(Circular) (Six-base) MAPSORT of: pFN21AE0182.seq Check: 8084 from: 1 to: 5383 pFN21AE0182 5383 bp With 182 enzymes: SgfI * October 20, 2009 10:45 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3193 278 Size: 53 83 186 936 1657 2468 Fragments arranged by size: 2468 1657 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2726 1937 Size: 789 4594 Acc65I G'GTAC_C Cuts at: 2704 2704 Size: 5383 AclI AA'CG_TT Cuts at: 1563 3510 3883 1563 Size: 1947 373 3063 Fragments arranged by size: 3063 1947 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2352 2673 3446 4830 5098 1632 Size: 720 321 773 1384 268 1917 Fragments arranged by size: 1917 1384 773 720 321 268 AfeI AGC'GCT Cuts at: 2093 2123 2093 Size: 30 5353 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5186 AflIII A'CryG_T Cuts at: 1203 4282 5259 1203 Size: 3079 977 1327 Fragments arranged by size: 3079 1327 977 AgeI A'CCGG_T Cuts at: 4214 4214 Size: 5383 AhdI GACnn_n'nnGTC Cuts at: 4112 4112 Size: 5383 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5383 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5383 AlwNI CAG_nnn'CTG Cuts at: 2572 2651 3126 4698 2572 Size: 79 475 1572 3257 Fragments arranged by size: 3257 1572 475 79 ApaLI G'TGCA_C Cuts at: 3440 4596 3440 Size: 1156 4227 ApoI r'AATT_y Cuts at: 1493 2692 2918 3070 5179 1493 Size: 1199 226 152 2109 1697 Fragments arranged by size: 2109 1697 1199 226 152 AseI AT'TA_AT Cuts at: 160 1032 3937 160 Size: 872 2905 1606 Fragments arranged by size: 2905 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5383 AvaI C'yCGr_G Cuts at: 1870 1943 2266 2708 1870 Size: 73 323 442 4545 Fragments arranged by size: 4545 442 323 73 BamHI G'GATC_C Cuts at: 2713 3080 2713 Size: 367 5016 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2156 2704 4159 618 Size: 325 217 627 369 548 1455 1842 Fragments arranged by size: 1842 1455 627 548 369 325 217 BanII G_rGCy'C Cuts at: 1391 2181 2348 2702 1391 Size: 790 167 354 4072 Fragments arranged by size: 4072 790 354 167 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2253 2369 928 Size: 971 354 116 3942 Fragments arranged by size: 3942 971 354 116 BbvCI CC'TCA_GC Cuts at: 2583 2583 Size: 5383 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2470 2504 3574 3608 1903 Size: 34 533 34 1070 34 3678 Fragments arranged by size: 3678 1070 533 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3275 4485 3275 Size: 1210 4173 BclI T'GATC_A Cuts at: 1548 1548 Size: 5383 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2646 2655 3994 Size: 107 122 71 631 605 974 9 1339 Cuts at: 3994 136 Size: 1525 Fragments arranged by size: 1525 1339 974 631 605 122 107 71 9 BglII A'GATC_T Cuts at: 5378 5378 Size: 5383 BlpI GC'TnA_GC Cuts at: 2800 2800 Size: 5383 Bme1580I G_kGCm'C Cuts at: 1790 3444 4600 1790 Size: 1654 1156 2573 Fragments arranged by size: 2573 1654 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5383 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2164 3114 4072 Size: 416 53 83 399 24 747 950 958 Cuts at: 4072 442 Size: 1753 Fragments arranged by size: 1753 958 950 747 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5383 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2258 4043 1239 Size: 1019 1785 2579 Fragments arranged by size: 2579 1785 1019 Bpu10I CC'TnA_GC Cuts at: 2583 2583 Size: 5383 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3508 4373 4671 3508 Size: 865 298 4220 Fragments arranged by size: 4220 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2103 2159 2276 4046 882 Size: 617 604 56 117 1770 2219 Fragments arranged by size: 2219 1770 617 604 117 56 BsaAI yAC'GTr Cuts at: 493 1204 3174 493 Size: 711 1970 2702 Fragments arranged by size: 2702 1970 711 BsaBI GATnn'nnATC Cuts at: 5377 5377 Size: 5383 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3190 3572 4207 275 Size: 53 83 186 936 1657 382 635 1451 Fragments arranged by size: 1657 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3816 4214 4488 4635 5046 3816 Size: 398 274 147 411 4153 Fragments arranged by size: 4153 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2156 2186 1178 Size: 30 165 30 753 30 4375 Fragments