(Circular) (Six-base) MAPSORT of: pFN21AE0490.seq Check: 6174 from: 1 to: 5926 pFN21AE0490 5926 bp With 182 enzymes: SgfI * October 20, 2009 10:48 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2217 2483 3088 2217 Size: 266 605 5055 Fragments arranged by size: 5055 605 266 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2625 3736 278 Size: 53 83 186 936 1089 1111 2468 Fragments arranged by size: 2468 1111 1089 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3269 1937 Size: 1332 4594 Acc65I G'GTAC_C Cuts at: 3247 3247 Size: 5926 AclI AA'CG_TT Cuts at: 1563 4053 4426 1563 Size: 2490 373 3063 Fragments arranged by size: 3063 2490 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3989 5373 5641 1632 Size: 2357 1384 268 1917 Fragments arranged by size: 2357 1917 1384 268 AfeI AGC'GCT Cuts at: 2240 2240 Size: 5926 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5729 AflIII A'CryG_T Cuts at: 1203 2328 4825 5802 1203 Size: 1125 2497 977 1327 Fragments arranged by size: 2497 1327 1125 977 AgeI A'CCGG_T Cuts at: 4757 4757 Size: 5926 AhdI GACnn_n'nnGTC Cuts at: 4655 4655 Size: 5926 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5926 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5926 AlwNI CAG_nnn'CTG Cuts at: 3669 5241 3669 Size: 1572 4354 ApaI G_GGCC'C Cuts at: 3126 3126 Size: 5926 ApaLI G'TGCA_C Cuts at: 3983 5139 3983 Size: 1156 4770 ApoI r'AATT_y Cuts at: 1493 3221 3235 3461 3613 5722 1493 Size: 1728 14 226 152 2109 1697 Fragments arranged by size: 2109 1728 1697 226 152 14 AseI AT'TA_AT Cuts at: 160 1032 4480 160 Size: 872 3448 1606 Fragments arranged by size: 3448 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5926 AvaI C'yCGr_G Cuts at: 1870 1943 2087 2712 3251 1870 Size: 73 144 625 539 4545 Fragments arranged by size: 4545 625 539 144 73 AvrII C'CTAG_G Cuts at: 2404 2404 Size: 5926 BamHI G'GATC_C Cuts at: 2035 3256 3623 2035 Size: 1221 367 4338 Fragments arranged by size: 4338 1221 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2339 2471 2867 3140 3176 Size: 325 217 627 552 132 396 273 36 Cuts at: 3176 3247 4702 618 Size: 71 1455 1842 Fragments arranged by size: 1842 1455 627 552 396 325 273 217 132 71 36 BanII G_rGCy'C Cuts at: 1391 3126 3245 1391 Size: 1735 119 4072 Fragments arranged by size: 4072 1735 119 BbeI G_GCGC'C Cuts at: 3144 3180 3144 Size: 36 5890 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3200 928 Size: 971 1301 3654 Fragments arranged by size: 3654 1301 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2046 2080 4117 4151 1903 Size: 34 109 34 2037 34 3678 Fragments arranged by size: 3678 2037 109 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3818 5028 3818 Size: 1210 4716 BclI T'GATC_A Cuts at: 1548 1548 Size: 5926 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2144 3130 3174 Size: 107 122 71 631 605 472 986 44 Cuts at: 3174 4537 136 Size: 1363 1525 Fragments arranged by size: 1525 1363 986 631 605 472 122 107 71 44 BglII A'GATC_T Cuts at: 2196 5921 2196 Size: 3725 2201 BlpI GC'TnA_GC Cuts at: 2294 2723 3206 3343 2294 Size: 429 483 137 4877 Fragments arranged by size: 4877 483 429 137 Bme1580I G_kGCm'C Cuts at: 1790 2271 2870 2998 3126 3987 5143 1790 Size: 481 599 128 128 861 1156 2573 Fragments arranged by size: 2573 1156 861 599 481 128 128 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5926 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3657 4615 442 Size: 416 53 83 399 24 2240 958 1753 Fragments arranged by size: 2240 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5926 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2570 2831 2998 3134 4586 1239 Size: 1331 261 167 136 1452 2579 Fragments arranged by size: 2579 1452 1331 261 167 136 Bpu10I CC'TnA_GC Cuts at: 2142 2384 2142 Size: 242 5684 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2729 3008 4051 4916 5214 2729 Size: 279 1043 865 298 3441 Fragments arranged by size: 3441 1043 865 298 279 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3023 4589 882 Size: 617 1524 1566 2219 Fragments arranged by size: 2219 1566 1524 617 BsaAI yAC'GTr Cuts at: 493 1204 2329 2644 3717 493 Size: 711 1125 315 1073 2702 Fragments arranged by size: 2702 1125 1073 711 315 BsaBI GATnn'nnATC Cuts at: 5920 5920 Size: 5926 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2622 3060 3141 3177 Size: 53 83 186 936 1089 438 81 36 Cuts at: 3177 3733 4115 4750 275 Size: 556 382 635 1451 Fragments arranged by size: 1451 1089 936 635 556 438 382 186 83 81 53 36 BsaWI w'CCGG_w Cuts at: 4359 4757 5031 5178 5589 4359 Size: 398 274 147 411 4696 Fragments arranged by size: 4696 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2648 2678 3137 3167 1178 Size: 30 165 30 1245 30 459 30 3937 Fragments arranged by size: 3937 1245 459 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2291 2424 1186 Size: 1105 133 4688 Fragments arranged by size: 4688 1105 133 BseYI C'CCAG_C Cuts at: 2069 5129 5625 2069 Size: 3060 496 2370 Fragments arranged by size: 3060 2370 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3074 1163 Size: 573 1338 4015 Fragments arranged by size: 4015 1338 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2055 3376 4137 5165 1540 Size: 275 186 54 1321 761 1028 2301 Fragments arranged by size: 2301 1321 1028 761 275 186 54 BsiHKAI G_wGCw'C Cuts at: 3987 4072 5143 3987 Size: 85 1071 4770 Fragments arranged by size: 4770 1071 85 BsmI GAATG_Cn' Cuts at: 3437 3530 3437 Size: 93 5833 BsmBI CGTCTCn'nnnn_ Cuts at: 2080 2080 Size: 5926 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2271 2870 2998 3126 3245 3987 4072 Size: 399 481 599 128 128 119 742 85 Cuts at: 4072 5143 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 599 481 399 128 128 119 85 BspEI T'CCGG_A Cuts at: 5589 5589 Size: 5926 BspHI T'CATG_A Cuts at: 3815 5545 3815 Size: 1730 4196 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2217 2483 3088 3281 844 Size: 1373 266 605 193 3489 Fragments arranged by size: 3489 1373 605 266 193 BsrBI CCG'CTC Cuts at: 1394 1823 2141 3159 3813 1394 Size: 429 318 1018 654 3507 Fragments arranged by size: 3507 1018 654 429 318 BsrDI GCAATG_nn' Cuts at: 65 1248 4421 4595 65 Size: 1183 3173 174 1396 Fragments arranged by size: 3173 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2879 4570 4757 1700 Size: 110 1069 1691 187 2869 Fragments arranged by size: 2869 1691 1069 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5926 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5926 BssSI C'ACGA_G Cuts at: 3986 4998 3986 Size: 1012 4914 BstAPI GCAn_nnn'nTGC Cuts at: 794 2478 2794 794 Size: 1684 316 3926 Fragments arranged by size: 3926 1684 316 BstBI TT'CG_AA Cuts at: 4730 4730 Size: 5926 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5926 BstXI CCAn_nnnn'nTGG Cuts at: 2107 2107 Size: 5926 BstYI r'GATC_y Cuts at: 1972 2035 2196 3256 3623 4008 4025 5477 5563 Size: 63 161 1060 367 385 17 1452 86 Cuts at: 5563 5575 5921 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1060 