(Circular) (Six-base) MAPSORT of: pFN21AB7214.seq Check: 2138 from: 1 to: 5584 pFN21AB7214 5584 bp With 182 enzymes: SgfI * April 15, 2010 09:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2275 2275 Size: 5584 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3394 278 Size: 53 83 186 936 1858 2468 Fragments arranged by size: 2468 1858 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2927 1937 Size: 990 4594 Acc65I G'GTAC_C Cuts at: 2905 2905 Size: 5584 AclI AA'CG_TT Cuts at: 1563 3711 4084 1563 Size: 2148 373 3063 Fragments arranged by size: 3063 2148 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2039 2187 2645 3647 5031 5299 1632 Size: 407 148 458 1002 1384 268 1917 Fragments arranged by size: 1917 1384 1002 458 407 268 148 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5387 AflIII A'CryG_T Cuts at: 1203 4483 5460 1203 Size: 3280 977 1327 Fragments arranged by size: 3280 1327 977 AgeI A'CCGG_T Cuts at: 4415 4415 Size: 5584 AhdI GACnn_n'nnGTC Cuts at: 4313 4313 Size: 5584 AleI CACnn'nnGTG Cuts at: 1166 2218 2345 2432 1166 Size: 1052 127 87 4318 Fragments arranged by size: 4318 1052 127 87 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2380 1184 Size: 1196 4388 AlwNI CAG_nnn'CTG Cuts at: 2165 2604 3327 4899 2165 Size: 439 723 1572 2850 Fragments arranged by size: 2850 1572 723 439 ApaI G_GGCC'C Cuts at: 2177 2177 Size: 5584 ApaLI G'TGCA_C Cuts at: 3641 4797 3641 Size: 1156 4428 ApoI r'AATT_y Cuts at: 1493 2533 2575 2655 2893 3119 3271 5380 1493 Size: 1040 42 80 238 226 152 2109 1697 Fragments arranged by size: 2109 1697 1040 238 226 152 80 42 AseI AT'TA_AT Cuts at: 160 1032 4138 160 Size: 872 3106 1606 Fragments arranged by size: 3106 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5584 AvaI C'yCGr_G Cuts at: 1870 1943 2025 2066 2909 1870 Size: 73 82 41 843 4545 Fragments arranged by size: 4545 843 82 73 41 BamHI G'GATC_C Cuts at: 2914 3281 2914 Size: 367 5217 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2092 2324 2905 4360 618 Size: 325 217 627 305 232 581 1455 1842 Fragments arranged by size: 1842 1455 627 581 325 305 232 217 BanII G_rGCy'C Cuts at: 1391 2153 2177 2903 1391 Size: 762 24 726 4072 Fragments arranged by size: 4072 762 726 24 BbeI G_GCGC'C Cuts at: 2096 2096 Size: 5584 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4613 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3775 3809 1903 Size: 34 1838 34 3678 Fragments arranged by size: 3678 1838 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3476 4686 3476 Size: 1210 4374 BclI T'GATC_A Cuts at: 1548 1548 Size: 5584 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4195 136 Size: 107 122 71 631 605 2523 1525 Fragments arranged by size: 2523 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5579 5579 Size: 5584 BlpI GC'TnA_GC Cuts at: 3001 3001 Size: 5584 Bme1580I G_kGCm'C Cuts at: 1790 2177 3645 4801 1790 Size: 387 1468 1156 2573 Fragments arranged by size: 2573 1468 1156 387 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5584 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2086 3315 4273 Size: 416 53 83 399 24 669 1229 958 Cuts at: 4273 442 Size: 1753 Fragments arranged by size: 1753 1229 958 669 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5584 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2172 2172 Size: 5584 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2142 2498 4244 1239 Size: 903 356 1746 2579 Fragments arranged by size: 2579 1746 903 356 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3709 4574 4872 3709 Size: 865 298 4421 Fragments arranged by size: 4421 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2446 4247 882 Size: 617 947 1801 2219 Fragments arranged by size: 2219 1801 947 617 BsaAI yAC'GTr Cuts at: 493 1204 2343 3375 493 Size: 711 1139 1032 2702 Fragments arranged by size: 2702 1139 1032 711 BsaBI GATnn'nnATC Cuts at: 5578 5578 Size: 5584 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2093 3391 3773 4408 Size: 53 83 186 936 560 1298 382 635 Cuts at: 4408 275 Size: 1451 Fragments arranged by size: 1451 1298 936 635 560 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2612 4017 4415 4689 4836 5247 2612 Size: 1405 398 274 147 411 2949 Fragments arranged by size: 2949 1405 