Restriction map for
pFN21AF0021
(Linear) (Six-base) MAPSORT of: FLJ00021.seq Check: 8982 from: 1 to: 1488
FLJ00021.orf
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 507 1488
Size: 507 981
AatII G_ACGT'C
Cuts at: 0 680 1040 1488
Size: 680 360 448
Fragments arranged by size:
680 448 360
AccI GT'mk_AC
Cuts at: 0 842 1488
Size: 842 646
AflIII A'CryG_T
Cuts at: 0 59 1007 1488
Size: 59 948 481
Fragments arranged by size:
948 481 59
AgeI A'CCGG_T
Cuts at: 0 587 1488
Size: 587 901
AhdI GACnn_n'nnGTC
Cuts at: 0 1254 1488
Size: 1254 234
AleI CACnn'nnGTG
Cuts at: 0 184 1213 1488
Size: 184 1029 275
Fragments arranged by size:
1029 275 184
AlwNI CAG_nnn'CTG
Cuts at: 0 705 1488
Size: 705 783
ApaI G_GGCC'C
Cuts at: 0 602 1488
Size: 602 886
AvaI C'yCGr_G
Cuts at: 0 810 1488
Size: 810 678
BanI G'GyrC_C
Cuts at: 0 788 1215 1288 1488
Size: 788 427 73 200
Fragments arranged by size:
788 427 200 73
BanII G_rGCy'C
Cuts at: 0 602 1488
Size: 602 886
BbsI GAAGACnn'nnnn_
Cuts at: 0 691 1077 1147 1488
Size: 691 386 70 341
Fragments arranged by size:
691 386 341 70
BbvCI CC'TCA_GC
Cuts at: 0 1471 1488
Size: 1471 17
Bme1580I G_kGCm'C
Cuts at: 0 82 503 602 773 791 1291 1488
Size: 82 421 99 171 18 500 197
Fragments arranged by size:
500 421 197 171 99 82 18
BmgBI CAC'GTC
Cuts at: 0 352 1488
Size: 352 1136
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1273 1488
Size: 1273 215
Bpu10I CC'TnA_GC
Cuts at: 0 1471 1488
Size: 1471 17
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 229 1488
Size: 229 1259
BsaI GGTCTCn'nnnn_
Cuts at: 0 13 467 1471 1488
Size: 13 454 1004 17
Fragments arranged by size:
1004 454 17 13
BsaAI yAC'GTr
Cuts at: 0 933 1169 1488
Size: 933 236 319
Fragments arranged by size:
933 319 236
BsaBI GATnn'nnATC
Cuts at: 0 763 1488
Size: 763 725
BsaHI Gr'CG_yC
Cuts at: 0 617 677 1037 1488
Size: 617 60 360 451
Fragments arranged by size:
617 451 360 60
BsaWI w'CCGG_w
Cuts at: 0 587 818 1488
Size: 587 231 670
Fragments arranged by size:
670 587 231
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 253 283 1488
Size: 253 30 1205
Fragments arranged by size:
1205 253 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 258 265 640 643 882 949 1488
Size: 258 7 375 3 239 67 539
Fragments arranged by size:
539 375 258 239 67 7 3
BseYI C'CCAG_C
Cuts at: 0 593 1346 1488
Size: 593 753 142
Fragments arranged by size:
753 593 142
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 364 1224 1488
Size: 364 860 264
Fragments arranged by size:
860 364 264
BsmI GAATG_Cn'
Cuts at: 0 106 1488
Size: 106 1382
BsmBI CGTCTCn'nnnn_
Cuts at: 0 342 894 1488
Size: 342 552 594
Fragments arranged by size:
594 552 342
Bsp1286I G_dGCh'C
Cuts at: 0 82 503 602 773 791 1291 1488
Size: 82 421 99 171 18 500 197
Fragments arranged by size:
500 421 197 171 99 82 18
BspEI T'CCGG_A
Cuts at: 0 818 1488
Size: 818 670
BspHI T'CATG_A
Cuts at: 0 161 740 1488
Size: 161 579 748
Fragments arranged by size:
748 579 161
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 507 532 704 1488
Size: 507 25 172 784
Fragments arranged by size:
784 507 172 25
BsrBI CCG'CTC
Cuts at: 0 315 1488
Size: 315 1173
BsrDI GCAATG_nn'
Cuts at: 0 546 1112 1488
Size: 546 566 376
Fragments arranged by size:
566 546 376
BsrFI r'CCGG_y
Cuts at: 0 587 1291 1488
Size: 587 704 197
Fragments arranged by size:
704 587 197
BsrGI T'GTAC_A
Cuts at: 0 56 1010 1488
Size: 56 954 478
Fragments arranged by size:
954 478 56
BssHII G'CGCG_C
Cuts at: 0 1027 1488
Size: 1027 461
BstAPI GCAn_nnn'nTGC
Cuts at: 0 867 1488
Size: 867 621
BstEII G'GTnAC_C
Cuts at: 0 519 1488
Size: 519 969
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1212 1488
Size: 1212 276
BstYI r'GATC_y
Cuts at: 0 355 701 1488
Size: 355 346 787
Fragments arranged by size:
787 355 346
BtgI C'CryG_G
Cuts at: 0 197 987 1174 1488
Size: 197 790 187 314
Fragments arranged by size:
790 314 197 187
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 302 1488
Size: 302 1186
BtsI GCAGTG_nn'
