAGTG_nn'
Cuts at: 0 29 561
Size: 29 532
DraIII CAC_nnn'GTG
Cuts at: 0 399 561
Size: 399 162
EaeI y'GGCC_r
Cuts at: 0 68 561
Size: 68 493
EarI CTCTTCn'nnn_
Cuts at: 0 49 561
Size: 49 512
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 73 310 316 501 561
Size: 73 237 6 185 60
Fragments arranged by size:
237 185 73 60 6
EcoO109I rG'GnC_Cy
Cuts at: 0 189 285 375 561
Size: 189 96 90 186
Fragments arranged by size:
189 186 96 90
HaeII r_GCGC'y
Cuts at: 0 330 561
Size: 330 231
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 335 367 487 519 561
Size: 335 32 120 32 42
Fragments arranged by size:
335 120 42 32 32
MscI TGG'CCA
Cuts at: 0 70 561
Size: 70 491
NcoI C'CATG_G
Cuts at: 0 404 561
Size: 404 157
PpuMI rG'GwC_Cy
Cuts at: 0 189 285 561
Size: 189 96 276
Fragments arranged by size:
276 189 96
PspOMI G'GGCC_C
Cuts at: 0 374 561
Size: 374 187
PstI C_TGCA'G
Cuts at: 0 158 188 561
Size: 158 30 373
Fragments arranged by size:
373 158 30
SapI GCTCTTCn'nnn_
Cuts at: 0 49 561
Size: 49 512
SbfI CC_TGCA'GG
Cuts at: 0 188 561
Size: 188 373
SfcI C'TryA_G
Cuts at: 0 154 184 561
Size: 154 30 377
Fragments arranged by size:
377 154 30
SmlI C'TyrA_G
Cuts at: 0 424 561
Size: 424 137
StyI C'CwwG_G
Cuts at: 0 71 77 212 404 561
Size: 71 6 135 192 157
Fragments arranged by size:
192 157 135 71 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 463 561
Size: 463 98
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 385 417 561
Size: 385 32 144
Fragments arranged by size:
385 144 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 219 507 561
Size: 219 288 54
Fragments arranged by size:
288 219 54
Enzymes that do cut:
AcuI AlfI AlwNI ApaI BanII BbsI Bme1580I BpmI
BpuEI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmI
Bsp1286I BsrBI BssSI BstYI BtgI BtsI DraIII EaeI
EarI Eco57MI EcoO109I HaeII Hin4I MscI NcoI PpuMI
PspOMI PstI SapI SbfI SfcI SmlI StyI TaqII
TstI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AloI ApaLI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BbeI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI BmgBI
BmrI BmtI BplI Bpu10I BsaI BsaAI BsaBI BsaWI
BsiEI BsiWI BsmBI BspEI BspHI BspMI BsrDI BsrFI
BsrGI BssHII BstAPI BstBI BstEII BstXI BstZ17I Bsu36I
BtgZI ClaI CspCI DraI DrdI EagI EciI EcoICRI
EcoNI EcoRI EcoRV FalI FseI FspI FspAI HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MslI
MspA1I NaeI NarI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PasI PciI PflMI PfoI PmeI
PmlI PpiI PshAI PsiI PspXI PsrI PvuI PvuII
RsrII SacI SacII SalI SanDI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StuI SwaI TatI Tth111I XbaI XhoI XmaI
XmnI ZraI