(Circular) (Six-base) MAPSORT of: pFN21AF0021.seq Check: 3489 from: 1 to: 6193 pFN21AF0021 6193 bp With 182 enzymes: SgfI * October 20, 2009 10:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2512 2512 Size: 6193 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2685 3045 4003 278 Size: 53 83 186 936 1149 360 958 2468 Fragments arranged by size: 2468 1149 958 936 360 186 83 53 AccI GT'mk_AC Cuts at: 1937 2847 3536 1937 Size: 910 689 4594 Fragments arranged by size: 4594 910 689 Acc65I G'GTAC_C Cuts at: 3514 3514 Size: 6193 AclI AA'CG_TT Cuts at: 1563 4320 4693 1563 Size: 2757 373 3063 Fragments arranged by size: 3063 2757 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4256 5640 5908 1632 Size: 2624 1384 268 1917 Fragments arranged by size: 2624 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5996 AflIII A'CryG_T Cuts at: 1203 2064 3012 5092 6069 1203 Size: 861 948 2080 977 1327 Fragments arranged by size: 2080 1327 977 948 861 AgeI A'CCGG_T Cuts at: 2592 5024 2592 Size: 2432 3761 AhdI GACnn_n'nnGTC Cuts at: 3259 4922 3259 Size: 1663 4530 AleI CACnn'nnGTG Cuts at: 1166 2189 3218 1166 Size: 1023 1029 4141 Fragments arranged by size: 4141 1029 1023 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6193 AlwNI CAG_nnn'CTG Cuts at: 2710 3936 5508 2710 Size: 1226 1572 3395 Fragments arranged by size: 3395 1572 1226 ApaI G_GGCC'C Cuts at: 2607 2607 Size: 6193 ApaLI G'TGCA_C Cuts at: 4250 5406 4250 Size: 1156 5037 ApoI r'AATT_y Cuts at: 1493 3502 3728 3880 5989 1493 Size: 2009 226 152 2109 1697 Fragments arranged by size: 2109 2009 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4747 160 Size: 872 3715 1606 Fragments arranged by size: 3715 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6193 AvaI C'yCGr_G Cuts at: 1870 1943 2815 3518 1870 Size: 73 872 703 4545 Fragments arranged by size: 4545 872 703 73 BamHI G'GATC_C Cuts at: 3523 3890 3523 Size: 367 5826 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2793 3220 3293 3514 4969 Size: 325 217 627 1006 427 73 221 1455 Cuts at: 4969 618 Size: 1842 Fragments arranged by size: 1842 1455 1006 627 427 325 221 217 73 BanII G_rGCy'C Cuts at: 1391 2607 3512 1391 Size: 1216 905 4072 Fragments arranged by size: 4072 1216 905 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2696 3082 3152 928 Size: 971 797 386 70 3969 Fragments arranged by size: 3969 971 797 386 70 BbvCI CC'TCA_GC Cuts at: 3476 3476 Size: 6193 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4384 4418 1903 Size: 34 2447 34 3678 Fragments arranged by size: 3678 2447 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4085 5295 4085 Size: 1210 4983 BclI T'GATC_A Cuts at: 1548 1548 Size: 6193 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4804 136 Size: 107 122 71 631 605 3132 1525 Fragments arranged by size: 3132 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6188 6188 Size: 6193 BlpI GC'TnA_GC Cuts at: 3610 3610 Size: 6193 Bme1580I G_kGCm'C Cuts at: 1790 2087 2508 2607 2778 2796 3296 4254 5410 Size: 297 421 99 171 18 500 958 1156 Cuts at: 5410 1790 Size: 2573 Fragments arranged by size: 2573 1156 958 500 421 297 171 99 18 BmgBI CAC'GTC Cuts at: 1318 2357 1318 Size: 1039 5154 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3924 4882 442 Size: 416 53 83 399 24 2507 958 1753 Fragments arranged by size: 2507 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6193 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3278 4853 1239 Size: 2039 1575 2579 Fragments arranged by size: 2579 2039 1575 Bpu10I