(Circular) (Six-base) MAPSORT of: pFN21AE0382.seq Check: 9813 from: 1 to: 6835 pFN21AE0382 6835 bp With 182 enzymes: SgfI * April 15, 2010 09:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2454 4645 278 Size: 53 83 186 936 918 2191 2468 Fragments arranged by size: 2468 2191 936 918 186 83 53 AccI GT'mk_AC Cuts at: 1937 2040 2454 4178 1937 Size: 103 414 1724 4594 Fragments arranged by size: 4594 1724 414 103 Acc65I G'GTAC_C Cuts at: 3626 4156 3626 Size: 530 6305 AclI AA'CG_TT Cuts at: 1563 4962 5335 1563 Size: 3399 373 3063 Fragments arranged by size: 3399 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2128 4898 6282 6550 1632 Size: 496 2770 1384 268 1917 Fragments arranged by size: 2770 1917 1384 496 268 AfeI AGC'GCT Cuts at: 2275 2275 Size: 6835 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6638 AflIII A'CryG_T Cuts at: 1203 5734 6711 1203 Size: 4531 977 1327 Fragments arranged by size: 4531 1327 977 AgeI A'CCGG_T Cuts at: 5666 5666 Size: 6835 AhdI GACnn_n'nnGTC Cuts at: 5564 5564 Size: 6835 AleI CACnn'nnGTG Cuts at: 1166 2183 3932 1166 Size: 1017 1749 4069 Fragments arranged by size: 4069 1749 1017 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3950 1184 Size: 2766 4069 AlwNI CAG_nnn'CTG Cuts at: 3082 3520 3529 3610 4578 6150 3082 Size: 438 9 81 968 1572 3767 Fragments arranged by size: 3767 1572 968 438 81 9 ApaI G_GGCC'C Cuts at: 3410 3576 3410 Size: 166 6669 ApaLI G'TGCA_C Cuts at: 2863 3600 4892 6048 2863 Size: 737 1292 1156 3650 Fragments arranged by size: 3650 1292 1156 737 ApoI r'AATT_y Cuts at: 1493 4011 4144 4370 4522 6631 1493 Size: 2518 133 226 152 2109 1697 Fragments arranged by size: 2518 2109 1697 226 152 133 AseI AT'TA_AT Cuts at: 160 1032 5389 160 Size: 872 4357 1606 Fragments arranged by size: 4357 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6835 AvaI C'yCGr_G Cuts at: 1870 1943 2226 2247 2258 3503 3567 4160 1870 Size: 73 283 21 11 1245 64 593 4545 Fragments arranged by size: 4545 1245 593 283 73 64 21 11 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3491 3524 3491 Size: 33 6802 BamHI G'GATC_C Cuts at: 3356 4165 4532 3356 Size: 809 367 5659 Fragments arranged by size: 5659 809 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3547 3626 4156 5611 618 Size: 325 217 627 1760 79 530 1455 1842 Fragments arranged by size: 1842 1760 1455 627 530 325 217 79 BanII G_rGCy'C Cuts at: 1391 2054 3410 3576 4132 4154 1391 Size: 663 1356 166 556 22 4072 Fragments arranged by size: 4072 1356 663 556 166 22 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2839 3370 928 Size: 971 940 531 4393 Fragments arranged by size: 4393 971 940 531 BbvCI CC'TCA_GC Cuts at: 3858 3858 Size: 6835 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5026 5060 1903 Size: 34 3089 34 3678 Fragments arranged by size: 3678 3089 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3056 3492 3548 4727 5937 3056 Size: 436 56 1179 1210 3954 Fragments arranged by size: 3954 1210 1179 436 56 BclI T'GATC_A Cuts at: 1548 2691 1548 Size: 1143 5692 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5446 136 Size: 107 122 71 631 605 3774 1525 Fragments arranged by size: 3774 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2367 3049 6830 2367 Size: 682 3781 2372 Fragments arranged by size: 3781 2372 682 BlpI GC'TnA_GC Cuts at: 2138 4252 2138 Size: 2114 4721 Bme1580I G_kGCm'C Cuts at: 1790 2867 3410 3576 3604 4896 6052 1790 Size: 1077 543 166 28 1292 1156 2573 Fragments arranged by size: 2573 1292 1156 1077 543 166 28 BmgBI CAC'GTC Cuts at: 1318 2659 3019 1318 Size: 1341 360 5134 Fragments arranged by size: 5134 1341 360 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2740 4566 5524 Size: 416 53 83 399 24 1323 1826 958 Cuts at: 5524 442 Size: 1753 Fragments arranged by size: 1826 1753 1323 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6835 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2761 2761 Size: 6835 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2038 