Restriction map for
pFN21AF0021
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# NO CUT ENZYMES ( 108 enzymes) #
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Acc65I AclI AcsI AflII Alw44I
AocI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspHI
AvrII BalI BamHI BbeI BbrPI
BcgI BciVI BclI BfaI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsiEI BsiHKAI BsiWI BspDI BssSI
Bst1107I BstBI BstZ17I Bsu36I CelII
ClaI DraI DraIII DrdI EagI
EclXI Eco47III Eco52I Eco81I EcoRI
EcoRV EcoT22I FbaI FseI HindIII
HpaI KasI KpnI MaeI MfeI
MluI MluNI MscI MunI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI SacI SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspI StuI SwaI
TaqI TfiI TthHB8I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccI 841 GTCTAC GT^[AC][GT]AC
AccIII 818 TCCGGA T^CCGGA
AgeI 587 ACCGGT A^CCGGT
AhdI 1249 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 700 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 598 GGGCCC GGGCC^C
AspEI 1249 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1186 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 810 CTCGGG C^[CT]CG[GA]G
AviII 1127 TGCGCA TGC^GCA
BanII 598 GGGCCC G[GA]GC[CT]^C
BbvCI 1470 CCTCAGC CCTCAGC(-5/-2)
BpmI 1252 CTGGAG CTGGAG(16/14)
BsaBI 759 GATCCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 818 TCCGGA T^CCGGA
BsmI 106 GCATTC GAATGC(1/-1)
BsoBI 810 CTCGGG C^[CT]CG[GA]G
BspEI 818 TCCGGA T^CCGGA
BsrBI 313 GAGCGG CCGCTC(-3/-3)
BssHII 1027 GCGCGC G^CGCGC
BstAPI 861 GCACTCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 519 GGTGACC G^GT[ACGT]ACC
BstPI 519 GGTGACC G^GT[ACGT]ACC
BstXI 1205 CCATCACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CpoI 657 CGGACCG CG^G[AT]CCG
Eam1105I 1249 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 519 GGTGACC G^GT[ACGT]ACC
FspI 1127 TGCGCA TGC^GCA
HincII 433 GTCAAC GT[CT]^[GA]AC
HindII 433 GTCAAC GT[CT]^[GA]AC
KspI 987 CCGCGG CCGC^GG
MamI 759 GATCCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 818 TCCGGA T^CCGGA
MseI 65 TTAA T^TAA
NcoI 197 CCATGG C^CATGG
PflFI 1186 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1245 CCACGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 587 ACCGGT A^CCGGT
PpuMI 1384 AGGTCCC [GA]G^G[AT]CC[CT]
PvuII 1348 CAGCTG CAG^CTG
RsrII 657 CGGACCG CG^G[AT]CCG
SacII 987 CCGCGG CCGC^GG
SmlI 244 CTCAAG C^T[CT][GA]AG
Tru9I 65 TTAA T^TAA
Tsp509I 12 AATT ^AATT
Tth111I 1186 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1245 CCACGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 26 enzymes) #
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AatII 676 GACGTC GACGT^C
1036 GACGTC
AflIII 59 ACATGT A^C[GA][CT]GT
1007 ACATGT
BsaAI 931 TACGTG [CT]AC^GT[GA]
1167 CACGTA
BsaWI 587 ACCGGT [AT]^CCGG[AT]
818 TCCGGA
BsgI 1239 CTGCAC GTGCAG(16/14)
343 GTGCAG
BsmBI 888 CGTCTC CGTCTC(1/5)
348 GAGACG
Bsp1407I 56 TGTACA T^GTACA
1010 TGTACA
BspHI 161 TCATGA T^CATGA
740 TCATGA
BspLU11I 59 ACATGT A^CATGT
1007 ACATGT
BsrDI 1113 CATTGC GCAATG(2/0)
539 GCAATG
BsrFI 587 ACCGGT [GA]^CCGG[CT]
1291 ACCGGC
BsrGI 56 TGTACA T^GTACA
1010 TGTACA
BstYI 355 GGATCT [GA]^GATC[CT]
701 AGATCC
Cfr10I 587 ACCGGT [GA]^CCGG[CT]
1291 ACCGGC
DdeI 279 CTCAG C^T[ACGT]AG
1471 CTCAG
EaeI 1049 TGGCCG [CT]^GGCC[GA]
1457 CGGCCA
EcoNI 243 CCTCAAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
510 CCTCATGCAGG
EcoT14I 101 CCAAGG C^C[AT][AT]GG
197 CCATGG
HaeII 529 AGCGCC [GA]GCGC^[CT]
899 AGCGCC
MflI 355 GGATCT [GA]^GATC[CT]
701 AGATCC
NspI 59 ACATGT [GA]CATG^[CT]
1007 ACATGT
RcaI 161 TCATGA T^CATGA
740 TCATGA
SspBI 56 TGTACA T^GTACA
1010 TGTACA
StyI 101 CCAAGG C^C[AT][AT]GG
197 CCATGG
XcmI 1202 CCACCATCACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1347 CCAGCTGGCCTTTGG
XhoII 355 GGATCT [GA]^GATC[CT]
701 AGATCC
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# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AvaII BanI BbsI BbvI
BcnI BmyI BpuAI BsaHI BsaI
BsaJI BseRI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DpnII DraII DsaI EarI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MnlI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PleI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI TseI
Tsp45I TspRI