(Circular) (Six-base) MAPSORT of: pFN21AB8647.seq Check: 7413 from: 1 to: 7177 pFN21AB8647 7177 bp With 182 enzymes: SgfI * August 16, 2010 14:42 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2432 2730 2432 Size: 298 6879 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4987 278 Size: 53 83 186 936 3451 2468 Fragments arranged by size: 3451 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3772 4428 4520 1937 Size: 1835 656 92 4594 Fragments arranged by size: 4594 1835 656 92 Acc65I G'GTAC_C Cuts at: 4498 4498 Size: 7177 AclI AA'CG_TT Cuts at: 1563 5304 5677 1563 Size: 3741 373 3063 Fragments arranged by size: 3741 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2702 5240 6624 6892 1632 Size: 1070 2538 1384 268 1917 Fragments arranged by size: 2538 1917 1384 1070 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6980 AflIII A'CryG_T Cuts at: 1203 4175 6076 7053 1203 Size: 2972 1901 977 1327 Fragments arranged by size: 2972 1901 1327 977 AgeI A'CCGG_T Cuts at: 6008 6008 Size: 7177 AhdI GACnn_n'nnGTC Cuts at: 3386 5906 3386 Size: 2520 4657 AleI CACnn'nnGTG Cuts at: 1166 2840 3780 4445 1166 Size: 1674 940 665 3898 Fragments arranged by size: 3898 1674 940 665 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3509 4217 1184 Size: 2325 708 4144 Fragments arranged by size: 4144 2325 708 AlwNI CAG_nnn'CTG Cuts at: 2497 4237 4920 6492 2497 Size: 1740 683 1572 3182 Fragments arranged by size: 3182 1740 1572 683 ApaI G_GGCC'C Cuts at: 2732 2817 3306 3695 3793 2732 Size: 85 489 389 98 6116 Fragments arranged by size: 6116 489 389 98 85 ApaLI G'TGCA_C Cuts at: 3166 3317 5234 6390 3166 Size: 151 1917 1156 3953 Fragments arranged by size: 3953 1917 1156 151 ApoI r'AATT_y Cuts at: 1493 2623 4486 4712 4864 6973 1493 Size: 1130 1863 226 152 2109 1697 Fragments arranged by size: 2109 1863 1697 1130 226 152 AseI AT'TA_AT Cuts at: 160 1032 5731 160 Size: 872 4699 1606 Fragments arranged by size: 4699 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7177 AvaI C'yCGr_G Cuts at: 1870 1943 2118 2453 2480 2573 4502 1870 Size: 73 175 335 27 93 1929 4545 Fragments arranged by size: 4545 1929 335 175 93 73 27 AvrII C'CTAG_G Cuts at: 2203 2203 Size: 7177 BamHI G'GATC_C Cuts at: 3240 4458 4507 4874 3240 Size: 1218 49 367 5543 Fragments arranged by size: 5543 1218 367 49 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2137 2718 3081 3091 3562 Size: 325 217 627 350 581 363 10 471 Cuts at: 3562 4100 4115 4498 5953 618 Size: 538 15 383 1455 1842 Fragments arranged by size: 1842 1455 627 581 538 471 383 363 350 325 217 15 10 BanII G_rGCy'C Cuts at: 1391 2352 2480 2732 2817 2868 3306 3695 3793 Size: 961 128 252 85 51 438 389 98 Cuts at: 3793 4071 4496 1391 Size: 278 425 4072 Fragments arranged by size: 4072 961 438 425 389 278 252 128 98 85 51 BbeI G_GCGC'C Cuts at: 4104 4119 4104 Size: 15 7162 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2962 928 Size: 971 1063 5143 Fragments arranged by size: 5143 1063 971 BbvCI CC'TCA_GC Cuts at: 3029 3029 Size: 7177 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 5368 5402 1903 Size: 34 50 34 3347 34 3678 Fragments arranged by size: 3678 3347 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5069 6279 5069 Size: 1210 5967 BclI T'GATC_A Cuts at: 1548 4449 1548 Size: 2901 4276 BfrBI ATG'CAT Cuts at: 3486 3486 Size: 7177 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2900 3423 3690 Size: 107 122 71 631 605 1228 523 267 Cuts at: 3690 4124 5788 136 Size: 434 1664 1525 Fragments arranged by size: 1664 1525 1228 631 605 523 434 267 122 107 71 BglII A'GATC_T Cuts at: 3288 7172 3288 Size: 3884 3293 BlpI GC'TnA_GC Cuts at: 2474 4594 2474 Size: 2120 5057 Bme1580I G_kGCm'C Cuts at: 1790 2142 2732 2817 3096 3170 3306 3321 3695 Size: 352 590 85 279 74 136 15 374 Cuts at: 3695 3793 5238 6394 1790 Size: 98 1445 1156 2573 Fragments arranged by size: 2573 1445 1156 590 374 352 279 136 98 85 74 15 BmgBI CAC'GTC Cuts at: 1318 2806 3982 1318 Size: 1488 1176 4513 Fragments arranged by size: 4513 1488 1176 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4908 5866 442 Size: 416 53 83 399 24 3491 958 1753 Fragments arranged by size: 3491 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7177 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3380 3380 Size: 7177 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2371 2689 2780 2840 3375 3671 3682 3743 Size: 1132 318 91 60 535 296 11 61 Cuts at: 3743 3930 5837 1239 Size: 187 1907 2579 Fragments arranged by size: 2579 1907 1132 535 318 296 187 91 61 60 11 Bpu10I CC'TnA_GC Cuts at: 3029 3029 Size: 7177 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2327 5302 6167 6465 2327 Size: 2975 865 298 3039 Fragments arranged by size: 3039 2975 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5840 882 Size: 617 4341 2219 Fragments arranged by size: 4341 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2656 4968 493 Size: 711 1452 2312 2702 Fragments arranged by size: 2702 2312 1452 711 BsaBI GATnn'nnATC Cuts at: 3269 7171 3269 Size: 3902 3275 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2885 3024 4101 4116 Size: 53 83 186 936 1352 139 1077 15 Cuts at: 4116 4268 4425 4984 5366 6001 275 Size: 152 157 559 382 635 1451 Fragments arranged by size: 1451 1352 1077 936 635 559 382 186 157 152 139 83 53 15 BsaWI w'CCGG_w Cuts at: 3597 5610 6008 6282 6429 6840 3597 Size: 2013 398 274 147 411 3934 Fragments arranged by size: 3934 2013 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2369 2375 2399 2405 2501 Size: 30 165 30 966 6 24 6 96 Cuts at: 2501 2531 2687 2717 3190 3220 3266 3296 1178 Size: 30 156 30 473 30 46 30 5059 Fragments arranged by size: 5059 966 473 165 156 96 46 30 30 30 30 30 30 24 6 6 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2522 3157 3680 1186 Size: 1336 635 523 4683 Fragments arranged by size: 4683 1336 635 523 BseYI C'CCAG_C Cuts at: 2632 3047 3807 4095 4389 6380 6876 2632 Size: 415 760 288 294 1991 496 2933 Fragments arranged by size: 2933 1991 760 496 415 294 288 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2510 2909 2978 3929 4061 4238 4261 Size: 573 774 399 69 951 132 177 23 Cuts at: 4261 1163 Size: 4079 Fragments arranged by size: 4079 951 774 573 399 177 132 69 23 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2907 3261 4023 4627 5388 6416 Size: 275 186 906 354 762 604 761 1028 Cuts at: 6416 1540 Size: 2301 Fragments arranged by size: 2301 1028 906 762 761 604 354 275 186 BsiHKAI G_wGCw'C Cuts at: 2352 2460 2952 3170 3321 3672 5238 5323 6394 Size: 108 492 218 151 351 1566 85 1071 Cuts at: 6394 2352 Size: 3135 Fragments arranged by size: 3135 1566 1071 492 351 218 151 108 85 BsmI GAATG_Cn' Cuts at: 4688 4781 4688 Size: 93 7084 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2142 2352 2460 2480 2732 2817 2868 Size: 399 352 210 108 20 252 85 51 Cuts at: 2868 2952 3096 3170 3306 3321 3672 3695 3793 Size: 84 144 74 136 15 351 23 98 Cuts at: 3793 4071 4496 5238 5323 6394 1391 Size: 278 425 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 425 399 352 351 278 252 210 144 136 108 98 85 85 84 74 51 23 20 15 BspEI T'CCGG_A Cuts at: 6840 6840 Size: 7177 BspHI T'CATG_A Cuts at: 5066 6796 5066 Size: 1730 5447 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2432 2730 4532 844 Size: 1588 298 1802 3489 Fragments arranged by size: 3489 1802 1588 298 BsrBI CCG'CTC Cuts at: 1394 1823 5064 1394 Size: 429 3241 3507 Fragments arranged by size: 3507 3241 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5672 5846 65 Size: 1183 4424 174 1396 Fragments arranged by size: 4424 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2463 2962 4286 4407 5821 6008 1700 Size: 110 653 499 1324 121 1414 187 2869 Fragments arranged by size: 2869 1414 1324 653 499 187 121 110 BsrGI T'GTAC_A Cuts at: 96 3330 96 Size: 3234 3943 BssHII G'CGCG_C Cuts at: 1925 4049 1925 Size: 2124 5053 BssSI C'ACGA_G Cuts at: 2165 5237 6249 2165 Size: 3072 1012 3093 Fragments arranged by size: 3093 3072 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2497 794 Size: 1703 5474 BstBI TT'CG_AA Cuts at: 5981 5981 Size: 7177 BstEII G'GTnAC_C Cuts at: 1184 4471 1184 Size: 3287 3890 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2033 3427 2011 Size: 22 1394 5761 Fragments arranged by size: 5761 1394 22 BstYI r'GATC_y Cuts at: 1972 2281 3240 3288 4458 4507 4874 5259 5276 Size: 309 959 48 1170 49 367 385 17 Cuts at: 5276 6728 6814 6826 7172 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1170 959 385 367 346 309 86 49 48 17 12 BtgI C'CryG_G Cuts at: 513 1065 2010 2868 3246 3255 3743 3855 513 Size: 552 945 858 378 9 488 112 3835 Fragments arranged by size: 3835 945 858 552 488 378 112 9 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3781 487 Size: 683 56 2555 3883 Fragments arranged by size: 3883 2555 683 56 BtsI GCAGTG_nn' Cuts at: 2320 2495 3070 3491 3554 3632 3826 4078 4693 Size: 175 575 421 63 78 194 252 615 Cuts at: 4693 5487 5507 2320 Size: 794 20 3990 Fragments arranged by size: 3990 794 615 575 421 252 194 175 78 63 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7177 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7142 DraI TTT'AAA Cuts at: 4481 4481 Size: 7177 DraIII CAC_nnn'GTG Cuts at: 4009 4358 4009 Size: 349 6828 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6184 809 Size: 507 4868 1802 Fragments arranged by size: 4868 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2013 2793 2808 Size: 54 1425 50 275 18 183 780 15 Cuts at: 2808 3252 3258 3740 4020 4360 4405 4624 5513 Size: 444 6 482 280 340 45 219 889 Cuts at: 5513 7142 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 780 482 444 340 280 275 219 183 54 50 45 43 18 15 6 EagI C'GGCC_G Cuts at: 1537 1812 3258 4020 4624 1537 Size: 275 1446 762 604 4090 Fragments arranged by size: 4090 1446 762 604 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2819 3537 3954 5107 1353 Size: 1466 718 417 1153 3423 Fragments arranged by size: 3423 1466 1153 718 417 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2120 2231 4142 5764 6138 6284 Size: 1064 138 531 111 1911 1622 374 146 Cuts at: 6284 387 Size: 1280 Fragments arranged by size: 1911 1622 1280 1064 531 374 146 138 111 EcoICRI GAG'CTC Cuts at: 2350 2350 Size: 7177 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2371 2689 2702 2780 2840 3375 3671 Size: 393 739 318 13 78 60 535 296 Cuts at: 3671 3682 3743 3930 5240 5837 6624 6892 1239 Size: 11 61 187 1310 597 787 268 1524 Fragments arranged by size: 1524 1310 787 739 597 535 393 318 296 268 187 78 61 60 13 11 EcoNI