Restriction map for
pFN21AE0382
(Linear) (Six-base) MAPSORT of: KIEE0382.seq Check: 6917 from: 1 to: 2130
KIEE0382.orf
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AatII G_ACGT'C
Cuts at: 0 449 2130
Size: 449 1681
AccI GT'mk_AC
Cuts at: 0 35 449 2130
Size: 35 414 1681
Fragments arranged by size:
1681 414 35
Acc65I G'GTAC_C
Cuts at: 0 1621 2130
Size: 1621 509
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 123 2130
Size: 123 2007
AfeI AGC'GCT
Cuts at: 0 270 2130
Size: 270 1860
AleI CACnn'nnGTG
Cuts at: 0 178 1927 2130
Size: 178 1749 203
Fragments arranged by size:
1749 203 178
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1945 2130
Size: 1945 185
AlwNI CAG_nnn'CTG
Cuts at: 0 1077 1515 1524 1605 2130
Size: 1077 438 9 81 525
Fragments arranged by size:
1077 525 438 81 9
ApaI G_GGCC'C
Cuts at: 0 1405 1571 2130
Size: 1405 166 559
Fragments arranged by size:
1405 559 166
ApaLI G'TGCA_C
Cuts at: 0 858 1595 2130
Size: 858 737 535
Fragments arranged by size:
858 737 535
ApoI r'AATT_y
Cuts at: 0 2006 2130
Size: 2006 124
AvaI C'yCGr_G
Cuts at: 0 221 242 253 1498 1562 2130
Size: 221 21 11 1245 64 568
Fragments arranged by size:
1245 568 221 64 21 11
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1486 1519 2130
Size: 1486 33 611
Fragments arranged by size:
1486 611 33
BamHI G'GATC_C
Cuts at: 0 1351 2130
Size: 1351 779
BanI G'GyrC_C
Cuts at: 0 1542 1621 2130
Size: 1542 79 509
Fragments arranged by size:
1542 509 79
BanII G_rGCy'C
Cuts at: 0 49 1405 1571 2127 2130
Size: 49 1356 166 556 3
Fragments arranged by size:
1356 556 166 49 3
BbsI GAAGACnn'nnnn_
Cuts at: 0 834 1365 2130
Size: 834 531 765
Fragments arranged by size:
834 765 531
BbvCI CC'TCA_GC
Cuts at: 0 1853 2130
Size: 1853 277
BciVI GTATCCnnnnn_n'
Cuts at: 0 1051 1487 1543 2130
Size: 1051 436 56 587
Fragments arranged by size:
1051 587 436 56
BclI T'GATC_A
Cuts at: 0 686 2130
Size: 686 1444
BglII A'GATC_T
Cuts at: 0 362 1044 2130
Size: 362 682 1086
Fragments arranged by size:
1086 682 362
BlpI GC'TnA_GC
Cuts at: 0 133 2130
Size: 133 1997
Bme1580I G_kGCm'C
Cuts at: 0 862 1405 1571 1599 2130
Size: 862 543 166 28 531
Fragments arranged by size:
862 543 531 166 28
BmgBI CAC'GTC
Cuts at: 0 654 1014 2130
Size: 654 360 1116
Fragments arranged by size:
1116 654 360
BmrI ACTGGGnnnn_n'
Cuts at: 0 735 2130
Size: 735 1395
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 756 2130
Size: 756 1374
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 33 285 781 1459 1659 1834 2035 2130
Size: 33 252 496 678 200 175 201 95
Fragments arranged by size:
678 496 252 201 200 175 95 33
Bpu10I CC'TnA_GC
Cuts at: 0 876 1853 2130
Size: 876 977 277
Fragments arranged by size:
977 876 277
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 46 2130
Size: 46 2084
BsaI GGTCTCn'nnnn_
Cuts at: 0 181 2130
Size: 181 1949
BsaAI yAC'GTr
Cuts at: 0 950 2130
Size: 950 1180
BsaHI Gr'CG_yC
Cuts at: 0 86 446 2130
Size: 86 360 1684
Fragments arranged by size:
1684 360 86
BsaWI w'CCGG_w
Cuts at: 0 190 1037 2130
Size: 190 847 1093
Fragments arranged by size:
1093 847 190
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1669 1699 1985 2015 2130
Size: 1669 30 286 30 115
Fragments arranged by size:
1669 286 115 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1375 1684 2130
Size: 1375 309 446
Fragments arranged by size:
1375 446 309
BseYI C'CCAG_C