2290 2786 3464 3664 3839 4040 5495 Size: 799 252 496 678 200 175 201 1455 Cuts at: 5495 1239 Size: 2579 Fragments arranged by size: 2579 1455 799 678 496 252 201 200 175 Bpu10I CC'TnA_GC Cuts at: 2881 3858 2881 Size: 977 5858 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2051 4960 5825 6123 2051 Size: 2909 865 298 2763 Fragments arranged by size: 2909 2763 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2186 5498 882 Size: 617 687 3312 2219 Fragments arranged by size: 3312 2219 687 617 BsaAI yAC'GTr Cuts at: 493 1204 2955 4626 493 Size: 711 1751 1671 2702 Fragments arranged by size: 2702 1751 1671 711 BsaBI GATnn'nnATC Cuts at: 6829 6829 Size: 6835 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2091 2451 4642 5024 Size: 53 83 186 936 558 360 2191 382 Cuts at: 5024 5659 275 Size: 635 1451 Fragments arranged by size: 2191 1451 936 635 558 382 360 186 83 53 BsaWI w'CCGG_w Cuts at: 2195 3042 5268 5666 5940 6087 6498 2195 Size: 847 2226 398 274 147 411 2532 Fragments arranged by size: 2532 2226 847 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3674 3704 3990 4020 1178 Size: 30 165 30 2271 30 286 30 3993 Fragments arranged by size: 3993 2271 286 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3380 3689 1186 Size: 2194 309 4332 Fragments arranged by size: 4332 2194 309 BseYI C'CCAG_C Cuts at: 2218 2404 2646 2841 3096 3403 4124 6038 6534 Size: 186 242 195 255 307 721 1914 496 Cuts at: 6534 2218 Size: 2519 Fragments arranged by size: 2519 1914 721 496 307 255 242 195 186 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2692 3910 1163 Size: 573 956 1218 4088 Fragments arranged by size: 4088 1218 956 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4285 5046 6074 1540 Size: 275 186 2284 761 1028 2301 Fragments arranged by size: 2301 2284 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2054 2073 2867 3604 4896 4981 6052 2054 Size: 19 794 737 1292 85 1071 2837 Fragments arranged by size: 2837 1292 1071 794 737 85 19 BsmI GAATG_Cn' Cuts at: 4346 4439 4346 Size: 93 6742 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2054 2073 2867 3410 3576 3604 4132 Size: 399 264 19 794 543 166 28 528 Cuts at: 4132 4154 4896 4981 6052 1391 Size: 22 742 85 1071 2174 Fragments arranged by size: 2174 1071 794 742 543 528 399 264 166 85 28 22 19 BspEI T'CCGG_A Cuts at: 3042 6498 3042 Size: 3456 3379 BspHI T'CATG_A Cuts at: 2521 3381 4724 6454 2521 Size: 860 1343 1730 2902 Fragments arranged by size: 2902 1730 1343 860 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2052 2466 3067 3528 4190 844 Size: 1208 414 601 461 662 3489 Fragments arranged by size: 3489 1208 662 601 461 414 BsrBI CCG'CTC Cuts at: 1394 1823 3697 4722 1394 Size: 429 1874 1025 3507 Fragments arranged by size: 3507 1874 1025 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5330 5504 65 Size: 1183 4082 174 1396 Fragments arranged by size: 4082 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5479 5666 1700 Size: 110 3669 187 2869 Fragments arranged by size: 3669 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6835 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6835 BssSI C'ACGA_G Cuts at: 4895 5907 4895 Size: 1012 5823 BstAPI GCAn_nnn'nTGC Cuts at: 794 3529 3655 794 Size: 2735 126 3974 Fragments arranged by size: 3974 2735 126 BstBI TT'CG_AA Cuts at: 5639 5639 Size: 6835 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6835 BstXI CCAn_nnnn'nTGG Cuts at: 3583 3583 Size: 6835 BstYI r'GATC_y Cuts at: 1972 2367 2595 3049 3356 3436 4165 4532 4917 Size: 395 228 454 307 80 729 367 385 Cuts at: 4917 4934 6386 6472 6484 6830 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 729 454 395 385 367 346 307 228 86 80 17 12 Bsu36I CC'TnA_GG Cuts at: 3363 3363 Size: 6835 BtgI C'CryG_G Cuts at: 513 1065 2004 2113 513 Size: 552 939 109 5235 Fragments arranged by size: 5235 939 552 109 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3748 487 Size: 683 56 2522 3574 Fragments arranged by size: 3574 2522 683 56 BtsI GCAGTG_nn' Cuts at: 2785 2804 3115 4351 5145 5165 2785 Size: 19 311 1236 794 20 4455 Fragments arranged by size: 4455 1236 794 311 20 19 ClaI AT'CG_AT Cuts at: 1554 2949 1554 Size: 1395 5440 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6800 DraI TTT'AAA Cuts at: 4139 4139 Size: 6835 DraIII CAC_nnn'GTG Cuts at: 2392 2929 3655 2392 Size: 537 726 5572 Fragments arranged by size: 5572 726 537 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3787 4112 5842 809 Size: 507 2471 325 1730 1802 Fragments arranged by size: 2471 1802 1730 507 325 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2116 2344 2631 Size: 54 1425 50 275 18 286 228 287 Cuts at: 2631 2784 3238 3492 3692 3993 4282 5171 6800 Size: 153 454 254 200 301 289 889 1629 Cuts at: 6800 8 Size: 43 Fragments arranged by size: 1629 1425 889 454 301 289 287 286 275 254 228 200 153 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4282 1537 Size: 275 2470 4090 Fragments arranged by size: 4090 2470 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2562 3771 4765 1353 Size: 1209 1209 994 3423 Fragments arranged by size: 3423 1209 1209 994 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5422 5796 5942 387 Size: 1064 138 3833 374 146 1280 Fragments arranged by size: 3833 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2052 2052 Size: 6835 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2038 2128 2290 2786 3464 3664 3839 Size: 393 406 90 162 496 678 200 175 Cuts at: 3839 4040 4898 5495 6282 6550 1239 Size: 201 858 597 787 268 1524 Fragments arranged by size: 1524 858 787 678 597 496 406 393 268 201 200 175 162 90 EcoNI CCTnn'n_nnAGG Cuts at: 3643 3643 Size: 6835 EcoO109I rG'GnC_Cy Cuts at: 2223 3407 3517 3572 3607 3976 2223 Size: 1184 110 55 35 369 5082 Fragments arranged by size: 5082 1184 369 110 55 35 EcoRI G'AATT_C Cuts at: 4144 4522 4144 Size: 378 6457 EcoRV GAT'ATC Cuts at: 3811 3811 Size: 6835 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2333 3438 4126 2333 Size: 1105 688 5042 Fragments arranged by size: 5042 1105 688 FspI TGC'GCA Cuts at: 5341 5341 Size: 6835 HaeII r_GCGC'y Cuts at: 1678 2277 3322 3875 5982 1678 Size: 599 1045 553 2107 2531 Fragments arranged by size: 2531 2107 1045 599 553 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2285 2317 2599 2631 2966 Size: 32 135 32 713 32 282 32 335 Cuts at: 2966 2998 3870 3902 3990 4022 5476 5508 5550 Size: 32 872 32 88 32 1454 32 42 Cuts at: 5550 5582 1373 Size: 32 2626 Fragments arranged by size: 2626 1454 872 713 335 282 135 88 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2041 2985 4179 4425 669 Size: 988 281 103 944 1194 246 3079 Fragments arranged by size: 3079 1194 988 944 281 246 103 HindIII A'AGCT_T Cuts at: 748 748 Size: 6835 HpaI GTT'AAC Cuts at: 4425 4425 Size: 6835 KpnI G_GTAC'C Cuts at: 3630 4160 3630 Size: 530 6305 MfeI C'AATT_G Cuts at: 4434 4434 Size: 6835 MluI A'CGCG_T Cuts at: 6711 6711 Size: 6835 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2322 2915 5949 6133 1305 Size: 1017 593 3034 184 2007 Fragments arranged by size: 3034 2007 1017 593 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2346 2786 3240 3494 10 Size: 54 1425 343 514 440 454 254 3351 Fragments arranged by size: 3351 1425 514 454 440 343 254 54 MslI CAynn'nnrTG Cuts at: 518 1166 2183 2870 3299 3621 3752 3905 3932 Size: 648 1017 687 429 322 131 153 27 Cuts at: 3932 4793 5152 5311 518 Size: 861 359 159 2042 Fragments arranged by size: 2042 1017 861 687 648 429 359 322 159 153 131 27 MspA1I CmG'CkG Cuts at: 1606 2115 2404 2650 3478 3526 3924 4251 4928 Size: 509 289 246 828 48 398 327 677 Cuts at: 4928 6076 6321 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 828 677 509 398 327 289 246 245 48 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5344 Fragments arranged by size: 5344 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6835 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6835 NotI GC'GGCC_GC Cuts at: 4282 