(Circular) (Six-base) MAPSORT of: pFN21AE0196.seq Check: 6420 from: 1 to: 5953 pFN21AE0196 5953 bp With 182 enzymes: SgfI * October 20, 2009 10:45 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2646 2646 Size: 5953 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3763 278 Size: 53 83 186 936 2227 2468 Fragments arranged by size: 2468 2227 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3296 1937 Size: 1359 4594 Acc65I G'GTAC_C Cuts at: 3274 3274 Size: 5953 AclI AA'CG_TT Cuts at: 1563 4080 4453 1563 Size: 2517 373 3063 Fragments arranged by size: 3063 2517 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2856 2901 4016 5400 5668 1632 Size: 1224 45 1115 1384 268 1917 Fragments arranged by size: 1917 1384 1224 1115 268 45 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5756 AflIII A'CryG_T Cuts at: 1203 4852 5829 1203 Size: 3649 977 1327 Fragments arranged by size: 3649 1327 977 AgeI A'CCGG_T Cuts at: 4784 4784 Size: 5953 AhdI GACnn_n'nnGTC Cuts at: 4682 4682 Size: 5953 AleI CACnn'nnGTG Cuts at: 1166 2631 1166 Size: 1465 4488 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2695 1184 Size: 1511 4442 AlwNI CAG_nnn'CTG Cuts at: 2453 3696 5268 2453 Size: 1243 1572 3138 Fragments arranged by size: 3138 1572 1243 ApaLI G'TGCA_C Cuts at: 4010 5166 4010 Size: 1156 4797 ApoI r'AATT_y Cuts at: 1493 2263 2351 3003 3262 3488 3640 5749 1493 Size: 770 88 652 259 226 152 2109 1697 Fragments arranged by size: 2109 1697 770 652 259 226 152 88 AseI AT'TA_AT Cuts at: 160 1032 4507 160 Size: 872 3475 1606 Fragments arranged by size: 3475 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5953 AvaI C'yCGr_G Cuts at: 1870 1943 3207 3278 1870 Size: 73 1264 71 4545 Fragments arranged by size: 4545 1264 73 71 BamHI G'GATC_C Cuts at: 3283 3650 3283 Size: 367 5586 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3274 4729 618 Size: 325 217 627 1487 1455 1842 Fragments arranged by size: 1842 1487 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3272 1391 Size: 1881 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2096 2138 2867 928 Size: 971 197 42 729 4014 Fragments arranged by size: 4014 971 729 197 42 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4144 4178 1903 Size: 34 2207 34 3678 Fragments arranged by size: 3678 2207 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3845 5055 3845 Size: 1210 4743 BclI T'GATC_A Cuts at: 1548 2646 1548 Size: 1098 4855 BfrBI ATG'CAT Cuts at: 2255 3023 3086 2255 Size: 768 63 5122 Fragments arranged by size: 5122 768 63 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4564 136 Size: 107 122 71 631 605 2892 1525 Fragments arranged by size: 2892 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2033 5948 2033 Size: 3915 2038 BlpI GC'TnA_GC Cuts at: 3370 3370 Size: 5953 Bme1580I G_kGCm'C Cuts at: 1790 4014 5170 1790 Size: 2224 1156 2573 Fragments arranged by size: 2573 2224 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5953 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3684 4642 442 Size: 416 53 83 399 24 2267 958 1753 Fragments arranged by size: 2267 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5953 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2187 2349 2600 4613 1239 Size: 948 162 251 2013 2579 Fragments arranged by size: 2579 2013 948 251 162 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4078 4943 5241 4078 Size: 865 298 4790 Fragments arranged by size: 4790 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4616 882 Size: 617 3117 2219 Fragments arranged by size: 3117 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3744 493 Size: 711 2540 2702 Fragments arranged by size: 2702 2540 711 BsaBI GATnn'nnATC Cuts at: 5947 5947 Size: 5953 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3760 4142 4777 275 Size: 53 83 186 936 2227 382 635 1451 Fragments arranged by size: 2227 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4386 4784 5058 5205 5616 4386 Size: 398 274 147 411 4723 Fragments arranged by size: 4723 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2718 2748 1178 Size: 30 165 30 1315 30 4383 Fragments arranged by size: 4383 1315 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5953 BseYI C'CCAG_C Cuts at: 3162 5156 5652 3162 Size: 1994 496 3463 Fragments arranged by size: 3463 1994 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2357 2846 1163 Size: 573 621 489 4270 Fragments arranged by size: 4270 621 573 489 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3403 4164 5192 1540 Size: 275 186 1402 761 1028 2301 Fragments arranged by size: 2301 1402 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4014 4099 5170 4014 Size: 85 1071 4797 Fragments arranged by size: 4797 1071 85 BsmI GAATG_Cn' Cuts at: 2449 3464 3557 2449 Size: 1015 93 4845 Fragments arranged by size: 4845 1015 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3272 4014 4099 5170 1391 Size: 399 1482 742 85 1071 2174 Fragments arranged by size: 2174 1482 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5616 5616 Size: 5953 BspHI T'CATG_A Cuts at: 2779 2806 3842 5572 2779 Size: 27 1036 1730 3160 Fragments arranged by size: 3160 1730 1036 27 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2646 3308 844 Size: 1802 662 3489 Fragments arranged by size: 3489 1802 662 BsrBI CCG'CTC Cuts at: 1394 1823 3840 1394 Size: 429 2017 3507 Fragments arranged by size: 3507 2017 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2618 3016 4448 4622 65 Size: 1183 1370 398 1432 174 1396 Fragments arranged by size: 1432 1396 1370 1183 398 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4597 4784 1700 Size: 110 2787 187 2869 Fragments arranged by size: 2869 2787 187 110 BsrGI T'GTAC_A Cuts at: 96 2007 96 Size: 1911 4042 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5953 BssSI C'ACGA_G Cuts at: 4013 5025 4013 Size: 1012 4941 BstAPI GCAn_nnn'nTGC Cuts at: 794 2453 794 Size: 1659 4294 BstBI TT'CG_AA Cuts at: 4757 4757 Size: 5953 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5953 BstYI r'GATC_y Cuts at: 1972 2033 3283 3650 4035 4052 5504 5590 5602 Size: 61 1250 367 385 17 1452 86 12 Cuts at: 5602 5948 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1250 385 367 346 86 61 17 12 BtgI C'CryG_G Cuts at: 513 1065 2594 513 Size: 552 1529 3872 Fragments arranged by size: 3872 1529 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5214 Fragments arranged by size: 5214 683 56 BtsI GCAGTG_nn' Cuts at: 2677 3469 4263 4283 2677 Size: 792 794 20 4347 Fragments arranged by size: 4347 794 792 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5953 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2497 2532 529 Size: 35 1933 35 3950 Fragments arranged by size: 3950 1933 35 35 DraI TTT'AAA Cuts at: 3257 3257 Size: 5953 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4960 809 Size: 507 3644 1802 Fragments arranged by size: 3644 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2039 2591 3400 Size: 54 1425 50 275 18 209 552 809 Cuts at: 3400 4289 5918 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 809 552 275 209 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3400 1537 Size: 275 1588 4090 Fragments arranged by size: 4090 1588 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3883 1353 Size: 2530 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4540 4914 5060 387 Size: 1064 138 2951 374 146 1280 Fragments arranged by size: 2951 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2187 2349 2600 2856 2901 4016 4613 Size: 393 555 162 251 256 45 1115 597 Cuts at: 4613 5400 5668 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1115 787 597 555 393 268 256 251 162 45 EcoRI G'AATT_C Cuts at: 3262 3640 3262 Size: 378 5575 FspI TGC'GCA Cuts at: 4459 4459 Size: 5953 HaeII r_GCGC'y Cuts at: 1678 5100 1678 Size: 3422 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3205 3237 4594 4626 4668 Size: 32 135 32 1633 32 1357 32 42 Cuts at: 4668 4700 1373 Size: 32 2626 Fragments arranged by size: 2626 1633 1357 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2719 3297 3543 669 Size: 988 281 781 578 246 3079 Fragments arranged by size: 3079 988 781 578 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5953 HpaI GTT'AAC Cuts at: 3543 3543 Size: 5953 KpnI G_GTAC'C Cuts