Cuts at: 0 213 399 641 836 1091 1398 2119 2130
Size: 213 186 242 195 255 307 721 11
Fragments arranged by size:
721 307 255 242 213 195 186 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 687 1905 2130
Size: 687 1218 225
Fragments arranged by size:
1218 687 225
BsiHKAI G_wGCw'C
Cuts at: 0 49 68 862 1599 2130
Size: 49 19 794 737 531
Fragments arranged by size:
794 737 531 49 19
Bsp1286I G_dGCh'C
Cuts at: 0 49 68 862 1405 1571 1599 2127 2130
Size: 49 19 794 543 166 28 528 3
Fragments arranged by size:
794 543 528 166 49 28 19 3
BspEI T'CCGG_A
Cuts at: 0 1037 2130
Size: 1037 1093
BspHI T'CATG_A
Cuts at: 0 516 1376 2130
Size: 516 860 754
Fragments arranged by size:
860 754 516
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 47 461 1062 1523 2130
Size: 47 414 601 461 607
Fragments arranged by size:
607 601 461 414 47
BsrBI CCG'CTC
Cuts at: 0 1692 2130
Size: 1692 438
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1524 1650 2130
Size: 1524 126 480
Fragments arranged by size:
1524 480 126
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1578 2130
Size: 1578 552
BstYI r'GATC_y
Cuts at: 0 362 590 1044 1351 1431 2130
Size: 362 228 454 307 80 699
Fragments arranged by size:
699 454 362 307 228 80
Bsu36I CC'TnA_GG
Cuts at: 0 1358 2130
Size: 1358 772
BtgI C'CryG_G
Cuts at: 0 108 2130
Size: 108 2022
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1743 2130
Size: 1743 387
BtsI GCAGTG_nn'
Cuts at: 0 780 799 1110 2130
Size: 780 19 311 1020
Fragments arranged by size:
1020 780 311 19
ClaI AT'CG_AT
Cuts at: 0 944 2130
Size: 944 1186
DraIII CAC_nnn'GTG
Cuts at: 0 387 924 1650 2130
Size: 387 537 726 480
Fragments arranged by size:
726 537 480 387
DrdI GACnn_nn'nnGTC
Cuts at: 0 1782 2107 2130
Size: 1782 325 23
Fragments arranged by size:
1782 325 23
EaeI y'GGCC_r
Cuts at: 0 111 339 626 779 1233 1487 1687 1988
Size: 111 228 287 153 454 254 200 301
Cuts at: 1988 2130
Size: 142
Fragments arranged by size:
454 301 287 254 228 200 153 142
111
EarI CTCTTCn'nnn_
Cuts at: 0 557 1766 2130
Size: 557 1209 364
Fragments arranged by size:
1209 557 364
EcoICRI GAG'CTC
Cuts at: 0 47 2130
Size: 47 2083
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 33 123 285 781 1459 1659 1834 2035
Size: 33 90 162 496 678 200 175 201
Cuts at: 2035 2130
Size: 95
Fragments arranged by size:
678 496 201 200 175 162 95 90
33
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1638 2130
Size: 1638 492
EcoO109I rG'GnC_Cy
Cuts at: 0 218 1402 1512 1567 1602 1971 2130
Size: 218 1184 110 55 35 369 159
Fragments arranged by size:
1184 369 218 159 110 55 35
EcoRV GAT'ATC
Cuts at: 0 1806 2130
Size: 1806 324
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 328 1433 2121 2130
Size: 328 1105 688 9
Fragments arranged by size:
1105 688 328 9
HaeII r_GCGC'y
Cuts at: 0 272 1317 1870 2130
Size: 272 1045 553 260
Fragments arranged by size:
1045 553 272 260
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 280 312 594 626 961 993 1865 1897
Size: 280 32 282 32 335 32 872 32
Cuts at: 1897 1985 2017 2130
Size: 88 32 113
Fragments arranged by size:
872 335 282 280 113 88 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 36 980 2130
Size: 36 944 1150
Fragments arranged by size:
1150 944 36
KpnI G_GTAC'C
Cuts at: 0 1625 2130
Size: 1625 505
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 317 910 2130
Size: 317 593 1220
Fragments arranged by size:
1220 593 317
MscI TGG'CCA
Cuts at: 0 341 781 1235 1489 2130
Size: 341 440 454 254 641
Fragments arranged by size:
641 454 440 341 254
MslI CAynn'nnrTG
Cuts at: 0 178 865 1294 1616 1747 1900 1927 2130
Size: 178 687 429 322 131 153 27 203
Fragments arranged by size:
687 429 322 203 178 153 131 27
MspA1I CmG'CkG
Cuts at: 0 110 399 645 1473 1521 1919 2130
Size: 110 289 246 828 48 398 211
Fragments arranged by size:
828 398 289 246 211 110 48
PfoI T'CCnGG_A
Cuts at: 0 49 1427 1436 2130
Size: 49 1378 9 694
Fragments arranged by size:
1378 694 49 9
PpuMI rG'GwC_Cy
Cuts at: 0 218 1512 1971 2130
Size: 218 1294 459 159
Fragments arranged by size:
1294 459 218 159
PspOMI G'GGCC_C
Cuts at: 0 1401 1567 2130
Size: 1401 166 563
Fragments arranged by size:
1401 563 166
PstI C_TGCA'G
Cuts at: 0 44 476 719 1478 1520 2130
Size: 44 432 243 759 42 610
Fragments arranged by size:
759 610 432 243 44 42
PvuII CAG'CTG
Cuts at: 0 1473 1521 1919 2130
Size: 1473 48 398 211
Fragments arranged by size:
1473 398 211 48
SacI G_AGCT'C
Cuts at: 0 49 2130
Size: 49 2081
SacII CC_GC'GG
Cuts at: 0 111 2130
Size: 111 2019
SalI G'TCGA_C
Cuts at: 0 34 2130
Size: 34 2096
SanDI GG'GwC_CC
Cuts at: 0 218 2130
Size: 218 1912
SfcI C'TryA_G
Cuts at: 0 40 472 715 787 1474 1516 1704 2130
Size: 40 432 243 72 687 42 188 426
Fragments arranged by size:
687 432 426 243 188 72 42 40
SmaI CCC'GGG
Cuts at: 0 244 255 2130
Size: 244 11 1875
Fragments arranged by size:
1875 244 11
SmlI C'TyrA_G
Cuts at: 0 25 2130
Size: 25 2105
SnaBI TAC'GTA
Cuts at: 0 950 2130
Size: 950 1180
StyI C'CwwG_G
Cuts at: 0 375 427 1224 1974 2130
Size: 375 52 797 750 156
Fragments arranged by size:
797 750 375 156 52
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 974 1570 2130
Size: 974 596 560
Fragments arranged by size:
974 596 560
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 759 791 1568 1600 1669 1701 2130
Size: 759 32 777 32 69 32 429
Fragments arranged by size:
777 759 429 69 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 445 2130
Size: 445 1685
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1578 1899 2130
Size: 1578 321 231
Fragments arranged by size:
1578 321 231
XmaI C'CCGG_G
Cuts at: 0 242 253 2130
Size: 242 11 1877
Fragments arranged by size:
1877 242 11
XmnI GAAnn'nnTTC
Cuts at: 0 1633 2130
Size: 1633 497
ZraI GAC'GTC
Cuts at: 0 447 2130
Size: 447 1683
Enzymes that do cut:
AatII AccI Acc65I AcuI AfeI AleI AlfI AlwNI
ApaI ApaLI ApoI AvaI BaeI BamHI BanI BanII
BbsI BbvCI BciVI BclI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BstAPI BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI DraIII DrdI EaeI EarI EcoICRI
Eco57MI EcoNI EcoO109I EcoRV FalI HaeII Hin4I HincII
KpnI MmeI MscI MslI MspA1I PfoI PpuMI PspOMI
PstI PvuII SacI SacII SalI SanDI SfcI SmaI
SmlI SnaBI StyI TaqII TstI Tth111I XcmI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AclI AflII AflIII AgeI AhdI AloI AscI
AseI AsiSI AvrII BbeI BcgI BfrBI BglI BmtI
BsaBI BsiEI BsiWI BsmI BsmBI BsrDI BsrFI BsrGI
BssHII BssSI BstBI BstEII BstZ17I CspCI DraI EagI
EciI EcoRI FseI FspI FspAI HindIII HpaI KasI
MfeI MluI NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PasI PciI PflMI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI
RsrII SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SpeI SphI SrfI SspI StuI SwaI TatI
XbaI XhoI