(Circular) (Six-base) MAPSORT of: pFN21AB7767.seq Check: 2254 from: 1 to: 6046 pFN21AB7767 6046 bp With 182 enzymes: SgfI * October 20, 2009 10:55 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3052 3052 Size: 6046 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2817 3856 278 Size: 53 83 186 936 1281 1039 2468 Fragments arranged by size: 2468 1281 1039 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3389 1937 Size: 1452 4594 Acc65I G'GTAC_C Cuts at: 2081 3367 2081 Size: 1286 4760 AclI AA'CG_TT Cuts at: 1563 4173 4546 1563 Size: 2610 373 3063 Fragments arranged by size: 3063 2610 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4109 5493 5761 1632 Size: 2477 1384 268 1917 Fragments arranged by size: 2477 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5849 AflIII A'CryG_T Cuts at: 1203 4945 5922 1203 Size: 3742 977 1327 Fragments arranged by size: 3742 1327 977 AgeI A'CCGG_T Cuts at: 4877 4877 Size: 6046 AhdI GACnn_n'nnGTC Cuts at: 4775 4775 Size: 6046 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6046 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6046 AlwNI CAG_nnn'CTG Cuts at: 2049 3262 3789 5361 2049 Size: 1213 527 1572 2734 Fragments arranged by size: 2734 1572 1213 527 ApaI G_GGCC'C Cuts at: 2120 2514 2933 3094 3316 2120 Size: 394 419 161 222 4850 Fragments arranged by size: 4850 419 394 222 161 ApaLI G'TGCA_C Cuts at: 2759 4103 5259 2759 Size: 1344 1156 3546 Fragments arranged by size: 3546 1344 1156 ApoI r'AATT_y Cuts at: 1493 2834 3157 3355 3581 3733 5842 1493 Size: 1341 323 198 226 152 2109 1697 Fragments arranged by size: 2109 1697 1341 323 226 198 152 AseI AT'TA_AT Cuts at: 160 1032 4600 160 Size: 872 3568 1606 Fragments arranged by size: 3568 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6046 AvaI C'yCGr_G Cuts at: 1870 1943 2016 2664 2801 3371 1870 Size: 73 73 648 137 570 4545 Fragments arranged by size: 4545 648 570 137 73 73 BamHI G'GATC_C Cuts at: 3376 3743 3376 Size: 367 5679 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2081 2675 2748 3367 4822 Size: 325 217 627 294 594 73 619 1455 Cuts at: 4822 618 Size: 1842 Fragments arranged by size: 1842 1455 627 619 594 325 294 217 73 BanII G_rGCy'C Cuts at: 1391 2120 2144 2514 2607 2933 3094 3316 3365 Size: 729 24 370 93 326 161 222 49 Cuts at: 3365 1391 Size: 4072 Fragments arranged by size: 4072 729 370 326 222 161 93 49 24 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2288 3277 928 Size: 971 389 989 3697 Fragments arranged by size: 3697 989 971 389 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4237 4271 1903 Size: 34 2300 34 3678 Fragments arranged by size: 3678 2300 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3938 5148 3938 Size: 1210 4836 BclI T'GATC_A Cuts at: 1548 1548 Size: 6046 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2660 4657 136 Size: 107 122 71 631 605 988 1997 1525 Fragments arranged by size: 1997 1525 988 631 605 122 107 71 BglII A'GATC_T Cuts at: 2823 6041 2823 Size: 3218 2828 BlpI GC'TnA_GC Cuts at: 3463 3463 Size: 6046 Bme1580I G_kGCm'C Cuts at: 1790 2120 2514 2678 2763 2933 3094 3316 4107 Size: 330 394 164 85 170 161 222 791 Cuts at: 4107 5263 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 791 394 330 222 170 164 161 85 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6046 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2462 2935 3777 Size: 416 53 83 399 24 1045 473 842 Cuts at: 3777 4735 442 Size: 958 1753 Fragments arranged by size: 1753 1045 958 842 473 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6046 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2520 3119 3215 4706 1239 Size: 1281 599 96 1491 2579 Fragments arranged by size: 2579 1491 1281 599 96 Bpu10I CC'TnA_GC Cuts at: 3186 3186 Size: 6046 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4171 5036 5334 4171 Size: 865 298 4883 Fragments arranged by size: 4883 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3298 4709 882 Size: 617 1799 1411 2219 Fragments arranged by size: 2219 1799 1411 617 BsaAI yAC'GTr Cuts at: 493 1204 3837 493 Size: 711 2633 2702 Fragments arranged by size: 2702 2633 711 BsaBI GATnn'nnATC Cuts at: 6040 6040 Size: 6046 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2814 3853 4235 4870 Size: 53 83 186 936 1281 1039 382 635 Cuts at: 4870 275 Size: 1451 Fragments arranged by size: 1451 1281 1039 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4479 4877 5151 5298 5709 4479 Size: 398 274 147 411 4816 Fragments arranged by size: 4816 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5821 Fragments arranged by size: 5821 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2090 2957 1186 Size: 904 867 4275 Fragments arranged by size: 4275 904 867 BseYI C'CCAG_C Cuts at: 2021 2102 2418 2650 2780 3093 3315 5249 5745 Size: 81 316 232 130 313 222 1934 496 Cuts at: 5745 2021 Size: 2322 Fragments arranged by size: 2322 1934 496 316 313 232 222 130 81 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3256 1163 Size: 573 1520 3953 Fragments arranged by size: 3953 1520 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2197 3496 4257 5285 1540 Size: 275 186 196 1299 761 1028 2301 Fragments arranged by size: 2301 1299 1028 761 275 196 186 BsiHKAI G_wGCw'C Cuts at: 2763 4107 4192 5263 2763 Size: 1344 85 1071 3546 Fragments arranged by size: 3546 1344 1071 85 BsmI GAATG_Cn' Cuts at: 3557 3650 3557 Size: 93 5953 BsmBI CGTCTCn'nnnn_ Cuts at: 3074 3074 Size: 6046 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2120 2144 2514 2607 2678 2763 2933 Size: 399 330 24 370 93 71 85 170 Cuts at: 2933 3094 3316 3365 4107 4192 5263 1391 Size: 161 222 49 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 370 330 222 170 161 93 85 85 71 49 24 BspEI T'CCGG_A Cuts at: 5709 5709 Size: 6046 BspHI T'CATG_A Cuts at: 3935 5665 3935 Size: 1730 4316 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3052 3401 844 Size: 2208 349 3489 Fragments arranged by size: 3489 2208 349 BsrBI CCG'CTC Cuts at: 1394 1823 2800 3933 1394 Size: 429 977 1133 3507 Fragments arranged by size: 3507 1133 977 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4541 4715 65 Size: 1183 3293 174 1396 Fragments arranged by size: 3293 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2084 2954 4690 4877 1700 Size: 110 274 870 1736 187 2869 Fragments arranged by size: 2869 1736 870 274 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6046 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6046 BssSI C'ACGA_G Cuts at: 4106 5118 4106 Size: 1012 5034 BstAPI GCAn_nnn'nTGC Cuts at: 794 3172 794 Size: 2378 3668 BstBI TT'CG_AA Cuts at: 4850 4850 Size: 6046 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6046 BstYI r'GATC_y Cuts at: 1972 2504 2823 3376 3743 4128 4145 5597 5683 Size: 532 319 553 367 385 17 1452 86 Cuts at: 5683 5695 6041 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 553 532 385 367 346 319 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2469 2469 Size: 6046 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4555 Fragments arranged by size: 4555 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2628 487 Size: 683 56 1402 3905 Fragments arranged by size: 3905 1402 683 56 BtsI GCAGTG_nn' Cuts at: 2065 2077 2098 2773 2977 3562 4356 4376 2065 Size: 12 21 675 204 585 794 20 3735 Fragments arranged by size: 3735 794 675 585 204 21 20 12 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6046 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6011 DraI TTT'AAA Cuts at: 3350 3350 Size: 6046 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5053 809 Size: 507 3737 1802 Fragments arranged by size: 3737 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2175 2194 3493 Size: 54 1425 50 275 18 345 19 1299 Cuts at: 3493 4382 6011 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1299 889 345 275 54 50 43 19 18 EagI C'GGCC_G Cuts at: 1537 1812 2194 3493 1537 Size: 275 382 1299 4090 Fragments arranged by size: 4090 1299 382 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3976 1353 Size: 2623 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2930 4633 5007 5153 387 Size: 1064 138 1341 1703 374 146 1280 Fragments arranged by size: 1703 1341 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2520 3119 3215 4109 4706 5493 5761 Size: 393 888 599 96 894 597 787 268 Cuts at: 5761 1239 Size: 1524 Fragments arranged by size: 1524 894 888 787 599 597 393 268 96 EcoO109I rG'GnC_Cy Cuts at: 2116 2170 2456 2511 2668 2929 3312 2116 Size: 54 286 55 157 261 383 4850 Fragments arranged by size: 4850 383 286 261 157 55 54 EcoRI G'AATT_C Cuts at: 3355 3733 3355 Size: 378 5668 FspI TGC'GCA Cuts at: 4552 4552 Size: 6046 HaeII r_GCGC'y Cuts at: 1678 2795 5193 1678 Size: 1117 2398 2531 Fragments arranged by size: 2531 2398 1117 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2221 2253 2275 2307 4687 Size: 32 135 32 649 32 22 32 2380 Cuts at: 4687 4719 4761 4793 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2380 649 135 42 32 32 32 32 32 32 22 HincII GTy'rAC Cuts at: 669 1657 1938 3390 3636 669 Size: 988 281 1452 246 3079 Fragments arranged by size: 3079 1452 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6046 HpaI GTT'AAC Cuts at: 3636 3636 Size: 6046 KpnI G_GTAC'C Cuts at: 2085 3371 2085 Size: 1286 4760 MfeI C'AATT_G Cuts at: 3645 3645 Size: 6046 MluI A'CGCG_T Cuts at: 5922 5922 Size: 6046 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2219 5160 5344 1305 Size: 914 2941 184 2007 Fragments arranged by size: 2941 2007 914 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2177 10 Size: 54 1425 343 345 3879 Fragments arranged by size: 3879 1425 345 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4004 4363 4522 518 Size: 648 2838 359 159 2042 Fragments arranged by size: 2838 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2046 2692 3097 3181 3462 4139 5287 5532 Size: 440 646 405 84 281 677 1148 245 Cuts at: 5532 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 646 440 405 281 245 84 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4555 Fragments arranged by size: 4555 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6046 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6046 NotI GC'GGCC_GC Cuts at: 2194 3493 2194 Size: 1299 4747 NspI r_CATG'y Cuts at: 1132 1229 3404 4949 1132 Size: 97 2175 1545 2229 Fragments arranged by size: 2229 2175 1545 97 PasI CC'CwG_GG Cuts at: 1348 2594 1348 Size: 1246 4800 PciI A'CATG_T Cuts at: 4945 4945 Size: 6046 PflMI CCAn_nnn'nTGG Cuts at: 1108 2323 2398 1108 Size: 1215 75 4756 Fragments arranged by size: 4756 1215 75 PfoI T'CCnGG_A Cuts at: 2163 3210 2163 Size: 1047 4999 PmeI GTTT'AAAC Cuts at: 3350 3350 Size: 6046 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3252 3284 4115 4147 5628 5660 3252 Size: 32 831 32 1481 32 3638 Fragments arranged by size: 3638 1481 831 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2456 2456 Size: 6046 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6046 PsiI TTA'TAA Cuts at: 3616 3616 Size: 6046 PspOMI G'GGCC_C Cuts at: 2116 2510 2929 3090 3312 2116 Size: 394 419 161 222 4850 Fragments arranged by size: 4850 419 394 222 161 PstI C_TGCA'G Cuts at: 830 2445 3398 830 Size: 1615 953 3478 Fragments arranged by size: 3478 1615 953 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6046 PvuII CAG'CTG Cuts at: 2692 3097 3181 2692 Size: 405 84 5557 Fragments arranged by size: 5557 405 84 SalI G'TCGA_C Cuts at: 1936 3388 1936 Size: 1452 4594 SanDI GG'GwC_CC Cuts at: 2456 2456 Size: 6046 SbfI CC_TGCA'GG Cuts at: 3398 3398 Size: 6046 ScaI AGT'ACT Cuts at: 4294 6006 4294 Size: 1712 4334 SexAI A'CCwGG_T Cuts at: 2367 3150 2367 Size: 783 5263 SfcI C'TryA_G Cuts at: 826 1045 2441 3394 4529 5210 5401 826 Size: 219 1396 953 1135 681 191 1471 Fragments arranged by size: 1471 1396 1135 953 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6046 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6046 SmaI CCC'GGG Cuts at: 1872 2018 3373 1872 Size: 146 1355 4545 Fragments arranged by size: 4545 1355 146 SmlI C'TyrA_G Cuts at: 820 1017 1943 2801 4150 5051 5313 820 Size: 197 926 858 1349 901 262 1553 Fragments arranged by size: 1553 1349 926 901 858 262 197 SnaBI TAC'GTA Cuts at: 493 3837 493 Size: 3344 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6046 SphI G_CATG'C Cuts at: 1132 3404 1132 Size: 2272 3774 SspI AAT'ATT Cuts at: 5 52 3970 5 Size: 47 3918 2081 Fragments arranged by size: 3918 2081 47 StuI AGG'CCT Cuts at: 1517 2474 1517 Size: 957 5089 StyI C'CwwG_G Cuts at: 513 1065 2004 2376 3039 3485 513 Size: 552 939 372 663 446 3074 Fragments arranged by size: 3074 939 663 552 446 372 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3269 4073 4090 4243 4428 4873 325 Size: 1255 1689 804 17 153 185 445 1498 Fragments arranged by size: 1689 1498 1255 804 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4292 6004 96 Size: 274 80 33 51 1443 2315 1712 138 Fragments arranged by size: 2315 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2044 2076 6006 6038 2044 Size: 32 3930 32 2052 Fragments arranged by size: 3930 2052 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6046 XbaI T'CTAG_A Cuts at: 3382 3382 Size: 6046 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2495 3232 2495 Size: 737 5309 XhoI C'TCGA_G Cuts at: 1943 2801 1943 Size: 858 5188 XmaI C'CCGG_G Cuts at: 1870 2016 3371 1870 Size: 146 1355 4545 Fragments arranged by size: 4545 1355 146 XmnI GAAnn'nnTTC Cuts at: 2540 4175 2540 Size: 1635 4411 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2815 3854 276 Size: 53 83 186 936 1281 1039 2468 Fragments arranged by size: 2468 1281 1039 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SapI SfiI SfoI SrfI SwaI