at: 3278 3278 Size: 5953 MfeI C'AATT_G Cuts at: 3226 3552 3226 Size: 326 5627 MluI A'CGCG_T Cuts at: 5829 5829 Size: 5953 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3130 5067 5251 1305 Size: 1825 1937 184 2007 Fragments arranged by size: 2007 1937 1825 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2041 2593 10 Size: 54 1425 343 209 552 3370 Fragments arranged by size: 3370 1425 552 343 209 54 MslI CAynn'nnrTG Cuts at: 518 1166 2593 2631 3911 4270 4429 518 Size: 648 1427 38 1280 359 159 2042 Fragments arranged by size: 2042 1427 1280 648 359 159 38 MspA1I CmG'CkG Cuts at: 1606 2347 3369 4046 5194 5439 1606 Size: 741 1022 677 1148 245 2120 Fragments arranged by size: 2120 1148 1022 741 677 245 NcoI C'CATG_G Cuts at: 513 1065 2594 513 Size: 552 1529 3872 Fragments arranged by size: 3872 1529 552 NdeI CA'TA_TG Cuts at: 387 3019 387 Size: 2632 3321 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5953 NotI GC'GGCC_GC Cuts at: 3400 3400 Size: 5953 NsiI A_TGCA'T Cuts at: 2257 3025 3088 2257 Size: 768 63 5122 Fragments arranged by size: 5122 768 63 NspI r_CATG'y Cuts at: 1132 1229 2611 3090 3311 4856 1132 Size: 97 1382 479 221 1545 2229 Fragments arranged by size: 2229 1545 1382 479 221 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5953 PciI A'CATG_T Cuts at: 4852 4852 Size: 5953 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5953 PfoI T'CCnGG_A Cuts at: 2438 2438 Size: 5953 PmeI GTTT'AAAC Cuts at: 3257 3257 Size: 5953 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4022 4054 5535 5567 4022 Size: 32 1481 32 4408 Fragments arranged by size: 4408 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5953 PsiI TTA'TAA Cuts at: 3523 3523 Size: 5953 PspXI vC'TCGA_Gb Cuts at: 3207 3207 Size: 5953 PstI C_TGCA'G Cuts at: 830 2674 3305 830 Size: 1844 631 3478 Fragments arranged by size: 3478 1844 631 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5953 PvuII CAG'CTG Cuts at: 2347 2347 Size: 5953 SalI G'TCGA_C Cuts at: 1936 3295 1936 Size: 1359 4594 SbfI CC_TGCA'GG Cuts at: 3305 3305 Size: 5953 ScaI AGT'ACT Cuts at: 4201 5913 4201 Size: 1712 4241 SfcI C'TryA_G Cuts at: 826 1045 2670 3301 4436 5117 5308 826 Size: 219 1625 631 1135 681 191 1471 Fragments arranged by size: 1625 1471 1135 681 631 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5953 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5953 SmaI CCC'GGG Cuts at: 1872 3280 1872 Size: 1408 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3207 4057 4958 5220 820 Size: 197 926 1264 850 901 262 1553 Fragments arranged by size: 1553 1264 926 901 850 262 197 SnaBI TAC'GTA Cuts at: 493 3744 493 Size: 3251 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5953 SphI G_CATG'C Cuts at: 1132 2611 3311 1132 Size: 1479 700 3774 Fragments arranged by size: 3774 1479 700 SspI AAT'ATT Cuts at: 5 52 3877 5 Size: 47 3825 2081 Fragments arranged by size: 3825 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5953 StyI C'CwwG_G Cuts at: 513 1065 2594 3392 513 Size: 552 1529 798 3074 Fragments arranged by size: 3074 1529 798 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3980 3997 4150 4335 4780 325 Size: 1255 2400 17 153 185 445 1498 Fragments arranged by size: 2400 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2007 2856 4199 Size: 274 80 33 51 1443 30 849 1343 Cuts at: 4199 5911 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1343 849 274 138 80 51 33 30 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2745 2777 5913 5945 2745 Size: 32 3136 32 2753 Fragments arranged by size: 3136 2753 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5953 XbaI T'CTAG_A Cuts at: 3289 3289 Size: 5953 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2467 2467 Size: 5953 XhoI C'TCGA_G Cuts at: 1943 3207 1943 Size: 1264 4689 XmaI C'CCGG_G Cuts at: 1870 3278 1870 Size: 1408 4545 XmnI GAAnn'nnTTC Cuts at: 4082 4082 Size: 5953 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3761 276 Size: 53 83 186 936 2227 2468 Fragments arranged by size: 2468 2227 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspXI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI