(Circular) (Six-base) MAPSORT of: pFN21AE0015.seq Check: 9730 from: 1 to: 6658 pFN21AE0015 6658 bp With 182 enzymes: SgfI * August 16, 2010 14:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4468 278 Size: 53 83 186 936 2932 2468 Fragments arranged by size: 2932 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4001 1937 Size: 2064 4594 Acc65I G'GTAC_C Cuts at: 3979 3979 Size: 6658 AclI AA'CG_TT Cuts at: 1563 3957 4785 5158 1563 Size: 2394 828 373 3063 Fragments arranged by size: 3063 2394 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2277 2442 2608 2621 2793 3081 3126 3138 Size: 645 165 166 13 172 288 45 12 Cuts at: 3138 4721 6105 6373 1632 Size: 1583 1384 268 1917 Fragments arranged by size: 1917 1583 1384 645 288 268 172 166 165 45 13 12 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6461 AflIII A'CryG_T Cuts at: 1203 5557 6534 1203 Size: 4354 977 1327 Fragments arranged by size: 4354 1327 977 AgeI A'CCGG_T Cuts at: 5489 5489 Size: 6658 AhdI GACnn_n'nnGTC Cuts at: 2028 5387 2028 Size: 3359 3299 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6658 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6658 AlwNI CAG_nnn'CTG Cuts at: 3104 3548 4401 5973 3104 Size: 444 853 1572 3789 Fragments arranged by size: 3789 1572 853 444 ApaI G_GGCC'C Cuts at: 3597 3597 Size: 6658 ApaLI G'TGCA_C Cuts at: 4715 5871 4715 Size: 1156 5502 ApoI r'AATT_y Cuts at: 1493 2054 2390 3028 3773 3967 4193 4345 6454 Size: 561 336 638 745 194 226 152 2109 Cuts at: 6454 1493 Size: 1697 Fragments arranged by size: 2109 1697 745 638 561 336 226 194 152 AseI AT'TA_AT Cuts at: 160 1032 5212 160 Size: 872 4180 1606 Fragments arranged by size: 4180 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6658 AvaI C'yCGr_G Cuts at: 1870 1943 3945 3983 1870 Size: 73 2002 38 4545 Fragments arranged by size: 4545 2002 73 38 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3543 3576 3543 Size: 33 6625 BamHI G'GATC_C Cuts at: 3988 4355 3988 Size: 367 6291 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3854 3979 5434 618 Size: 325 217 627 2067 125 1455 1842 Fragments arranged by size: 2067 1842 1455 627 325 217 125 BanII G_rGCy'C Cuts at: 1391 3597 3952 3977 1391 Size: 2206 355 25 4072 Fragments arranged by size: 4072 2206 355 25 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3126 3523 3714 928 Size: 971 1227 397 191 3872 Fragments arranged by size: 3872 1227 971 397 191 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4849 4883 1903 Size: 34 50 34 2828 34 3678 Fragments arranged by size: 3678 2828 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4550 5760 4550 Size: 1210 5448 BclI T'GATC_A Cuts at: 1548 1548 Size: 6658 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5269 136 Size: 107 122 71 631 605 3597 1525 Fragments arranged by size: 3597 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2768 3605 6653 2768 Size: 837 3048 2773 Fragments arranged by size: 3048 2773 837 BlpI GC'TnA_GC Cuts at: 4075 4075 Size: 6658 Bme1580I G_kGCm'C Cuts at: 1790 3597 4719 5875 1790 Size: 1807 1122 1156 2573 Fragments arranged by size: 2573 1807 1156 1122 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6658 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4389 5347 442 Size: 416 53 83 399 24 2972 958 1753 Fragments arranged by size: 2972 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6658 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2664 2979 2664 Size: 315 6343 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3699 3893 5318 1239 Size: 2460 194 1425 2579 Fragments arranged by size: 2579 2460 1425 194 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3276 4783 5648 5946 3276 Size: 1507 865 298 3988 Fragments arranged by size: 3988 1507 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5321 882 Size: 617 3822 2219 Fragments arranged by size: 3822 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4449 493 Size: 711 3245 2702 Fragments arranged by size: 3245 2702 711 BsaBI GATnn'nnATC Cuts at: 2946 6652 2946 Size: 3706 2952 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4465 4847 5482 275 Size: 53 83 186 936 2932 382 635 1451 Fragments arranged by size: 2932 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5091 5489 5763 5910 6321 5091 Size: 398 274 147 411 5428 Fragments arranged by size: 5428 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6433 Fragments arranged by size: 6433 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2472 2721 2955 1186 Size: 1286 249 234 4889 Fragments arranged by size: 4889 1286 249 234 BseYI C'CCAG_C Cuts at: 5861 6357 5861 Size: 496 6162 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6085 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4108 4869 5897 1540 Size: 275 186 2107 761 1028 2301 Fragments arranged by size: 2301 2107 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4719 4804 5875 4719 Size: 85 1071 5502 Fragments arranged by size: 5502 1071 85 BsmI GAATG_Cn' Cuts at: 2500 4169 4262 2500 Size: 1669 93 4896 Fragments arranged by size: 4896 1669 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3597 3952 3977 4719 4804 5875 1391 Size: 399 1807 355 25 742 85 1071 2174 Fragments arranged by size: 2174 1807 1071 742 399 355 85 25 BspEI T'CCGG_A Cuts at: 6321 6321 Size: 6658 BspHI T'CATG_A Cuts at: 4547 6277 4547 Size: 1730 4928 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4013 844 Size: 3169 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4545 1394 Size: 429 2722 3507 Fragments arranged by size: 3507 2722 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2345 5153 5327 65 Size: 1183 1097 2808 174 1396 Fragments arranged by size: 2808 1396 1183 1097 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5302 5489 1700 Size: 110 3492 187 2869 Fragments arranged by size: 3492 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6658 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6658 BssSI C'ACGA_G Cuts at: 4718 5730 4718 Size: 1012 5646 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6658 BstBI TT'CG_AA Cuts at: 3460 5462 3460 Size: 2002 4656 BstEII G'GTnAC_C Cuts at: 1184 2504 1184 Size: 1320 5338 BstYI r'GATC_y Cuts at: 1972 2768 3296 3605 3988 4355 4740 4757 6209 Size: 796 528 309 383 367 385 17 1452 Cuts at: 6209 6295 6307 6653 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 796 528 385 383 367 346 309 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6106 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5919 Fragments arranged by size: 5919 683 56 BtsI GCAGTG_nn' Cuts at: 3058 4174 4968 4988 3058 Size: 1116 794 20 4728 Fragments arranged by size: 4728 1116 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6658 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2155 2190 529 Size: 35 1591 35 4997 Fragments arranged by size: 4997 1591 35 35 DraI TTT'AAA Cuts at: 3214 3962 3214 Size: 748 5910 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5665 809 Size: 507 4349 1802 Fragments arranged by size: 4349 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3895 3917 4105 Size: 54 1425 50 275 18 2065 22 188 Cuts at: 4105 4994 6623 8 Size: 889 1629 43 Fragments arranged by size: 2065 1629 1425 889 275 188 54 50 43 22 18 EagI C'GGCC_G Cuts at: 1537 1812 4105 1537 Size: 275 2293 4090 Fragments arranged by size: 4090 2293 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2775 3792 4588 1353 Size: 1422 1017 796 3423 Fragments arranged by size: 3423 1422 1017 796 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5245 5619 5765 387 Size: 1064 138 3656 374 146 1280 Fragments arranged by size: 3656 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2277 2442 2608 2621 2793 3081 3126 Size: 393 645 165 166 13 172 288 45 Cuts at: 3126 3138 3699 3893 4721 5318 6105 6373 1239 Size: 12 561 194 828 597 787 268 1524 Fragments arranged by size: 1524 828 787 645 597 561 393 288 268 194 172 166 165 45 13 12 EcoNI CCTnn'n_nnAGG Cuts at: 2736 2736 Size: 6658 EcoO109I rG'GnC_Cy Cuts at: 2726 2785 3254 3527 3545 2726 Size: 59 469 273 18 5839 Fragments arranged by size: 5839 469 273 59 18 EcoRI G'AATT_C Cuts at: 3967 4345 3967 Size: 378 6280 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3111 3111 Size: 6658 FspI TGC'GCA Cuts at: 5164 5164 Size: 6658 HaeII r_GCGC'y Cuts at: 1678 5805 1678 Size: 4127 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2014 2046 2130 2162 3359 Size: 32 135 32 442 32 84 32 1197 Cuts at: 3359 3391 5299 5331 5373 5405 1373 Size: 32 1908 32 42 32 2626 Fragments arranged by size: 2626 1908 1197 442 135 84 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2125 