arranged by size: 4375 753 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2600 1186 Size: 1414 3969 BseYI C'CCAG_C Cuts at: 4586 5082 4586 Size: 496 4887 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4810 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2105 2833 3594 4622 1540 Size: 275 186 104 728 761 1028 2301 Fragments arranged by size: 2301 1028 761 728 275 186 104 BsiHKAI G_wGCw'C Cuts at: 3444 3529 4600 3444 Size: 85 1071 4227 Fragments arranged by size: 4227 1071 85 BsmI GAATG_Cn' Cuts at: 2533 2894 2987 2533 Size: 361 93 4929 Fragments arranged by size: 4929 361 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2181 2348 2702 3444 3529 4600 1391 Size: 399 391 167 354 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 391 354 167 85 BspEI T'CCGG_A Cuts at: 5046 5046 Size: 5383 BspHI T'CATG_A Cuts at: 3272 5002 3272 Size: 1730 3653 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2121 2738 844 Size: 1277 617 3489 Fragments arranged by size: 3489 1277 617 BsrBI CCG'CTC Cuts at: 1394 1823 3270 1394 Size: 429 1447 3507 Fragments arranged by size: 3507 1447 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2286 3878 4052 65 Size: 1183 1038 1592 174 1396 Fragments arranged by size: 1592 1396 1183 1038 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2100 4027 4214 1700 Size: 110 290 1927 187 2869 Fragments arranged by size: 2869 1927 290 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5383 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5383 BssSI C'ACGA_G Cuts at: 3443 4455 3443 Size: 1012 4371 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5383 BstBI TT'CG_AA Cuts at: 4187 4187 Size: 5383 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5383 BstXI CCAn_nnnn'nTGG Cuts at: 2053 2341 2467 2053 Size: 288 126 4969 Fragments arranged by size: 4969 288 126 BstYI r'GATC_y Cuts at: 1972 2713 3080 3465 3482 4934 5020 5032 5378 Size: 741 367 385 17 1452 86 12 346 Cuts at: 5378 1972 Size: 1977 Fragments arranged by size: 1977 1452 741 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2160 513 Size: 552 939 156 3736 Fragments arranged by size: 3736 939 552 156 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4644 Fragments arranged by size: 4644 683 56 BtsI GCAGTG_nn' Cuts at: 2281 2899 3693 3713 2281 Size: 618 794 20 3951 Fragments arranged by size: 3951 794 618 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5383 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5348 DraI TTT'AAA Cuts at: 2687 2687 Size: 5383 DraIII CAC_nnn'GTG Cuts at: 2512 2512 Size: 5383 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4390 809 Size: 507 3074 1802 Fragments arranged by size: 3074 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2040 2102 2397 Size: 54 1425 50 275 18 210 62 295 Cuts at: 2397 2830 3719 5348 8 Size: 433 889 1629 43 Fragments arranged by size: 1629 1425 889 433 295 275 210 62 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2102 2830 1537 Size: 275 290 728 4090 Fragments arranged by size: 4090 728 290 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3313 1353 Size: 1960 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3970 4344 4490 387 Size: 1064 138 2381 374 146 1280 Fragments arranged by size: 2381 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2258 2352 2673 3446 4043 4830 5098 Size: 393 626 94 321 773 597 787 268 Cuts at: 5098 1239 Size: 1524 Fragments arranged by size: 1524 787 773 626 597 393 321 268 94 EcoO109I rG'GnC_Cy Cuts at: 2648 2648 Size: 5383 EcoRI G'AATT_C Cuts at: 2692 3070 2692 Size: 378 5005 FspI TGC'GCA Cuts at: 3889 3889 Size: 5383 HaeII r_GCGC'y Cuts at: 1678 2095 2125 4530 1678 Size: 417 30 2405 2531 Fragments arranged by size: 2531 2405 417 30 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2156 2188 2431 2463 4024 Size: 32 135 32 584 32 243 32 1561 Cuts at: 4024 4056 4098 4130 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1561 584 243 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2727 2973 669 Size: 988 281 789 246 3079 Fragments arranged by size: 3079 988 789 281 246 HindIII A'AGCT_T Cuts at: 748 2324 748 Size: 1576 3807 HpaI GTT'AAC Cuts at: 2973 