(Circular) (Six-base) MAPSORT of: pFN21AE0418.seq Check: 1014 from: 1 to: 5974 pFN21AE0418 5974 bp With 182 enzymes: SgfI * October 20, 2009 10:47 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3784 278 Size: 53 83 186 936 2248 2468 Fragments arranged by size: 2468 2248 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3317 1937 Size: 1380 4594 Acc65I G'GTAC_C Cuts at: 3295 3295 Size: 5974 AclI AA'CG_TT Cuts at: 1563 4101 4474 1563 Size: 2538 373 3063 Fragments arranged by size: 3063 2538 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2592 2732 2902 2926 4037 5421 5689 1632 Size: 960 140 170 24 1111 1384 268 1917 Fragments arranged by size: 1917 1384 1111 960 268 170 140 24 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5777 AflIII A'CryG_T Cuts at: 1203 4873 5850 1203 Size: 3670 977 1327 Fragments arranged by size: 3670 1327 977 AgeI A'CCGG_T Cuts at: 4805 4805 Size: 5974 AhdI GACnn_n'nnGTC Cuts at: 4703 4703 Size: 5974 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5974 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5974 AlwNI CAG_nnn'CTG Cuts at: 3153 3717 5289 3153 Size: 564 1572 3838 Fragments arranged by size: 3838 1572 564 ApaI G_GGCC'C Cuts at: 2071 2754 2071 Size: 683 5291 ApaLI G'TGCA_C Cuts at: 4031 5187 4031 Size: 1156 4818 ApoI r'AATT_y Cuts at: 1493 2309 3283 3509 3661 5770 1493 Size: 816 974 226 152 2109 1697 Fragments arranged by size: 2109 1697 974 816 226 152 AseI AT'TA_AT Cuts at: 160 1032 4528 160 Size: 872 3496 1606 Fragments arranged by size: 3496 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5974 AvaI C'yCGr_G Cuts at: 1870 1943 2303 2500 3299 1870 Size: 73 360 197 799 4545 Fragments arranged by size: 4545 799 360 197 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2966 2999 2966 Size: 33 5941 BamHI G'GATC_C Cuts at: 2226 3304 3671 2226 Size: 1078 367 4529 Fragments arranged by size: 4529 1078 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3295 4750 618 Size: 325 217 627 1508 1455 1842 Fragments arranged by size: 1842 1508 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2071 2754 2810 3293 1391 Size: 680 683 56 483 4072 Fragments arranged by size: 4072 683 680 483 56 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2580 2693 928 Size: 971 681 113 4209 Fragments arranged by size: 4209 971 681 113 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2004 2038 2105 2139 4165 4199 1903 Size: 34 67 34 67 34 2026 34 3678 Fragments arranged by size: 3678 2026 67 67 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3866 5076 3866 Size: 1210 4764 BclI T'GATC_A Cuts at: 1548 1548 Size: 5974 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2672 4585 136 Size: 107 122 71 631 605 1000 1913 1525 Fragments arranged by size: 1913 1525 1000 631 605 122 107 71 BglII A'GATC_T Cuts at: 5969 5969 Size: 5974 BlpI GC'TnA_GC Cuts at: 3391 3391 Size: 5974 Bme1580I G_kGCm'C Cuts at: 1790 2071 2304 2754 4035 5191 1790 Size: 281 233 450 1281 1156 2573 Fragments arranged by size: 2573 1281 1156 450 281 233 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5974 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3705 4663 442 Size: 416 53 83 399 24 2288 958 1753 Fragments arranged by size: 2288 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5974 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2829 3154 2829 Size: 325 5649 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2876 2992 3206 4634 1239 Size: 1637 116 214 1428 2579 Fragments arranged by size: 2579 1637 1428 214 116 