2541 4002 4248 669 Size: 988 281 187 416 1461 246 3079 Fragments arranged by size: 3079 1461 988 416 281 246 187 HindIII A'AGCT_T Cuts at: 748 2221 3396 748 Size: 1473 1175 4010 Fragments arranged by size: 4010 1473 1175 HpaI GTT'AAC Cuts at: 4248 4248 Size: 6658 KpnI G_GTAC'C Cuts at: 3983 3983 Size: 6658 MfeI C'AATT_G Cuts at: 4257 4257 Size: 6658 MluI A'CGCG_T Cuts at: 6534 6534 Size: 6658 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5772 5956 1305 Size: 4467 184 2007 Fragments arranged by size: 4467 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3897 10 Size: 54 1425 343 2065 2771 Fragments arranged by size: 2771 2065 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2565 3201 3429 4616 4975 5134 518 Size: 648 1399 636 228 1187 359 159 2042 Fragments arranged by size: 2042 1399 1187 648 636 359 228 159 MspA1I CmG'CkG Cuts at: 1606 3557 4074 4751 5899 6144 1606 Size: 1951 517 677 1148 245 2120 Fragments arranged by size: 2120 1951 1148 677 517 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6106 NdeI CA'TA_TG Cuts at: 387 387 Size: 6658 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6658 NotI GC'GGCC_GC Cuts at: 4105 4105 Size: 6658 NspI r_CATG'y Cuts at: 1132 1229 4016 5561 1132 Size: 97 2787 1545 2229 Fragments arranged by size: 2787 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6658 PciI A'CATG_T Cuts at: 5557 5557 Size: 6658 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6658 PmeI GTTT'AAAC Cuts at: 3962 3962 Size: 6658 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4727 4759 6240 6272 4727 Size: 32 1481 32 5113 Fragments arranged by size: 5113 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2726 2785 3254 3527 3545 2726 Size: 59 469 273 18 5839 Fragments arranged by size: 5839 469 273 59 18 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6658 PsiI TTA'TAA Cuts at: 3309 4228 3309 Size: 919 5739 PspOMI G'GGCC_C Cuts at: 3593 3593 Size: 6658 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3154 3186 3154 Size: 32 6626 PstI C_TGCA'G Cuts at: 830 4010 830 Size: 3180 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6658 PvuII CAG'CTG Cuts at: 3557 3557 Size: 6658 SalI G'TCGA_C Cuts at: 1936 4000 1936 Size: 2064 4594 SbfI CC_TGCA'GG Cuts at: 4010 4010 Size: 6658 ScaI AGT'ACT Cuts at: 3091 4906 6618 3091 Size: 1815 1712 3131 Fragments arranged by size: 3131 1815 1712 SfcI C'TryA_G Cuts at: 826 1045 2572 3300 4006 5141 5822 6013 826 Size: 219 1527 728 706 1135 681 191 1471 Fragments arranged by size: 1527 1471 1135 728 706 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6658 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6658 SmaI CCC'GGG Cuts at: 1872 3947 3985 1872 Size: 2075 38 4545 Fragments arranged by size: 4545 2075 38 SmlI C'TyrA_G Cuts at: 820 1017 1943 3291 4762 5663 5925 820 Size: 197 926 1348 1471 901 262 1553 Fragments arranged by size: 1553 1471 1348 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4449 493 Size: 3956 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6658 SphI G_CATG'C Cuts at: 1132 4016 1132 Size: 2884 3774 SspI AAT'ATT Cuts at: 5 52 2248 3579 4582 5 Size: 47 2196 1331 1003 2081 Fragments arranged by size: 2196 2081 1331 1003 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6658 StyI C'CwwG_G Cuts at: 513 1065 4097 513 Size: 552 3032 3074 Fragments arranged by size: 3074 3032 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4685 4702 4855 5040 5485 325 Size: 1255 3105 17 153 185 445 1498 Fragments arranged by size: 3105 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3089 4904 6616 Size: 274 80 33 51 1443 1112 1815 1712 Cuts at: 6616 96 Size: 138 Fragments arranged by size: 1815 1712 1443 1112 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6618 6650 6618 Size: 32 6626 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6658 XbaI T'CTAG_A Cuts at: 3149 3994 3149 Size: 845 5813 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6658 XmaI C'CCGG_G Cuts at: 1870 3945 3983 1870 Size: 2075 38 4545 Fragments arranged by size: 4545 2075 38 XmnI GAAnn'nnTTC Cuts at: 4787 4787 Size: 6658 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4466 276 Size: 53 83 186 936 2932 2468 Fragments arranged by size: 2932 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI