(Circular) (Six-base) MAPSORT of: pFN21AE0195.seq Check: 1260 from: 1 to: 5935 pFN21AE0195 5935 bp With 182 enzymes: SgfI * October 20, 2009 10:45 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3101 3101 Size: 5935 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3745 278 Size: 53 83 186 936 2209 2468 Fragments arranged by size: 2468 2209 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3193 3278 1937 Size: 1256 85 4594 Fragments arranged by size: 4594 1256 85 Acc65I G'GTAC_C Cuts at: 3256 3256 Size: 5935 AclI AA'CG_TT Cuts at: 1563 4062 4435 1563 Size: 2499 373 3063 Fragments arranged by size: 3063 2499 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2711 3998 5382 5650 1632 Size: 1079 1287 1384 268 1917 Fragments arranged by size: 1917 1384 1287 1079 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5738 AflIII A'CryG_T Cuts at: 1203 4834 5811 1203 Size: 3631 977 1327 Fragments arranged by size: 3631 1327 977 AgeI A'CCGG_T Cuts at: 4766 4766 Size: 5935 AhdI GACnn_n'nnGTC Cuts at: 4664 4664 Size: 5935 AleI CACnn'nnGTG Cuts at: 1166 3085 1166 Size: 1919 4016 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5935 AlwNI CAG_nnn'CTG Cuts at: 2371 3678 5250 2371 Size: 1307 1572 3056 Fragments arranged by size: 3056 1572 1307 ApaLI G'TGCA_C Cuts at: 3088 3992 5148 3088 Size: 904 1156 3875 Fragments arranged by size: 3875 1156 904 ApoI r'AATT_y Cuts at: 1493 3244 3470 3622 5731 1493 Size: 1751 226 152 2109 1697 Fragments arranged by size: 2109 1751 1697 226 152 AscI GG'CGCG_CC Cuts at: 3145 3145 Size: 5935 AseI AT'TA_AT Cuts at: 160 1032 4489 160 Size: 872 3457 1606 Fragments arranged by size: 3457 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5935 AvaI C'yCGr_G Cuts at: 1870 1943 2390 2471 3260 1870 Size: 73 447 81 789 4545 Fragments arranged by size: 4545 789 447 81 73 BamHI G'GATC_C Cuts at: 3265 3632 3265 Size: 367 5568 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2160 2259 2266 3256 4711 Size: 325 217 627 373 99 7 990 1455 Cuts at: 4711 618 Size: 1842 Fragments arranged by size: 1842 1455 990 627 373 325 217 99 7 BanII G_rGCy'C Cuts at: 1391 2036 2469 3254 1391 Size: 645 433 785 4072 Fragments arranged by size: 4072 785 645 433 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2097 2493 928 Size: 971 198 396 4370 Fragments arranged by size: 4370 971 396 198 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4126 4160 1903 Size: 34 2189 34 3678 Fragments arranged by size: 3678 2189 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2254 3827 5037 2254 Size: 1573 1210 3152 Fragments arranged by size: 3152 1573 1210 BclI T'GATC_A Cuts at: 1548 3072 1548 Size: 1524 4411 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2103 4546 136 Size: 107 122 71 631 605 431 2443 1525 Fragments arranged by size: 2443 1525 631 605 431 122 107 71 BglII A'GATC_T Cuts at: 5930 5930 Size: 5935 BlpI GC'TnA_GC Cuts at: 3352 3352 Size: 5935 Bme1580I G_kGCm'C Cuts at: 1790 3092 3996 5152 1790 Size: 1302 904 1156 2573 Fragments arranged by size: 2573 1302 1156 904 BmgBI CAC'GTC Cuts at: 1318 2565 1318 Size: 1247 4688 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2094 2436 3666 Size: 416 53 83 399 24 677 342 1230 Cuts at: 3666 4624 442 Size: 958 1753 Fragments arranged by size: 1753 1230 958 677 416 399 342 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5935 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2348 2393 2645 2738 2939 2966 3169 3204 Size: 1109 45 252 93 201 27 203 35 Cuts at: 3204 4595 1239 Size: 1391 2579 Fragments arranged by size: 2579 1391 1109 252 203 201 93 45 35 27 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2219 4060 4925 5223 2219 Size: 1841 865 298 2931 Fragments arranged by size: 2931 1841 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2697 2885 4598 882 Size: 617 1198 188 1713 2219 Fragments arranged by size: 2219 1713 1198 617 188 BsaAI yAC'GTr Cuts at: 493 1204 3726 493 Size: 711 2522 2702 Fragments arranged by size: 2702 2522 711 BsaBI GATnn'nnATC Cuts at: 3110 5929 3110 Size: 2819 3116 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3742 4124 4759 275 Size: 53 83 186 936 2209 382 635 1451 Fragments arranged by size: 2209 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4368 4766 5040 5187 5598 4368 Size: 398 274 147 411 4705 Fragments arranged by size: 4705 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2723 2753 1178 Size: 30 165 30 1320 30 4360 Fragments arranged by size: 4360 1320 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2747 1186 Size: 1561 4374 BseYI C'CCAG_C Cuts at: 2036 2422 2589 5138 5634 2036 Size: 386 167 2549 496 2337 Fragments arranged by size: 2549 2337 496 386 167 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3079 1163 Size: 573 1343 4019 Fragments arranged by size: 4019 1343 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3385 4146 5174 1540 Size: 275 186 1384 761 1028 2301 Fragments arranged by size: 2301 1384 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2337 2961 3092 3996 4081 5152 2337 Size: 624 131 904 85 1071 3120 Fragments arranged by size: 3120 1071 904 624 131 85 BsmI GAATG_Cn' Cuts at: 3446 3539 3446 Size: 93 5842 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2036 2337 2469 2961 3092 3254 3996 Size: 399 246 301 132 492 131 162 742 Cuts at: 3996 4081 5152 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 492 399 301 246 162 132 131 85 BspEI T'CCGG_A Cuts at: 5598 5598 Size: 5935 BspHI T'CATG_A Cuts at: 3824 5554 3824 Size: 1730 4205 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2511 2610 2712 3101 3290 844 Size: 1667 99 102 389 189 3489 Fragments arranged by size: 3489 1667 389 189 102 99 BsrBI CCG'CTC Cuts at: 1394 1823 3822 1394 Size: 429 1999 3507 Fragments arranged by size: 3507 1999 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4430 4604 65 Size: 1183 3182 174 1396 Fragments arranged by size: 3182 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2070 2256 2494 4579 4766 1700 Size: 110 260 186 238 2085 187 2869 Fragments arranged by size: 2869 2085 260 238 187 186 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5935 BssHII G'CGCG_C Cuts at: 1925 2305 3145 1925 Size: 380 840 4715 Fragments arranged by size: 4715 840 380 BssSI C'ACGA_G Cuts at: 3995 5007 3995 Size: 1012 4923 BstAPI GCAn_nnn'nTGC Cuts at: 794 2266 2713 794 Size: 1472 447 4016 Fragments arranged by size: 4016 1472 447 BstBI TT'CG_AA Cuts at: 4739 4739 Size: 5935 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5935 BstYI r'GATC_y Cuts at: 1972 3265 3632 4017 4034 5486 5572 5584 5930 Size: 1293 367 385 17 1452 86 12 346 Cuts at: 5930 1972 Size: 1977 Fragments arranged by size: 1977 1452 1293 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4444 Fragments arranged by size: 4444 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2305 487 Size: 683 56 1079 4117 Fragments arranged by size: 4117 1079 683 56 BtsI GCAGTG_nn' Cuts at: 3451 4245 4265 3451 Size: 794 20 5121 Fragments arranged by size: 5121 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5935 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5900 DraI TTT'AAA Cuts at: 2013 3239 2013 Size: 1226 4709 DraIII CAC_nnn'GTG Cuts at: 2266 2444 3051 2266 Size: 178 607 5150 Fragments arranged by size: 5150 607 178 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2495 4942 809 Size: 507 1179 2447 1802 Fragments arranged by size: 2447 1802 1179 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3382 4271 5900 Size: 54 1425 50 275 18 1552 889 1629 Cuts at: 5900 8 Size: 43 Fragments arranged by size: 1629 1552 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3382 1537 Size: 275 1570 4090 Fragments arranged by size: 4090 1570 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2829 3865 1353 Size: 1476 1036 3423 Fragments arranged by size: 3423 1476 1036 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2033 4522 4896 5042 387 Size: 1064 138 444 2489 374 146 1280 Fragments arranged by size: 2489 1280 1064 444 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2348 2393 2645 2711 2738 2939 2966 Size: 393 716 45 252 66 27 201 27 Cuts at: 2966 3169 3204 3998 4595 5382 5650 1239 Size: 203 35 794 597 787 268 1524 Fragments arranged by size: 1524 794 787 716 597 393 268 252 203 201 66 45 35 27 27 EcoNI CCTnn'n_nnAGG Cuts at: 2729 3136 2729 Size: 407 5528 EcoO109I rG'GnC_Cy Cuts at: 2452 2520 3124 3178 2452 Size: 68 604 54 5209 Fragments arranged by size: 5209 604 68 54 EcoRI G'AATT_C Cuts at: 3244 3622 3244 Size: 378 5557 FspI TGC'GCA Cuts at: 4441 4441 Size: 5935 HaeII r_GCGC'y Cuts at: 1678 5082 1678 Size: 3404 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4576 4608 4650 4682 1373 Size: 32 135 32 3004 32 42 32 2626 Fragments arranged by size: 3004 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2500 3279 3525 669 Size: 988 281 562 779 246 3079 Fragments arranged by size: 3079 988 779 562 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5935 HpaI GTT'AAC Cuts at: 3525 3525 Size: 5935 KpnI G_GTAC'C Cuts at: 3260 3260 Size: 5935 MfeI C'AATT_G Cuts at: 3534 3534 Size: 5935 MluI A'CGCG_T Cuts at: 5811 5811 Size: 5935 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5049 5233 1305 Size: 3744 184 2007 Fragments arranged by size: 3744 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4113 Fragments arranged by size: 4113 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3085 3104 3893 4252 4411 518 Size: 648 1919 19 789 359 159 2042 Fragments arranged by size: 2042 1919 789 648 359 159 19 MspA1I CmG'CkG Cuts at: 1606 2188 2710 2791 2954 3351 4028 5176 5421 Size: 582 522 81 163 397 677 1148 245 Cuts at: 5421 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 582 522 397 245 163 81 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4444 Fragments arranged by size: 4444 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5935 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5935 NotI GC'GGCC_GC Cuts at: 3382 3382 Size: 5935 NspI r_CATG'y Cuts at: 1132 1229 3293 4838 1132 Size: 97 2064 1545 2229 Fragments arranged by size: 2229 2064 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5935 PciI A'CATG_T Cuts at: 4834 4834 Size: 5935 PflMI CCAn_nnn'nTGG Cuts at: 1108 2371 3165 3227 1108 Size: 1263 794 62 3816 Fragments arranged by size: 3816 1263 794 62 PfoI T'CCnGG_A Cuts at: 2364 2661 2364 Size: 297 5638 PmeI GTTT'AAAC Cuts at: 3239 3239 Size: 5935 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4004 4036 5517 5549 4004 Size: 32 1481 32 4390 Fragments arranged by size: 4390 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3124 3178 3124 Size: 54 5881 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5935 PsiI TTA'TAA Cuts at: 3505 3505 Size: 5935 PstI C_TGCA'G Cuts at: 830 2387 2709 2784 2831 3287 830 Size: 1557 322 75 47 456 3478 Fragments arranged by size: 3478 1557 456 322 75 47 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5935 PvuII CAG'CTG Cuts at: 2188 2710 2791 2188 Size: 522 81 5332 Fragments arranged by size: 5332 522 81 SalI G'TCGA_C Cuts at: 1936 3277 1936 Size: 1341 4594 SanDI GG'GwC_CC Cuts at: 3178 3178 Size: 5935 SbfI CC_TGCA'GG Cuts at: 2784 3287 2784 Size: 503 5432 ScaI AGT'ACT Cuts at: 4183 5895 4183 Size: 1712 4223 SexAI A'CCwGG_T Cuts at: 2262 2262 Size: 5935 SfcI C'TryA_G Cuts at: 826 1045 2383 2705 2780 2827 2927 3283 4418 Size: 219 1338 322 75 47 100 356 1135 Cuts at: 4418 5099 5290 826 Size: 681 191 1471 Fragments arranged by size: 1471 1338 1135 681 356 322 219 191 100 75 47 SfiI GGCCn_nnn'nGGCC Cuts at: 2103 2103 Size: 5935 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5935 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5935 SmaI CCC'GGG Cuts at: 1872 3262 1872 Size: 1390 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2234 4039 4940 5202 820 Size: 197 926 291 1805 901 262 1553 Fragments arranged by size: 1805 1553 926 901 291 262 197 SnaBI TAC'GTA Cuts at: 493 3726 493 Size: 3233 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5935 SphI G_CATG'C Cuts at: 1132 3293 1132 Size: 2161 3774 SspI AAT'ATT Cuts at: 5 52 3859 5 Size: 47 3807 2081 Fragments arranged by size: 3807 2081 47 StuI AGG'CCT Cuts at: 1517 2130 2285 1517 Size: 613 155 5167 Fragments arranged by size: 5167 613 155 StyI C'CwwG_G Cuts at: 513 1065 2004 2868 3204 3374 513 Size: 552 939 864 336 170 3074 Fragments arranged by size: 3074 939 864 552 336 170 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2455 2954 3962 3979 4132 4317 4762 Size: 1255 875 499 1008 17 153 185 445 Cuts at: 4762 325 Size: 1498 Fragments arranged by size: 1498 1255 1008 875 499 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3198 4181 5893 Size: 274 80 33 51 1443 1221 983 1712 Cuts at: 5893 96 Size: 138 Fragments arranged by size: 1712 1443 1221 983 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2723 2755 5895 5927 2723 Size: 32 3140 32 2731 Fragments arranged by size: 3140 2731 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5935 XbaI T'CTAG_A Cuts at: 3271 3271 Size: 5935 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5935 XmaI C'CCGG_G Cuts at: 1870 3260 1870 Size: 1390 4545 XmnI GAAnn'nnTTC Cuts at: 4064 4064 Size: 5935 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3743 276 Size: 53 83 186 936 2209 2468 Fragments arranged by size: 2468 2209 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SapI SfoI SrfI SwaI XcmI