Restriction map for
pFN21AE0195
(Linear) (Six-base) MAPSORT of: KIEE0195.seq Check: 4562 from: 1 to: 1230
KIEE0195.orf
With 182 enzymes: SgfI *
April 24, 2009 13:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1096 1230
Size: 1096 134
AccI GT'mk_AC
Cuts at: 0 1188 1230
Size: 1188 42
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 706 1230
Size: 706 524
AleI CACnn'nnGTG
Cuts at: 0 1080 1230
Size: 1080 150
AlwNI CAG_nnn'CTG
Cuts at: 0 366 1230
Size: 366 864
ApaLI G'TGCA_C
Cuts at: 0 1083 1230
Size: 1083 147
AscI GG'CGCG_CC
Cuts at: 0 1140 1230
Size: 1140 90
AvaI C'yCGr_G
Cuts at: 0 385 466 1230
Size: 385 81 764
Fragments arranged by size:
764 385 81
BanI G'GyrC_C
Cuts at: 0 155 254 261 1230
Size: 155 99 7 969
Fragments arranged by size:
969 155 99 7
BanII G_rGCy'C
Cuts at: 0 31 464 1230
Size: 31 433 766
Fragments arranged by size:
766 433 31
BbsI GAAGACnn'nnnn_
Cuts at: 0 92 488 1230
Size: 92 396 742
Fragments arranged by size:
742 396 92
BciVI GTATCCnnnnn_n'
Cuts at: 0 249 1230
Size: 249 981
BclI T'GATC_A
Cuts at: 0 1067 1230
Size: 1067 163
BglI GCCn_nnn'nGGC
Cuts at: 0 98 1230
Size: 98 1132
Bme1580I G_kGCm'C
Cuts at: 0 1087 1230
Size: 1087 143
BmgBI CAC'GTC
Cuts at: 0 560 1230
Size: 560 670
BmrI ACTGGGnnnn_n'
Cuts at: 0 89 431 1230
Size: 89 342 799
Fragments arranged by size:
799 342 89
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 343 388 640 733 934 961 1164 1199
Size: 343 45 252 93 201 27 203 35
Cuts at: 1199 1230
Size: 31
Fragments arranged by size:
343 252 203 201 93 45 35 31
27
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 214 1230
Size: 214 1016
BsaI GGTCTCn'nnnn_
Cuts at: 0 692 880 1230
Size: 692 188 350
Fragments arranged by size:
692 350 188
BsaBI GATnn'nnATC
Cuts at: 0 1105 1230
Size: 1105 125
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 718 748 1230
Size: 718 30 482
Fragments arranged by size:
718 482 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 742 1230
Size: 742 488
BseYI C'CCAG_C
Cuts at: 0 31 417 584 1230
Size: 31 386 167 646
Fragments arranged by size:
646 386 167 31
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1074 1230
Size: 1074 156
BsiHKAI G_wGCw'C
Cuts at: 0 332 956 1087 1230
Size: 332 624 131 143
Fragments arranged by size:
624 332 143 131
Bsp1286I G_dGCh'C
Cuts at: 0 31 332 464 956 1087 1230
Size: 31 301 132 492 131 143
Fragments arranged by size:
492 301 143 132 131 31
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 506 605 707 1096 1230
Size: 506 99 102 389 134
Fragments arranged by size:
506 389 134 102 99
BsrFI r'CCGG_y
Cuts at: 0 65 251 489 1230
Size: 65 186 238 741
Fragments arranged by size:
741 238 186 65
BssHII G'CGCG_C
Cuts at: 0 300 1140 1230
Size: 300 840 90
Fragments arranged by size:
840 300 90
BstAPI GCAn_nnn'nTGC
Cuts at: 0 261 708 1230
Size: 261 447 522
Fragments arranged by size:
522 447 261
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 300 1230
Size: 300 930
DraI TTT'AAA
Cuts at: 0 8 1230
Size: 8 1222
DraIII CAC_nnn'GTG
Cuts at: 0 261 439 1046 1230
Size: 261 178 607 184
Fragments arranged by size:
607 261 184 178
DrdI GACnn_nn'nnGTC
Cuts at: 0 490 1230
Size: 490 740
EarI CTCTTCn'nnn_
Cuts at: 0 824 1230
Size: 824 406