385 367 346 161 86 63 17 12 BtgI C'CryG_G Cuts at: 513 1065 2106 2871 3145 513 Size: 552 1041 765 274 3294 Fragments arranged by size: 3294 1041 765 552 274 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5187 Fragments arranged by size: 5187 683 56 BtsI GCAGTG_nn' Cuts at: 2100 3442 4236 4256 2100 Size: 1342 794 20 3770 Fragments arranged by size: 3770 1342 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5926 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5891 DraI TTT'AAA Cuts at: 3230 3230 Size: 5926 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2621 4933 809 Size: 507 1305 2312 1802 Fragments arranged by size: 2312 1802 1305 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2802 3373 4262 Size: 54 1425 50 275 18 972 571 889 Cuts at: 4262 5891 8 Size: 1629 43 Fragments arranged by size: 1629 1425 972 889 571 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3373 1537 Size: 275 1561 4090 Fragments arranged by size: 4090 1561 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2264 2309 2696 2702 2999 3856 1353 Size: 911 45 387 6 297 857 3423 Fragments arranged by size: 3423 911 857 387 297 45 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4513 4887 5033 387 Size: 1064 138 2924 374 146 1280 Fragments arranged by size: 2924 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2570 2831 2998 3134 3989 4586 5373 Size: 393 938 261 167 136 855 597 787 Cuts at: 5373 5641 1239 Size: 268 1524 Fragments arranged by size: 1524 938 855 787 597 393 268 261 167 136 EcoRI G'AATT_C Cuts at: 3221 3235 3613 3221 Size: 14 378 5534 Fragments arranged by size: 5534 378 14 FspI TGC'GCA Cuts at: 2252 4432 2252 Size: 2180 3746 FspAI rTGC'GCAy Cuts at: 2252 2252 Size: 5926 HaeII r_GCGC'y Cuts at: 1678 2242 3144 3180 5073 1678 Size: 564 902 36 1893 2531 Fragments arranged by size: 2531 1893 902 564 36 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2027 2059 4567 4599 4641 Size: 32 135 32 455 32 2508 32 42 Cuts at: 4641 4673 1373 Size: 32 2626 Fragments arranged by size: 2626 2508 455 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3270 3516 669 Size: 988 281 1332 246 3079 Fragments arranged by size: 3079 1332 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5926 HpaI GTT'AAC Cuts at: 3516 3516 Size: 5926 KasI G'GCGC_C Cuts at: 3140 3176 3140 Size: 36 5890 KpnI G_GTAC'C Cuts at: 3251 3251 Size: 5926 MfeI C'AATT_G Cuts at: 3525 3525 Size: 5926 MluI A'CGCG_T Cuts at: 5802 5802 Size: 5926 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2409 5040 5224 1305 Size: 1104 2631 184 2007 Fragments arranged by size: 2631 2007 1104 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2804 10 Size: 54 1425 343 972 3132 Fragments arranged by size: 3132 1425 972 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2105 2903 3884 4243 4402 518 Size: 648 843 96 798 981 359 159 2042 Fragments arranged by size: 2042 981 843 798 648 359 159 96 MspA1I CmG'CkG Cuts at: 1606 2223 2460 2991 3342 4019 5167 5412 1606 Size: 617 237 531 351 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 617 531 351 245 237 NaeI GCC'GGC Cuts at: 2881 2881 Size: 5926 NarI GG'CG_CC Cuts at: 3141 3177 3141 Size: 36 5890 NcoI C'CATG_G Cuts at: 513 1065 2106 513 Size: 552 1041 4333 Fragments arranged by size: 4333 1041 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5926 NgoMIV G'CCGG_C Cuts at: 2879 2879 Size: 5926 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5926 NotI GC'GGCC_GC Cuts at: 3373 3373 Size: 5926 NspI r_CATG'y Cuts at: 1132 1229 2190 2206 2797 3284 4829 1132 Size: 97 961 16 591 487 1545 2229 Fragments arranged by size: 2229 1545 961 591 487 97 16 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5926 PciI A'CATG_T Cuts at: 4825 4825 Size: 5926 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5926 PfoI T'CCnGG_A Cuts at: 2808 2808 Size: 5926 PmeI GTTT'AAAC Cuts at: 3230 3230 Size: 5926 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3995 4027 5508 5540 3995 Size: 32 1481 32 4381 Fragments arranged by size: 4381 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5926 PsiI TTA'TAA Cuts at: 3496 3496 Size: 5926 PspOMI G'GGCC_C Cuts at: 3122 3122 Size: 5926 PstI C_TGCA'G Cuts at: 830 2541 3278 830 Size: 1711 737 3478 Fragments arranged by size: 3478 1711 737 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5926 PvuII CAG'CTG Cuts at: 2223 2460 2223 Size: 237 5689 SalI G'TCGA_C Cuts at: 1936 3268 1936 Size: 1332 4594 SapI GCTCTTCn'nnn_ Cuts at: 2999 2999 Size: 5926 SbfI CC_TGCA'GG Cuts at: 3278 3278 Size: 5926 ScaI AGT'ACT Cuts at: 2324 4174 5886 2324 Size: 1850 1712 2364 Fragments arranged by size: 2364 1850 1712 SfcI C'TryA_G Cuts at: 826 1045 2218 2537 2731 3274 4409 5090 5281 Size: 219 1173 319 194 543 1135 681 191 Cuts at: 5281 826 Size: 1471 Fragments arranged by size: 1471 1173 1135 681 543 319 219 194 191 SfoI GGC'GCC Cuts at: 3142 3178 3142 Size: 36 5890 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5926 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5926 SmaI CCC'GGG Cuts at: 1872 2714 3253 1872 Size: 842 539 4545 Fragments arranged by size: 4545 842 539 SmlI C'TyrA_G Cuts at: 820 1017 1943 2744 3023 4030 4931 5193 820 Size: 197 926 801 279 1007 901 262 1553 Fragments arranged by size: 1553 1007 926 901 801 279 262 197 SnaBI TAC'GTA Cuts at: 493 3717 493 Size: 3224 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5926 SphI G_CATG'C Cuts at: 1132 2797 3284 1132 Size: 1665 487 3774 Fragments arranged by size: 3774 1665 487 SspI AAT'ATT Cuts at: 5 52 3850 5 Size: 47 3798 2081 Fragments arranged by size: 3798 2081 47 StuI AGG'CCT Cuts at: 1517 2363 2591 1517 Size: 846 228 4852 Fragments arranged by size: 4852 846 228 StyI C'CwwG_G Cuts at: 513 1065 2106 2364 2404 3365 513 Size: 552 1041 258 40 961 3074 Fragments arranged by size: 3074 1041 961 552 258 40 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3953 3970 4123 4308 4753 325 Size: 1255 2373 17 153 185 445 1498 Fragments arranged by size: 2373 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2322 2331 3093 Size: 274 80 33 51 1443 345 9 762 Cuts at: 3093 4172 5884 96 Size: 1079 1712 138 Fragments arranged by size: 1712 1443 1079 762 345 274 138 80 51 33 9 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3137 3169 5886 5918 3137 Size: 32 2717 32 3145 Fragments arranged by size: 3145 2717 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5926 XbaI T'CTAG_A Cuts at: 3262 3262 Size: 5926 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3170 3170 Size: 5926 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5926 XmaI C'CCGG_G Cuts at: 1870 2712 3251 1870 Size: 842 539 4545 Fragments arranged by size: 4545 842 539 XmnI GAAnn'nnTTC Cuts at: 4055 4055 Size: 5926 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2623 3734 276 Size: 53 83 186 936 1089 1111 2468 Fragments arranged by size: 2468 1111 1089 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI BaeI BbvCI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SrfI SwaI