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2498 2819 4099 4964 5262 2498 Size: 321 1280 865 298 3210 Fragments arranged by size: 3210 1280 865 321 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2273 2323 4637 882 Size: 617 774 50 2314 2219 Fragments arranged by size: 2314 2219 774 617 50 BsaAI yAC'GTr Cuts at: 493 1204 2299 3765 493 Size: 711 1095 1466 2702 Fragments arranged by size: 2702 1466 1095 711 BsaBI GATnn'nnATC Cuts at: 5968 5968 Size: 5974 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2202 2830 3781 4163 Size: 53 83 186 936 669 628 951 382 Cuts at: 4163 4798 275 Size: 635 1451 Fragments arranged by size: 1451 951 936 669 635 628 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4407 4805 5079 5226 5637 4407 Size: 398 274 147 411 4744 Fragments arranged by size: 4744 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2127 2157 1178 Size: 30 165 30 724 30 4995 Fragments arranged by size: 4995 724 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2480 2802 1186 Size: 1294 322 4358 Fragments arranged by size: 4358 1294 322 BseYI C'CCAG_C Cuts at: 2447 2538 5177 5673 2447 Size: 91 2639 496 2748 Fragments arranged by size: 2748 2639 496 91 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5401 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2035 3424 4185 5213 1540 Size: 275 186 34 1389 761 1028 2301 Fragments arranged by size: 2301 1389 1028 761 275 186 34 BsiHKAI G_wGCw'C Cuts at: 4035 4120 5191 4035 Size: 85 1071 4818 Fragments arranged by size: 4818 1071 85 BsmI GAATG_Cn' Cuts at: 3100 3485 3578 3100 Size: 385 93 5496 Fragments arranged by size: 5496 385 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2837 2837 Size: 5974 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2071 2304 2754 2810 3293 4035 4120 Size: 399 281 233 450 56 483 742 85 Cuts at: 4120 5191 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 483 450 399 281 233 85 56 BspEI T'CCGG_A Cuts at: 5637 5637 Size: 5974 BspHI T'CATG_A Cuts at: 3863 5593 3863 Size: 1730 4244 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3329 844 Size: 2485 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2138 3861 1394 Size: 429 315 1723 3507 Fragments arranged by size: 3507 1723 429 315 BsrDI GCAATG_nn' Cuts at: 65 1248 4469 4643 65 Size: 1183 3221 174 1396 Fragments arranged by size: 3221 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3019 4618 4805 1700 Size: 110 1209 1599 187 2869 Fragments arranged by size: 2869 1599 1209 187 110 BsrGI T'GTAC_A Cuts at: 96 3078 96 Size: 2982 2992 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5974 BssSI C'ACGA_G Cuts at: 2402 2668 4034 5046 2402 Size: 266 1366 1012 3330 Fragments arranged by size: 3330 1366 1012 266 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5974 BstBI TT'CG_AA Cuts at: 4778 4778 Size: 5974 BstEII G'GTnAC_C Cuts at: 1184 2143 1184 Size: 959 5015 BstXI CCAn_nnnn'nTGG Cuts at: 2164 2164 Size: 5974 BstYI r'GATC_y Cuts at: 1972 2226 2991 3304 3671 4056 4073 5525 5611 Size: 254 765 313 367 385 17 1452 86 Cuts at: 5611 5623 5969 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 765 385 367 346 313 254 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2130 2580 2883 3226 3270 513 Size: 552 939 126 450 303 343 44 3217 Fragments arranged by size: 3217 939 552 450 343 303 126 44 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5235 Fragments arranged by size: 5235 683 56 BtsI GCAGTG_nn' Cuts at: 2022 2331 3490 4284 4304 2022 Size: 309 1159 794 20 3692 Fragments arranged