Cuts at: 0 113 1329 1488
Size: 113 1216 159
Fragments arranged by size:
1216 159 113
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 165 200 1488
Size: 165 35 1288
Fragments arranged by size:
1288 165 35
EaeI y'GGCC_r
Cuts at: 0 1049 1457 1488
Size: 1049 408 31
Fragments arranged by size:
1049 408 31
EarI CTCTTCn'nnn_
Cuts at: 0 42 223 1081 1153 1488
Size: 42 181 858 72 335
Fragments arranged by size:
858 335 181 72 42
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1384 1488
Size: 1384 104
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1273 1488
Size: 1273 215
EcoNI CCTnn'n_nnAGG
Cuts at: 0 247 514 1488
Size: 247 267 974
Fragments arranged by size:
974 267 247
EcoO109I rG'GnC_Cy
Cuts at: 0 598 599 1277 1385 1488
Size: 598 1 678 108 103
Fragments arranged by size:
678 598 108 103 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1164 1488
Size: 1164 324
FspI TGC'GCA
Cuts at: 0 1129 1488
Size: 1129 359
HaeII r_GCGC'y
Cuts at: 0 533 903 1488
Size: 533 370 585
Fragments arranged by size:
585 533 370
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 250 253 282 285 630 662 1240 1272
Size: 250 3 29 3 345 32 578 32
Cuts at: 1272 1488
Size: 216
Fragments arranged by size:
578 345 250 216 32 32 29 3
3
HincII GTy'rAC
Cuts at: 0 435 1488
Size: 435 1053
MslI CAynn'nnrTG
Cuts at: 0 184 517 745 1006 1117 1213 1488
Size: 184 333 228 261 111 96 275
Fragments arranged by size:
333 275 261 228 184 111 96
MspA1I CmG'CkG
Cuts at: 0 989 1054 1350 1488
Size: 989 65 296 138
Fragments arranged by size:
989 296 138 65
NcoI C'CATG_G
Cuts at: 0 197 1488
Size: 197 1291
NspI r_CATG'y
Cuts at: 0 63 1011 1488
Size: 63 948 477
Fragments arranged by size:
948 477 63
PciI A'CATG_T
Cuts at: 0 59 1007 1488
Size: 59 948 481
Fragments arranged by size:
948 481 59
PflMI CCAn_nnn'nTGG
Cuts at: 0 1251 1488
Size: 1251 237
PfoI T'CCnGG_A
Cuts at: 0 299 923 1488
Size: 299 624 565
Fragments arranged by size:
624 565 299
PpuMI rG'GwC_Cy
Cuts at: 0 1385 1488
Size: 1385 103
PspOMI G'GGCC_C
Cuts at: 0 598 1488
Size: 598 890
PstI C_TGCA'G
Cuts at: 0 207 529 701 1488
Size: 207 322 172 787
Fragments arranged by size:
787 322 207 172
PvuII CAG'CTG
Cuts at: 0 1350 1488
Size: 1350 138
RsrII CG'GwC_CG
Cuts at: 0 658 1488
Size: 658 830
SacII CC_GC'GG
Cuts at: 0 990 1488
Size: 990 498
SfcI C'TryA_G
Cuts at: 0 203 525 697 1488
Size: 203 322 172 791
Fragments arranged by size:
791 322 203 172
SmlI C'TyrA_G
Cuts at: 0 244 1488
Size: 244 1244
StyI C'CwwG_G
Cuts at: 0 101 197 1488
Size: 101 96 1291
Fragments arranged by size:
1291 101 96
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1167 1258 1360 1398 1488
Size: 1167 91 102 38 90
Fragments arranged by size:
1167 102 91 90 38
TatI w'GTAC_w
Cuts at: 0 56 1010 1488
Size: 56 954 478
Fragments arranged by size:
954 478 56
Tth111I GACn'n_nGTC
Cuts at: 0 1189 1488
Size: 1189 299
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1209 1354 1488
Size: 1209 145 134
Fragments arranged by size:
1209 145 134
ZraI GAC'GTC
Cuts at: 0 678 1038 1488
Size: 678 360 450
Fragments arranged by size:
678 450 360
Enzymes that do cut:
AarI AatII AccI AflIII AgeI AhdI AleI AlwNI
ApaI AvaI BanI BanII BbsI BbvCI Bme1580I BmgBI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BstAPI
BstEII BstXI BstYI BtgI BtgZI BtsI CspCI EaeI
EarI EciI Eco57MI EcoNI EcoO109I FalI FspI HaeII
Hin4I HincII MslI MspA1I NcoI NspI PciI PflMI
PfoI PpuMI PspOMI PstI PvuII RsrII SacII SfcI
SmlI StyI TaqII TatI Tth111I XcmI ZraI
Enzymes that do not cut:
Acc65I AclI AcuI AfeI AflII AlfI AloI ApaLI
ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI
BcgI BciVI BclI BfrBI BglI BglII BlpI BmrI
BmtI BplI BsiEI BsiHKAI BsiWI BssSI BstBI BstZ17I
Bsu36I ClaI DraI DraIII DrdI EagI EcoICRI EcoRI
EcoRV FseI FspAI HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PmeI PmlI PpiI
PshAI PsiI PspXI PsrI PvuI SacI SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI
TstI XbaI XhoI XmaI XmnI