CCTnn'n_nnAGG Cuts at: 3881 3881 Size: 7177 EcoO109I rG'GnC_Cy Cuts at: 2020 2414 2578 2671 2687 2728 2729 2737 2814 Size: 394 164 93 16 41 1 8 77 Cuts at: 2814 2920 3691 3692 3789 3790 4234 4393 2020 Size: 106 771 1 97 1 444 159 4804 Fragments arranged by size: 4804 771 444 394 164 159 106 97 93 77 41 16 8 1 1 1 EcoRI G'AATT_C Cuts at: 2623 4486 4864 2623 Size: 1863 378 4936 Fragments arranged by size: 4936 1863 378 FseI GG_CCGG'CC Cuts at: 4411 4411 Size: 7177 FspI TGC'GCA Cuts at: 3215 5683 3215 Size: 2468 4709 HaeII r_GCGC'y Cuts at: 1678 3989 4104 4119 6324 1678 Size: 2311 115 15 2205 2531 Fragments arranged by size: 2531 2311 2205 115 15 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2501 2533 3372 3404 5818 Size: 32 135 32 929 32 839 32 2414 Cuts at: 5818 5850 5892 5924 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2414 929 839 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2527 3773 4521 4767 669 Size: 988 281 589 1246 748 246 3079 Fragments arranged by size: 3079 1246 988 748 589 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7177 HpaI GTT'AAC Cuts at: 4767 4767 Size: 7177 KasI G'GCGC_C Cuts at: 4100 4115 4100 Size: 15 7162 KpnI G_GTAC'C Cuts at: 4502 4502 Size: 7177 MfeI C'AATT_G Cuts at: 4776 4776 Size: 7177 MluI A'CGCG_T Cuts at: 7053 7053 Size: 7177 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3321 6291 6475 1305 Size: 2016 2970 184 2007 Fragments arranged by size: 2970 2016 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2795 3254 3742 10 Size: 54 1425 343 963 459 488 3445 Fragments arranged by size: 3445 1425 963 488 459 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2840 3463 3780 4445 5135 5494 Size: 648 843 831 623 317 665 690 359 Cuts at: 5494 5653 518 Size: 159 2042 Fragments arranged by size: 2042 843 831 690 665 648 623 359 317 159 MspA1I CmG'CkG Cuts at: 1606 2709 3807 3821 4339 4365 4389 4455 4593 Size: 1103 1098 14 518 26 24 66 138 Cuts at: 4593 5270 6418 6663 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 1103 1098 677 518 245 138 66 26 24 14 NaeI GCC'GGC Cuts at: 2465 4288 4409 2465 Size: 1823 121 5233 Fragments arranged by size: 5233 1823 121 NarI GG'CG_CC Cuts at: 4101 4116 4101 Size: 15 7162 NcoI C'CATG_G Cuts at: 513 1065 3743 513 Size: 552 2678 3947 Fragments arranged by size: 3947 2678 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7177 NgoMIV G'CCGG_C Cuts at: 2463 4286 4407 2463 Size: 1823 121 5233 Fragments arranged by size: 5233 1823 121 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7177 NotI GC'GGCC_GC Cuts at: 4624 4624 Size: 7177 NsiI A_TGCA'T Cuts at: 3488 3488 Size: 7177 NspI r_CATG'y Cuts at: 1132 1229 3486 4179 4279 4535 6080 1132 Size: 97 2257 693 100 256 1545 2229 Fragments arranged by size: 2257 2229 1545 693 256 100 97 PasI CC'CwG_GG Cuts at: 1348 3019 3172 1348 Size: 1671 153 5353 Fragments arranged by size: 5353 1671 153 PciI A'CATG_T Cuts at: 4175 6076 4175 Size: 1901 5276 PflMI CCAn_nnn'nTGG Cuts at: 1108 4009 4468 1108 Size: 2901 459 3817 Fragments arranged by size: 3817 2901 459 PfoI T'CCnGG_A Cuts at: 2589 3834 2589 Size: 1245 5932 PmeI GTTT'AAAC Cuts at: 4481 4481 Size: 7177 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2069 2101 5246 5278 6759 6791 2069 Size: 32 3145 32 1481 32 2455 Fragments arranged by size: 3145 2455 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2414 2671 2737 4234 2414 Size: 257 66 1497 5357 Fragments arranged by size: 5357 1497 257 66 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7177 PsiI TTA'TAA Cuts at: 4747 4747 Size: 7177 PspOMI G'GGCC_C Cuts at: 2728 2813 3302 3691 3789 2728 Size: 85 489 389 98 6116 Fragments arranged by size: 6116 489 389 98 85 PstI C_TGCA'G Cuts at: 830 2727 2855 3225 3650 3996 4206 4529 830 Size: 1897 128 370 425 346 210 323 3478 Fragments arranged by size: 3478 1897 425 370 346 323 210 128 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7177 PvuII CAG'CTG Cuts at: 2709 3821 4389 4455 2709 Size: 1112 568 66 5431 Fragments arranged by size: 5431 1112 568 66 SacI G_AGCT'C Cuts at: 2352 2352 Size: 7177 SalI G'TCGA_C Cuts at: 1936 3771 4519 1936 Size: 1835 748 4594 Fragments arranged by size: 4594 1835 748 SanDI GG'GwC_CC Cuts at: 2414 2414 Size: 7177 SapI GCTCTTCn'nnn_ Cuts at: 3537 3537 Size: 7177 SbfI CC_TGCA'GG Cuts at: 2727 4529 2727 Size: 1802 5375 ScaI AGT'ACT Cuts at: 5425 7137 5425 Size: 1712 5465 SexAI A'CCwGG_T Cuts at: 4260 4260 Size: 7177 SfcI C'TryA_G Cuts at: 826 1045 2548 2647 2723 2851 3221 3646 3992 Size: 219 1503 99 76 128 370 425 346 Cuts at: 3992 4202 4525 5660 6341 6532 826 Size: 210 323 1135 681 191 1471 Fragments arranged by size: 1503 1471 1135 681 425 370 346 323 219 210 191 128 99 76 SfiI GGCCn_nnn'nGGCC Cuts at: 3690 3690 Size: 7177 SfoI GGC'GCC Cuts at: 4102 4117 4102 Size: 15 7162 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7177 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7177 SmaI CCC'GGG Cuts at: 1872 2120 4504 1872 Size: 248 2384 4545 Fragments arranged by size: 4545 2384 248 SmlI C'TyrA_G Cuts at: 820 1017 1943 2342 5281 6182 6444 820 Size: 197 926 399 2939 901 262 1553 Fragments arranged by size: 2939 1553 926 901 399 262 197 SnaBI TAC'GTA Cuts at: 493 4968 493 Size: 4475 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7177 SphI G_CATG'C Cuts at: 1132 4279 4535 1132 Size: 3147 256 3774 Fragments arranged by size: 3774 3147 256 SspI AAT'ATT Cuts at: 5 52 5101 5 Size: 47 5049 2081 Fragments arranged by size: 5049 2081 47 StuI AGG'CCT Cuts at: 1517 2093 2978 1517 Size: 576 885 5716 Fragments arranged by size: 5716 885 576 StyI C'CwwG_G Cuts at: 513 1065 2203 2733 3498 3743 4162 4616 513 Size: 552 1138 530 765 245 419 454 3074 Fragments arranged by size: 3074 1138 765 552 530 454 419 245 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2802 3185 3799 5204 5221 5374 5559 Size: 1255 1222 383 614 1405 17 153 185 Cuts at: 5559 6004 325 Size: 445 1498 Fragments arranged by size: 1498 1405 1255 1222 614 445 383 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2238 2257 3330 Size: 274 80 33 51 1443 261 19 1073 Cuts at: 3330 5423 7135 96 Size: 2093 1712 138 Fragments arranged by size: 2093 1712 1443 1073 274 261 138 80 51 33 19 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2687 2719 3190 3222 7137 7169 2687 Size: 32 471 32 3915 32 2695 Fragments arranged by size: 3915 2695 471 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7177 XbaI T'CTAG_A Cuts at: 4513 4513 Size: 7177 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2365 2803 2365 Size: 438 6739 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7177 XmaI C'CCGG_G Cuts at: 1870 2118 4502 1870 Size: 248 2384 4545 Fragments arranged by size: 4545 2384 248 XmnI GAAnn'nnTTC Cuts at: 5306 5306 Size: 7177 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4985 276 Size: 53 83 186 936 3451 2468 Fragments arranged by size: 3451 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI BaeI BsiWI BsmBI BstZ17I Bsu36I EcoRV FalI FspAI NruI PacI PmlI PspXI PsrI RsrII SacII SrfI SwaI