2973 Size: 5383 KpnI G_GTAC'C Cuts at: 2708 2708 Size: 5383 MfeI C'AATT_G Cuts at: 2679 2982 2679 Size: 303 5080 MluI A'CGCG_T Cuts at: 5259 5259 Size: 5383 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2339 2433 4497 4681 1305 Size: 1034 94 2064 184 2007 Fragments arranged by size: 2064 2007 1034 184 94 MscI TGG'CCA Cuts at: 10 64 1489 1832 2042 2399 10 Size: 54 1425 343 210 357 2994 Fragments arranged by size: 2994 1425 357 343 210 54 MslI CAynn'nnrTG Cuts at: 518 1166 2033 2339 2390 2393 3341 3700 3859 Size: 648 867 306 51 3 948 359 159 Cuts at: 3859 518 Size: 2042 Fragments arranged by size: 2042 948 867 648 359 306 159 51 3 MspA1I CmG'CkG Cuts at: 1606 2799 3476 4624 4869 1606 Size: 1193 677 1148 245 2120 Fragments arranged by size: 2120 1193 1148 677 245 NaeI GCC'GGC Cuts at: 2102 2102 Size: 5383 NcoI C'CATG_G Cuts at: 513 1065 2004 2160 513 Size: 552 939 156 3736 Fragments arranged by size: 3736 939 552 156 NdeI CA'TA_TG Cuts at: 387 387 Size: 5383 NgoMIV G'CCGG_C Cuts at: 2100 2100 Size: 5383 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5383 NotI GC'GGCC_GC Cuts at: 2830 2830 Size: 5383 NspI r_CATG'y Cuts at: 1132 1229 2741 4286 1132 Size: 97 1512 1545 2229 Fragments arranged by size: 2229 1545 1512 97 PasI CC'CwG_GG Cuts at: 1348 2415 1348 Size: 1067 4316 PciI A'CATG_T Cuts at: 4282 4282 Size: 5383 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5383 PfoI T'CCnGG_A Cuts at: 2466 2466 Size: 5383 PmeI GTTT'AAAC Cuts at: 2687 2687 Size: 5383 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3452 3484 4965 4997 3452 Size: 32 1481 32 3838 Fragments arranged by size: 3838 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5383 PsiI TTA'TAA Cuts at: 2953 2953 Size: 5383 PstI C_TGCA'G Cuts at: 830 2121 2667 2735 830 Size: 1291 546 68 3478 Fragments arranged by size: 3478 1291 546 68 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5383 SalI G'TCGA_C Cuts at: 1936 2725 1936 Size: 789 4594 SbfI CC_TGCA'GG Cuts at: 2667 2735 2667 Size: 68 5315 ScaI AGT'ACT Cuts at: 3631 5343 3631 Size: 1712 3671 SexAI A'CCwGG_T Cuts at: 2508 2508 Size: 5383 SfcI C'TryA_G Cuts at: 826 1045 2117 2663 2731 3866 4547 4738 826 Size: 219 1072 546 68 1135 681 191 1471 Fragments arranged by size: 1471 1135 1072 681 546 219 191 68 SfiI GGCCn_nnn'nGGCC Cuts at: 2646 2646 Size: 5383 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5383 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5383 SmaI CCC'GGG Cuts at: 1872 2710 1872 Size: 838 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3487 4388 4650 820 Size: 197 926 1544 901 262 1553 Fragments arranged by size: 1553 1544 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3174 493 Size: 2681 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5383 SphI G_CATG'C Cuts at: 1132 2741 1132 Size: 1609 3774 SspI AAT'ATT Cuts at: 5 52 3307 5 Size: 47 3255 2081 Fragments arranged by size: 3255 2081 47 StuI AGG'CCT Cuts at: 1517 2550 2640 1517 Size: 1033 90 4260 Fragments arranged by size: 4260 1033 90 StyI C'CwwG_G Cuts at: 513 1065 2004 2070 2160 2822 513 Size: 552 939 66 90 662 3074 Fragments arranged by size: 3074 939 662 552 90 66 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3410 3427 3580 3765 4210 325 Size: 1255 1830 17 153 185 445 1498 Fragments arranged by size: 1830 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3629 5341 96 Size: 274 80 33 51 1443 1652 1712 138 Fragments arranged by size: 1712 1652 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5343 5375 5343 Size: 32 5351 Tth111I GACn'n_nGTC Cuts at: 1316 2170 2201 1316 Size: 854 31 4498 Fragments arranged by size: 4498 854 31 XbaI T'CTAG_A Cuts at: 2719 2719 Size: 5383 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2035 2050 2011 Size: 24 15 5344 Fragments arranged by size: 5344 24 15 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5383 XmaI C'CCGG_G Cuts at: 1870 2708 1870 Size: 838 4545 XmnI GAAnn'nnTTC Cuts at: 2601 3512 2601 Size: 911 4472 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3191 276 Size: 53 83 186 936 1657 2468 Fragments arranged by size: 2468 1657 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SfoI SrfI SwaI