Restriction map for
pFN21AE0522
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# NO CUT ENZYMES ( 192 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaI
AviII AvrII BalI BamHI BanI
BanII BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BcnI BfrI
BglI BglII BlnI BlpI BmyI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BseRI BsgI
BsiEI BsiHKAI BsiWI BsiYI BslI
BsmBI BsmFI BsmI BsoBI Bsp1286I
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DraIII DrdI
EaeI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FauI FbaI
FseI FspI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HinfI HpaI HpaII
KasI KpnI Ksp632I KspI MaeII
MamI MfeI MluI MluNI MroI
MscI MslI MspA1I MspI MunI
MvnI NaeI NarI NciI NdeI
NgoMIV NlaIV NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TfiI Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AciI 184 GCGG CCGC(-3/-1)
AcsI 411 AAATTC [GA]^AATT[CT]
AfaI 243 GTAC GT^AC
AlwNI 263 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 411 AAATTC [GA]^AATT[CT]
AvaII 265 GGACC G^G[AT]CC
BpmI 246 CTCCAG CTGGAG(16/14)
BsmAI 313 GTCTC GTCTC(1/5)
BspMI 267 ACCTGC ACCTGC(4/8)
BsrDI 323 GCAATG GCAATG(2/0)
BsrI 136 ACTGG ACTGG(1/-1)
BstAPI 262 GCAGGACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 116 CATCC GGATG(2/0)
BstYI 360 GGATCT [GA]^GATC[CT]
Cac8I 222 GCTAGC GC[ACGT]^[ACGT]GC
Cfr13I 265 GGACC G^G[ACGT]CC
DraI 157 TTTAAA TTT^AAA
DraII 264 AGGACCT [GA]G^G[ACGT]CC[CT]
DsaI 62 CCATGG C^C[GA][CT]GG
EcoO109I 264 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoT14I 62 CCATGG C^C[AT][AT]GG
FokI 116 CATCC GGATG(9/13)
MflI 360 GGATCT [GA]^GATC[CT]
MwoI 262 GCAGGACCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 62 CCATGG C^CATGG
NheI 222 GCTAGC G^CTAGC
PpuMI 264 AGGACCT [GA]G^G[AT]CC[CT]
PstI 217 CTGCAG CTGCA^G
RsaI 243 GTAC GT^AC
Sau96I 265 GGACC G^G[ACGT]CC
SfaNI 115 GCATC GCATC(5/9)
SmlI 196 CTTGAG C^T[CT][GA]AG
StyI 62 CCATGG C^C[AT][AT]GG
TaqI 229 TCGA T^CGA
Tsp45I 256 GTGAC ^GT[GC]AC
TthHB8I 229 TCGA T^CGA
XhoII 360 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AlwI 190 GGATC GGATC(4/5)
360 GGATC
BbvI 219 GCAGC GCAGC(8/12)
216 GCTGC
DdeI 179 CTAAG C^T[ACGT]AG
382 CTTAG
DpnII 191 GATC ^GATC
361 GATC
Fnu4HI 216 GCTGC GC^[ACGT]GC
219 GCAGC
HphI 255 GGTGA GGTGA(8/7)
316 TCACC
ItaI 216 GCTGC GC^[ACGT]GC
219 GCAGC
MboI 191 GATC ^GATC
361 GATC
MboII 339 TCTTC GAAGA(8/7)
342 TCTTC
MnlI 272 CCTC CCTC(7/6)
175 GAGG
NdeII 191 GATC ^GATC
361 GATC
NlaIII 63 CATG CATG^
370 CATG
Sau3AI 191 GATC ^GATC
361 GATC
SfcI 217 CTGCAG C^T[GA][CT]AG
308 CTACAG
TseI 216 GCTGC G^C[AT]GC
219 GCAGC
TspRI 12 CACTG CA[GC]TG[ACGT][ACGT]^
239 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 13 enzymes) #
#########################################
AluI BfaI BsaJI BssKI BstNI
EcoRII MaeI MaeIII MseI MvaI
ScrFI Tru9I Tsp509I