Restriction map for
pFN21AE0382
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# NO CUT ENZYMES ( 96 enzymes) #
##################################
AclI AflII AflIII AgeI AhdI
AscI AseI AsnI AspEI AviII
AvrII BbeI BbrPI BcgI BfaI
BfrI BglI BlnI BsaBI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspLU11I
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
Cfr10I CpoI DraI EagI Eam1105I
EclXI Eco52I EcoO65I EcoRI EcoT22I
FseI FspI HindIII HpaI KasI
MaeI MamI MfeI MluI MunI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Van91I XbaI
XhoI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AatII 445 GACGTC GACGT^C
Acc65I 1621 GGTACC G^GTACC
AccIII 1037 TCCGGA T^CCGGA
AcsI 2006 AAATTC [GA]^AATT[CT]
AocI 1357 CCTGAGG CC^T[ACGT]AGG
Aor51HI 268 AGCGCT AGC^GCT
ApoI 2006 AAATTC [GA]^AATT[CT]
Asp700I 1629 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1621 GGTACC G^GTACC
AspI 442 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1351 GGATCC G^GATCC
BbvCI 1852 GCTGAGG CCTCAGC(-5/-2)
BclI 686 TGATCA T^GATCA
BlpI 132 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 132 GCTGAGC GC^T[ACGT]AGC
BsaAI 948 TACGTA [CT]AC^GT[GA]
BsaI 187 GAGACC GGTCTC(1/5)
BseAI 1037 TCCGGA T^CCGGA
BspDI 943 ATCGAT AT^CGAT
BspEI 1037 TCCGGA T^CCGGA
BsrBI 1690 CCGCTC CCGCTC(-3/-3)
BstXI 1571 CCATCGGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1357 CCTGAGG CC^T[ACGT]AGG
CelII 132 GCTGAGC GC^T[ACGT]AGC
ClaI 943 ATCGAT AT^CGAT
DsaI 108 CCGCGG C^C[GA][CT]GG
Eco47III 268 AGCGCT AGC^GCT
Eco81I 1357 CCTGAGG CC^T[ACGT]AGG
EcoNI 1634 CCTTCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 1804 GATATC GAT^ATC
FbaI 686 TGATCA T^GATCA
HgaI 86 GCGTC GACGC(5/10)
KpnI 1621 GGTACC GGTAC^C
KspI 108 CCGCGG CCGC^GG
MroI 1037 TCCGGA T^CCGGA
PflFI 442 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
SacI 45 GAGCTC GAGCT^C
SacII 108 CCGCGG CCGC^GG
SalI 34 GTCGAC G^TCGAC
SmlI 25 CTTGAG C^T[CT][GA]AG
SnaBI 948 TACGTA TAC^GTA
TfiI 524 GATTC G^A[AT]TC
Tth111I 442 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 1629 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccI 34 GTCGAC GT^[AC][GT]AC
448 GTCTAC
AcyI 85 GGCGTC G[GA]^CG[CT]C
445 GACGTC
Alw44I 858 GTGCAC G^TGCAC
1595 GTGCAC
ApaI 1401 GGGCCC GGGCC^C
1567 GGGCCC
ApaLI 858 GTGCAC G^TGCAC
1595 GTGCAC
BanI 1542 GGTGCC G^G[CT][GA]CC
1621 GGTACC
BbsI 841 GTCTTC GAAGAC(2/6)
1372 GTCTTC
BglII 362 AGATCT A^GATCT
1044 AGATCT
BpuAI 841 GTCTTC GAAGAC(2/6)
1372 GTCTTC
BsaHI 85 GGCGTC G[GA]^CG[CT]C
445 GACGTC
BsaWI 190 ACCGGA [AT]^CCGG[AT]
1037 TCCGGA
BseRI 1693 CTCCTC GAGGAG(10/8)
1360 GAGGAG
BsgI 1920 CTGCAC GTGCAG(16/14)
666 GTGCAG
BspHI 516 TCATGA T^CATGA
1376 TCATGA
BstAPI 1518 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1644 GCACACAGTGC
DrdI 1776 GACAGGGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2101 GACATCTTCGTC
EarI 562 GAAGAG CTCTTC(1/4)
1771 GAAGAG
Hin1I 85 GGCGTC G[GA]^CG[CT]C
445 GACGTC
HincII 34 GTCGAC GT[CT]^[GA]AC
978 GTCAAC
HindII 34 GTCGAC GT[CT]^[GA]AC
978 GTCAAC
Ksp632I 562 GAAGAG CTCTTC(1/4)
1771 GAAGAG
RcaI 516 TCATGA T^CATGA
1376 TCATGA
SmaI 242 CCCGGG CCC^GGG
253 CCCGGG
TaqI 35 TCGA T^CGA
944 TCGA
TthHB8I 35 TCGA T^CGA
944 TCGA
XcmI 1571 CCATCGGACTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1892 CCAACACCTTAATGG
XmaCI 242 CCCGGG C^CCGGG
253 CCCGGG
XmaI 242 CCCGGG C^CCGGG
253 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AspHI AvaI AvaII BalI
BanII BbvI BciVI BcnI BmyI
BpmI BsaJI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DraIII EaeI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI Tru9I
TseI Tsp45I Tsp509I TspRI XhoII