CC'TnA_GC Cuts at: 3476 3476 Size: 6193 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2234 4318 5183 5481 2234 Size: 2084 865 298 2946 Fragments arranged by size: 2946 2084 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2018 2472 3476 4856 882 Size: 617 519 454 1004 1380 2219 Fragments arranged by size: 2219 1380 1004 617 519 454 BsaAI yAC'GTr Cuts at: 493 1204 2938 3174 3984 493 Size: 711 1734 236 810 2702 Fragments arranged by size: 2702 1734 810 711 236 BsaBI GATnn'nnATC Cuts at: 2768 6187 2768 Size: 3419 2774 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2622 2682 3042 4000 Size: 53 83 186 936 1089 60 360 958 Cuts at: 4000 4382 5017 275 Size: 382 635 1451 Fragments arranged by size: 1451 1089 958 936 635 382 360 186 83 60 53 BsaWI w'CCGG_w Cuts at: 2592 2823 4626 5024 5298 5445 5856 2592 Size: 231 1803 398 274 147 411 2929 Fragments arranged by size: 2929 1803 411 398 274 231 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2258 2288 1178 Size: 30 165 30 855 30 5083 Fragments arranged by size: 5083 855 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2263 2270 2645 2648 2887 2954 1186 Size: 1077 7 375 3 239 67 4425 Fragments arranged by size: 4425 1077 375 239 67 7 3 BseYI C'CCAG_C Cuts at: 2598 3351 5396 5892 2598 Size: 753 2045 496 2899 Fragments arranged by size: 2899 2045 753 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2369 3229 1163 Size: 573 633 860 4127 Fragments arranged by size: 4127 860 633 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3643 4404 5432 1540 Size: 275 186 1642 761 1028 2301 Fragments arranged by size: 2301 1642 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4254 4339 5410 4254 Size: 85 1071 5037 Fragments arranged by size: 5037 1071 85 BsmI GAATG_Cn' Cuts at: 2111 3704 3797 2111 Size: 1593 93 4507 Fragments arranged by size: 4507 1593 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2347 2899 2347 Size: 552 5641 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2087 2508 2607 2778 2796 3296 3512 Size: 399 297 421 99 171 18 500 216 Cuts at: 3512 4254 4339 5410 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 500 421 399 297 216 171 99 85 18 BspEI T'CCGG_A Cuts at: 2823 5856 2823 Size: 3033 3160 BspHI T'CATG_A Cuts at: 2166 2745 4082 5812 2166 Size: 579 1337 1730 2547 Fragments arranged by size: 2547 1730 1337 579 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2512 2537 2709 3548 844 Size: 1668 25 172 839 3489 Fragments arranged by size: 3489 1668 839 172 25 BsrBI CCG'CTC Cuts at: 1394 1823 2320 4080 1394 Size: 429 497 1760 3507 Fragments arranged by size: 3507 1760 497 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2551 3117 4688 4862 65 Size: 1183 1303 566 1571 174 1396 Fragments arranged by size: 1571 1396 1303 1183 566 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2592 3296 4837 5024 1700 Size: 110 782 704 1541 187 2869 Fragments arranged by size: 2869 1541 782 704 187 110 BsrGI T'GTAC_A Cuts at: 96 2061 3015 96 Size: 1965 954 3274 Fragments arranged by size: 3274 1965 954 BssHII G'CGCG_C Cuts at: 1925 3032 1925 Size: 1107 5086 BssSI C'ACGA_G Cuts at: 4253 5265 4253 Size: 1012 5181 BstAPI GCAn_nnn'nTGC Cuts at: 794 2872 794 Size: 2078 4115 BstBI TT'CG_AA Cuts at: 4997 4997 Size: 6193 BstEII G'GTnAC_C Cuts at: 1184 2524 1184 Size: 1340 4853 BstXI CCAn_nnnn'nTGG Cuts at: 3217 3217 Size: 6193 BstYI r'GATC_y Cuts at: 1972 2360 2706 3523 3890 4275 4292 5744 5830 Size: 388 346 817 367 385 17 1452 86 Cuts at: 5830 5842 6188 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 