(Circular) (Six-base) MAPSORT of: pFN21AE0154.seq Check: 3657 from: 1 to: 5599 pFN21AE0154 5599 bp With 182 enzymes: SgfI * October 20, 2009 10:45 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2150 2150 Size: 5599 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3409 278 Size: 53 83 186 936 1873 2468 Fragments arranged by size: 2468 1873 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2942 1937 Size: 1005 4594 Acc65I G'GTAC_C Cuts at: 2070 2920 2070 Size: 850 4749 AclI AA'CG_TT Cuts at: 1563 3726 4099 1563 Size: 2163 373 3063 Fragments arranged by size: 3063 2163 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3662 5046 5314 1632 Size: 2030 1384 268 1917 Fragments arranged by size: 2030 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5402 AflIII A'CryG_T Cuts at: 1203 4498 5475 1203 Size: 3295 977 1327 Fragments arranged by size: 3295 1327 977 AgeI A'CCGG_T Cuts at: 4430 4430 Size: 5599 AhdI GACnn_n'nnGTC Cuts at: 4328 4328 Size: 5599 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5599 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2232 1184 Size: 1048 4551 AlwNI CAG_nnn'CTG Cuts at: 3342 4914 3342 Size: 1572 4027 ApaLI G'TGCA_C Cuts at: 3656 4812 3656 Size: 1156 4443 ApoI r'AATT_y Cuts at: 1493 2185 2672 2908 3134 3286 5395 1493 Size: 692 487 236 226 152 2109 1697 Fragments arranged by size: 2109 1697 692 487 236 226 152 AseI AT'TA_AT Cuts at: 160 1032 4153 160 Size: 872 3121 1606 Fragments arranged by size: 3121 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5599 AvaI C'yCGr_G Cuts at: 1870 1943 2924 1870 Size: 73 981 4545 Fragments arranged by size: 4545 981 73 BamHI G'GATC_C Cuts at: 2929 3296 2929 Size: 367 5232 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2012 2070 2920 4375 618 Size: 325 217 627 225 58 850 1455 1842 Fragments arranged by size: 1842 1455 850 627 325 225 217 58 BanII G_rGCy'C Cuts at: 1391 2918 1391 Size: 1527 4072 BbeI G_GCGC'C Cuts at: 2016 2016 Size: 5599 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2898 928 Size: 971 999 3629 Fragments arranged by size: 3629 999 971 BbvCI CC'TCA_GC Cuts at: 2522 2522 Size: 5599 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3790 3824 1903 Size: 34 1853 34 3678 Fragments arranged by size: 3678 1853 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2469 2502 3491 4701 2469 Size: 33 989 1210 3367 Fragments arranged by size: 3367 1210 989 33 BclI T'GATC_A Cuts at: 1548 1548 Size: 5599 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4210 136 Size: 107 122 71 631 605 2538 1525 Fragments arranged by size: 2538 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5594 5594 Size: 5599 BlpI GC'TnA_GC Cuts at: 3016 3016 Size: 5599 Bme1580I G_kGCm'C Cuts at: 1790 2581 2832 3660 4816 1790 Size: 791 251 828 1156 2573 Fragments arranged by size: 2573 1156 828 791 251 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5599 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2392 3330 4288 Size: 416 53 83 399 24 975 938 958 Cuts at: 4288 442 Size: 1753 Fragments arranged by size: 1753 975 958 938 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5599 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2228 2392 4259 1239 Size: 989 164 1867 2579 Fragments arranged by size: 2579 1867 989 164 Bpu10I CC'TnA_GC Cuts at: 2522 2522 Size: 5599 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3724 4589 4887 3724 Size: 865 298 4436 Fragments arranged by size: 4436 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4262 882 Size: 617 2763 2219 Fragments arranged by size: 2763 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2116 2389 3390 493 Size: 711 912 273 1001 2702 Fragments arranged by size: 2702 1001 912 711 273 BsaBI GATnn'nnATC Cuts at: 5593 5593 Size: 5599 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2013 3406 3788 4423 Size: 53 83 186 936 480 1393 382 635 Cuts at: 4423 275 Size: 1451 Fragments arranged by size: 1451 1393 936 635 480 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4032 4430 4704 4851 5262 4032 Size: 398 274 147 411 4369 Fragments arranged by size: 4369 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2378 