Restriction map for
pFN21AE0015
(Linear) (Six-base) MAPSORT of: KIEE0015.seq Check: 3522 from: 1 to: 1953
KIEE0015.orf
With 182 enzymes: SgfI *
March 2, 2010 14:30 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 272 437 603 616 788 1076 1121 1133
Size: 272 165 166 13 172 288 45 12
Cuts at: 1133 1953
Size: 820
Fragments arranged by size:
820 288 272 172 166 165 45 13
12
AhdI GACnn_n'nnGTC
Cuts at: 0 23 1953
Size: 23 1930
AlwNI CAG_nnn'CTG
Cuts at: 0 1099 1543 1953
Size: 1099 444 410
Fragments arranged by size:
1099 444 410
ApaI G_GGCC'C
Cuts at: 0 1592 1953
Size: 1592 361
ApoI r'AATT_y
Cuts at: 0 49 385 1023 1768 1953
Size: 49 336 638 745 185
Fragments arranged by size:
745 638 336 185 49
AvaI C'yCGr_G
Cuts at: 0 1940 1953
Size: 1940 13
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1538 1571 1953
Size: 1538 33 382
Fragments arranged by size:
1538 382 33
BanI G'GyrC_C
Cuts at: 0 1849 1953
Size: 1849 104
BanII G_rGCy'C
Cuts at: 0 1592 1947 1953
Size: 1592 355 6
Fragments arranged by size:
1592 355 6
BbsI GAAGACnn'nnnn_
Cuts at: 0 1121 1518 1709 1953
Size: 1121 397 191 244
Fragments arranged by size:
1121 397 244 191
BglII A'GATC_T
Cuts at: 0 763 1600 1953
Size: 763 837 353
Fragments arranged by size:
837 763 353
Bme1580I G_kGCm'C
Cuts at: 0 1592 1953
Size: 1592 361
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 659 974 1953
Size: 659 315 979
Fragments arranged by size:
979 659 315
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1694 1888 1953
Size: 1694 194 65
Fragments arranged by size:
1694 194 65
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1271 1953
Size: 1271 682
BsaBI GATnn'nnATC
Cuts at: 0 941 1953
Size: 941 1012
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 467 716 950 1953
Size: 467 249 234 1003
Fragments arranged by size:
1003 467 249 234
BsmI GAATG_Cn'
Cuts at: 0 495 1953
Size: 495 1458
Bsp1286I G_dGCh'C
Cuts at: 0 1592 1947 1953
Size: 1592 355 6
Fragments arranged by size:
1592 355 6
BsrDI GCAATG_nn'
Cuts at: 0 340 1953
Size: 340 1613
BstBI TT'CG_AA
Cuts at: 0 1455 1953
Size: 1455 498
BstEII G'GTnAC_C
Cuts at: 0 499 1953
Size: 499 1454
BstYI r'GATC_y
Cuts at: 0 763 1291 1600 1953
Size: 763 528 309 353
Fragments arranged by size:
763 528 353 309
BtsI GCAGTG_nn'
Cuts at: 0 1053 1953
Size: 1053 900
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 150 185 1953
Size: 150 35 1768
Fragments arranged by size:
1768 150 35
DraI TTT'AAA
Cuts at: 0 1209 1953
Size: 1209 744
EaeI y'GGCC_r
Cuts at: 0 1890 1912 1953
Size: 1890 22 41
Fragments arranged by size:
1890 41 22
EarI CTCTTCn'nnn_
Cuts at: 0 770 1787 1953
Size: 770 1017 166
Fragments arranged by size:
1017 770 166
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 272 437 603 616 788 1076 1121 1133
Size: 272 165 166 13 172 288 45 12
Cuts at: 1133 1694 1888 1953
Size: 561 194 65
Fragments arranged by size:
561 288 272 194 172 166 165 65
45 13 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 731 1953
Size: 731 1222
EcoO109I rG'GnC_Cy
Cuts at: 0 721 780 1249 1522 1540 1953
Size: 721 59 469 273 18 413
Fragments arranged by size:
721 469 413 273 59 18
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1106 1953
Size: 1106 847
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 9 41 125 157 1354 1386 1953
Size: 9 32 84 32 1197 32 567
Fragments arranged by size:
1197 567 84 32 32 32 9
HincII GTy'rAC
Cuts at: 0 120 536 1953
Size: 120 416 1417
Fragments arranged by size:
1417 416 120
HindIII A'AGCT_T
Cuts at: 0 216 1391 1953
Size: 216 1175 562
Fragments arranged by size:
1175 562 216
MscI TGG'CCA
Cuts at: 0 1892 1953
Size: 1892 61
MslI CAynn'nnrTG
Cuts at: 0 560 1196 1424 1953
Size: 560 636 228 529
Fragments arranged by size:
636 560 529 228
MspA1I CmG'CkG
Cuts at: 0 1552 1953
Size: 1552 401
PpuMI rG'GwC_Cy
Cuts at: 0 721 780 1249 1522 1540 1953
Size: 721 59 469 273 18 413
Fragments arranged by size:
721 469 413 273 59 18
PsiI TTA'TAA
Cuts at: 0 1304 1953
Size: 1304 649
PspOMI G'GGCC_C
Cuts at: 0 1588 1953
Size: 1588 365
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1149 1181 1953
Size: 1149 32 772
Fragments arranged by size:
1149 772 32
PvuII CAG'CTG
Cuts at: 0 1552 1953
Size: 1552 401
ScaI AGT'ACT
Cuts at: 0 1086 1953
Size: 1086 867
SfcI C'TryA_G
Cuts at: 0 567 1295 1953
Size: 567 728 658
Fragments arranged by size:
728 658 567
SmaI CCC'GGG
Cuts at: 0 1942 1953
Size: 1942 11
SmlI C'TyrA_G
Cuts at: 0 1286 1953
Size: 1286 667
SspI AAT'ATT
Cuts at: 0 243 1574 1953
Size: 243 1331 379
Fragments arranged by size:
1331 379 243
TatI w'GTAC_w
Cuts at: 0 1084 1953
Size: 1084 869
XbaI T'CTAG_A
Cuts at: 0 1144 1953
Size: 1144 809
XmaI C'CCGG_G
Cuts at: 0 1940 1953
Size: 1940 13
Enzymes that do cut:
AcuI AhdI AlwNI ApaI ApoI AvaI BaeI BanI
BanII BbsI BglII Bme1580I BplI BpmI BpuEI BsaBI
BseRI BsmI Bsp1286I BsrDI BstBI BstEII BstYI BtsI
CspCI DraI EaeI EarI Eco57MI EcoNI EcoO109I FalI
Hin4I HincII HindIII MscI MslI MspA1I PpuMI PsiI
PspOMI PsrI PvuII ScaI SfcI SmaI SmlI SspI
TatI XbaI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AleI AlfI AloI ApaLI AscI AseI AsiSI
AvrII BamHI BbeI BbvCI BcgI BciVI BclI BfrBI
BglI BlpI BmgBI BmrI BmtI Bpu10I BsaI BsaAI
BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI BsiWI
BsmBI BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII
BssSI BstAPI BstXI BstZ17I Bsu36I BtgI BtgZI ClaI
DraIII DrdI EagI EciI EcoICRI EcoRI EcoRV FseI
FspI FspAI HaeII HpaI KasI KpnI MfeI MluI
MmeI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PasI PciI PflMI PfoI
PmeI PmlI PpiI PshAI PspXI PstI PvuI RsrII
SacI SacII SalI SanDI SapI SbfI SexAI SfiI
SfoI SgfI SgrAI SnaBI SpeI SphI SrfI StuI
StyI SwaI TaqII TstI Tth111I XcmI XhoI XmnI
ZraI