Restriction map for
pFN21AE0207
(Linear) (Six-base) MAPSORT of: KIEE0207.seq Check: 4706 from: 1 to: 831
KIEE0207.orf
With 182 enzymes: SgfI *
April 24, 2009 13:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 256 831
Size: 256 575
AflIII A'CryG_T
Cuts at: 0 622 831
Size: 622 209
AlwNI CAG_nnn'CTG
Cuts at: 0 673 831
Size: 673 158
ApaI G_GGCC'C
Cuts at: 0 80 101 831
Size: 80 21 730
Fragments arranged by size:
730 80 21
AvaI C'yCGr_G
Cuts at: 0 100 157 831
Size: 100 57 674
Fragments arranged by size:
674 100 57
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 609 642 831
Size: 609 33 189
Fragments arranged by size:
609 189 33
BanII G_rGCy'C
Cuts at: 0 41 80 101 783 831
Size: 41 39 21 682 48
Fragments arranged by size:
682 48 41 39 21
BclI T'GATC_A
Cuts at: 0 242 831
Size: 242 589
BfrBI ATG'CAT
Cuts at: 0 291 831
Size: 291 540
Bme1580I G_kGCm'C
Cuts at: 0 80 101 237 831
Size: 80 21 136 594
Fragments arranged by size:
594 136 80 21
BsaAI yAC'GTr
Cuts at: 0 625 831
Size: 625 206
BseYI C'CCAG_C
Cuts at: 0 94 831
Size: 94 737
BsiWI C'GTAC_G
Cuts at: 0 131 831
Size: 131 700
BsmI GAATG_Cn'
Cuts at: 0 348 831
Size: 348 483
Bsp1286I G_dGCh'C
Cuts at: 0 41 80 101 237 783 831
Size: 41 39 21 136 546 48
Fragments arranged by size:
546 136 48 41 39 21
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 256 831
Size: 256 575
BsrDI GCAATG_nn'
Cuts at: 0 327 525 831
Size: 327 198 306
Fragments arranged by size:
327 306 198
BstAPI GCAn_nnn'nTGC
Cuts at: 0 673 831
Size: 673 158
BtgI C'CryG_G
Cuts at: 0 500 831
Size: 500 331
BtsI GCAGTG_nn'
Cuts at: 0 585 671 831
Size: 585 86 160
Fragments arranged by size:
585 160 86
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 666 701 831
Size: 666 35 130
Fragments arranged by size:
666 130 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 482 831
Size: 482 349
EcoO109I rG'GnC_Cy
Cuts at: 0 76 189 460 831
Size: 76 113 271 371
Fragments arranged by size:
371 271 113 76
MslI CAynn'nnrTG
Cuts at: 0 274 296 831
Size: 274 22 535
Fragments arranged by size:
535 274 22
MspA1I CmG'CkG
Cuts at: 0 27 831
Size: 27 804
NcoI C'CATG_G
Cuts at: 0 500 831
Size: 500 331
NsiI A_TGCA'T
Cuts at: 0 293 831
Size: 293 538
NspI r_CATG'y
Cuts at: 0 301 425 709 831
Size: 301 124 284 122
Fragments arranged by size:
301 284 124 122
PasI CC'CwG_GG
Cuts at: 0 352 831
Size: 352 479
PflMI CCAn_nnn'nTGG
Cuts at: 0 501 831
Size: 501 330
PfoI T'CCnGG_A
Cuts at: 0 471 831
Size: 471 360
PpuMI rG'GwC_Cy
Cuts at: 0 189 831
Size: 189 642
PspOMI G'GGCC_C
Cuts at: 0 76 97 831
Size: 76 21 734
Fragments arranged by size:
734 76 21
PstI C_TGCA'G
Cuts at: 0 592 831
Size: 592 239
SfcI C'TryA_G
Cuts at: 0 588 831
Size: 588 243
SmaI CCC'GGG
Cuts at: 0 102 831
Size: 102 729
SphI G_CATG'C
Cuts at: 0 425 709 831
Size: 425 284 122
Fragments arranged by size:
425 284 122
StuI AGG'CCT
Cuts at: 0 110 831
Size: 110 721
StyI C'CwwG_G
Cuts at: 0 500 831
Size: 500 331
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 493 831
Size: 493 338
XmaI C'CCGG_G
Cuts at: 0 100 831
Size: 100 731
Enzymes that do cut:
AarI AflIII AlwNI ApaI AvaI BaeI BanII BclI
BfrBI Bme1580I BsaAI BseYI BsiWI BsmI Bsp1286I BspMI
BsrDI BstAPI BtgI BtsI CspCI DrdI EcoO109I MslI
MspA1I NcoI NsiI NspI PasI PflMI PfoI PpuMI
PspOMI PstI SfcI SmaI SphI StuI StyI TaqII
XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AgeI
AhdI AleI AlfI AloI ApaLI ApoI AscI AseI
AsiSI AvrII BamHI BanI BbeI BbsI BbvCI BcgI
BciVI BglI BglII BlpI BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI BsaXI
BseRI BsgI BsiEI BsiHKAI BsmBI BspEI BspHI BsrBI
BsrFI BsrGI BssHII BssSI BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtgZI ClaI DraI DraIII EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoRI EcoRV FalI
FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI NaeI NarI
NdeI NgoMIV NheI NotI NruI PacI PciI PmeI
PmlI PpiI PshAI PsiI PspXI PsrI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI
SrfI SspI SwaI TatI TstI Tth111I XbaI XcmI
XhoI XmnI ZraI