Restriction map for
pFN21AE0464
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# NO CUT ENZYMES ( 180 enzymes) #
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AatII Acc65I AccII AccIII AcyI
AfaI AflIII AgeI AhdI Alw44I
AlwI AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AvaI AvaII AviII
AvrII BalI BamHI BanI BanII
BbeI BbrPI BbvCI BcgI BcnI
BglI BglII BlnI BlpI Bpu1102I
BsaBI BsaHI BsaI BsaJI BsaWI
BseAI BseRI BsgI BsiEI BsiWI
BsmAI BsmBI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrGI BssHII BssSI Bst1107I BstAPI
BstEII BstPI BstUI BstYI BstZ17I
Bsu36I CelII CfoI Cfr13I ClaI
CpoI DdeI DraI DraII DraIII
DrdI DsaI EaeI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoO109I EcoO65I EcoRV EcoT14I EcoT22I
FauI FseI FspI HaeII HaeIII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HpaI KasI KpnI
Ksp632I KspI MaeIII MamI MfeI
MflI MluI MluNI MroI MscI
MslI MspA1I MunI MvnI MwoI
NarI NciI NcoI NdeI NheI
NotI NruI NsiI NspI PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshBI PstI
PvuI PvuII RcaI RsaI RsrII
SacI SacII SalI SapI Sau96I
SbfI ScaI SexAI SfaNI SfiI
SfoI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI StuI StyI
SwaI Tsp45I Tth111I Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccI 503 GTCTAC GT^[AC][GT]AC
AciI 17 CCGC CCGC(-3/-1)
AclI 530 AACGTT AA^CGTT
AflII 451 CTTAAG C^TTAAG
Asp700I 298 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 221 GTGCTC G[AT]GC[AT]^C
BciVI 489 GGATAC GTATCC(6/5)
BclI 584 TGATCA T^GATCA
BfaI 484 CTAG C^TAG
BfrI 451 CTTAAG C^TTAAG
BmyI 221 GTGCTC G[AGT]GC[ACT]^C
BpmI 245 CTGGAG CTGGAG(16/14)
BsaAI 76 CACGTA [CT]AC^GT[GA]
BsiHKAI 221 GTGCTC G[AT]GC[AT]^C
BsmFI 626 GGGAC GGGAC(10/14)
BsmI 141 GAATGC GAATGC(1/-1)
Bsp1286I 221 GTGCTC G[AGT]GC[ACT]^C
BsrDI 121 GCAATG GCAATG(2/0)
BsrFI 148 GCCGGC [GA]^CCGG[CT]
BssKI 244 CCTGG ^CC[ACGT]GG
BstBI 51 TTCGAA TT^CGAA
BstNI 244 CCTGG CC^[AT]GG
BstXI 360 CCATCTTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 148 GCCGGC [GA]^CCGG[CT]
EcoNI 163 CCTAAAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 391 GAATTC G^AATTC
EcoRII 244 CCTGG ^CC[AT]GG
FbaI 584 TGATCA T^GATCA
HapII 149 CCGG C^CGG
HpaII 149 CCGG C^CGG
MaeI 484 CTAG C^TAG
MspI 149 CCGG C^CGG
MvaI 244 CCTGG CC^[AT]GG
NaeI 148 GCCGGC GCC^GGC
NgoMIV 148 GCCGGC G^CCGGC
NlaIV 159 GGAGCC GG[ACGT]^[ACGT]CC
NspV 51 TTCGAA TT^CGAA
PleI 248 GAGTC GAGTC(4/5)
PshAI 628 GACTGTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 530 AACGTT AA^CGTT
ScrFI 244 CCTGG CC^[ACGT]GG
SfuI 51 TTCGAA TT^CGAA
SmlI 451 CTTAAG C^T[CT][GA]AG
SspI 229 AATATT AAT^ATT
TfiI 268 GATTC G^A[AT]TC
TspRI 426 CAGTG CA[GC]TG[ACGT][ACGT]^
XmnI 298 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AcsI 48 AAATTC [GA]^AATT[CT]
391 GAATTC
AluI 421 AGCT AG^CT
482 AGCT
ApoI 48 AAATTC [GA]^AATT[CT]
391 GAATTC
BbsI 201 GAAGAC GAAGAC(2/6)
552 GAAGAC
BpuAI 201 GAAGAC GAAGAC(2/6)
552 GAAGAC
BsiYI 149 CCGGCATTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
163 CCTAAAAAAGG
BslI 149 CCGGCATTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
163 CCTAAAAAAGG
BsrI 609 ACTGG ACTGG(1/-1)
402 CCAGT
BstF5I 25 GGATG GGATG(2/0)
573 GGATG
FokI 25 GGATG GGATG(9/13)
573 GGATG
HinfI 248 GAGTC G^A[ACGT]TC
268 GATTC
MaeII 77 ACGT A^CGT
531 ACGT
NlaIII 380 CATG CATG^
469 CATG
SfcI 283 CTACAG C^T[GA][CT]AG
423 CTACAG
TaqI 52 TCGA T^CGA
187 TCGA
TthHB8I 52 TCGA T^CGA
187 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 15 enzymes) #
#########################################
BbvI Cac8I DpnII Fnu4HI HphI
ItaI MboI MboII MnlI MseI
NdeII Sau3AI Tru9I TseI Tsp509I