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 28 1230
Size: 28 1202
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 343 388 640 706 733 934 961 1164
Size: 343 45 252 66 27 201 27 203
Cuts at: 1164 1199 1230
Size: 35 31
Fragments arranged by size:
343 252 203 201 66 45 35 31
27 27
EcoNI CCTnn'n_nnAGG
Cuts at: 0 724 1131 1230
Size: 724 407 99
Fragments arranged by size:
724 407 99
EcoO109I rG'GnC_Cy
Cuts at: 0 447 515 1119 1173 1230
Size: 447 68 604 54 57
Fragments arranged by size:
604 447 68 57 54
HincII GTy'rAC
Cuts at: 0 495 1230
Size: 495 735
MslI CAynn'nnrTG
Cuts at: 0 1080 1099 1230
Size: 1080 19 131
Fragments arranged by size:
1080 131 19
MspA1I CmG'CkG
Cuts at: 0 183 705 786 949 1230
Size: 183 522 81 163 281
Fragments arranged by size:
522 281 183 163 81
PflMI CCAn_nnn'nTGG
Cuts at: 0 366 1160 1222 1230
Size: 366 794 62 8
Fragments arranged by size:
794 366 62 8
PfoI T'CCnGG_A
Cuts at: 0 359 656 1230
Size: 359 297 574
Fragments arranged by size:
574 359 297
PpuMI rG'GwC_Cy
Cuts at: 0 1119 1173 1230
Size: 1119 54 57
Fragments arranged by size:
1119 57 54
PstI C_TGCA'G
Cuts at: 0 382 704 779 826 1230
Size: 382 322 75 47 404
Fragments arranged by size:
404 382 322 75 47
PvuII CAG'CTG
Cuts at: 0 183 705 786 1230
Size: 183 522 81 444
Fragments arranged by size:
522 444 183 81
SanDI GG'GwC_CC
Cuts at: 0 1173 1230
Size: 1173 57
SbfI CC_TGCA'GG
Cuts at: 0 779 1230
Size: 779 451
SexAI A'CCwGG_T
Cuts at: 0 257 1230
Size: 257 973
SfcI C'TryA_G
Cuts at: 0 378 700 775 822 922 1230
Size: 378 322 75 47 100 308
Fragments arranged by size:
378 322 308 100 75 47
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 98 1230
Size: 98 1132
SmlI C'TyrA_G
Cuts at: 0 229 1230
Size: 229 1001
StuI AGG'CCT
Cuts at: 0 125 280 1230
Size: 125 155 950
Fragments arranged by size:
950 155 125
StyI C'CwwG_G
Cuts at: 0 863 1199 1230
Size: 863 336 31
Fragments arranged by size:
863 336 31
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 450 949 1230
Size: 450 499 281
Fragments arranged by size:
499 450 281
TatI w'GTAC_w
Cuts at: 0 1193 1230
Size: 1193 37
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 718 750 1230
Size: 718 32 480
Fragments arranged by size:
718 480 32
Enzymes that do cut:
AarI AccI AcuI AleI AlwNI ApaLI AscI AvaI
BanI BanII BbsI BciVI BclI BglI Bme1580I BmgBI
BmrI BpmI BpuEI BsaI BsaBI BsaXI BseRI BseYI
BsgI BsiHKAI Bsp1286I BspMI BsrFI BssHII BstAPI BtgZI
DraI DraIII DrdI EarI EciI Eco57MI EcoNI EcoO109I
HincII MslI MspA1I PflMI PfoI PpuMI PstI PvuII
SanDI SbfI SexAI SfcI SfiI SmlI StuI StyI
TaqII TatI TstI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AlfI AloI ApaI ApoI AseI AsiSI AvrII BaeI
BamHI BbeI BbvCI BcgI BfrBI BglII BlpI BmtI
BplI Bpu10I BsaAI BsaHI BsaWI BsiEI BsiWI BsmI
BsmBI BspEI BspHI BsrBI BsrDI BsrGI BssSI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtsI ClaI
CspCI EaeI EagI EcoICRI EcoRI EcoRV FalI FseI
FspI FspAI HaeII Hin4I HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PasI
PciI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacI SacII SalI SapI ScaI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI SwaI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI