(Circular) (Six-base) MAPSORT of: pFN21AE0344.seq Check: 5763 from: 1 to: 6826 pFN21AE0344 6826 bp With 182 enzymes: SgfI * August 16, 2010 14:42 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2670 2767 2670 Size: 97 6729 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4636 278 Size: 53 83 186 936 3100 2468 Fragments arranged by size: 3100 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2973 4169 1937 Size: 1036 1196 4594 Fragments arranged by size: 4594 1196 1036 Acc65I G'GTAC_C Cuts at: 4147 4147 Size: 6826 AclI AA'CG_TT Cuts at: 1563 4953 5326 1563 Size: 3390 373 3063 Fragments arranged by size: 3390 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3107 4889 6273 6541 1632 Size: 1475 1782 1384 268 1917 Fragments arranged by size: 1917 1782 1475 1384 268 AfeI AGC'GCT Cuts at: 3686 3686 Size: 6826 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6629 AflIII A'CryG_T Cuts at: 1203 5725 6702 1203 Size: 4522 977 1327 Fragments arranged by size: 4522 1327 977 AgeI A'CCGG_T Cuts at: 5657 5657 Size: 6826 AhdI GACnn_n'nnGTC Cuts at: 5555 5555 Size: 6826 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6826 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2895 1184 Size: 1711 5115 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3434 3466 3434 Size: 32 6794 AlwNI CAG_nnn'CTG Cuts at: 2173 2236 2574 2629 2782 2794 2875 3085 3967 Size: 63 338 55 153 12 81 210 882 Cuts at: 3967 4569 6141 2173 Size: 602 1572 2858 Fragments arranged by size: 2858 1572 882 602 338 210 153 81 63 55 12 ApaI G_GGCC'C Cuts at: 2485 3313 3548 2485 Size: 828 235 5763 Fragments arranged by size: 5763 828 235 ApaLI G'TGCA_C Cuts at: 2259 2999 4883 6039 2259 Size: 740 1884 1156 3046 Fragments arranged by size: 3046 1884 1156 740 ApoI r'AATT_y Cuts at: 1493 4135 4361 4513 6622 1493 Size: 2642 226 152 2109 1697 Fragments arranged by size: 2642 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5380 160 Size: 872 4348 1606 Fragments arranged by size: 4348 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6826 AvaI C'yCGr_G Cuts at: 1870 1943 2111 3281 4151 1870 Size: 73 168 1170 870 4545 Fragments arranged by size: 4545 1170 870 168 73 BamHI G'GATC_C Cuts at: 4156 4523 4156 Size: 367 6459 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3259 3294 3326 3496 3735 Size: 325 217 627 1472 35 32 170 239 Cuts at: 3735 4003 4147 5602 618 Size: 268 144 1455 1842 Fragments arranged by size: 1842 1472 1455 627 325 268 239 217 170 144 35 32 BanII G_rGCy'C Cuts at: 1391 2015 2281 2294 2485 2595 3282 3313 3548 Size: 624 266 13 191 110 687 31 235 Cuts at: 3548 3682 3930 4145 1391 Size: 134 248 215 4072 Fragments arranged by size: 4072 687 624 266 248 235 215 191 134 110 31 13 BbeI G_GCGC'C Cuts at: 3298 3739 3298 Size: 441 6385 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2050 2502 2915 3095 928 Size: 971 151 452 413 180 4659 Fragments arranged by size: 4659 971 452 413 180 151 BbvCI CC'TCA_GC Cuts at: 2432 2432 Size: 6826 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5017 5051 1903 Size: 34 3080 34 3678 Fragments arranged by size: 3678 3080 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3654 4718 5928 3654 Size: 1064 1210 4552 Fragments arranged by size: 4552 1210 1064 BclI T'GATC_A Cuts at: 1548 1548 Size: 6826 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2106 2115 2355 Size: 107 122 71 631 605 434 9 240 Cuts at: 2355 2671 2939 3944 5437 136 Size: 316 268 1005 1493 1525 Fragments arranged by size: 1525 1493 1005 631 605 434 316 268 240 122 107 71 9 BglII A'GATC_T Cuts at: 6821 6821 Size: 6826 BlpI GC'TnA_GC Cuts at: 2246 2288 2684 2883 4243 2246 Size: 42 396 199 1360 4829 Fragments arranged by size: 4829 1360 396 199 42 Bme1580I G_kGCm'C Cuts at: 1790 2263 2485 3003 3154 3227 3313 3501 3548 Size: 473 222 518 151 73 86 188 47 Cuts at: 3548 4887 6043 1790 Size: 1339 1156 2573 Fragments arranged by size: 2573 1339 1156 518 473 222 188 151 86 73 47 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6826 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2222 2617 3390 Size: 416 53 83 399 24 805 395 773 Cuts at: 3390 4557 5515 442 Size: 1167 958 1753 Fragments arranged by size: 1753 1167 958 805 773 416 399 395 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3974 1056 Size: 2918 3908 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2024 2258 3031 3035 3431 3809 3923 4078 Size: 785 234 773 4 396 378 114 155 Cuts at: 4078 5486 1239 Size: 1408 2579 Fragments arranged by size: 2579 1408 785 773 396 378 234 155 114 4 Bpu10I CC'TnA_GC Cuts at: 2432 3473 3800 2432 Size: 1041 327 5458 Fragments arranged by size: 5458 1041 327 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4951 5816 6114 4951 Size: 865 298 5663 Fragments arranged by size: 5663 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2174 2612 5489 882 Size: 617 675 438 2877 2219 Fragments arranged by size: 2877 2219 675 617 438 BsaAI yAC'GTr Cuts at: 493 1204 3274 4617 493 Size: 711 2070 1343 2702 Fragments arranged by size: 2702 2070 1343 711 BsaBI GATnn'nnATC Cuts at: 2801 6820 2801 Size: 4019 2807 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2749 3295 3736 4633 Size: 53 83 186 936 1216 546 441 897 Cuts at: 4633 5015 5650 275 Size: 382 635 1451 Fragments arranged by size: 1451 1216 936 897 635 546 441 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3987 4049 5259 5657 5931 6078 6489 3987 Size: 62 1210 398 274 147 411 4324 Fragments arranged by size: 4324 1210 411 398 274 147 62 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3434 3464 3686 3716 4076 Size: 30 165 30 2031 30 222 30 360 Cuts at: 4076 4106 1178 Size: 30 3898 Fragments arranged by size: 3898 2031 360 222 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3764 3920 1186 Size: 2578 156 4092 Fragments arranged by size: 4092 2578 156 BseYI C'CCAG_C Cuts at: 2254 3115 3681 3705 6029 6525 2254 Size: 861 566 24 2324 496 2555 Fragments arranged by size: 2555 2324 861 566 496 24 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2660 3980 1163 Size: 573 924 1320 4009 Fragments arranged by size: 4009 1320 924 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2055 3234 3482 4276 5037 6065 Size: 275 186 54 1179 248 794 761 1028 Cuts at: 6065 1540 Size: 2301 Fragments arranged by size: 2301 1179 1028 794 761 275 248 186 54 BsiHKAI G_wGCw'C Cuts at: 2263 3003 3339 4887 4972 6043 2263 Size: 740 336 1548 85 1071 3046 Fragments arranged by size: 3046 1548 1071 740 336 85 BsmI GAATG_Cn' Cuts at: 4337 4430 4337 Size: 93 6733 BsmBI CGTCTCn'nnnn_ Cuts at: 3785 3785 Size: 6826 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2015 2263 2281 2294 2485 2595 3003 Size: 399 225 248 18 13 191 110 408 Cuts at: 3003 3154 3227 3282 3313 3339 3501 3548 3682 Size: 151 73 55 31 26 162 47 134 Cuts at: 3682 3930 4145 4887 4972 6043 1391 Size: 248 215 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 408 399 248 248 225 215 191 162 151 134 110 85 73 55 47 31 26 18 13 BspEI T'CCGG_A Cuts at: 4049 6489 4049 Size: 2440 4386 BspHI T'CATG_A Cuts at: 4715 6445 4715 Size: 1730 5096 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2670 2767 2779 4181 844 Size: 1826 97 12 1402 3489 Fragments arranged by size: 3489 1826 1402 97 12 BsrBI CCG'CTC Cuts at: 1394 1823 4713 1394 Size: 429 2890 3507 Fragments arranged by size: 3507 2890 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5321 5495 65 Size: 1183 4073 174 1396 Fragments arranged by size: 4073 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2665 2807 3477 5470 5657 1700 Size: 110 855 142 670 1993 187 2869 Fragments arranged by size: 2869 1993 855 670 187 142 110 BsrGI T'GTAC_A Cuts at: 96 3456 96 Size: 3360 3466 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6826 BssSI C'ACGA_G Cuts at: 4886 5898 4886 Size: 1012 5814 BstAPI GCAn_nnn'nTGC Cuts at: 794 2782 3967 794 Size: 1988 1185 3653 Fragments arranged by size: 3653 1988 1185 BstBI TT'CG_AA Cuts at: 5630 5630 Size: 6826 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6826 BstYI r'GATC_y Cuts at: 1972 4156 4523 4908 4925 6377 6463 6475 6821 Size: 2184 367 385 17 1452 86 12 346 Cuts at: 6821 1972 Size: 1977 Fragments arranged by size: 2184 1977 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2148 2548 3399 2148 Size: 400 851 5575 Fragments arranged by size: 5575 851 400 BtgI C'CryG_G Cuts at: 513 1065 2004 2024 2893 3234 3289 3330 3563 Size: 552 939 20 869 341 55 41 233 Cuts at: 3563 3939 513 Size: 376 3400 Fragments arranged by size: 3400 939 869 552 376 341 233 55 41 20 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2533 487 Size: 683 56 1307 4780 Fragments arranged by size: 4780 1307 683 56 BtsI GCAGTG_nn' Cuts at: 2294 2595 2850 3225 4342 5136 5156 2294 Size: 301 255 375 1117 794 20 3964 Fragments arranged by size: 3964 1117 794 375 301 255 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6826 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6791 DraI TTT'AAA Cuts at: 4130 4130 Size: 6826 DraIII CAC_nnn'GTG Cuts at: 3111 3111 Size: 6826 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5833 809 Size: 507 4517 1802 Fragments arranged by size: 4517 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2052 2412 2694 Size: 54 1425 50 275 18 222 360 282 Cuts at: 2694 2890 2913 2940 3348 3427 3479 3636 3936 Size: 196 23 27 408 79 52 157 300 Cuts at: 3936 4273 5162 6791 8 Size: 337 889 1629 43 Fragments arranged by size: 1629 1425 889 408 360 337 300 282 275 222 196 157 79 54 52 50 43 27 23 18 EagI C'GGCC_G Cuts at: 1537 1812 2052 3479 4273 1537 Size: 275 240 1427 794 4090 Fragments arranged by size: 4090 1427 794 275 240 EarI CTCTTCn'nnn_ Cuts at: 1353 2039 3371 4756 1353 Size: 686 1332 1385 3423 Fragments arranged by size: 3423 1385 1332 686 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2522 3359 3605 5413 5787 5933 Size: 1064 138 933 837 246 1808 374 146 Cuts at: 5933 387 Size: 1280 Fragments arranged by size: 1808 1280 1064 933 837 374 246 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2024 2258 3031 3035 3107 3431 3809 Size: 393 392 234 773 4 72 324 378 Cuts at: 3809 3923 4078 4889 5486 6273 6541 1239 Size: 114 155 811 597 787 268 1524 Fragments arranged by size: 1524 811 787 773 597 393 392 378 324 268 234 155 114 72 4 EcoNI CCTnn'n_nnAGG Cuts at: 3593 3863 3593 Size: 270 6556 EcoO109I rG'GnC_Cy Cuts at: 2233 2458 2481 2482 2791 2932 3285 3309 3778 Size: 225 23 1 309 141 353 24 469 Cuts at: 3778 3868 3907 3964 2233 Size: 90 39 57 5095 Fragments arranged by size: 5095 469 353 309 225 141 90 57 39 24 23 1 EcoRI G'AATT_C Cuts at: 4135 4513 4135 Size: 378 6448 FspI TGC'GCA Cuts at: 5332 5332 Size: 6826 HaeII r_GCGC'y Cuts at: 1678 2164 2207 3298 3688 3739 3849 5973 1678 Size: 486 43 1091 390 51 110 2124 2531 Fragments arranged by size: 2531 2124 1091 486 390 110 51 43 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3871 3903 5467 5499 5541 Size: 32 135 32 2299 32 1564 32 42 Cuts at: 5541 5573 1373 Size: 32 2626 Fragments arranged by size: 2626 2299 1564 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3022 4170 4416 669 Size: 988 281 1084 1148 246 3079 Fragments arranged by size: 3079 1148 1084 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6826 HpaI GTT'AAC Cuts at: 4416 4416 Size: 6826 KasI G'GCGC_C Cuts at: 3294 3735 3294 Size: 441 6385 KpnI G_GTAC'C Cuts at: 4151 4151 Size: 6826 MfeI C'AATT_G Cuts at: 4425 4425 Size: 6826 MluI A'CGCG_T Cuts at: 6702 6702 Size: 6826 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2841 3232 5940 6124 1305 Size: 1536 391 2708 184 2007 Fragments arranged by size: 2708 2007 1536 391 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2696 2892 2942 3350 3638 Size: 54 1425 343 864 196 50 408 288 Cuts at: 3638 10 Size: 3198 Fragments arranged by size: 3198 1425 864 408 343 288 196 54 50 MslI CAynn'nnrTG Cuts at: 518 1166 3938 4784 5143 5302 518 Size: 648 2772 846 359 159 2042 Fragments arranged by size: 2772 2042 846 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2026 2173 2431 2574 3346 3892 4000 4014 Size: 420 147 258 143 772 546 108 14 Cuts at: 4014 4242 4919 6067 6312 1606 Size: 228 677 1148 245 2120 Fragments arranged by size: 2120 1148 772 677 546 420 258 245 228 147 143 108 14 NaeI GCC'GGC Cuts at: 3479 3479 Size: 6826 NarI GG'CG_CC Cuts at: 3295 3736 3295 Size: 441 6385 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5335 Fragments arranged by size: 5335 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6826 NgoMIV G'CCGG_C Cuts at: 3477 3477 Size: 6826 NheI G'CTAG_C Cuts at: 1052 3970 1052 Size: 2918 3908 NotI GC'GGCC_GC Cuts at: 4273 4273 Size: 6826 NspI r_CATG'y Cuts at: 1132 1229 4184 5729 1132 Size: 97 2955 1545 2229 Fragments arranged by size: 2955 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2230 2272 2464 3541 3772 1348 Size: 882 42 192 1077 231 4402 Fragments arranged by size: 4402 1077 882 231 192 42 PciI A'CATG_T Cuts at: 5725 5725 Size: 6826 PflMI CCAn_nnn'nTGG Cuts at: 1108 2047 2236 2875 2922 3111 3745 1108 Size: 939 189 639 47 189 634 4189 Fragments arranged by size: 4189 939 639 634 189 189 47 PmeI GTTT'AAAC Cuts at: 4130 4130 Size: 6826 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3434 3466 4895 4927 6408 6440 3434 Size: 32 1429 32 1481 32 3820 Fragments arranged by size: 3820 1481 1429 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2791 3285 3778 2791 Size: 494 493 5839 Fragments arranged by size: 5839 494 493 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6826 PsiI TTA'TAA Cuts at: 4396 4396 Size: 6826 PspOMI G'GGCC_C Cuts at: 2481 3309 3544 2481 Size: 828 235 5763 Fragments arranged by size: 5763 828 235 PstI C_TGCA'G Cuts at: 830 2790 3597 4178 830 Size: 1960 807 581 3478 Fragments arranged by size: 3478 1960 807 581 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6826 PvuII CAG'CTG Cuts at: 2173 3346 3892 2173 Size: 1173 546 5107 Fragments arranged by size: 5107 1173 546 SacII CC_GC'GG Cuts at: 2027 2027 Size: 6826 SalI G'TCGA_C Cuts at: 1936 4168 1936 Size: 2232 4594 SanDI GG'GwC_CC Cuts at: 3285 3778 3285 Size: 493 6333 SapI GCTCTTCn'nnn_ Cuts at: 2039 2039 Size: 6826 SbfI CC_TGCA'GG Cuts at: 4178 4178 Size: 6826 ScaI AGT'ACT Cuts at: 3695 5074 6786 3695 Size: 1379 1712 3735 Fragments arranged by size: 3735 1712 1379 SfcI C'TryA_G Cuts at: 826 1045 2786 3201 3341 3593 3815 4174 5309 Size: 219 1741 415 140 252 222 359 1135 Cuts at: 5309 5990 6181 826 Size: 681 191 1471 Fragments arranged by size: 1741 1471 1135 681 415 359 252 222 219 191 140 SfiI GGCCn_nnn'nGGCC Cuts at: 2106 2939 2106 Size: 833 5993 SfoI GGC'GCC Cuts at: 3296 3737 3296 Size: 441 6385 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6826 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6826 SmaI CCC'GGG Cuts at: 1872 3283 4153 1872 Size: 1411 870 4545 Fragments arranged by size: 4545 1411 870 SmlI C'TyrA_G Cuts at: 820 1017 1943 4930 5831 6093 820 Size: 197 926 2987 901 262 1553 Fragments arranged by size: 2987 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4617 493 Size: 4124 2702 SpeI A'CTAG_T Cuts at: 152 2263 152 Size: 2111 4715 SphI G_CATG'C Cuts at: 1132 4184 1132 Size: 3052 3774 SspI AAT'ATT Cuts at: 5 52 4750 5 Size: 47 4698 2081 Fragments arranged by size: 4698 2081 47 StuI AGG'CCT Cuts at: 1517 2100 1517 Size: 583 6243 StyI C'CwwG_G Cuts at: 513 1065 2004 2928 4265 513 Size: 552 939 924 1337 3074 Fragments arranged by size: 3074 1337 939 924 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2246 3107 3733 4853 4870 5023 5208 Size: 1255 666 861 626 1120 17 153 185 Cuts at: 5208 5653 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1120 861 666 626 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3456 3693 5072 Size: 274 80 33 51 1443 1479 237 1379 Cuts at: 5072 6784 96 Size: 1712 138 Fragments arranged by size: 1712 1479 1443 1379 274 237 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3181 3213 6786 6818 3181 Size: 32 3573 32 3189 Fragments arranged by size: 3573 3189 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6826 XbaI T'CTAG_A Cuts at: 4162 4162 Size: 6826 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2857 2935 3112 3320 3516 3856 2857 Size: 78 177 208 196 340 5827 Fragments arranged by size: 5827 340 208 196 177 78 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6826 XmaI C'CCGG_G Cuts at: 1870 3281 4151 1870 Size: 1411 870 4545 Fragments arranged by size: 4545 1411 870 XmnI GAAnn'nnTTC Cuts at: 4955 4955 Size: 6826 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4634 276 Size: 53 83 186 936 3100 2468 Fragments arranged by size: 3100 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BfrBI BplI BsiWI BstXI BstZ17I EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI SexAI SrfI SwaI