(Circular) (Six-base) MAPSORT of: pFN21AE0175.seq Check: 6818 from: 1 to: 5608 pFN21AE0175 5608 bp With 182 enzymes: SgfI * October 20, 2009 10:45 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2054 2378 2447 2848 2054 Size: 324 69 401 4814 Fragments arranged by size: 4814 401 324 69 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3418 278 Size: 53 83 186 936 1882 2468 Fragments arranged by size: 2468 1882 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2951 1937 Size: 1014 4594 Acc65I G'GTAC_C Cuts at: 2929 2929 Size: 5608 AclI AA'CG_TT Cuts at: 1563 3735 4108 1563 Size: 2172 373 3063 Fragments arranged by size: 3063 2172 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2295 2561 2860 3671 5055 5323 1632 Size: 663 266 299 811 1384 268 1917 Fragments arranged by size: 1917 1384 811 663 299 268 266 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5411 AflIII A'CryG_T Cuts at: 1203 4507 5484 1203 Size: 3304 977 1327 Fragments arranged by size: 3304 1327 977 AgeI A'CCGG_T Cuts at: 4439 4439 Size: 5608 AhdI GACnn_n'nnGTC Cuts at: 2744 4337 2744 Size: 1593 4015 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5608 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5608 AlwNI CAG_nnn'CTG Cuts at: 2143 2840 3351 4923 2143 Size: 697 511 1572 2828 Fragments arranged by size: 2828 1572 697 511 ApaLI G'TGCA_C Cuts at: 3665 4821 3665 Size: 1156 4452 ApoI r'AATT_y Cuts at: 1493 2480 2917 3143 3295 5404 1493 Size: 987 437 226 152 2109 1697 Fragments arranged by size: 2109 1697 987 437 226 152 AseI AT'TA_AT Cuts at: 160 1032 4162 160 Size: 872 3130 1606 Fragments arranged by size: 3130 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5608 AvaI C'yCGr_G Cuts at: 1870 1943 2225 2933 1870 Size: 73 282 708 4545 Fragments arranged by size: 4545 708 282 73 BamHI G'GATC_C Cuts at: 2938 3305 2938 Size: 367 5241 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2534 2767 2929 4384 618 Size: 325 217 627 747 233 162 1455 1842 Fragments arranged by size: 1842 1455 747 627 325 233 217 162 BanII G_rGCy'C Cuts at: 1391 2013 2340 2927 1391 Size: 622 327 587 4072 Fragments arranged by size: 4072 622 587 327 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2863 928 Size: 971 964 3673 Fragments arranged by size: 3673 971 964 BbvCI CC'TCA_GC Cuts at: 2701 2701 Size: 5608 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3799 3833 1903 Size: 34 1862 34 3678 Fragments arranged by size: 3678 1862 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2518 3500 4710 2518 Size: 982 1210 3416 Fragments arranged by size: 3416 1210 982 BclI T'GATC_A Cuts at: 1548 1548 Size: 5608 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2711 2720 4219 Size: 107 122 71 631 605 1039 9 1499 Cuts at: 4219 136 Size: 1525 Fragments arranged by size: 1525 1499 1039 631 605 122 107 71 9 BglII A'GATC_T Cuts at: 2423 5603 2423 Size: 3180 2428 BlpI GC'TnA_GC Cuts at: 3025 3025 Size: 5608 Bme1580I G_kGCm'C Cuts at: 1790 2539 3669 4825 1790 Size: 749 1130 1156 2573 Fragments arranged by size: 2573 1156 1130 749 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5608 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2316 3339 4297 Size: 416 53 83 399 24 899 1023 958 Cuts at: 4297 442 Size: 1753 Fragments arranged by size: 1753 1023 958 899 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5608 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2337 4268 1239 Size: 1098 1931 2579 Fragments arranged by size: 2579 1931 1098 Bpu10I CC'TnA_GC Cuts at: 2701 2701 Size: 5608 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2366 3733 4598 4896 2366 Size: 1367 865 298 3078 Fragments arranged by size: 3078 1367 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4271 882 Size: 617 2772 2219 Fragments arranged by size: 2772 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2116 3399 493 Size: 711 912 1283 2702 Fragments arranged by size: 2702 1283 912 711 BsaBI GATnn'nnATC Cuts at: 5602 5602 Size: 5608 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3415 3797 4432 275 Size: 53 83 186 936 1882 382 635 1451 Fragments arranged by size: 1882 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4041 4439 4713 4860 5271 4041 Size: 398 274 147 411 4378 Fragments arranged by size: 4378 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2853 2883 1178 Size: 30 165 30 1450 30 3903 Fragments arranged by size: 3903 1450 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2513 1186 Size: 1327 4281 BseYI C'CCAG_C Cuts at: 2321 2339 2450 2611 4811 5307 2321 Size: 18 111 161 2200 496 2622 Fragments arranged by size: 2622 2200 496 161 111 18 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2412 1163 Size: 573 676 4359 Fragments arranged by size: 4359 676 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3058 3819 4847 1540 Size: 275 186 1057 761 1028 2301 Fragments arranged by size: 2301 1057 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2013 3669 3754 4825 2013 Size: 1656 85 1071 2796 Fragments arranged by size: 2796 1656 1071 85 BsmI GAATG_Cn' Cuts at: 3119 3212 3119 Size: 93 5515 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2013 2340 2539 2927 3669 3754 4825 Size: 399 223 327 199 388 742 85 1071 Cuts at: 4825 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 388 327 223 199 85 BspEI T'CCGG_A Cuts at: 5271 5271 Size: 5608 BspHI T'CATG_A Cuts at: 3497 5227 3497 Size: 1730 3878 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2054 2378 2447 2848 2963 844 Size: 1210 324 69 401 115 3489 Fragments arranged by size: 3489 1210 401 324 115 69 BsrBI CCG'CTC Cuts at: 1394 1823 2670 3495 1394 Size: 429 847 825 3507 Fragments arranged by size: 3507 847 825 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4103 4277 65 Size: 1183 2855 174 1396 Fragments arranged by size: 2855 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4252 4439 1700 Size: 110 2442 187 2869 Fragments arranged by size: 2869 2442 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5608 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5608 BssSI C'ACGA_G Cuts at: 3668 4680 3668 Size: 1012 4596 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5608 BstBI TT'CG_AA Cuts at: 4412 4412 Size: 5608 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5608 BstYI r'GATC_y Cuts at: 1972 2423 2938 3305 3690 3707 5159 5245 5257 Size: 451 515 367 385 17 1452 86 12 Cuts at: 5257 5603 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 515 451 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2529 513 Size: 552 939 525 3592 Fragments arranged by size: 3592 939 552 525 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4869 Fragments arranged by size: 4869 683 56 BtsI GCAGTG_nn' Cuts at: 2621 2659 3124 3918 3938 2621 Size: 38 465 794 20 4291 Fragments arranged by size: 4291 794 465 38 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5608 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5573 DraI TTT'AAA Cuts at: 2912 2912 Size: 5608 DraIII CAC_nnn'GTG Cuts at: 2206 2206 Size: 5608 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2174 4615 809 Size: 507 858 2441 1802 Fragments arranged by size: 2441 1802 858 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2721 3055 3944 Size: 54 1425 50 275 18 891 334 889 Cuts at: 3944 5573 8 Size: 1629 43 Fragments arranged by size: 1629 1425 891 889 334 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3055 1537 Size: 275 1243 4090 Fragments arranged by size: 4090 1243 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2271 2537 3538 1353 Size: 918 266 1001 3423 Fragments arranged by size: 3423 1001 918 266 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4195 4569 4715 387 Size: 1064 138 2606 374 146 1280 Fragments arranged by size: 2606 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2011 2011 Size: 5608 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2295 2337 2561 2860 3671 4268 5055 Size: 393 663 42 224 299 811 597 787 Cuts at: 5055 5323 1239 Size: 268 1524 Fragments arranged by size: 1524 811 787 663 597 393 299 268 224 42 EcoNI CCTnn'n_nnAGG Cuts at: 2030 2030 Size: 5608 EcoO109I rG'GnC_Cy Cuts at: 2413 2696 2413 Size: 283 5325 EcoRI G'AATT_C Cuts at: 2917 3295 2917 Size: 378 5230 FspI TGC'GCA Cuts at: 4114 4114 Size: 5608 HaeII r_GCGC'y Cuts at: 1678 2151 4755 1678 Size: 473 2604 2531 Fragments arranged by size: 2604 2531 473 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2730 2762 2853 2885 4249 Size: 32 135 32 1158 32 91 32 1364 Cuts at: 4249 4281 4323 4355 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1364 1158 135 91 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2952 3198 669 Size: 988 281 1014 246 3079 Fragments arranged by size: 3079 1014 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5608 HpaI GTT'AAC Cuts at: 3198 3198 Size: 5608 KpnI G_GTAC'C Cuts at: 2933 2933 Size: 5608 MfeI C'AATT_G Cuts at: 3207 3207 Size: 5608 MluI A'CGCG_T Cuts at: 5484 5484 Size: 5608 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4722 4906 1305 Size: 3417 184 2007 Fragments arranged by size: 3417 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3786 Fragments arranged by size: 3786 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2468 3566 3925 4084 518 Size: 648 1302 1098 359 159 2042 Fragments arranged by size: 2042 1302 1098 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2127 2687 3024 3701 4849 5094 1606 Size: 521 560 337 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 560 521 337 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4117 Fragments arranged by size: 4117 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5608 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5608 NotI GC'GGCC_GC Cuts at: 3055 3055 Size: 5608 NspI r_CATG'y Cuts at: 1132 1229 2195 2966 4511 1132 Size: 97 966 771 1545 2229 Fragments arranged by size: 2229 1545 966 771 97 PasI CC'CwG_GG Cuts at: 1348 2678 1348 Size: 1330 4278 PciI A'CATG_T Cuts at: 4507 4507 Size: 5608 PflMI CCAn_nnn'nTGG Cuts at: 1108 2143 2206 1108 Size: 1035 63 4510 Fragments arranged by size: 4510 1035 63 PmeI GTTT'AAAC Cuts at: 2912 2912 Size: 5608 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3677 3709 5190 5222 3677 Size: 32 1481 32 4063 Fragments arranged by size: 4063 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5608 PsiI TTA'TAA Cuts at: 3178 3178 Size: 5608 PstI C_TGCA'G Cuts at: 830 2960 830 Size: 2130 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5608 PvuII CAG'CTG Cuts at: 2687 2687 Size: 5608 SacI G_AGCT'C Cuts at: 2013 2013 Size: 5608 SalI G'TCGA_C Cuts at: 1936 2950 1936 Size: 1014 4594 SapI GCTCTTCn'nnn_ Cuts at: 2537 2537 Size: 5608 SbfI CC_TGCA'GG Cuts at: 2960 2960 Size: 5608 ScaI AGT'ACT Cuts at: 3856 5568 3856 Size: 1712 3896 SexAI A'CCwGG_T Cuts at: 2202 2202 Size: 5608 SfcI C'TryA_G Cuts at: 826 1045 2956 4091 4772 4963 826 Size: 219 1911 1135 681 191 1471 Fragments arranged by size: 1911 1471 1135 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2720 2720 Size: 5608 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5608 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5608 SmaI CCC'GGG Cuts at: 1872 2935 1872 Size: 1063 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2345 3712 4613 4875 820 Size: 197 926 402 1367 901 262 1553 Fragments arranged by size: 1553 1367 926 901 402 262 197 SnaBI TAC'GTA Cuts at: 493 2116 3399 493 Size: 1623 1283 2702 Fragments arranged by size: 2702 1623 1283 SpeI A'CTAG_T Cuts at: 152 152 Size: 5608 SphI G_CATG'C Cuts at: 1132 2966 1132 Size: 1834 3774 SspI AAT'ATT Cuts at: 5 52 3532 5 Size: 47 3480 2081 Fragments arranged by size: 3480 2081 47 StuI AGG'CCT Cuts at: 1517 2370 1517 Size: 853 4755 StyI C'CwwG_G Cuts at: 513 1065 2004 2088 2215 2365 2709 3047 513 Size: 552 939 84 127 150 344 338 3074 Fragments arranged by size: 3074 939 552 344 338 150 127 84 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2522 2749 2780 3635 3652 3805 3990 Size: 1255 942 227 31 855 17 153 185 Cuts at: 3990 4435 325 Size: 445 1498 Fragments arranged by size: 1498 1255 942 855 445 227 185 153 31 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3854 5566 96 Size: 274 80 33 51 1443 1877 1712 138 Fragments arranged by size: 1877 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5568 5600 5568 Size: 32 5576 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5608 XbaI T'CTAG_A Cuts at: 2944 2944 Size: 5608 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2851 2851 Size: 5608 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5608 XmaI C'CCGG_G Cuts at: 1870 2933 1870 Size: 1063 4545 XmnI GAAnn'nnTTC Cuts at: 3737 3737 Size: 5608 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3416 276 Size: 53 83 186 936 1882 2468 Fragments arranged by size: 2468 1882 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SfoI SrfI SwaI