Restriction map for
pFN21AE0195
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# NO CUT ENZYMES ( 142 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AcyI AflII AflIII AgeI AhdI
AlwI AocI Aor51HI ApaI ApoI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BalI BamHI
BbeI BbrPI BbvCI BcgI BcnI
BfrI BglII BlnI BlpI Bpu1102I
BsaAI BsaHI BsaWI BseAI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrGI BssSI Bst1107I BstBI BstEII
BstPI BstXI BstYI BstZ17I Bsu36I
CelII ClaI CpoI DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FseI FspI HaeII HgaI Hin1I
HindIII HpaI KasI KpnI KspI
MfeI MflI MluI MluNI MroI
MscI MunI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI ScaI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI SspBI
SspI SwaI TfiI Tsp509I Tth111I
XbaI XcmI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 34 enzymes) #
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AccI 1187 GTAGAC GT^[AC][GT]AC
AfaI 1194 GTAC GT^AC
Alw44I 1083 GTGCAC G^TGCAC
AlwNI 361 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 1083 GTGCAC G^TGCAC
AscI 1139 GGCGCGCC GG^CGCGCC
BciVI 238 GTATCC GTATCC(6/5)
BclI 1067 TGATCA T^GATCA
BfaI 127 CTAG C^TAG
BglI 92 GCCCCAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 1101 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 727 GAGGAG GAGGAG(10/8)
BsgI 1089 CTGCAC GTGCAG(16/14)
DraI 6 TTTAAA TTT^AAA
DrdI 484 GACGGGCCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 829 GAAGAG CTCTTC(1/4)
FauI 972 CCCGC CCCGC(4/6)
FbaI 1067 TGATCA T^GATCA
HincII 493 GTCAAC GT[CT]^[GA]AC
HindII 493 GTCAAC GT[CT]^[GA]AC
Ksp632I 829 GAAGAG CTCTTC(1/4)
MaeI 127 CTAG C^TAG
MaeII 559 ACGT A^CGT
MamI 1101 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 7 TTAA T^TAA
RsaI 1194 GTAC GT^AC
SbfI 774 CCTGCAGG CCTGCA^GG
SexAI 257 ACCAGGT A^CC[AT]GGT
SfiI 91 GGCCCCAGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 229 CTCAAG C^T[CT][GA]AG
Sse8387I 774 CCTGCAGG CCTGCA^GG
TaqI 326 TCGA T^CGA
Tru9I 7 TTAA T^TAA
TthHB8I 326 TCGA T^CGA
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# TWO CUT ENZYMES ( 14 enzymes) #
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AvaI 385 CCCGAG C^[CT]CG[GA]G
466 CCCGAG
BanII 27 GAGCCC G[GA]GC[CT]^C
460 GAGCCC
BbsI 85 GAAGAC GAAGAC(2/6)
481 GAAGAC
BpuAI 85 GAAGAC GAAGAC(2/6)
481 GAAGAC
BsaI 886 GAGACC GGTCTC(1/5)
686 GGTCTC
BsoBI 385 CCCGAG C^[CT]CG[GA]G
466 CCCGAG
BssHII 300 GCGCGC G^CGCGC
1140 GCGCGC
BstAPI 255 GCACCAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
702 GCAGCTGTTGC
EcoNI 720 CCTTCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1127 CCTGGCAGAGG
EcoT14I 863 CCAAGG C^C[AT][AT]GG
1199 CCAAGG
MslI 1076 CACGCCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1095 CATGCTGATG
PpuMI 1118 GGGTCCT [GA]G^G[AT]CC[CT]
1172 GGGTCCC
StuI 123 AGGCCT AGG^CCT
278 AGGCCT
StyI 863 CCAAGG C^C[AT][AT]GG
1199 CCAAGG
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# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AccII AciI AluI AspHI AvaII
BanI BbvI BmyI BpmI BsaJI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrFI BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DraIII EcoO109I EcoRII Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeIII MboI
MboII MnlI MspA1I MspI MvaI
MvnI MwoI NdeII NlaIII NlaIV
PflMI PleI PstI PvuII Sau3AI
Sau96I ScrFI SfaNI SfcI TseI
Tsp45I TspRI Van91I