4282 Size: 6835 NspI r_CATG'y Cuts at: 1132 1229 4193 5738 1132 Size: 97 2964 1545 2229 Fragments arranged by size: 2964 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6835 PciI A'CATG_T Cuts at: 5734 5734 Size: 6835 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6835 PfoI T'CCnGG_A Cuts at: 2054 3432 3441 2054 Size: 1378 9 5448 Fragments arranged by size: 5448 1378 9 PmeI GTTT'AAAC Cuts at: 4139 4139 Size: 6835 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4904 4936 6417 6449 4904 Size: 32 1481 32 5290 Fragments arranged by size: 5290 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2223 3517 3976 2223 Size: 1294 459 5082 Fragments arranged by size: 5082 1294 459 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6835 PsiI TTA'TAA Cuts at: 4405 4405 Size: 6835 PspOMI G'GGCC_C Cuts at: 3406 3572 3406 Size: 166 6669 PstI C_TGCA'G Cuts at: 830 2049 2481 2724 3483 3525 4187 830 Size: 1219 432 243 759 42 662 3478 Fragments arranged by size: 3478 1219 759 662 432 243 42 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6835 PvuII CAG'CTG Cuts at: 3478 3526 3924 3478 Size: 48 398 6389 Fragments arranged by size: 6389 398 48 SacI G_AGCT'C Cuts at: 2054 2054 Size: 6835 SacII CC_GC'GG Cuts at: 2116 2116 Size: 6835 SalI G'TCGA_C Cuts at: 1936 2039 4177 1936 Size: 103 2138 4594 Fragments arranged by size: 4594 2138 103 SanDI GG'GwC_CC Cuts at: 2223 2223 Size: 6835 SbfI CC_TGCA'GG Cuts at: 4187 4187 Size: 6835 ScaI AGT'ACT Cuts at: 5083 6795 5083 Size: 1712 5123 SfcI C'TryA_G Cuts at: 826 1045 2045 2477 2720 2792 3479 3521 3709 Size: 219 1000 432 243 72 687 42 188 Cuts at: 3709 4183 5318 5999 6190 826 Size: 474 1135 681 191 1471 Fragments arranged by size: 1471 1135 1000 687 681 474 432 243 219 191 188 72 42 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6835 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6835 SmaI CCC'GGG Cuts at: 1872 2249 2260 4162 1872 Size: 377 11 1902 4545 Fragments arranged by size: 4545 1902 377 11 SmlI C'TyrA_G Cuts at: 820 1017 1943 2030 4939 5840 6102 820 Size: 197 926 87 2909 901 262 1553 Fragments arranged by size: 2909 1553 926 901 262 197 87 SnaBI TAC'GTA Cuts at: 493 2955 4626 493 Size: 2462 1671 2702 Fragments arranged by size: 2702 2462 1671 SpeI A'CTAG_T Cuts at: 152 152 Size: 6835 SphI G_CATG'C Cuts at: 1132 4193 1132 Size: 3061 3774 SspI AAT'ATT Cuts at: 5 52 4759 5 Size: 47 4707 2081 Fragments arranged by size: 4707 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6835 StyI C'CwwG_G Cuts at: 513 1065 2004 2380 2432 3229 3979 4274 513 Size: 552 939 376 52 797 750 295 3074 Fragments arranged by size: 3074 939 797 750 552 376 295 52 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2979 3575 4862 4879 5032 5217 5662 Size: 1255 1399 596 1287 17 153 185 445 Cuts at: 5662 325 Size: 1498 Fragments arranged by size: 1498 1399 1287 1255 596 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5081 6793 96 Size: 274 80 33 51 1443 3104 1712 138 Fragments arranged by size: 3104 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2764 2796 3573 3605 3674 3706 6795 6827 2764 Size: 32 777 32 69 32 3089 32 2772 Fragments arranged by size: 3089 2772 777 69 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2450 1316 Size: 1134 5701 XbaI T'CTAG_A Cuts at: 4171 4171 Size: 6835 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 3583 3904 2011 Size: 1572 321 4942 Fragments arranged by size: 4942 1572 321 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6835 XmaI C'CCGG_G Cuts at: 1870 2247 2258 4160 1870 Size: 377 11 1902 4545 Fragments arranged by size: 4545 1902 377 11 XmnI GAAnn'nnTTC Cuts at: 3638 4964 3638 Size: 1326 5509 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2452 4643 276 Size: 53 83 186 936 918 2191 2468 Fragments arranged by size: 2468 2191 936 918 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BbeI BfrBI BsiWI BsmBI BstZ17I FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SapI SexAI SfiI SfoI SrfI SwaI