Restriction map for
pFN21AE0207
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# NO CUT ENZYMES ( 160 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AgeI AhdI
AluI Alw44I AlwI AocI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AviII AvrII BalI BamHI BanI
BbeI BbrPI BbsI BbvCI BcgI
BciVI BfrI BglI BglII BlnI
BlpI BpmI Bpu1102I BpuAI BsaBI
BsaHI BsaI BsaWI BseAI BseRI
BsgI BsiEI BsiHKAI BsmBI BsmFI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstXI
BstYI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraI DraIII EaeI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV FseI FspI HaeII HgaI
Hin1I HincII HindII HindIII HpaI
KasI KpnI Ksp632I KspI MamI
MboII MfeI MflI MluI MluNI
MroI MscI MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmlI SnaBI SpeI Sse8387I
SspBI SspI SwaI Tsp509I Tth111I
XbaI XcmI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccII 54 CGCG CG^CG
AfaI 132 GTAC GT^AC
AflIII 622 ACACGT A^C[GA][CT]GT
AlwNI 668 CAGTCACTG CAG[ACGT][ACGT][ACGT]^CTG
BclI 242 TGATCA T^GATCA
BfaI 215 CTAG C^TAG
BsaAI 623 CACGTA [CT]AC^GT[GA]
BsiWI 131 CGTACG C^GTACG
BsmAI 386 GTCTC GTCTC(1/5)
BsmI 342 GAATGC GAATGC(1/-1)
BspMI 247 ACCTGC ACCTGC(4/8)
BsrI 679 ACTGG ACTGG(1/-1)
BstAPI 667 GCAGTCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 54 CGCG CG^CG
DpnII 243 GATC ^GATC
DrdI 476 GACCTCTGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 500 CCATGG C^C[GA][CT]GG
EcoT14I 500 CCATGG C^C[AT][AT]GG
EcoT22I 289 ATGCAT ATGCA^T
FbaI 242 TGATCA T^GATCA
MaeI 215 CTAG C^TAG
MboI 243 GATC ^GATC
MseI 211 TTAA T^TAA
MspA1I 25 CCGCTG C[AC]G^C[GT]G
MvnI 54 CGCG CG^CG
NcoI 500 CCATGG C^CATGG
NdeII 243 GATC ^GATC
NsiI 289 ATGCAT ATGCA^T
PflMI 495 CCAACCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 188 AGGACCC [GA]G^G[AT]CC[CT]
PstI 588 CTGCAG CTGCA^G
RsaI 132 GTAC GT^AC
Sau3AI 243 GATC ^GATC
SfcI 588 CTGCAG C^T[GA][CT]AG
SmaI 100 CCCGGG CCC^GGG
StuI 108 AGGCCT AGG^CCT
StyI 500 CCATGG C^C[AT][AT]GG
TfiI 446 GAATC G^A[AT]TC
Tru9I 211 TTAA T^TAA
Van91I 495 CCAACCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 100 CCCGGG C^CCGGG
XmaI 100 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 16 enzymes) #
###################################
ApaI 76 GGGCCC GGGCC^C
97 GGGCCC
AvaI 100 CCCGGG C^[CT]CG[GA]G
157 CCCGAG
AvaII 189 GGACC G^G[AT]CC
475 GGACC
BsoBI 100 CCCGGG C^[CT]CG[GA]G
157 CCCGAG
BsrDI 526 CATTGC GCAATG(2/0)
320 GCAATG
CfoI 53 GCGC GCG^C
612 GCGC
DdeI 35 CTGAG C^T[ACGT]AG
481 CTGAG
HhaI 53 GCGC GCG^C
612 GCGC
HinP1I 53 GCGC G^CGC
612 GCGC
HphI 205 TCACC GGTGA(8/7)
245 TCACC
MslI 270 CATCAACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
292 CATCAGCATG
PleI 254 GAGTC GAGTC(4/5)
483 GAGTC
SphI 421 GCATGC GCATG^C
705 GCATGC
TaqI 44 TCGA T^CGA
369 TCGA
Tsp45I 403 GTGAC ^GT[GC]AC
670 GTCAC
TthHB8I 44 TCGA T^CGA
369 TCGA
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# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AciI BanII BbvI BcnI BmyI
BsaJI BsiYI BslI Bsp1286I BssKI
BstF5I BstNI Cac8I Cfr13I DraII
EcoO109I EcoRII FauI Fnu4HI FokI
HaeIII HapII HinfI HpaII ItaI
MaeII MaeIII MnlI MspI MvaI
MwoI NciI NlaIII NlaIV NspI
Sau96I ScrFI SfaNI TseI TspRI