817 388 385 367 346 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2202 2992 3179 513 Size: 552 939 198 790 187 3527 Fragments arranged by size: 3527 939 790 552 198 187 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2307 487 Size: 683 56 1081 4373 Fragments arranged by size: 4373 1081 683 56 BtsI GCAGTG_nn' Cuts at: 2118 3334 3709 4503 4523 2118 Size: 1216 375 794 20 3788 Fragments arranged by size: 3788 1216 794 375 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6193 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2170 2205 529 Size: 35 1606 35 4517 Fragments arranged by size: 4517 1606 35 35 DraI TTT'AAA Cuts at: 3497 3497 Size: 6193 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5200 809 Size: 507 3884 1802 Fragments arranged by size: 3884 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3054 3462 3640 Size: 54 1425 50 275 18 1224 408 178 Cuts at: 3640 4529 6158 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1224 889 408 275 178 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3640 1537 Size: 275 1828 4090 Fragments arranged by size: 4090 1828 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2047 2228 3086 3158 4123 1353 Size: 694 181 858 72 965 3423 Fragments arranged by size: 3423 965 858 694 181 72 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3389 4780 5154 5300 387 Size: 1064 138 1800 1391 374 146 1280 Fragments arranged by size: 1800 1391 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3278 4256 4853 5640 5908 1239 Size: 393 1646 978 597 787 268 1524 Fragments arranged by size: 1646 1524 978 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2252 2519 2252 Size: 267 5926 EcoO109I rG'GnC_Cy Cuts at: 2603 2604 3282 3390 2603 Size: 1 678 108 5406 Fragments arranged by size: 5406 678 108 1 EcoRI G'AATT_C Cuts at: 3502 3880 3502 Size: 378 5815 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3169 3169 Size: 6193 FspI TGC'GCA Cuts at: 3134 4699 3134 Size: 1565 4628 HaeII r_GCGC'y Cuts at: 1678 2538 2908 5340 1678 Size: 860 370 2432 2531 Fragments arranged by size: 2531 2432 860 370 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2255 2258 2287 2290 2635 Size: 32 135 32 683 3 29 3 345 Cuts at: 2635 2667 3245 3277 4834 4866 4908 4940 1373 Size: 32 578 32 1557 32 42 32 2626 Fragments arranged by size: 2626 1557 683 578 345 135 42 32 32 32 32 32 32 29 3 3 HincII GTy'rAC Cuts at: 669 1657 1938 2440 3537 3783 669 Size: 988 281 502 1097 246 3079 Fragments arranged by size: 3079 1097 988 502 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6193 HpaI GTT'AAC Cuts at: 3783 3783 Size: 6193 KpnI G_GTAC'C Cuts at: 3518 3518 Size: 6193 MfeI C'AATT_G Cuts at: 3792 3792 Size: 6193 MluI A'CGCG_T Cuts at: 6069 6069 Size: 6193 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5307 5491 1305 Size: 4002 184 2007 Fragments arranged by size: 4002 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4371 Fragments arranged by size: 4371 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2189 2522 2750 3011 3122 3218 4151 Size: 648 1023 333 228 261 111 96 933 Cuts at: 4151 4510 4669 518 Size: 359 159 2042 Fragments arranged by size: 2042 1023 933 648 359 333 261 228 159 111 96 MspA1I CmG'CkG Cuts at: 1606 2994 3059 3355 3609 4286 5434 5679 1606 Size: 1388 65 296 254 677 1148 245 2120 Fragments arranged by size: 2120 1388 1148 677 296 254 245 65 NcoI C'CATG_G Cuts at: 513 1065 2004 2202 513 Size: 552 939 198 4504 Fragments arranged by size: 4504 939 552 198 NdeI CA'TA_TG Cuts at: 387 387 Size: 6193 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6193 NotI GC'GGCC_GC Cuts at: 3640 3640 Size: 6193 NspI r_CATG'y Cuts at: 1132 1229 2068 3016 3551 5096 1132 Size: 97 839 948 535 1545 2229 Fragments arranged by size: 2229 1545 948 839 535 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6193 PciI A'CATG_T Cuts at: 2064 3012 5092 2064 Size: 948 2080 3165 Fragments arranged by size: 3165 2080 948 PflMI CCAn_nnn'nTGG Cuts at: 1108 3256 1108 Size: 2148 4045 PfoI T'CCnGG_A Cuts at: 2304 2928 2304 Size: 624 5569 PmeI GTTT'AAAC Cuts at: 3497 3497 Size: 6193 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4262 4294 5775 5807 4262 Size: 32 1481 32 4648 Fragments arranged by size: 4648 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3390 3390 Size: 6193 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6193 PsiI TTA'TAA Cuts at: 3763 3763 Size: 6193 PspOMI G'GGCC_C Cuts at: 2603 2603 Size: 6193 PstI C_TGCA'G Cuts at: 830 2212 2534 2706 3545 830 Size: 1382 322 172 839 3478 Fragments arranged by size: 3478 1382 839 322 172 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6193 PvuII CAG'CTG Cuts at: 3355 3355 Size: 6193 RsrII CG'GwC_CG Cuts at: 2663 2663 Size: 6193 SacII CC_GC'GG Cuts at: 2995 2995 Size: 6193 SalI G'TCGA_C Cuts at: 1936 3535 1936 Size: 1599 4594 SbfI CC_TGCA'GG Cuts at: 3545 3545 Size: 6193 ScaI AGT'ACT Cuts at: 4441 6153 4441 Size: 1712 4481 SfcI C'TryA_G Cuts at: 826 1045 2208 2530 2702 3541 4676 5357 5548 Size: 219 1163 322 172 839 1135 681 191 Cuts at: 5548 826 Size: 1471 Fragments arranged by size: 1471 1163 1135 839 681 322 219 191 172 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6193 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6193 SmaI CCC'GGG Cuts at: 1872 3520 1872 Size: 1648 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2249 4297 5198 5460 820 Size: 197 926 306 2048 901 262 1553 Fragments arranged by size: 2048 1553 926 901 306 262 197 SnaBI TAC'GTA Cuts at: 493 3984 493 Size: 3491 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6193 SphI G_CATG'C Cuts at: 1132 3551 1132 Size: 2419 3774 SspI AAT'ATT Cuts at: 5 52 4117 5 Size: 47 4065 2081 Fragments arranged by size: 4065 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6193 StyI C'CwwG_G Cuts at: 513 1065 2004 2106 2202 3632 513 Size: 552 939 102 96 1430 3074 Fragments arranged by size: 3074 1430 939 552 102 96 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3172 3263 3365 3403 4220 4237 4390 Size: 1255 1592 91 102 38 817 17 153 Cuts at: 4390 4575 5020 325 Size: 185 445 1498 Fragments arranged by size: 1592 1498 1255 817 445 185 153 102 91 38 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2061 3015 4439 Size: 274 80 33 51 1443 84 954 1424 Cuts at: 4439 6151 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1424 954 274 138 84 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6153 6185 6153 Size: 32 6161 Tth111I GACn'n_nGTC Cuts at: 1316 3194 1316 Size: 1878 4315 XbaI T'CTAG_A Cuts at: 3529 3529 Size: 6193 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3214 3359 3214 Size: 145 6048 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6193 XmaI C'CCGG_G Cuts at: 1870 3518 1870 Size: 1648 4545 XmnI GAAnn'nnTTC Cuts at: 4322 4322 Size: 6193 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2683 3043 4001 276 Size: 53 83 186 936 1149 360 958 2468 Fragments arranged by size: 2468 1149 958 936 360 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI SacI SanDI SapI SexAI SfiI SfoI SrfI SwaI