(Circular) (Six-base) MAPSORT of: pFN21AB7651.seq Check: 3995 from: 1 to: 5695 pFN21AB7651 5695 bp With 182 enzymes: SgfI * October 20, 2009 10:56 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3505 278 Size: 53 83 186 936 1969 2468 Fragments arranged by size: 2468 1969 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3038 1937 Size: 1101 4594 Acc65I G'GTAC_C Cuts at: 3016 3016 Size: 5695 AclI AA'CG_TT Cuts at: 1563 3822 4195 1563 Size: 2259 373 3063 Fragments arranged by size: 3063 2259 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3758 5142 5410 1632 Size: 2126 1384 268 1917 Fragments arranged by size: 2126 1917 1384 268 AfeI AGC'GCT Cuts at: 2386 2660 2386 Size: 274 5421 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5498 AflIII A'CryG_T Cuts at: 1203 4594 5571 1203 Size: 3391 977 1327 Fragments arranged by size: 3391 1327 977 AgeI A'CCGG_T Cuts at: 4526 4526 Size: 5695 AhdI GACnn_n'nnGTC Cuts at: 4424 4424 Size: 5695 AleI CACnn'nnGTG Cuts at: 1166 2975 1166 Size: 1809 3886 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2846 1184 Size: 1662 4033 AlwNI CAG_nnn'CTG Cuts at: 2452 2875 2929 3438 5010 2452 Size: 423 54 509 1572 3137 Fragments arranged by size: 3137 1572 509 423 54 ApaLI G'TGCA_C Cuts at: 2897 3752 4908 2897 Size: 855 1156 3684 Fragments arranged by size: 3684 1156 855 ApoI r'AATT_y Cuts at: 1493 3004 3230 3382 5491 1493 Size: 1511 226 152 2109 1697 Fragments arranged by size: 2109 1697 1511 226 152 AseI AT'TA_AT Cuts at: 160 1032 4249 160 Size: 872 3217 1606 Fragments arranged by size: 3217 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5695 AvaI C'yCGr_G Cuts at: 1870 1943 2035 3020 1870 Size: 73 92 985 4545 Fragments arranged by size: 4545 985 92 73 BamHI G'GATC_C Cuts at: 3025 3392 3025 Size: 367 5328 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2162 2273 2369 2404 3016 Size: 325 217 627 375 111 96 35 612 Cuts at: 3016 4471 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 612 375 325 217 111 96 35 BanII G_rGCy'C Cuts at: 1391 2052 2189 3014 1391 Size: 661 137 825 4072 Fragments arranged by size: 4072 825 661 137 BbeI G_GCGC'C Cuts at: 2166 2277 2166 Size: 111 5584 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4724 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3886 3920 1903 Size: 34 1949 34 3678 Fragments arranged by size: 3678 1949 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3587 4797 3587 Size: 1210 4485 BclI T'GATC_A Cuts at: 1548 1548 Size: 5695 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2253 2262 2705 Size: 107 122 71 631 605 581 9 443 Cuts at: 2705 4306 136 Size: 1601 1525 Fragments arranged by size: 1601 1525 631 605 581 443 122 107 71 9 BglII A'GATC_T Cuts at: 2640 5690 2640 Size: 3050 2645 BlpI GC'TnA_GC Cuts at: 3112 3112 Size: 5695 Bme1580I G_kGCm'C Cuts at: 1790 2380 2478 2901 3756 4912 1790 Size: 590 98 423 855 1156 2573 Fragments arranged by size: 2573 1156 855 590 423 98 BmgBI CAC'GTC Cuts at: 1318 2622 2973 1318 Size: 1304 351 4040 Fragments arranged by size: 4040 1304 351 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3426 4384 442 Size: 416 53 83 399 24 2009 958 1753 Fragments arranged by size: 2009 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5695 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2108 2229 2253 2672 4355 1239 Size: 869 121 24 419 1683 2579 Fragments arranged by size: 2579 1683 869 419 121 24 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3820 4685 4983 3820 Size: 865 298 4532 Fragments arranged by size: 4532 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4358 882 Size: 617 2859 2219 Fragments arranged by size: 2859 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3486 493 Size: 711 2282 2702 Fragments arranged by size: 2702 2282 711 BsaBI GATnn'nnATC Cuts at: 5689 5689 Size: 5695 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2163 2274 2499 2594 Size: 53 83 186 936 630 111 225 95 Cuts at: 2594 2981 3502 3884 4519 275 Size: 387 521 382 635 1451 Fragments arranged by size: 1451 936 635 630 521 387 382 225 186 111 95 83 53 BsaWI w'CCGG_w Cuts at: 2589 4128 4526 4800 4947 5358 2589 Size: 1539 398 274 147 411 2926 Fragments arranged by size: 2926 1539 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5470 Fragments arranged by size: 5470 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2226 1186 Size: 1040 4655 BseYI C'CCAG_C Cuts at: 2445 4898 5394 2445 Size: 2453 496 2746 Fragments arranged by size: 2746 2453 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5122 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2196 2691 3145 3906 4934 1540 Size: 275 186 195 495 454 761 1028 2301 Fragments arranged by size: 2301 1028 761 495 454 275 195 186 BsiHKAI G_wGCw'C Cuts at: 2013 2901 3756 3841 4912 2013 Size: 888 855 85 1071 2796 Fragments arranged by size: 2796 1071 888 855 85 BsmI GAATG_Cn' Cuts at: 3206 3299 3206 Size: 93 5602 BsmBI CGTCTCn'nnnn_ Cuts at: 2767 2767 Size: 5695 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2013 2052 2189 2380 2478 2901 3014 Size: 399 223 39 137 191 98 423 113 Cuts at: 3014 3756 3841 4912 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 423 399 223 191 137 113 98 85 39 BspEI T'CCGG_A Cuts at: 5358 5358 Size: 5695 BspHI T'CATG_A Cuts at: 3584 5314 3584 Size: 1730 3965 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2725 3050 844 Size: 1881 325 3489 Fragments arranged by size: 3489 1881 325 BsrBI CCG'CTC Cuts at: 1394 1823 2216 2422 3582 1394 Size: 429 393 206 1160 3507 Fragments arranged by size: 3507 1160 429 393 206 BsrDI GCAATG_nn' Cuts at: 65 1248 4190 4364 65 Size: 1183 2942 174 1396 Fragments arranged by size: 2942 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2297 2407 2459 2757 4339 4526 1700 Size: 110 487 110 52 298 1582 187 2869 Fragments arranged by size: 2869 1582 487 298 187 110 110 52 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5695 BssHII G'CGCG_C Cuts at: 1925 2695 1925 Size: 770 4925 BssSI C'ACGA_G Cuts at: 3755 4767 3755 Size: 1012 4683 BstAPI GCAn_nnn'nTGC Cuts at: 794 2929 794 Size: 2135 3560 BstBI TT'CG_AA Cuts at: 4499 4499 Size: 5695 BstEII G'GTnAC_C Cuts at: 1184 2573 1184 Size: 1389 4306 BstYI r'GATC_y Cuts at: 1972 2073 2640 3025 3392 3777 3794 5246 5332 Size: 101 567 385 367 385 17 1452 86 Cuts at: 5332 5344 5690 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 567 385 385 367 346 101 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2190 2433 2936 513 Size: 552 939 186 243 503 3272 Fragments arranged by size: 3272 939 552 503 243 186 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4956 Fragments arranged by size: 4956 683 56 BtsI GCAGTG_nn' Cuts at: 3211 4005 4025 3211 Size: 794 20 4881 Fragments arranged by size: 4881 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5695 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5660 DraI TTT'AAA Cuts at: 2999 2999 Size: 5695 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4702 809 Size: 507 3386 1802 Fragments arranged by size: 3386 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2193 2454 2688 Size: 54 1425 50 275 18 363 261 234 Cuts at: 2688 2706 3142 4031 5660 8 Size: 18 436 889 1629 43 Fragments arranged by size: 1629 1425 889 436 363 275 261 234 54 50 43 18 18 EagI C'GGCC_G Cuts at: 1537 1812 2193 2688 3142 1537 Size: 275 381 495 454 4090 Fragments arranged by size: 4090 495 454 381 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2852 2918 3625 1353 Size: 1499 66 707 3423 Fragments arranged by size: 3423 1499 707 66 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2776 4282 4656 4802 387 Size: 1064 138 1187 1506 374 146 1280 Fragments arranged by size: 1506 1280 1187 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2108 2229 2253 2672 3758 4355 5142 Size: 393 476 121 24 419 1086 597 787 Cuts at: 5142 5410 1239 Size: 268 1524 Fragments arranged by size: 1524 1086 787 597 476 419 393 268 121 24 EcoNI CCTnn'n_nnAGG Cuts at: 2708 2708 Size: 5695 EcoO109I rG'GnC_Cy Cuts at: 2108 2220 2237 2288 2108 Size: 112 17 51 5515 Fragments arranged by size: 5515 112 51 17 EcoRI G'AATT_C Cuts at: 3004 3382 3004 Size: 378 5317 FspI TGC'GCA Cuts at: 2733 4201 2733 Size: 1468 4227 HaeII r_GCGC'y Cuts at: 1678 2166 2277 2388 2662 2717 4842 1678 Size: 488 111 111 274 55 2125 2531 Fragments arranged by size: 2531 2125 488 274 111 111 55 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4336 4368 4410 4442 1373 Size: 32 135 32 2764 32 42 32 2626 Fragments arranged by size: 2764 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2503 3039 3285 669 Size: 988 281 565 536 246 3079 Fragments arranged by size: 3079 988 565 536 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5695 HpaI GTT'AAC Cuts at: 3285 3285 Size: 5695 KasI G'GCGC_C Cuts at: 2162 2273 2162 Size: 111 5584 KpnI G_GTAC'C Cuts at: 3020 3020 Size: 5695 MfeI C'AATT_G Cuts at: 3294 3294 Size: 5695 MluI A'CGCG_T Cuts at: 5571 5571 Size: 5695 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2137 4809 4993 1305 Size: 832 2672 184 2007 Fragments arranged by size: 2672 2007 832 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2456 2708 10 Size: 54 1425 343 624 252 2997 Fragments arranged by size: 2997 1425 624 343 252 54 MslI CAynn'nnrTG Cuts at: 518 1166 2975 3653 4012 4171 518 Size: 648 1809 678 359 159 2042 Fragments arranged by size: 2042 1809 678 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2192 2364 2875 2882 3111 3788 4936 5181 Size: 586 172 511 7 229 677 1148 245 Cuts at: 5181 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 586 511 245 229 172 7 NaeI GCC'GGC Cuts at: 2409 2409 Size: 5695 NarI GG'CG_CC Cuts at: 2163 2274 2163 Size: 111 5584 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4204 Fragments arranged by size: 4204 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5695 NgoMIV G'CCGG_C Cuts at: 2407 2407 Size: 5695 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5695 NotI GC'GGCC_GC Cuts at: 2193 3142 2193 Size: 949 4746 NspI r_CATG'y Cuts at: 1132 1229 3053 4598 1132 Size: 97 1824 1545 2229 Fragments arranged by size: 2229 1824 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5695 PciI A'CATG_T Cuts at: 4594 4594 Size: 5695 PflMI CCAn_nnn'nTGG Cuts at: 1108 2452 1108 Size: 1344 4351 PfoI T'CCnGG_A Cuts at: 2124 2124 Size: 5695 PmeI GTTT'AAAC Cuts at: 2999 2999 Size: 5695 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3764 3796 5277 5309 3764 Size: 32 1481 32 4150 Fragments arranged by size: 4150 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2108 2237 2288 2108 Size: 129 51 5515 Fragments arranged by size: 5515 129 51 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5695 PsiI TTA'TAA Cuts at: 3265 3265 Size: 5695 PstI C_TGCA'G Cuts at: 830 2282 2640 2835 2925 2934 3047 830 Size: 1452 358 195 90 9 113 3478 Fragments arranged by size: 3478 1452 358 195 113 90 9 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5695 SacII CC_GC'GG Cuts at: 2193 2193 Size: 5695 SalI G'TCGA_C Cuts at: 1936 3037 1936 Size: 1101 4594 SanDI GG'GwC_CC Cuts at: 2108 2108 Size: 5695 SapI GCTCTTCn'nnn_ Cuts at: 2852 2918 2852 Size: 66 5629 SbfI CC_TGCA'GG Cuts at: 3047 3047 Size: 5695 ScaI AGT'ACT Cuts at: 3943 5655 3943 Size: 1712 3983 SexAI A'CCwGG_T Cuts at: 2744 2744 Size: 5695 SfcI C'TryA_G Cuts at: 826 1045 2278 2636 2831 2921 2930 3043 4178 Size: 219 1233 358 195 90 9 113 1135 Cuts at: 4178 4859 5050 826 Size: 681 191 1471 Fragments arranged by size: 1471 1233 1135 681 358 219 195 191 113 90 9 SfiI GGCCn_nnn'nGGCC Cuts at: 2253 2253 Size: 5695 SfoI GGC'GCC Cuts at: 2164 2275 2164 Size: 111 5584 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5695 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5695 SmaI CCC'GGG Cuts at: 1872 2037 3022 1872 Size: 165 985 4545 Fragments arranged by size: 4545 985 165 SmlI C'TyrA_G Cuts at: 820 1017 1943 3799 4700 4962 820 Size: 197 926 1856 901 262 1553 Fragments arranged by size: 1856 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3486 493 Size: 2993 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5695 SphI G_CATG'C Cuts at: 1132 3053 1132 Size: 1921 3774 SspI AAT'ATT Cuts at: 5 52 3619 5 Size: 47 3567 2081 Fragments arranged by size: 3567 2081 47 StuI AGG'CCT Cuts at: 1517 2849 1517 Size: 1332 4363 StyI C'CwwG_G Cuts at: 513 1065 2004 2143 2322 2709 3134 513 Size: 552 939 139 179 387 425 3074 Fragments arranged by size: 3074 939 552 425 387 179 139 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2741 3722 3739 3892 4077 4522 325 Size: 1255 1161 981 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1161 981 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3941 5653 96 Size: 274 80 33 51 1443 1964 1712 138 Fragments arranged by size: 1964 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5655 5687 5655 Size: 32 5663 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5695 XbaI T'CTAG_A Cuts at: 3031 3031 Size: 5695 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2011 Size: 5695 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5695 XmaI C'CCGG_G Cuts at: 1870 2035 3020 1870 Size: 165 985 4545 Fragments arranged by size: 4545 985 165 XmnI GAAnn'nnTTC Cuts at: 2660 3824 2660 Size: 1164 4531 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3503 276 Size: 53 83 186 936 1969 2468 Fragments arranged by size: 2468 1969 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SrfI SwaI