2408 1178 Size: 30 165 30 975 30 4369 Fragments arranged by size: 4369 975 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5599 BseYI C'CCAG_C Cuts at: 2157 2495 4802 5298 2157 Size: 338 2307 496 2458 Fragments arranged by size: 2458 2307 496 338 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5026 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2182 3049 3810 4838 1540 Size: 275 186 181 867 761 1028 2301 Fragments arranged by size: 2301 1028 867 761 275 186 181 BsiHKAI G_wGCw'C Cuts at: 2242 3660 3745 4816 2242 Size: 1418 85 1071 3025 Fragments arranged by size: 3025 1418 1071 85 BsmI GAATG_Cn' Cuts at: 3110 3203 3110 Size: 93 5506 BsmBI CGTCTCn'nnnn_ Cuts at: 2059 2059 Size: 5599 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2242 2581 2832 2918 3660 3745 4816 Size: 399 452 339 251 86 742 85 1071 Cuts at: 4816 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 452 399 339 251 86 85 BspEI T'CCGG_A Cuts at: 5262 5262 Size: 5599 BspHI T'CATG_A Cuts at: 2379 3488 5218 2379 Size: 1109 1730 2760 Fragments arranged by size: 2760 1730 1109 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2150 2954 844 Size: 1306 804 3489 Fragments arranged by size: 3489 1306 804 BsrBI CCG'CTC Cuts at: 1394 1823 3486 1394 Size: 429 1663 3507 Fragments arranged by size: 3507 1663 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4094 4268 65 Size: 1183 2846 174 1396 Fragments arranged by size: 2846 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4243 4430 1700 Size: 110 2433 187 2869 Fragments arranged by size: 2869 2433 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5599 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5599 BssSI C'ACGA_G Cuts at: 3659 4671 3659 Size: 1012 4587 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5599 BstBI TT'CG_AA Cuts at: 4403 4403 Size: 5599 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5599 BstYI r'GATC_y Cuts at: 1972 2929 3296 3681 3698 5150 5236 5248 5594 Size: 957 367 385 17 1452 86 12 346 Cuts at: 5594 1972 Size: 1977 Fragments arranged by size: 1977 1452 957 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2091 513 Size: 552 1026 4021 Fragments arranged by size: 4021 1026 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4860 Fragments arranged by size: 4860 683 56 BtsI GCAGTG_nn' Cuts at: 2152 2808 2836 3115 3909 3929 2152 Size: 656 28 279 794 20 3822 Fragments arranged by size: 3822 794 656 279 28 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5599 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5564 DraI TTT'AAA Cuts at: 2359 2511 2903 2359 Size: 152 392 5055 Fragments arranged by size: 5055 392 152 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2480 4606 809 Size: 507 1164 2126 1802 Fragments arranged by size: 2126 1802 1164 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2043 2179 3046 Size: 54 1425 50 275 18 213 136 867 Cuts at: 3046 3935 5564 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 867 275 213 136 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2179 3046 1537 Size: 275 367 867 4090 Fragments arranged by size: 4090 867 367 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2359 2473 3529 1353 Size: 1006 114 1056 3423 Fragments arranged by size: 3423 1056 1006 114 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4186 4560 4706 387 Size: 1064 138 2597 374 146 1280 Fragments arranged by size: 2597 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2228 2392 3662 4259 5046 5314 1239 Size: 393 596 164 1270 597 787 268 1524 Fragments arranged by size: 1524 1270 787 597 596 393 268 164 EcoRI G'AATT_C Cuts at: 2672 2908 3286 2672 Size: 236 378 4985 Fragments arranged by size: 4985 378 236 EcoRV GAT'ATC Cuts at: 2845 2845 Size: 5599 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2230 2230 Size: 5599 FspI TGC'GCA Cuts at: 2265 4105 2265 Size: 1840 3759 HaeII r_GCGC'y Cuts at: 1678 2016 4746 1678 Size: 338 2730 2531 Fragments arranged by size: 2730 2531 338 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4240 4272 4314 4346 1373 Size: 32 135 32 2668 32 42 32 2626 Fragments arranged by size: 2668 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2943 