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5359 Fragments arranged by size: 5359 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2105 2138 2265 1186 Size: 919 33 127 4505 Fragments arranged by size: 4505 919 127 33 BseYI C'CCAG_C Cuts at: 2604 2862 4787 5283 2604 Size: 258 1925 496 2905 Fragments arranged by size: 2905 1925 496 258 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5011 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3034 3795 4823 1540 Size: 275 186 1033 761 1028 2301 Fragments arranged by size: 2301 1033 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3645 3730 4801 3645 Size: 85 1071 4428 Fragments arranged by size: 4428 1071 85 BsmI GAATG_Cn' Cuts at: 3095 3188 3095 Size: 93 5491 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2153 2177 2903 3645 3730 4801 1391 Size: 399 363 24 726 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 726 399 363 85 24 BspEI T'CCGG_A Cuts at: 5247 5247 Size: 5584 BspHI T'CATG_A Cuts at: 3473 5203 3473 Size: 1730 3854 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2275 2939 844 Size: 1431 664 3489 Fragments arranged by size: 3489 1431 664 BsrBI CCG'CTC Cuts at: 1394 1823 3471 1394 Size: 429 1648 3507 Fragments arranged by size: 3507 1648 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4079 4253 65 Size: 1183 2831 174 1396 Fragments arranged by size: 2831 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4228 4415 1700 Size: 110 2418 187 2869 Fragments arranged by size: 2869 2418 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5584 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5584 BssSI C'ACGA_G Cuts at: 3644 4656 3644 Size: 1012 4572 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5584 BstBI TT'CG_AA Cuts at: 2494 4388 2494 Size: 1894 3690 BstEII G'GTnAC_C Cuts at: 1184 2332 2608 1184 Size: 1148 276 4160 Fragments arranged by size: 4160 1148 276 BstYI r'GATC_y Cuts at: 1972 2914 3281 3666 3683 5135 5221 5233 5579 Size: 942 367 385 17 1452 86 12 346 Cuts at: 5579 1972 Size: 1977 Fragments arranged by size: 1977 1452 942 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2568 2568 Size: 5584 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5032 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4845 Fragments arranged by size: 4845 683 56 BtsI GCAGTG_nn' Cuts at: 2213 2362 3100 3894 3914 2213 Size: 149 738 794 20 3883 Fragments arranged by size: 3883 794 738 149 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5584 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2418 2453 529 Size: 35 1854 35 3660 Fragments arranged by size: 3660 1854 35 35 DraI TTT'AAA Cuts at: 2888 2888 Size: 5584 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2840 4591 809 Size: 507 1524 1751 1802 Fragments arranged by size: 1802 1751 1524 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2224 3031 3920 Size: 54 1425 50 275 18 394 807 889 Cuts at: 3920 5549 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 807 394 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3031 1537 Size: 275 1219 4090 Fragments arranged by size: 4090 1219 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3514 1353 Size: 2161 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4171 4545 4691 387 Size: 1064 138 2582 374 146 1280 Fragments arranged by size: 2582 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2039 2142 2187 2498 2645 3647 4244 Size: 393 407 103 45 311 147 1002 597 Cuts at: 4244 5031 5299 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1002 787 597 407 393 311 268 147 103 45 EcoNI CCTnn'n_nnAGG Cuts at: 2120 2120 Size: 5584 EcoO109I rG'GnC_Cy Cuts at: 2029 2162 2174 2029 Size: 133 12 5439 Fragments arranged by size: 5439 133 12 EcoRI G'AATT_C Cuts at: 2893 3271 2893 Size: 378 5206 FspI TGC'GCA Cuts at: 4090 4090 Size: 5584 HaeII r_GCGC'y Cuts at: 1678 2096 4731 1678 Size: 418 2635 2531 Fragments arranged by size: 2635 2531 418 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2389 2421 2445 2477 4225 Size: 32 135 32 817 32 24 32 1748 Cuts at: 4225 4257 4299 4331 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1748 817 135 42 32 32 32 32 32 32 24 HincII GTy'rAC Cuts at: 669 1657 1938 2928 3174 669 Size: 988 281 990 246 3079 Fragments arranged by size: 3079 990 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5584 