Restriction map for
pFN21AE0015
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# NO CUT ENZYMES ( 144 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
Alw44I AocI Aor51HI ApaLI AscI
AseI AsnI Asp700I Asp718I AspHI
AspI AviII AvrII BamHI BbeI
BbrPI BbvCI BcgI BciVI BclI
BfrI BglI BlnI BlpI Bpu1102I
BsaAI BsaHI BsaI BsaWI BseAI
BsgI BsiEI BsiHKAI BsiWI BsmBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstUI BstXI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DraIII DrdI DsaI
EagI EclXI Eco47III Eco52I Eco81I
EcoRI EcoRV EcoT14I EcoT22I FauI
FbaI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HpaI KasI
KpnI KspI MfeI MluI MroI
MunI MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI SexAI SfiI SfoI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI StuI StyI SwaI Tth111I
Van91I XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 34 enzymes) #
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AhdI 18 GACTGATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 1588 GGGCCC GGGCC^C
AspEI 18 GACTGATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 1940 CCCGGG C^[CT]CG[GA]G
BalI 1890 TGGCCA TGG^CCA
BanI 1849 GGCACC G^G[CT][GA]CC
BsaBI 937 GATAATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 489 GAATGC GAATGC(1/-1)
BsoBI 1940 CCCGGG C^[CT]CG[GA]G
BsrDI 341 CATTGC GCAATG(2/0)
BstBI 1454 TTCGAA TT^CGAA
BstEII 499 GGTAACC G^GT[ACGT]ACC
BstF5I 955 CATCC GGATG(2/0)
BstPI 499 GGTAACC G^GT[ACGT]ACC
DraI 1207 TTTAAA TTT^AAA
Eam1105I 18 GACTGATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 727 CCTCTGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 499 GGTAACC G^GT[ACGT]ACC
FokI 955 CATCC GGATG(9/13)
MamI 937 GATAATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1890 TGGCCA TGG^CCA
MscI 1890 TGGCCA TGG^CCA
MspA1I 1550 CAGCTG C[AC]G^C[GT]G
NspV 1454 TTCGAA TT^CGAA
PvuII 1550 CAGCTG CAG^CTG
ScaI 1084 AGTACT AGT^ACT
SfaNI 631 GCATC GCATC(5/9)
SfuI 1454 TTCGAA TT^CGAA
SmaI 1940 CCCGGG CCC^GGG
SmlI 1286 CTCAAG C^T[CT][GA]AG
Tsp45I 1546 GTCAC ^GT[GC]AC
XbaI 1144 TCTAGA T^CTAGA
XmaCI 1940 CCCGGG C^CCGGG
XmaI 1940 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 25 enzymes) #
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AciI 265 CCGC CCGC(-3/-1)
997 CCGC
AlwI 1904 GATCC GGATC(4/5)
1291 GGATC
AlwNI 1094 CAGTATCTG CAG[ACGT][ACGT][ACGT]^CTG
1538 CAGGACCTG
BanII 1588 GGGCCC G[GA]GC[CT]^C
1943 GGGCTC
BglII 763 AGATCT A^GATCT
1600 AGATCT
BmyI 1588 GGGCCC G[AGT]GC[ACT]^C
1943 GGGCTC
BpmI 1673 CTGGAG CTGGAG(16/14)
1867 CTGGAG
BsmAI 951 GAGAC GTCTC(1/5)
1013 GAGAC
BsmFI 894 GGGAC GGGAC(10/14)
1250 GTCCC
Bsp1286I 1588 GGGCCC G[AGT]GC[ACT]^C
1943 GGGCTC
BsrI 576 ACTGG ACTGG(1/-1)
1450 CCAGT
EaeI 1890 TGGCCA [CT]^GGCC[GA]
1912 CGGCCA
EarI 775 GAAGAG CTCTTC(1/4)
1792 GAAGAG
HapII 1719 CCGG C^CGG
1941 CCGG
HincII 118 GTTGAC GT[CT]^[GA]AC
534 GTCAAC
HindII 118 GTTGAC GT[CT]^[GA]AC
534 GTCAAC
HindIII 216 AAGCTT A^AGCTT
1391 AAGCTT
HpaII 1719 CCGG C^CGG
1941 CCGG
HphI 175 GGTGA GGTGA(8/7)
1885 GGTGA
Ksp632I 775 GAAGAG CTCTTC(1/4)
1792 GAAGAG
MaeII 683 ACGT A^CGT
1317 ACGT
MspI 1719 CCGG C^CGG
1941 CCGG
PleI 151 GACTC GAGTC(4/5)
1015 GACTC
SfcI 567 CTATAG C^T[GA][CT]AG
1295 CTGTAG
SspI 241 AATATT AAT^ATT
1572 AATATT
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# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AcsI AfaI AluI ApoI AvaII
BbsI BbvI BcnI BfaI BpuAI
BsaJI BseRI BsiYI BslI BssKI
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraII EcoO109I EcoRII Fnu4HI
HaeIII HinfI ItaI MaeI MaeIII
MboI MboII MflI MnlI MseI
MslI MvaI MwoI NciI NdeII
NlaIII NlaIV PpuMI RsaI Sau3AI
Sau96I ScrFI TaqI TfiI Tru9I
TseI Tsp509I TspRI TthHB8I XhoII