3189 669 Size: 988 281 1005 246 3079 Fragments arranged by size: 3079 1005 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5599 HpaI GTT'AAC Cuts at: 3189 3189 Size: 5599 KasI G'GCGC_C Cuts at: 2012 2012 Size: 5599 KpnI G_GTAC'C Cuts at: 2074 2924 2074 Size: 850 4749 MfeI C'AATT_G Cuts at: 2350 3198 2350 Size: 848 4751 MluI A'CGCG_T Cuts at: 5475 5475 Size: 5599 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4713 4897 1305 Size: 3408 184 2007 Fragments arranged by size: 3408 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2045 10 Size: 54 1425 343 213 3564 Fragments arranged by size: 3564 1425 343 213 54 MslI CAynn'nnrTG Cuts at: 518 1166 3557 3916 4075 518 Size: 648 2391 359 159 2042 Fragments arranged by size: 2391 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2248 2526 3015 3692 4840 5085 1606 Size: 642 278 489 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 642 489 278 245 NarI GG'CG_CC Cuts at: 2013 2013 Size: 5599 NcoI C'CATG_G Cuts at: 513 1065 2091 513 Size: 552 1026 4021 Fragments arranged by size: 4021 1026 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5599 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5599 NotI GC'GGCC_GC Cuts at: 3046 3046 Size: 5599 NspI r_CATG'y Cuts at: 1132 1229 2957 4502 1132 Size: 97 1728 1545 2229 Fragments arranged by size: 2229 1728 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5599 PciI A'CATG_T Cuts at: 4498 4498 Size: 5599 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5599 PmeI GTTT'AAAC Cuts at: 2903 2903 Size: 5599 PmlI CAC'GTG Cuts at: 2389 2389 Size: 5599 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3668 3700 5181 5213 3668 Size: 32 1481 32 4054 Fragments arranged by size: 4054 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5599 PsiI TTA'TAA Cuts at: 2562 3169 2562 Size: 607 4992 PstI C_TGCA'G Cuts at: 830 2951 830 Size: 2121 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5599 PvuII CAG'CTG Cuts at: 2248 2526 2248 Size: 278 5321 SalI G'TCGA_C Cuts at: 1936 2941 1936 Size: 1005 4594 SapI GCTCTTCn'nnn_ Cuts at: 2359 2359 Size: 5599 SbfI CC_TGCA'GG Cuts at: 2951 2951 Size: 5599 ScaI AGT'ACT Cuts at: 3847 5559 3847 Size: 1712 3887 SfcI C'TryA_G Cuts at: 826 1045 2947 4082 4763 4954 826 Size: 219 1902 1135 681 191 1471 Fragments arranged by size: 1902 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2014 2014 Size: 5599 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5599 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5599 SmaI CCC'GGG Cuts at: 1872 2926 1872 Size: 1054 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3703 4604 4866 820 Size: 197 926 1760 901 262 1553 Fragments arranged by size: 1760 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3390 493 Size: 2897 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5599 SphI G_CATG'C Cuts at: 1132 2957 1132 Size: 1825 3774 SspI AAT'ATT Cuts at: 5 52 3523 5 Size: 47 3471 2081 Fragments arranged by size: 3471 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5599 StyI C'CwwG_G Cuts at: 513 1065 2040 2091 2617 3038 513 Size: 552 975 51 526 421 3074 Fragments arranged by size: 3074 975 552 526 421 51 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2149 3626 3643 3796 3981 4426 325 Size: 1255 569 1477 17 153 185 445 1498 Fragments arranged by size: 1498 1477 1255 569 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2322 2608 3845 Size: 274 80 33 51 1443 345 286 1237 Cuts at: 3845 5557 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1237 345 286 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2378 2410 2469 2501 5559 5591 2378 Size: 32 59 32 3058 32 2386 Fragments arranged by size: 3058 2386 59 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5599 XbaI T'CTAG_A Cuts at: 2884 2935 2884 Size: 51 5548 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5599 XmaI C'CCGG_G Cuts at: 1870 2924 1870 Size: 1054 4545 XmnI GAAnn'nnTTC Cuts at: 3728 3728 Size: 5599 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3407 276 Size: 53 83 186 936 1873 2468 Fragments arranged by size: 2468 1873 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SrfI SwaI XcmI