HpaI GTT'AAC Cuts at: 3174 3174 Size: 5584 KasI G'GCGC_C Cuts at: 2092 2092 Size: 5584 KpnI G_GTAC'C Cuts at: 2909 2909 Size: 5584 MfeI C'AATT_G Cuts at: 3183 3183 Size: 5584 MluI A'CGCG_T Cuts at: 5460 5460 Size: 5584 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2545 4698 4882 1305 Size: 1240 2153 184 2007 Fragments arranged by size: 2153 2007 1240 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3762 Fragments arranged by size: 3762 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2218 2345 2388 2432 3542 3901 4060 Size: 648 1052 127 43 44 1110 359 159 Cuts at: 4060 518 Size: 2042 Fragments arranged by size: 2042 1110 1052 648 359 159 127 44 43 MspA1I CmG'CkG Cuts at: 1606 3000 3677 4825 5070 1606 Size: 1394 677 1148 245 2120 Fragments arranged by size: 2120 1394 1148 677 245 NarI GG'CG_CC Cuts at: 2093 2093 Size: 5584 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5032 NdeI CA'TA_TG Cuts at: 387 387 Size: 5584 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5584 NotI GC'GGCC_GC Cuts at: 3031 3031 Size: 5584 NspI r_CATG'y Cuts at: 1132 1229 2942 4487 1132 Size: 97 1713 1545 2229 Fragments arranged by size: 2229 1713 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5584 PciI A'CATG_T Cuts at: 4483 4483 Size: 5584 PflMI CCAn_nnn'nTGG Cuts at: 1108 2604 1108 Size: 1496 4088 PfoI T'CCnGG_A Cuts at: 2158 2158 Size: 5584 PmeI GTTT'AAAC Cuts at: 2888 2888 Size: 5584 PmlI CAC'GTG Cuts at: 2343 2343 Size: 5584 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3653 3685 5166 5198 3653 Size: 32 1481 32 4039 Fragments arranged by size: 4039 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2162 2162 Size: 5584 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5584 PsiI TTA'TAA Cuts at: 3154 3154 Size: 5584 PspOMI G'GGCC_C Cuts at: 2173 2173 Size: 5584 PstI C_TGCA'G Cuts at: 830 2936 830 Size: 2106 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5584 SalI G'TCGA_C Cuts at: 1936 2926 1936 Size: 990 4594 SbfI CC_TGCA'GG Cuts at: 2936 2936 Size: 5584 ScaI AGT'ACT Cuts at: 3832 5544 3832 Size: 1712 3872 SexAI A'CCwGG_T Cuts at: 2597 2597 Size: 5584 SfcI C'TryA_G Cuts at: 826 1045 2932 4067 4748 4939 826 Size: 219 1887 1135 681 191 1471 Fragments arranged by size: 1887 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2094 2094 Size: 5584 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5584 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5584 SmaI CCC'GGG Cuts at: 1872 2027 2068 2911 1872 Size: 155 41 843 4545 Fragments arranged by size: 4545 843 155 41 SmlI C'TyrA_G Cuts at: 820 1017 1943 3688 4589 4851 820 Size: 197 926 1745 901 262 1553 Fragments arranged by size: 1745 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3375 493 Size: 2882 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5584 SphI G_CATG'C Cuts at: 1132 2942 1132 Size: 1810 3774 SrfI GCCC'GGGC Cuts at: 2068 2068 Size: 5584 SspI AAT'ATT Cuts at: 5 52 3508 5 Size: 47 3456 2081 Fragments arranged by size: 3456 2081 47 StuI AGG'CCT Cuts at: 1517 2720 1517 Size: 1203 4381 StyI C'CwwG_G Cuts at: 513 1065 3023 513 Size: 552 1958 3074 Fragments arranged by size: 3074 1958 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2335 2596 3611 3628 3781 3966 4411 Size: 1255 755 261 1015 17 153 185 445 Cuts at: 4411 325 Size: 1498 Fragments arranged by size: 1498 1255 1015 755 445 261 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3830 5542 96 Size: 274 80 33 51 1443 1853 1712 138 Fragments arranged by size: 1853 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2258 2290 2354 2386 2746 2778 5544 5576 2258 Size: 32 64 32 360 32 2766 32 2266 Fragments arranged by size: 2766 2266 360 64 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5584 XbaI T'CTAG_A Cuts at: 2920 2920 Size: 5584 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5584 XmaI C'CCGG_G Cuts at: 1870 2025 2066 2909 1870 Size: 155 41 843 4545 Fragments arranged by size: 4545 843 155 41 XmnI GAAnn'nnTTC Cuts at: 2658 2785 3713 2658 Size: 127 928 4529 Fragments arranged by size: 4529 928 127 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3392 276 Size: 53 83 186 936 1858 2468 Fragments arranged by size: 2468 1858 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SfiI SwaI XcmI