Restriction map for
pFN21AE0418
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# NO CUT ENZYMES ( 120 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaLI AscI AseI
AsnI Asp718I AspEI AspHI AspI
AviII AvrII BanI BbeI BbvCI
BciVI BclI BfaI BfrI BglII
BlnI BlpI Bpu1102I BsaBI BsaWI
BseAI BsgI BsiHKAI BsiWI BspDI
BspEI BspHI BspLU11I BspMI BsrDI
BssHII Bst1107I BstAPI BstBI BstZ17I
Bsu36I CelII ClaI CpoI DraI
Eam1105I Eco47III Eco81I EcoNI EcoRV
EcoT22I FbaI FseI FspI HaeII
HincII HindII HindIII HpaI KasI
KpnI KspI MaeI MamI MfeI
MluI MroI MseI MunI NarI
NcoI NdeI NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmeI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspI
SwaI Tru9I Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccII 20 CGCG CG^CG
AcsI 304 GAATTC [GA]^AATT[CT]
AlwNI 1143 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 304 GAATTC [GA]^AATT[CT]
BamHI 221 GGATCC G^GATCC
BbrPI 292 CACGTG CAC^GTG
BglI 661 GCCACGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 292 CACGTG [CT]AC^GT[GA]
BsiEI 27 CGGCCG CG[GA][CT]^CG
BsmBI 826 CGTCTC CGTCTC(1/5)
BsmI 1089 GAATGC GAATGC(1/-1)
Bsp1407I 1073 TGTACA T^GTACA
BsrBI 131 GAGCGG CCGCTC(-3/-3)
BsrFI 1014 GCCGGC [GA]^CCGG[CT]
BsrGI 1073 TGTACA T^GTACA
BstEII 138 GGTGACC G^GT[ACGT]ACC
BstPI 138 GGTGACC G^GT[ACGT]ACC
BstUI 20 CGCG CG^CG
BstXI 152 CCAGCCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 21 GCGC GCG^C
Cfr10I 1014 GCCGGC [GA]^CCGG[CT]
DraIII 868 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 27 CGGCCG C^GGCCG
EarI 643 GAAGAG CTCTTC(1/4)
EclXI 27 CGGCCG C^GGCCG
Eco52I 27 CGGCCG C^GGCCG
EcoO65I 138 GGTGACC G^GT[ACGT]ACC
EcoRI 304 GAATTC G^AATTC
EcoT14I 164 CCAAGG C^C[AT][AT]GG
HhaI 21 GCGC GCG^C
HinP1I 21 GCGC G^CGC
Ksp632I 643 GAAGAG CTCTTC(1/4)
MaeII 293 ACGT A^CGT
MvnI 20 CGCG CG^CG
NaeI 1014 GCCGGC GCC^GGC
NgoMIV 1014 GCCGGC G^CCGGC
PmaCI 292 CACGTG CAC^GTG
PmlI 292 CACGTG CAC^GTG
PstI 712 CTGCAG CTGCA^G
PvuII 531 CAGCTG CAG^CTG
SmaI 298 CCCGGG CCC^GGG
SspBI 1073 TGTACA T^GTACA
StuI 84 AGGCCT AGG^CCT
StyI 164 CCAAGG C^C[AT][AT]GG
TaqI 726 TCGA T^CGA
TfiI 1007 GAATC G^A[AT]TC
TthHB8I 726 TCGA T^CGA
XmaCI 298 CCCGGG C^CCGGG
XmaI 298 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcyI 196 GACGCC G[GA]^CG[CT]C
824 GGCGTC
ApaI 62 GGGCCC GGGCC^C
745 GGGCCC
Asp700I 718 GAAACCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1204 GAAATTGTTC
AvaI 298 CCCGGG C^[CT]CG[GA]G
495 CCCGAG
BbsI 568 GAAGAC GAAGAC(2/6)
681 GAAGAC
BcgI 111 CGATAGCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
10 GCAGTGTGGTCG
BpuAI 568 GAAGAC GAAGAC(2/6)
681 GAAGAC
BsaHI 196 GACGCC G[GA]^CG[CT]C
824 GGCGTC
BsaI 262 GGTCTC GGTCTC(1/5)
312 GGTCTC
BseRI 484 CTCCTC GAGGAG(10/8)
806 CTCCTC
BsoBI 298 CCCGGG C^[CT]CG[GA]G
495 CCCGAG
BsrI 218 ACTGG ACTGG(1/-1)
244 ACTGG
BssSI 663 CACGAG CACGAG(-5/-1)
397 CTCGTG
BstYI 221 GGATCC [GA]^GATC[CT]
986 AGATCC
DpnII 222 GATC ^GATC
987 GATC
DrdI 406 GACCTTGTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
571 GACTCCGTGGTC
HgaI 196 GACGC GACGC(5/10)
825 GCGTC
Hin1I 196 GACGCC G[GA]^CG[CT]C
824 GGCGTC
MboI 222 GATC ^GATC
987 GATC
MflI 221 GGATCC [GA]^GATC[CT]
986 AGATCC
NdeII 222 GATC ^GATC
987 GATC
PleI 571 GACTC GAGTC(4/5)
1185 GACTC
Sau3AI 222 GATC ^GATC
987 GATC
SfcI 528 CTACAG C^T[GA][CT]AG
712 CTGCAG
SmlI 508 CTCAAG C^T[CT][GA]AG
829 CTCAAG
XhoII 221 GGATCC [GA]^GATC[CT]
986 AGATCC
XmnI 718 GAAACCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1204 GAAATTGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BalI BanII BbvI BcnI BmyI
BpmI BsaJI BsiYI BslI BsmAI
BsmFI Bsp1286I BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DraII DsaI
EaeI EcoO109I EcoRII FauI Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI MaeIII MboII MluNI
MnlI MscI MslI MspA1I MspI
MvaI MwoI NciI NlaIII NlaIV
PflMI PpuMI RsaI Sau96I ScrFI
SfaNI TseI Tsp45I Tsp509I TspRI
Van91I XcmI