by size: 3692 1159 794 309 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5974 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5939 DraI TTT'AAA Cuts at: 3278 3278 Size: 5974 DraIII CAC_nnn'GTG Cuts at: 2878 2878 Size: 5974 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2417 2582 4981 809 Size: 507 1101 165 2399 1802 Fragments arranged by size: 2399 1802 1101 507 165 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2032 2082 2154 Size: 54 1425 50 275 18 202 50 72 Cuts at: 2154 2166 2293 2364 2886 3421 4310 5939 8 Size: 12 127 71 522 535 889 1629 43 Fragments arranged by size: 1629 1425 889 535 522 275 202 127 72 71 54 50 50 43 18 12 EagI C'GGCC_G Cuts at: 1537 1812 2032 3421 1537 Size: 275 220 1389 4090 Fragments arranged by size: 4090 1389 275 220 EarI CTCTTCn'nnn_ Cuts at: 1353 2643 3904 1353 Size: 1290 1261 3423 Fragments arranged by size: 3423 1290 1261 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2654 2692 4561 4935 5081 387 Size: 1064 138 1065 38 1869 374 146 1280 Fragments arranged by size: 1869 1280 1065 1064 374 146 138 38 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2592 2732 2876 2902 2926 2992 3206 Size: 393 960 140 144 26 24 66 214 Cuts at: 3206 4037 4634 5421 5689 1239 Size: 831 597 787 268 1524 Fragments arranged by size: 1524 960 831 787 597 393 268 214 144 140 66 26 24 EcoO109I rG'GnC_Cy Cuts at: 2068 2410 2556 2610 2750 2923 2068 Size: 342 146 54 140 173 5119 Fragments arranged by size: 5119 342 173 146 140 54 EcoRI G'AATT_C Cuts at: 2309 3283 3661 2309 Size: 974 378 4622 Fragments arranged by size: 4622 974 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2539 2539 Size: 5974 FspI TGC'GCA Cuts at: 4480 4480 Size: 5974 HaeII r_GCGC'y Cuts at: 1678 5121 1678 Size: 3443 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3179 3211 4615 4647 4689 Size: 32 135 32 1607 32 1404 32 42 Cuts at: 4689 4721 1373 Size: 32 2626 Fragments arranged by size: 2626 1607 1404 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3318 3564 669 Size: 988 281 1380 246 3079 Fragments arranged by size: 3079 1380 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5974 HpaI GTT'AAC Cuts at: 3564 3564 Size: 5974 KpnI G_GTAC'C Cuts at: 3299 3299 Size: 5974 MfeI C'AATT_G Cuts at: 3573 3573 Size: 5974 MluI A'CGCG_T Cuts at: 5850 5850 Size: 5974 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5088 5272 1305 Size: 3783 184 2007 Fragments arranged by size: 3783 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2168 2366 2888 10 Size: 54 1425 343 336 198 522 3096 Fragments arranged by size: 3096 1425 522 343 336 198 54 MslI CAynn'nnrTG Cuts at: 518 1166 2741 3059 3080 3932 4291 4450 518 Size: 648 1575 318 21 852 359 159 2042 Fragments arranged by size: 2042 1575 852 648 359 318 159 21 MspA1I CmG'CkG Cuts at: 1606 2198 2239 2339 2538 2854 3390 4067 5215 Size: 592 41 100 199 316 536 677 1148 Cuts at: 5215 5460 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 592 536 316 245 199 100 41 NaeI GCC'GGC Cuts at: 3021 3021 Size: 5974 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4483 Fragments arranged by size: 4483 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5974 NgoMIV G'CCGG_C Cuts at: 3019 3019 Size: 5974 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5974 NotI GC'GGCC_GC Cuts at: 3421 3421 Size: 5974 NspI r_CATG'y Cuts at: 1132 1229 3332 4877 1132 Size: 97 2103 1545 2229 Fragments arranged by size: 2229 2103 1545 97 PasI CC'CwG_GG Cuts at: 1348 2064 2927 1348 Size: 716 863 4395 Fragments arranged by size: 4395 863 716 PciI A'CATG_T Cuts at: 4873 4873 Size: 5974 PflMI CCAn_nnn'nTGG Cuts at: 1108 2362 2746 3140 1108 Size: 1254 384 394 3942 Fragments arranged by size: 3942 1254 394 384 PmeI GTTT'AAAC Cuts at: 3278 3278 Size: 5974 PmlI CAC'GTG Cuts at: 2299 2299 Size: 5974 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2091 2123 4043 4075 5556 5588 2091 Size: 32 1920 32 1481 32 2477 Fragments arranged by size: 2477 1920 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2410 2556 2610 2923 2410 Size: 146 54 313 5461 Fragments arranged by size: 5461 313 146 54 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5974 PsiI TTA'TAA Cuts at: 2592 3544 2592 Size: 952 5022 PspOMI G'GGCC_C Cuts at: 2067 2750 2067 Size: 683 5291 PstI C_TGCA'G Cuts at: 830 2721 3326 830 Size: 1891 605 3478 Fragments arranged by size: 3478 1891 605 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5974 PvuII CAG'CTG Cuts at: 2538 2538 Size: 5974 SalI G'TCGA_C Cuts at: 1936 3316 1936 Size: 1380 4594 SanDI GG'GwC_CC Cuts at: 2556 2923 2556 Size: 367 5607 SbfI CC_TGCA'GG Cuts at: 3326 3326 Size: 5974 ScaI AGT'ACT Cuts at: 4222 5934 4222 Size: 1712 4262 SfcI C'TryA_G Cuts at: 826 1045 2533 2717 3322 4457 5138 5329 826 Size: 219 1488 184 605 1135 681 191 1471 Fragments arranged by size: 1488 1471 1135 681 605 219 191 184 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5974 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5974 SmaI CCC'GGG Cuts at: 1872 2305 3301 1872 Size: 433 996 4545 Fragments arranged by size: 4545 996 433 SmlI C'TyrA_G Cuts at: 820 1017 1943 2513 2834 4078 4979 5241 820 Size: 197 926 570 321 1244 901 262 1553 Fragments arranged by size: 1553 1244 926 901 570 321 262 197 SnaBI TAC'GTA Cuts at: 493 3765 493 Size: 3272 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5974 SphI G_CATG'C Cuts at: 1132 3332 1132 Size: 2200 3774 SspI AAT'ATT Cuts at: 5 52 3898 5 Size: 47 3846 2081 Fragments arranged by size: 3846 2081 47 StuI AGG'CCT Cuts at: 1517 2091 1517 Size: 574 5400 StyI C'CwwG_G Cuts at: 513 1065 2004 2169 3413 513 Size: 552 939 165 1244 3074 Fragments arranged by size: 3074 1244 939 552 165 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2438 4001 4018 4171 4356 4801 325 Size: 1255 858 1563 17 153 185 445 1498 Fragments arranged by size: 1563 1498 1255 858 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3078 4220 5932 Size: 274 80 33 51 1443 1101 1142 1712 Cuts at: 5932 96 Size: 138 Fragments arranged by size: 1712 1443 1142 1101 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2127 2159 2852 2864 2884 2896 5934 5966 2127 Size: 32 693 12 20 12 3038 32 2135 Fragments arranged by size: 3038 2135 693 32 32 20 12 12 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5974 XbaI T'CTAG_A Cuts at: 3310 3310 Size: 5974 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2137 2374 3167 2137 Size: 237 793 4944 Fragments arranged by size: 4944 793 237 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5974 XmaI C'CCGG_G Cuts at: 1870 2303 3299 1870 Size: 433 996 4545 Fragments arranged by size: 4545 996 433 XmnI GAAnn'nnTTC Cuts at: 2727 3213 4103 2727 Size: 486 890 4598 Fragments arranged by size: 4598 890 486 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3782 276 Size: 53 83 186 936 2248 2468 Fragments arranged by size: 2468 2248 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BbeI BbvCI BfrBI Bpu10I BsiWI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PfoI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI